Sequence of pET101_Directional

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Sequence of pET101_Directional_TOPO

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Sequence of pET101 Directional TOPO : 5753 BP; 1349 A; 1527 C; 1557 G; 1320 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa 60

1 gttcctctac cgcgggttgt cagggggccg gtgccccgga cggtggtatg ggtgcggctt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat 120

61 tgttcgcgag tactcgggct tcaccgctcg ggctagaagg ggtagccact acagccgcta 120

.......... .......... .......... .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta 180

121 tatccgcggt cgttggcgtg gacaccgcgg ccactacggc cggtgctacg caggccgcat 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg 240

181 ctcctagctc tagagctagg gcgctttaat tatgctgagt gatatcccct taacactcgc 240

.......... .......... .......... T7_pro .......... lacO

.......... XbaI(255) .......... .......... .......... ..........

241 gataacaatt cccctctaga aataattttg tttaacttta agaaggaatt caggagccct 300

241 ctattgttaa ggggagatct ttattaaaac aaattgaaat tcttccttaa gtcctcggga 300

lacO .......... .......... .......... .......... ..........

.......... ..... SacI(316),BstBI(321) ..... .......... ..........

301 tcaccaaggg cgagctcaat tcgaagcttg aaggtaagcc tatccctaac cctctcctcg 360

301 agtggttccc gctcgagtta agcttcgaac ttccattcgg atagggattg ggagaggagc 360

.......... .......... .......... .......... V5 ..........

.......... AgeI(378) .......... .......... .......... ..........

361 gtctcgattc tacgcgtacc ggtcatcatc accatcacca ttgagtttga tccggctgct 420

361 cagagctaag atgcgcatgg ccagtagtag tggtagtggt aactcaaact aggccgacga 420

V5 .......... .......... 6xHis .......... ..........

.......... .......... .......... .......... .......... ..........

421 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa 480

421 ttgtttcggg ctttccttcg actcaaccga cgacggtggc gactcgttat tgatcgtatt 480

.......... .......... T7_term ....... T7_Terminal_pri .......

.......... .......... .......... .......... .......... ..........

481 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc 540

481 ggggaacccc ggagatttgc ccagaactcc ccaaaaaacg actttcctcc ttgatatagg 540

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca 600

541 cctatagggc gttctccggg ccgtcatggc cgtattggtt cggatacgga tgtcgtaggt 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac 660

601 cccactgcca cggctcctac tgctactcgc gtaacaatct aaagtatgtg ccacggactg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tgcgttagca atttaactgt gataaactac cgcattaaag cttatcgatg ataagctgtc 720

661 acgcaatcgt taaattgaca ctatttgatg gcgtaatttc gaatagctac tattcgacag 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aaacatgaga attaattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt 780

721 tttgtactct taattaagaa cttctgcttt cccggagcac tatgcggata aaaatatcca 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... AatII(812) .......... ..........

781 taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg 840

781 attacagtac tattattacc aaagaatctg cagtccaccg tgaaaagccc ctttacacgc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca 900

841 gccttgggga taaacaaata aaaagattta tgtaagttta tacataggcg agtactctgt 900

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

901 ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt 960

901 tattgggact atttacgaag ttattataac tttttccttc tcatactcat aagttgtaaa 960

.......... .......... .......... .......... ....... ORF 2 Ampicillin

.......... .......... .......... .......... .......... ..........

961 ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga 1020

961 ggcacagcgg gaataaggga aaaaacgccg taaaacggaa ggacaaaaac gagtgggtct 1020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga 1080

1021 ttgcgaccac tttcattttc tacgacttct agtcaaccca cgtgctcacc caatgtagct 1080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat 1140

1081 tgacctagag ttgtcgccat tctaggaact ctcaaaagcg gggcttcttg caaaaggtta 1140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca 1200

1141 ctactcgtga aaatttcaag acgatacacc gcgccataat agggcacaac tgcggcccgt 1200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt 1260

1201 tctcgttgag ccagcggcgt atgtgataag agtcttactg aaccaactca tgagtggtca 1260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac 1320

1261 gtgtcttttc gtagaatgcc taccgtactg tcattctctt aatacgtcac gacggtattg 1320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct 1380

1321 gtactcacta ttgtgacgcc ggttgaatga agactgttgc tagcctcctg gcttcctcga 1380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga 1440

1381 ttggcgaaaa aacgtgttgt accccctagt acattgagcg gaactagcaa cccttggcct 1440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... PstI(1489) ..........

