Sequence of pET100_Directional

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Sequence of pET100_Directional_TOPO

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Sequence of pET100 Directional TOPO : 5764 BP; 1358 A; 1518 C; 1568 G; 1320 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa 60

1 gttcctctac cgcgggttgt cagggggccg gtgccccgga cggtggtatg ggtgcggctt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat 120

61 tgttcgcgag tactcgggct tcaccgctcg ggctagaagg ggtagccact acagccgcta 120

.......... .......... .......... .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta 180

121 tatccgcggt cgttggcgtg gacaccgcgg ccactacggc cggtgctacg caggccgcat 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg 240

181 ctcctagctc tagagctagg gcgctttaat tatgctgagt gatatcccct taacactcgc 240

.......... .......... .......... T7_pro .......... lacO

.......... XbaI(255) .......... .......... .......... ..........

241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat atacatatgc 300

241 ctattgttaa ggggagatct ttattaaaac aaattgaaat tcttcctcta tatgtatacg 300

lacO .......... ....... T7_transl_en_RBS ....... ..........

.......... .......... .......... NheI(333) .......... ..........

301 ggggttctca tcatcatcat catcatggta tggctagcat gactggtgga cagcaaatgg 360

301 ccccaagagt agtagtagta gtagtaccat accgatcgta ctgaccacct gtcgtttacc 360

.......... 6xHis Xpress_fwd_pri T7_gene10_leader T7_leader

.......... .......... .......... .......... .......... SacI(415)

361 gtcgggatct gtacgacgat gacgataagg atcatccctt caccaagggc gagctcaacg 420

361 cagccctaga catgctgcta ctgctattcc tagtagggaa gtggttcccg ctcgagttgc 420

.......... Xpress_EK .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat 480

421 taggccgacg attgtttcgg gctttccttc gactcaaccg acgacggtgg cgactcgtta 480

.......... .......... .......... T7_term ....... T7_Terminal_pri

.......... .......... .......... .......... .......... ..........

481 aactagcata accccttggg gcctctaaac gggtcttgag gagttttttg ctgaaaggag 540

481 ttgatcgtat tggggaaccc cggagatttg cccagaactc ctcaaaaaac gactttcctc 540

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc 600

541 cttgatatag gcctataggg cgttctccgg gccgtcatgg ccgtattggt tcggatacgg 600

T7_term .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca 660

601 atgtcgtagg tcccactgcc acggctccta ctgctactcg cgtaacaatc taaagtatgt 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... HindIII(709) .........

661 cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat 720

661 gccacggact gacgcaatcg ttaaattgac actatttgat ggcgtaattt cgaatagcta 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gataagctgt caaacatgag aattaattct tgaagacgaa agggcctcgt gatacgccta 780

721 ctattcgaca gtttgtactc ttaattaaga acttctgctt tcccggagca ctatgcggat 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... AatII(823) ..........

781 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 840

781 aaaaatatcc aattacagta ctattattac caaagaatct gcagtccacc gtgaaaagcc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 900

841 cctttacacg cgccttgggg ataaacaaat aaaaagattt atgtaagttt atacataggc 900

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

901 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt 960

901 gagtactctg ttattgggac tatttacgaa gttattataa ctttttcctt ctcatactca 960

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt 1020

961 taagttgtaa aggcacagcg ggaataaggg aaaaaacgcc gtaaaacgga aggacaaaaa 1020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg 1080

1021 cgagtgggtc tttgcgacca ctttcatttt ctacgacttc tagtcaaccc acgtgctcac 1080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa 1140

1081 ccaatgtagc ttgacctaga gttgtcgcca ttctaggaac tctcaaaagc ggggcttctt 1140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt 1200

1141 gcaaaaggtt actactcgtg aaaatttcaa gacgatacac cgcgccataa tagggcacaa 1200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag 1260

1201 ctgcggcccg ttctcgttga gccagcggcg tatgtgataa gagtcttact gaaccaactc 1260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt 1320

