Sequence of pET102_Directional

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Sequence of pET102_Directional_TOPO

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Sequence of pET102 Directional TOPO : 6315 BP; 1494 A; 1655 C; 1721 G; 1445 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa 60

1 gttcctctac cgcgggttgt cagggggccg gtgccccgga cggtggtatg ggtgcggctt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat 120

61 tgttcgcgag tactcgggct tcaccgctcg ggctagaagg ggtagccact acagccgcta 120

.......... .......... .......... .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta 180

121 tatccgcggt cgttggcgtg gacaccgcgg ccactacggc cggtgctacg caggccgcat 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg 240

181 ctcctagctc tagagctagg gcgctttaat tatgctgagt gatatcccct taacactcgc 240

.......... .......... .......... T7_pro .......... lacO

.......... XbaI(255) .......... .......... .......... ..........

241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat atacataatg 300

241 ctattgttaa ggggagatct ttattaaaac aaattgaaat tcttcctcta tatgtattac 300

lacO .......... ....... T7_transl_en_RBS ....... ..........

.......... .......... .......... .......... .......... ..........

301 ggatctgata aaattattca tctgactgat gattcttttg atactgatgt acttaaggca 360

301 cctagactat tttaataagt agactgacta ctaagaaaac tatgactaca tgaattccgt 360

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

361 gatggtgcaa tcctggttga tttctgggca cactggtgcg gtccgtgcaa aatgatcgct 420

361 ctaccacgtt aggaccaact aaagacccgt gtgaccacgc caggcacgtt ttactagcga 420

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 ccgattctgg atgaaatcgc tgacgaatat cagggcaaac tgaccgttgc aaaactgaac 480

421 ggctaagacc tactttagcg actgcttata gtcccgtttg actggcaacg ttttgacttg 480

.......... ........ His_patch_thio ........ .......... ..........

.......... SmaI(494) .......... .......... .......... ..........

481 atcgatcaca acccgggcac tgcgccgaaa tatggcatcc gtggtatccc gactctgctg 540

481 tagctagtgt tgggcccgtg acgcggcttt ataccgtagg caccataggg ctgagacgac 540

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 ctgttcaaaa acggtgaagt ggcggcaacc aaagtgggtg cactgtctaa aggtcagttg 600

541 gacaagtttt tgccacttca ccgccgttgg tttcacccac gtgacagatt tccagtcaac 600

.......... ........ His_patch_thio ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 aaagagttcc tcgacgctaa cctggccggc tctggatccg gtgatgacga tgacaagctg 660

601 tttctcaagg agctgcgatt ggaccggccg agacctaggc cactactgct actgttcgac 660

........ His_patch_thio ........ .......... EK ..........

.......... .......... .......... .......... .......... ..........

661 ggaattgatc ccttcaccaa gggcgagctc aagcttgaag gtaagcctat ccctaaccct 720

661 ccttaactag ggaagtggtt cccgctcgag ttcgaacttc cattcggata gggattggga 720

.......... .......... .......... .......... V5 ..........

.......... .......... AgeI(745) .......... .......... ..........

721 ctcctcggtc tcgattctac gcgtaccggt catcatcacc atcaccattg agtttgatcc 780

721 gaggagccag agctaagatg cgcatggcca gtagtagtgg tagtggtaac tcaaactagg 780

.......... V5 .......... 6xHis .......... ..........

.......... .......... .......... .......... .......... ..........

781 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact 840

781 ccgacgattg tttcgggctt tccttcgact caaccgacga cggtggcgac tcgttattga 840

.......... .......... .......... T7_term ....... T7_Terminal_pri

.......... .......... .......... .......... .......... ..........

841 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac 900

841 tcgtattggg gaaccccgga gatttgccca gaactcccca aaaaacgact ttcctccttg 900

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tatatccgga tatccacagg acgggtgtgg tcgccatgat cgcgtagtcg atagtggctc 960

901 atataggcct ataggtgtcc tgcccacacc agcggtacta gcgcatcagc tatcaccgag 960

T7_term .......... ........ tet(900-1191) ........ ..........

.......... .......... .......... .......... .......... ..........