1441 gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac 1500

1441 cgacttactt cggtatggtt tgctgctcgc actgtggtgc tacggacgtc gttaccgttg 1500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat 1560

1501 ttgcaacgcg tttgataatt gaccgcttga tgaatgagat cgaagggccg ttgttaatta 1560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg 1620

1561 tctgacctac ctccgcctat ttcaacgtcc tggtgaagac gcgagccggg aaggccgacc 1620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc 1680

1621 gaccaaataa cgactattta gacctcggcc actcgcaccc agagcgccat agtaacgtcg 1680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc 1740

1681 tgaccccggt ctaccattcg ggagggcata gcatcaatag atgtgctgcc cctcagtccg 1740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg 1800

1741 ttgataccta cttgctttat ctgtctagcg actctatcca cggagtgact aattcgtaac 1800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta 1860

1801 cattgacagt ctggttcaaa tgagtatata tgaaatctaa ctaaattttg aagtaaaaat 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg 1920

1861 taaattttcc tagatccact tctaggaaaa actattagag tactggtttt agggaattgc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga 1980

1921 actcaaaagc aaggtgactc gcagtctggg gcatcttttc tagtttccta gaagaactct 1980

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1981 tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt 2040

1981 aggaaaaaaa gacgcgcatt agacgacgaa cgtttgtttt tttggtggcg atggtcgcca 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag 2100

2041 ccaaacaaac ggcctagttc tcgatggttg agaaaaaggc ttccattgac cgaagtcgtc 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa 2160

2101 tcgcgtctat ggtttatgac aggaagatca catcggcatc aatccggtgg tgaagttctt 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag 2220

2161 gagacatcgt ggcggatgta tggagcgaga cgattaggac aatggtcacc gacgacggtc 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca 2280

2221 accgctattc agcacagaat ggcccaacct gagttctgct atcaatggcc tattccgcgt 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac 2340

2281 cgccagcccg acttgccccc caagcacgtg tgtcgggtcg aacctcgctt gctggatgtg 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa 2400

2341 gcttgactct atggatgtcg cactcgatac tctttcgcgg tgcgaagggc ttccctcttt 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc 2460

2401 ccgcctgtcc ataggccatt cgccgtccca gccttgtcct ctcgcgtgct ccctcgaagg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg 2520

2461 tccccctttg cggaccatag aaatatcagg acagcccaaa gcggtggaga ctgaactcgc 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc 2580

2521 agctaaaaac actacgagca gtccccccgc ctcggatacc tttttgcggt cgttgcgccg 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc 2640

2581 gaaaaatgcc aaggaccgga aaacgaccgg aaaacgagtg tacaagaaag gacgcaatag 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag 2700

2641 gggactaaga cacctattgg cataatggcg gaaactcact cgactatggc gagcggcgtc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta 2760

2701 ggcttgctgg ctcgcgtcgc tcagtcactc gctccttcgc cttctcgcgg actacgccat 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ttttctcctt acgcatctgt gcggtatttc acaccgcaat ggtgcactct cagtacaatc 2820

2761 aaaagaggaa tgcgtagaca cgccataaag tgtggcgtta ccacgtgaga gtcatgttag 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tgctctgatg ccgcatagtt aagccagtat acactccgct atcgctacgt gactgggtca 2880

2821 acgagactac ggcgtatcaa ttcggtcata tgtgaggcga tagcgatgca ctgacccagt 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc 2940

2881 accgacgcgg ggctgtgggc ggttgtgggc gactgcgcgg gactgcccga acagacgagg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt 3000

2941 gccgtaggcg aatgtctgtt cgacactggc agaggccctc gacgtacaca gtctccaaaa 3000

.......... .......... .......... .......... pGEX_3_pri ROP

.......... .......... .......... .......... .......... ..........

3001 caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag ctcatcagcg tggtcgtgaa 3060

3001 gtggcagtag tggctttgcg cgctccgtcg acgccatttc gagtagtcgc accagcactt 3060

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gcgattcaca gatgtctgcc tgttcatccg cgtccagctc gttgagtttc tccagaagcg 3120

3061 cgctaagtgt ctacagacgg acaagtaggc gcaggtcgag caactcaaag aggtcttcgc 3120

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttaatgtctg gcttctgata aagcgggcca tgttaagggc ggttttttcc tgtttggtca 3180

3121 aattacagac cgaagactat ttcgcccggt acaattcccg ccaaaaaagg acaaaccagt 3180

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt aatgataccg atgaaacgag 3240

3181 gactacggag gcacattccc cctaaagaca agtaccccca ttactatggc tactttgctc 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 agaggatgct cacgatacgg gttactgatg atgaacatgc ccggttactg gaacgttgtg 3300

3241 tctcctacga gtgctatgcc caatgactac tacttgtacg ggccaatgac cttgcaacac 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agggtaaaca actggcggta tggatgcggc gggaccagag aaaaatcact cagggtcaat 3360

3301 tcccatttgt tgaccgccat acctacgccg ccctggtctc tttttagtga gtcccagtta 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gccagcgctt cgttaataca gatgtaggtg ttccacaggg tagccagcag catcctgcga 3420