1261 atgagtggtc agtgtctttt cgtagaatgc ctaccgtact gtcattctct taatacgtca 1320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga 1380

1321 cgacggtatt ggtactcact attgtgacgc cggttgaatg aagactgttg ctagcctcct 1380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt 1440

1381 ggcttcctcg attggcgaaa aaacgtgttg taccccctag tacattgagc ggaactagca 1440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... PstI(1500)

1441 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgca 1500

1441 acccttggcc tcgacttact tcggtatggt ttgctgctcg cactgtggtg ctacggacgt 1500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg 1560

1501 cgttaccgtt gttgcaacgc gtttgataat tgaccgcttg atgaatgaga tcgaagggcc 1560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc 1620

1561 gttgttaatt atctgaccta cctccgccta tttcaacgtc ctggtgaaga cgcgagccgg 1620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt 1680

1621 gaaggccgac cgaccaaata acgactattt agacctcggc cactcgcacc cagagcgcca 1680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg 1740

1681 tagtaacgtc gtgaccccgg tctaccattc gggagggcat agcatcaata gatgtgctgc 1740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg 1800

1741 ccctcagtcc gttgatacct acttgcttta tctgtctagc gactctatcc acggagtgac 1800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa 1860

1801 taattcgtaa ccattgacag tctggttcaa atgagtatat atgaaatcta actaaatttt 1860

ORF 1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 1920

1861 gaagtaaaaa ttaaattttc ctagatccac ttctaggaaa aactattaga gtactggttt 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 1980

1921 tagggaattg cactcaaaag caaggtgact cgcagtctgg ggcatctttt ctagtttcct 1980

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1981 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 2040

1981 agaagaactc taggaaaaaa agacgcgcat tagacgacga acgtttgttt ttttggtggc 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 2100

2041 gatggtcgcc accaaacaaa cggcctagtt ctcgatggtt gagaaaaagg cttccattga 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 2160

2101 ccgaagtcgt ctcgcgtcta tggtttatga caggaagatc acatcggcat caatccggtg 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 2220

2161 gtgaagttct tgagacatcg tggcggatgt atggagcgag acgattagga caatggtcac 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 2280

2221 cgacgacggt caccgctatt cagcacagaa tggcccaacc tgagttctgc tatcaatggc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 2340

2281 ctattccgcg tcgccagccc gacttgcccc ccaagcacgt gtgtcgggtc gaacctcgct 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 2400

2341 tgctggatgt ggcttgactc tatggatgtc gcactcgata ctctttcgcg gtgcgaaggg 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 2460

2401 cttccctctt tccgcctgtc cataggccat tcgccgtccc agccttgtcc tctcgcgtgc 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 2520

2461 tccctcgaag gtcccccttt gcggaccata gaaatatcag gacagcccaa agcggtggag 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 2580

2521 actgaactcg cagctaaaaa cactacgagc agtccccccg cctcggatac ctttttgcgg 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt 2640

2581 tcgttgcgcc ggaaaaatgc caaggaccgg aaaacgaccg gaaaacgagt gtacaagaaa 2640

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 2700

2641 ggacgcaata ggggactaag acacctattg gcataatggc ggaaactcac tcgactatgg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 2760

2701 cgagcggcgt cggcttgctg gctcgcgtcg ctcagtcact cgctccttcg ccttctcgcg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctgatgcggt attttctcct tacgcatctg tgcggtattt cacaccgcaa tggtgcactc 2820

2761 gactacgcca taaaagagga atgcgtagac acgccataaa gtgtggcgtt accacgtgag 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg 2880

2821 agtcatgtta gacgagacta cggcgtatca attcggtcat atgtgaggcg atagcgatgc 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc 2940

2881 actgacccag taccgacgcg gggctgtggg cggttgtggg cgactgcgcg ggactgcccg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg 3000

2941 aacagacgag ggccgtaggc gaatgtctgt tcgacactgg cagaggccct cgacgtacac 3000

.......... .......... .......... .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

3001 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgcggtaaa gctcatcagc 3060