961 caagtagcga agcgagcagg actgggcggc ggccaaagcg gtcggacagt gctccgagaa 1020

961 gttcatcgct tcgctcgtcc tgacccgccg ccggtttcgc cagcctgtca cgaggctctt 1020

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cgggtgcgca tagaaattgc atcaacgcat atagcgctag cagcacgcca tagtgactgg 1080

1021 gcccacgcgt atctttaacg tagttgcgta tatcgcgatc gtcgtgcggt atcactgacc 1080

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cgatgctgtc ggaatggacg atatcccgca agaggcccgg cagtaccggc ataaccaagc 1140

1081 gctacgacag ccttacctgc tatagggcgt tctccgggcc gtcatggccg tattggttcg 1140

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ctatgcctac agcatccagg gtgacggtgc cgaggatgac gatgagcgca ttgttagatt 1200

1141 gatacggatg tcgtaggtcc cactgccacg gctcctactg ctactcgcgt aacaatctaa 1200

.......... ........ tet(900-1191) ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tcatacacgg tgcctgactg cgttagcaat ttaactgtga taaactaccg cattaaagct 1260

1201 agtatgtgcc acggactgac gcaatcgtta aattgacact atttgatggc gtaatttcga 1260

.......... .......... ......... tet(w/ gaps) ......... ..........

.......... .......... .......... .......... .......... ..........

1261 agcttatcga tgataagctg tcaaacatga gaattaattc ttgaagacga aagggcctcg 1320

1261 tcgaatagct actattcgac agtttgtact cttaattaag aacttctgct ttcccggagc 1320

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... ......... AatII(1374)

1321 tgatacgcct atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg 1380

1321 actatgcgga taaaaatatc caattacagt actattatta ccaaagaatc tgcagtccac 1380

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 1440

1381 cgtgaaaagc ccctttacac gcgccttggg gataaacaaa taaaaagatt tatgtaagtt 1440

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 1500

1441 tatacatagg cgagtactct gttattggga ctatttacga agttattata actttttcct 1500

......... AmpR_pro tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 1560

1501 tctcatactc ataagttgta aaggcacagc gggaataagg gaaaaaacgc cgtaaaacgg 1560

.......... ...... tet(w/ gaps) ORF 3 Ampicillin ...... ..........

.......... .......... .......... .......... .......... ..........

1561 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 1620

1561 aaggacaaaa acgagtgggt ctttgcgacc actttcattt tctacgactt ctagtcaacc 1620

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 1680

1621 cacgtgctca cccaatgtag cttgacctag agttgtcgcc attctaggaa ctctcaaaag 1680

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 1740

1681 cggggcttct tgcaaaaggt tactactcgt gaaaatttca agacgataca ccgcgccata 1740

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 1800

1741 atagggcaca actgcggccc gttctcgttg agccagcggc gtatgtgata agagtcttac 1800

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

1801 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 1860

1801 tgaaccaact catgagtggt cagtgtcttt tcgtagaatg cctaccgtac tgtcattctc 1860

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 1920

1861 ttaatacgtc acgacggtat tggtactcac tattgtgacg ccggttgaat gaagactgtt 1920

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 1980

1921 gctagcctcc tggcttcctc gattggcgaa aaaacgtgtt gtacccccta gtacattgag 1980

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 2040

1981 cggaactagc aacccttggc ctcgacttac ttcggtatgg tttgctgctc gcactgtggt 2040

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... PstI(2051) .......... .......... .......... ..........

2041 cgatgcctgc agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 2100

2041 gctacggacg tcgttaccgt tgttgcaacg cgtttgataa ttgaccgctt gatgaatgag 2100

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 2160

2101 atcgaagggc cgttgttaat tatctgacct acctccgcct atttcaacgt cctggtgaag 2160

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 2220

2161 acgcgagccg ggaaggccga ccgaccaaat aacgactatt tagacctcgg ccactcgcac 2220

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 2280

2221 ccagagcgcc atagtaacgt cgtgaccccg gtctaccatt cgggagggca tagcatcaat 2280

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 2340

2281 agatgtgctg cccctcagtc cgttgatacc tacttgcttt atctgtctag cgactctatc 2340

.......... tet(w/ gaps) ORF 3 Ampicillin .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 2400

2341 cacggagtga ctaattcgta accattgaca gtctggttca aatgagtata tatgaaatct 2400

...... ORF 3 Ampicillin tet(w/ gaps) ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 2460

2401 aactaaattt tgaagtaaaa attaaatttt cctagatcca cttctaggaa aaactattag 2460

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 2520

2461 agtactggtt ttagggaatt gcactcaaaa gcaaggtgac tcgcagtctg gggcatcttt 2520

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 2580

2521 tctagtttcc tagaagaact ctaggaaaaa aagacgcgca ttagacgacg aacgtttgtt 2580

.......... .. tet(w/ gaps) pBR322_origin .. .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 2640