3361 cggtcgcgaa gcaattatgt ctacatccac aaggtgtccc atcggtcgtc gtaggacgct 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgcagatccg gaacataatg gtgcagggcg ctgacttccg cgtttccaga ctttacgaaa 3480

3421 acgtctaggc cttgtattac cacgtcccgc gactgaaggc gcaaaggtct gaaatgcttt 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cacggaaacc gaagaccatt catgttgttg ctcaggtcgc agacgttttg cagcagcagt 3540

3481 gtgcctttgg cttctggtaa gtacaacaac gagtccagcg tctgcaaaac gtcgtcgtca 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta accagtaagg caaccccgcc 3600

3541 gcgaagtgca agcgagcgca tagccactaa gtaagacgat tggtcattcc gttggggcgg 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... MscI(3649) ..........

3601 agcctagccg ggtcctcaac gacaggagca cgatcatgcg cacccgtggc caggacccaa 3660

3601 tcggatcggc ccaggagttg ctgtcctcgt gctagtacgc gtgggcaccg gtcctgggtt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgctgcccga gatgcgccgc gtgcggctgc tggagatggc ggacgcgatg gatatgttct 3720

3661 gcgacgggct ctacgcggcg cacgccgacg acctctaccg cctgcgctac ctatacaaga 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa ttgattggct ccaattcttg 3780

3721 cggttcccaa ccaaacgcgt aagtgtcaag aggcgttctt aactaaccga ggttaagaac 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt caggtcgagg tggcccggct 3840

3781 ctcaccactt aggcaatcgc tccacggcgg ccgaaggtaa gtccagctcc accgggccga 3840

.......... .......... .......... ....... tet(0-612) ORF 1 .......

.......... .......... .......... .......... .......... ..........

3841 ccatgcaccg cgacgcaacg cggggaggca gacaaggtat agggcggcgc ctacaatcca 3900

3841 ggtacgtggc gctgcgttgc gcccctccgt ctgttccata tcccgccgcg gatgttaggt 3900

.......... ....... tet(0-612) ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc gccgtgacga tcagcggtcc 3960

3901 acggttgggc aaggtacacg agcggctccg ccgtatttag cggcactgct agtcgccagg 3960

.......... ....... tet(0-612) ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 aatgatcgaa gttaggctgg taagagccgc gagcgatcct tgaagctgtc cctgatggtc 4020

3961 ttactagctt caatccgacc attctcggcg ctcgctagga acttcgacag ggactaccag 4020

.......... ....... tet(0-612) ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gtcatctacc tgcctggaca gcatggcctg caacgcgggc atcccgatgc cgccggaagc 4080

4021 cagtagatgg acggacctgt cgtaccggac gttgcgcccg tagggctacg gcggccttcg 4080

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gagaagaatc ataatgggga aggccatcca gcctcgcgtc gcgaacgcca gcaagacgta 4140

4081 ctcttcttag tattacccct tccggtaggt cggagcgcag cgcttgcggt cgttctgcat 4140

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... EagI(4152) .......... .......... .......... ..........

4141 gcccagcgcg tcggccgcca tgccggcgat aatggcctgc ttctcgccga aacgtttggt 4200

4141 cgggtcgcgc agccggcggt acggccgcta ttaccggacg aagagcggct ttgcaaacca 4200

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag attccgaata ccgcaagcga 4260

4201 ccgccctggt cactgcttcc gaactcgctc ccgcacgttc taaggcttat ggcgttcgct 4260

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg ccgaaaatga cccagagcgc 4320

4261 gtccggctag tagcagcgcg aggtcgcttt cgccaggagc ggcttttact gggtctcgcg 4320

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tgccggcacc tgtcctacga gttgcatgat aaagaagaca gtcataagtg cggcgacgat 4380

4321 acggccgtgg acaggatgct caacgtacta tttcttctgt cagtattcac gccgctgcta 4380

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agtcatgccc cgcgcccacc ggaaggagct gactgggttg aaggctctca agggcatcgg 4440

4381 tcagtacggg gcgcgggtgg ccttcctcga ctgacccaac ttccgagagt tcccgtagcc 4440

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcgagatccc ggtgcctaat gagtgagcta acttacatta attgcgttgc gctcactgcc 4500

4441 agctctaggg ccacggatta ctcactcgat tgaatgtaat taacgcaacg cgagtgacgg 4500

.......... .......... ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg 4560

4501 gcgaaaggtc agccctttgg acagcacggt cgacgtaatt acttagccgg ttgcgcgccc 4560

.......... ....... ORF 1 ORF 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gagaggcggt ttgcgtattg ggcgccaggg tggtttttct tttcaccagt gagacgggca 4620

4561 ctctccgcca aacgcataac ccgcggtccc accaaaaaga aaagtggtca ctctgcccgt 4620

.......... ....... ORF 1 ORF 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acagctgatt gcccttcacc gcctggccct gagagagttg cagcaagcgg tccacgctgg 4680

4621 tgtcgactaa cgggaagtgg cggaccggga ctctctcaac gtcgttcgcc aggtgcgacc 4680

.......... ....... ORF 1 ORF 2 lacI ....... .......... ..........