3001 agtctccaaa agtggcagta gtggctttgc gcgctccgtc gacgccattt cgagtagtcg 3060

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtggtcgtga agcgattcac agatgtctgc ctgttcatcc gcgtccagct cgttgagttt 3120

3061 caccagcact tcgctaagtg tctacagacg gacaagtagg cgcaggtcga gcaactcaaa 3120

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctccagaagc gttaatgtct ggcttctgat aaagcgggcc atgttaaggg cggttttttc 3180

3121 gaggtcttcg caattacaga ccgaagacta tttcgcccgg tacaattccc gccaaaaaag 3180

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctgtttggtc actgatgcct ccgtgtaagg gggatttctg ttcatggggg taatgatacc 3240

3181 gacaaaccag tgactacgga ggcacattcc ccctaaagac aagtaccccc attactatgg 3240

ROP .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gatgaaacga gagaggatgc tcacgatacg ggttactgat gatgaacatg cccggttact 3300

3241 ctactttgct ctctcctacg agtgctatgc ccaatgacta ctacttgtac gggccaatga 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggaacgttgt gagggtaaac aactggcggt atggatgcgg cgggaccaga gaaaaatcac 3360

3301 ccttgcaaca ctcccatttg ttgaccgcca tacctacgcc gccctggtct ctttttagtg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcagggtcaa tgccagcgct tcgttaatac agatgtaggt gttccacagg gtagccagca 3420

3361 agtcccagtt acggtcgcga agcaattatg tctacatcca caaggtgtcc catcggtcgt 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gcatcctgcg atgcagatcc ggaacataat ggtgcagggc gctgacttcc gcgtttccag 3480

3421 cgtaggacgc tacgtctagg ccttgtatta ccacgtcccg cgactgaagg cgcaaaggtc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 actttacgaa acacggaaac cgaagaccat tcatgttgtt gctcaggtcg cagacgtttt 3540

3481 tgaaatgctt tgtgcctttg gcttctggta agtacaacaa cgagtccagc gtctgcaaaa 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gcagcagcag tcgcttcacg ttcgctcgcg tatcggtgat tcattctgct aaccagtaag 3600

3541 cgtcgtcgtc agcgaagtgc aagcgagcgc atagccacta agtaagacga ttggtcattc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... MscI(3660)

3601 gcaaccccgc cagcctagcc gggtcctcaa cgacaggagc acgatcatgc gcacccgtgg 3660

3601 cgttggggcg gtcggatcgg cccaggagtt gctgtcctcg tgctagtacg cgtgggcacc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ccaggaccca acgctgcccg agatgcgccg cgtgcggctg ctggagatgg cggacgcgat 3720

3661 ggtcctgggt tgcgacgggc tctacgcggc gcacgccgac gacctctacc gcctgcgcta 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggatatgttc tgccaagggt tggtttgcgc attcacagtt ctccgcaaga attgattggc 3780

3721 cctatacaag acggttccca accaaacgcg taagtgtcaa gaggcgttct taactaaccg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tccaattctt ggagtggtga atccgttagc gaggtgccgc cggcttccat tcaggtcgag 3840

3781 aggttaagaa cctcaccact taggcaatcg ctccacggcg gccgaaggta agtccagctc 3840

.......... .......... .......... .......... ....... tet(0-612) ORF 3

.......... .......... .......... .......... .......... ..........

3841 gtggcccggc tccatgcacc gcgacgcaac gcggggaggc agacaaggta tagggcggcg 3900

3841 caccgggccg aggtacgtgg cgctgcgttg cgcccctccg tctgttccat atcccgccgc 3900

.......... ....... tet(0-612) ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cctacaatcc atgccaaccc gttccatgtg ctcgccgagg cggcataaat cgccgtgacg 3960

3901 ggatgttagg tacggttggg caaggtacac gagcggctcc gccgtattta gcggcactgc 3960

.......... ....... tet(0-612) ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atcagcggtc caatgatcga agttaggctg gtaagagccg cgagcgatcc ttgaagctgt 4020

3961 tagtcgccag gttactagct tcaatccgac cattctcggc gctcgctagg aacttcgaca 4020

.......... ....... tet(0-612) ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccctgatggt cgtcatctac ctgcctggac agcatggcct gcaacgcggg catcccgatg 4080

4021 gggactacca gcagtagatg gacggacctg tcgtaccgga cgttgcgccc gtagggctac 4080

.......... ....... tet(0-612) ORF 3 ORF 3 ....... ..........