2581 tttttggtgg cgatggtcgc caccaaacaa acggcctagt tctcgatggt tgagaaaaag 2640

.......... .. tet(w/ gaps) pBR322_origin .. .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt 2700

2641 gcttccattg accgaagtcg tctcgcgtct atggtttatg acaggaagat cacatcggca 2700

.......... .. tet(w/ gaps) pBR322_origin .. .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 2760

2701 tcaatccggt ggtgaagttc ttgagacatc gtggcggatg tatggagcga gacgattagg 2760

.......... .. tet(w/ gaps) pBR322_origin .. .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 2820

2761 acaatggtca ccgacgacgg tcaccgctat tcagcacaga atggcccaac ctgagttctg 2820

.......... .. tet(w/ gaps) pBR322_origin .. .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 2880

2821 ctatcaatgg cctattccgc gtcgccagcc cgacttgccc cccaagcacg tgtgtcgggt 2880

.......... .. tet(w/ gaps) pBR322_origin .. .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 2940

2881 cgaacctcgc ttgctggatg tggcttgact ctatggatgt cgcactcgat actctttcgc 2940

.......... .. tet(w/ gaps) pBR322_origin .. .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 3000

2941 ggtgcgaagg gcttccctct ttccgcctgt ccataggcca ttcgccgtcc cagccttgtc 3000

.......... .. tet(w/ gaps) pBR322_origin .. .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 3060

3001 ctctcgcgtg ctccctcgaa ggtccccctt tgcggaccat agaaatatca ggacagccca 3060

.......... .. tet(w/ gaps) pBR322_origin .. .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 3120

3061 aagcggtgga gactgaactc gcagctaaaa acactacgag cagtcccccc gcctcggata 3120

.......... .. tet(w/ gaps) pBR322_origin .. .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 3180

3121 cctttttgcg gtcgttgcgc cggaaaaatg ccaaggaccg gaaaacgacc ggaaaacgag 3180

pBR322_origin ..... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 3240

3181 tgtacaagaa aggacgcaat aggggactaa gacacctatt ggcataatgg cggaaactca 3240

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 3300

3241 ctcgactatg gcgagcggcg tcggcttgct ggctcgcgtc gctcagtcac tcgctccttc 3300

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca 3360

3301 gccttctcgc ggactacgcc ataaaagagg aatgcgtaga cacgccataa agtgtggcgt 3360

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atacactccg 3420

3361 taccacgtga gagtcatgtt agacgagact acggcgtatc aattcggtca tatgtgaggc 3420

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctatcgctac gtgactgggt catggctgcg ccccgacacc cgccaacacc cgctgacgcg 3480

3421 gatagcgatg cactgaccca gtaccgacgc ggggctgtgg gcggttgtgg gcgactgcgc 3480

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccctgacggg cttgtctgct cccggcatcc gcttacagac aagctgtgac cgtctccggg 3540

3481 gggactgccc gaacagacga gggccgtagg cgaatgtctg ttcgacactg gcagaggccc 3540

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac gcgcgaggca gctgcggtaa 3600

3541 tcgacgtaca cagtctccaa aagtggcagt agtggctttg cgcgctccgt cgacgccatt 3600

pGEX_3_pri ......... tet(w/ gaps) ROP ......... ..........

.......... .......... .......... .......... .......... ..........

3601 agctcatcag cgtggtcgtg aagcgattca cagatgtctg cctgttcatc cgcgtccagc 3660

3601 tcgagtagtc gcaccagcac ttcgctaagt gtctacagac ggacaagtag gcgcaggtcg 3660

.......... ....... tet(w/ gaps) ROP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tcgttgagtt tctccagaag cgttaatgtc tggcttctga taaagcgggc catgttaagg 3720

3661 agcaactcaa agaggtcttc gcaattacag accgaagact atttcgcccg gtacaattcc 3720

.......... ....... tet(w/ gaps) ROP ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gcggtttttt cctgtttggt cactgatgcc tccgtgtaag ggggatttct gttcatgggg 3780

3721 cgccaaaaaa ggacaaacca gtgactacgg aggcacattc cccctaaaga caagtacccc 3780

......... ROP tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtaatgatac cgatgaaacg agagaggatg ctcacgatac gggttactga tgatgaacat 3840

3781 cattactatg gctactttgc tctctcctac gagtgctatg cccaatgact actacttgta 3840

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcccggttac tggaacgttg tgagggtaaa caactggcgg tatggatgcg gcgggaccag 3900