.......... .......... .......... HpaI(4718) .......... ..........

4681 tttgccccag caggcgaaaa tcctgtttga tggtggttaa cggcgggata taacatgagc 4740

4681 aaacggggtc gtccgctttt aggacaaact accaccaatt gccgccctat attgtactcg 4740

.......... ORF 1 ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tgtcttcggt atcgtcgtat cccactaccg agatatccgc accaacgcgc agcccggact 4800

4741 acagaagcca tagcagcata gggtgatggc tctataggcg tggttgcgcg tcgggcctga 4800

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 cggtaatggc gcgcattgcg cccagcgcca tctgatcgtt ggcaaccagc atcgcagtgg 4860

4801 gccattaccg cgcgtaacgc gggtcgcggt agactagcaa ccgttggtcg tagcgtcacc 4860

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gaacgatgcc ctcattcagc atttgcatgg tttgttgaaa accggacatg gcactccagt 4920

4861 cttgctacgg gagtaagtcg taaacgtacc aaacaacttt tggcctgtac cgtgaggtca 4920

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cgccttcccg ttccgctatc ggctgaattt gattgcgagt gagatattta tgccagccag 4980

4921 gcggaagggc aaggcgatag ccgacttaaa ctaacgctca ctctataaat acggtcggtc 4980

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... ApaI(5017) .......... ..........

4981 ccagacgcag acgcgccgag acagaactta atgggcccgc taacagcgcg atttgctggt 5040

4981 ggtctgcgtc tgcgcggctc tgtcttgaat tacccgggcg attgtcgcgc taaacgacca 5040

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gacccaatgc gaccagatgc tccacgccca gtcgcgtacc gtcttcatgg gagaaaataa 5100

5041 ctgggttacg ctggtctacg aggtgcgggt cagcgcatgg cagaagtacc ctcttttatt 5100

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tactgttgat gggtgtctgg tcagagacat caagaaataa cgccggaaca ttagtgcagg 5160

5101 atgacaacta cccacagacc agtctctgta gttctttatt gcggccttgt aatcacgtcc 5160

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... BclI(5206) ..........

5161 cagcttccac agcaatggca tcctggtcat ccagcggata gttaatgatc agcccactga 5220

5161 gtcgaaggtg tcgttaccgt aggaccagta ggtcgcctat caattactag tcgggtgact 5220

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cgcgttgcgc gagaagattg tgcaccgccg ctttacaggc ttcgacgccg cttcgttcta 5280

5221 gcgcaacgcg ctcttctaac acgtggcggc gaaatgtccg aagctgcggc gaagcaagat 5280

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ccatcgacac caccacgctg gcacccagtt gatcggcgcg agatttaatc gccgcgacaa 5340

5281 ggtagctgtg gtggtgcgac cgtgggtcaa ctagccgcgc tctaaattag cggcgctgtt 5340

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tttgcgacgg cgcgtgcagg gccagactgg aggtggcaac gccaatcagc aacgactgtt 5400

5341 aaacgctgcc gcgcacgtcc cggtctgacc tccaccgttg cggttagtcg ttgctgacaa 5400

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tgcccgccag ttgttgtgcc acgcggttgg gaatgtaatt cagctccgcc atcgccgctt 5460

5401 acgggcggtc aacaacacgg tgcgccaacc cttacattaa gtcgaggcgg tagcggcgaa 5460

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ccactttttc ccgcgttttc gcagaaacgt ggctggcctg gttcaccacg cgggaaacgg 5520

5461 ggtgaaaaag ggcgcaaaag cgtctttgca ccgaccggac caagtggtgc gccctttgcc 5520

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 tctgataaga gacaccggca tactctgcga catcgtataa cgttactggt ttcacattca 5580

5521 agactattct ctgtggccgt atgagacgct gtagcatatt gcaatgacca aagtgtaagt 5580

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ccaccctgaa ttgactctct tccgggcgct atcatgccat accgcgaaag gttttgcgcc 5640

5581 ggtgggactt aactgagaga aggcccgcga tagtacggta tggcgctttc caaaacgcgg 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 attcgatggt gtccgggatc tcgacgctct cccttatgcg actcctgcat taggaagcag 5700

5641 taagctacca caggccctag agctgcgaga gggaatacgc tgaggacgta atccttcgtc 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atg....... 5753

5701 gggtcatcat ccaactccgg caactcgtgg cggcggcgtt ccttaccacg tac....... 5753

.......... .......... .......... .......... .......... ..........