.......... .......... .......... .......... .......... ..........

4081 ccgccggaag cgagaagaat cataatgggg aaggccatcc agcctcgcgt cgcgaacgcc 4140

4081 ggcggccttc gctcttctta gtattacccc ttccggtagg tcggagcgca gcgcttgcgg 4140

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... EagI(4163) .......... .......... ..........

4141 agcaagacgt agcccagcgc gtcggccgcc atgccggcga taatggcctg cttctcgccg 4200

4141 tcgttctgca tcgggtcgcg cagccggcgg tacggccgct attaccggac gaagagcggc 4200

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aaacgtttgg tggcgggacc agtgacgaag gcttgagcga gggcgtgcaa gattccgaat 4260

4201 tttgcaaacc accgccctgg tcactgcttc cgaactcgct cccgcacgtt ctaaggctta 4260

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 accgcaagcg acaggccgat catcgtcgcg ctccagcgaa agcggtcctc gccgaaaatg 4320

4261 tggcgttcgc tgtccggcta gtagcagcgc gaggtcgctt tcgccaggag cggcttttac 4320

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 acccagagcg ctgccggcac ctgtcctacg agttgcatga taaagaagac agtcataagt 4380

4321 tgggtctcgc gacggccgtg gacaggatgc tcaacgtact atttcttctg tcagtattca 4380

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcggcgacga tagtcatgcc ccgcgcccac cggaaggagc tgactgggtt gaaggctctc 4440

4381 cgccgctgct atcagtacgg ggcgcgggtg gccttcctcg actgacccaa cttccgagag 4440

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 aagggcatcg gtcgagatcc cggtgcctaa tgagtgagct aacttacatt aattgcgttg 4500

4441 ttcccgtagc cagctctagg gccacggatt actcactcga ttgaatgtaa ttaacgcaac 4500

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc 4560

4501 gcgagtgacg ggcgaaaggt cagccctttg gacagcacgg tcgacgtaat tacttagccg 4560

.......... .......... ORF 3 ORF 1 lacI .......... ..........

.......... .......... .......... .......... .......... ..........

4561 caacgcgcgg ggagaggcgg tttgcgtatt gggcgccagg gtggtttttc ttttcaccag 4620

4561 gttgcgcgcc cctctccgcc aaacgcataa cccgcggtcc caccaaaaag aaaagtggtc 4620

.......... ....... ORF 3 ORF 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgagacgggc aacagctgat tgcccttcac cgcctggccc tgagagagtt gcagcaagcg 4680

4621 actctgcccg ttgtcgacta acgggaagtg gcggaccggg actctctcaa cgtcgttcgc 4680

.......... ....... ORF 3 ORF 1 lacI ....... .......... ..........

.......... .......... .......... .......... HpaI(4729) ..........

4681 gtccacgctg gtttgcccca gcaggcgaaa atcctgtttg atggtggtta acggcgggat 4740

4681 caggtgcgac caaacggggt cgtccgcttt taggacaaac taccaccaat tgccgcccta 4740

.......... ....... ORF 3 ORF 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ataacatgag ctgtcttcgg tatcgtcgta tcccactacc gagatatccg caccaacgcg 4800

4741 tattgtactc gacagaagcc atagcagcat agggtgatgg ctctataggc gtggttgcgc 4800

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cagcccggac tcggtaatgg cgcgcattgc gcccagcgcc atctgatcgt tggcaaccag 4860