3841 cgggccaatg accttgcaac actcccattt gttgaccgcc atacctacgc cgccctggtc 3900

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agaaaaatca ctcagggtca atgccagcgc ttcgttaata cagatgtagg tgttccacag 3960

3901 tctttttagt gagtcccagt tacggtcgcg aagcaattat gtctacatcc acaaggtgtc 3960

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ggtagccagc agcatcctgc gatgcagatc cggaacataa tggtgcaggg cgctgacttc 4020

3961 ccatcggtcg tcgtaggacg ctacgtctag gccttgtatt accacgtccc gcgactgaag 4020

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cgcgtttcca gactttacga aacacggaaa ccgaagacca ttcatgttgt tgctcaggtc 4080

4021 gcgcaaaggt ctgaaatgct ttgtgccttt ggcttctggt aagtacaaca acgagtccag 4080

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gcagacgttt tgcagcagca gtcgcttcac gttcgctcgc gtatcggtga ttcattctgc 4140

4081 cgtctgcaaa acgtcgtcgt cagcgaagtg caagcgagcg catagccact aagtaagacg 4140

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 taaccagtaa ggcaaccccg ccagcctagc cgggtcctca acgacaggag cacgatcatg 4200

4141 attggtcatt ccgttggggc ggtcggatcg gcccaggagt tgctgtcctc gtgctagtac 4200

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... MscI(4211) .......... .......... .......... ..........

4201 cgcacccgtg gccaggaccc aacgctgccc gagatgcgcc gcgtgcggct gctggagatg 4260

4201 gcgtgggcac cggtcctggg ttgcgacggg ctctacgcgg cgcacgccga cgacctctac 4260

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gcggacgcga tggatatgtt ctgccaaggg ttggtttgcg cattcacagt tctccgcaag 4320

4261 cgcctgcgct acctatacaa gacggttccc aaccaaacgc gtaagtgtca agaggcgttc 4320

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aattgattgg ctccaattct tggagtggtg aatccgttag cgaggtgccg ccggcttcca 4380

4321 ttaactaacc gaggttaaga acctcaccac ttaggcaatc gctccacggc ggccgaaggt 4380

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ttcaggtcga ggtggcccgg ctccatgcac cgcgacgcaa cgcggggagg cagacaaggt 4440

4381 aagtccagct ccaccgggcc gaggtacgtg gcgctgcgtt gcgcccctcc gtctgttcca 4440

.......... .......... .......... ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

4441 atagggcggc gcctacaatc catgccaacc cgttccatgt gctcgccgag gcggcataaa 4500

4441 tatcccgccg cggatgttag gtacggttgg gcaaggtaca cgagcggctc cgccgtattt 4500

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tcgccgtgac gatcagcggt ccaatgatcg aagttaggct ggtaagagcc gcgagcgatc 4560

4501 agcggcactg ctagtcgcca ggttactagc ttcaatccga ccattctcgg cgctcgctag 4560

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cttgaagctg tccctgatgg tcgtcatcta cctgcctgga cagcatggcc tgcaacgcgg 4620

4561 gaacttcgac agggactacc agcagtagat ggacggacct gtcgtaccgg acgttgcgcc 4620

.......... .......... ORF 2 ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gcatcccgat gccgccggaa gcgagaagaa tcataatggg gaaggccatc cagcctcgcg 4680

4621 cgtagggcta cggcggcctt cgctcttctt agtattaccc cttccggtag gtcggagcgc 4680

.......... ......... ORF 2 ORF 2 ......... .......... ..........

NruI(4683) .......... .......... EagI(4714) .......... ..........

4681 tcgcgaacgc cagcaagacg tagcccagcg cgtcggccgc catgccggcg ataatggcct 4740

4681 agcgcttgcg gtcgttctgc atcgggtcgc gcagccggcg gtacggccgc tattaccgga 4740

.......... ......... ORF 2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gcttctcgcc gaaacgtttg gtggcgggac cagtgacgaa ggcttgagcg agggcgtgca 4800

4741 cgaagagcgg ctttgcaaac caccgccctg gtcactgctt ccgaactcgc tcccgcacgt 4800

.......... ......... ORF 2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 agattccgaa taccgcaagc gacaggccga tcatcgtcgc gctccagcga aagcggtcct 4860

4801 tctaaggctt atggcgttcg ctgtccggct agtagcagcg cgaggtcgct ttcgccagga 4860

.......... ......... ORF 2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cgccgaaaat gacccagagc gctgccggca cctgtcctac gagttgcatg ataaagaaga 4920