4801 gtcgggcctg agccattacc gcgcgtaacg cgggtcgcgg tagactagca accgttggtc 4860

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 catcgcagtg ggaacgatgc cctcattcag catttgcatg gtttgttgaa aaccggacat 4920

4861 gtagcgtcac ccttgctacg ggagtaagtc gtaaacgtac caaacaactt ttggcctgta 4920

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggcactccag tcgccttccc gttccgctat cggctgaatt tgattgcgag tgagatattt 4980

4921 ccgtgaggtc agcggaaggg caaggcgata gccgacttaa actaacgctc actctataaa 4980

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... ApaI(5028) ..........

4981 atgccagcca gccagacgca gacgcgccga gacagaactt aatgggcccg ctaacagcgc 5040

4981 tacggtcggt cggtctgcgt ctgcgcggct ctgtcttgaa ttacccgggc gattgtcgcg 5040

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gatttgctgg tgacccaatg cgaccagatg ctccacgccc agtcgcgtac cgtcttcatg 5100

5041 ctaaacgacc actgggttac gctggtctac gaggtgcggg tcagcgcatg gcagaagtac 5100

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ggagaaaata atactgttga tgggtgtctg gtcagagaca tcaagaaata acgccggaac 5160

5101 cctcttttat tatgacaact acccacagac cagtctctgt agttctttat tgcggccttg 5160

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... BclI(5217)

5161 attagtgcag gcagcttcca cagcaatggc atcctggtca tccagcggat agttaatgat 5220

5161 taatcacgtc cgtcgaaggt gtcgttaccg taggaccagt aggtcgccta tcaattacta 5220

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cagcccactg acgcgttgcg cgagaagatt gtgcaccgcc gctttacagg cttcgacgcc 5280

5221 gtcgggtgac tgcgcaacgc gctcttctaa cacgtggcgg cgaaatgtcc gaagctgcgg 5280

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcttcgttct accatcgaca ccaccacgct ggcacccagt tgatcggcgc gagatttaat 5340

5281 cgaagcaaga tggtagctgt ggtggtgcga ccgtgggtca actagccgcg ctctaaatta 5340

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cgccgcgaca atttgcgacg gcgcgtgcag ggccagactg gaggtggcaa cgccaatcag 5400

5341 gcggcgctgt taaacgctgc cgcgcacgtc ccggtctgac ctccaccgtt gcggttagtc 5400

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 caacgactgt ttgcccgcca gttgttgtgc cacgcggttg ggaatgtaat tcagctccgc 5460

5401 gttgctgaca aacgggcggt caacaacacg gtgcgccaac ccttacatta agtcgaggcg 5460

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 catcgccgct tccacttttt cccgcgtttt cgcagaaacg tggctggcct ggttcaccac 5520

5461 gtagcggcga aggtgaaaaa gggcgcaaaa gcgtctttgc accgaccgga ccaagtggtg 5520

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gcgggaaacg gtctgataag agacaccggc atactctgcg acatcgtata acgttactgg 5580

5521 cgccctttgc cagactattc tctgtggccg tatgagacgc tgtagcatat tgcaatgacc 5580

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tttcacattc accaccctga attgactctc ttccgggcgc tatcatgcca taccgcgaaa 5640

5581 aaagtgtaag tggtgggact taactgagag aaggcccgcg atagtacggt atggcgcttt 5640

lacI .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ggttttgcgc cattcgatgg tgtccgggat ctcgacgctc tcccttatgc gactcctgca 5700

5641 ccaaaacgcg gtaagctacc acaggcccta gagctgcgag agggaatacg ctgaggacgt 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ttaggaagca gcccagtagt aggttgaggc cgttgagcac cgccgccgca aggaatggtg 5760

5701 aatccttcgt cgggtcatca tccaactccg gcaactcgtg gcggcggcgt tccttaccac 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 catg...... .......... .......... .......... .......... .......... 5764

5761 gtac...... .......... .......... .......... .......... .......... 5764

.......... .......... .......... .......... .......... ..........