4861 gcggctttta ctgggtctcg cgacggccgt ggacaggatg ctcaacgtac tatttcttct 4920

.......... ......... ORF 2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cagtcataag tgcggcgacg atagtcatgc cccgcgccca ccggaaggag ctgactgggt 4980

4921 gtcagtattc acgccgctgc tatcagtacg gggcgcgggt ggccttcctc gactgaccca 4980

.......... ......... ORF 2 ORF 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tgaaggctct caagggcatc ggtcgagatc ccggtgccta atgagtgagc taacttacat 5040

4981 acttccgaga gttcccgtag ccagctctag ggccacggat tactcactcg attgaatgta 5040

ORF 2 .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt 5100

5041 attaacgcaa cgcgagtgac gggcgaaagg tcagcccttt ggacagcacg gtcgacgtaa 5100

.......... .......... ORF 2 ORF 3 lacI .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgccag ggtggttttt 5160

5101 ttacttagcc ggttgcgcgc ccctctccgc caaacgcata acccgcggtc ccaccaaaaa 5160

.......... ....... ORF 2 ORF 3 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cttttcacca gtgagacggg caacagctga ttgcccttca ccgcctggcc ctgagagagt 5220

5161 gaaaagtggt cactctgccc gttgtcgact aacgggaagt ggcggaccgg gactctctca 5220

.......... ....... ORF 2 ORF 3 lacI ....... .......... ..........

.......... .......... .......... .......... .......... HpaI(5280)

5221 tgcagcaagc ggtccacgct ggtttgcccc agcaggcgaa aatcctgttt gatggtggtt 5280

5221 acgtcgttcg ccaggtgcga ccaaacgggg tcgtccgctt ttaggacaaa ctaccaccaa 5280

.......... ....... ORF 2 ORF 3 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 aacggcggga tataacatga gctgtcttcg gtatcgtcgt atcccactac cgagatatcc 5340

5281 ttgccgccct atattgtact cgacagaagc catagcagca tagggtgatg gctctatagg 5340

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcaccaacgc gcagcccgga ctcggtaatg gcgcgcattg cgcccagcgc catctgatcg 5400

5341 cgtggttgcg cgtcgggcct gagccattac cgcgcgtaac gcgggtcgcg gtagactagc 5400

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ttggcaacca gcatcgcagt gggaacgatg ccctcattca gcatttgcat ggtttgttga 5460

5401 aaccgttggt cgtagcgtca cccttgctac gggagtaagt cgtaaacgta ccaaacaact 5460

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aaaccggaca tggcactcca gtcgccttcc cgttccgcta tcggctgaat ttgattgcga 5520

5461 tttggcctgt accgtgaggt cagcggaagg gcaaggcgat agccgactta aactaacgct 5520

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ApaI(5579)

5521 gtgagatatt tatgccagcc agccagacgc agacgcgccg agacagaact taatgggccc 5580

5521 cactctataa atacggtcgg tcggtctgcg tctgcgcggc tctgtcttga attacccggg 5580

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gctaacagcg cgatttgctg gtgacccaat gcgaccagat gctccacgcc cagtcgcgta 5640

5581 cgattgtcgc gctaaacgac cactgggtta cgctggtcta cgaggtgcgg gtcagcgcat 5640

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ccgtcttcat gggagaaaat aatactgttg atgggtgtct ggtcagagac atcaagaaat 5700

5641 ggcagaagta ccctctttta ttatgacaac tacccacaga ccagtctctg tagttcttta 5700

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 aacgccggaa cattagtgca ggcagcttcc acagcaatgg catcctggtc atccagcgga 5760

5701 ttgcggcctt gtaatcacgt ccgtcgaagg tgtcgttacc gtaggaccag taggtcgcct 5760

.......... .......... ORF 3 lacI .......... .......... ..........

BclI(5768) .......... .......... .......... .......... ..........

5761 tagttaatga tcagcccact gacgcgttgc gcgagaagat tgtgcaccgc cgctttacag 5820

5761 atcaattact agtcgggtga ctgcgcaacg cgctcttcta acacgtggcg gcgaaatgtc 5820

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 gcttcgacgc cgcttcgttc taccatcgac accaccacgc tggcacccag ttgatcggcg 5880

5821 cgaagctgcg gcgaagcaag atggtagctg tggtggtgcg accgtgggtc aactagccgc 5880

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 cgagatttaa tcgccgcgac aatttgcgac ggcgcgtgca gggccagact ggaggtggca 5940

5881 gctctaaatt agcggcgctg ttaaacgctg ccgcgcacgt cccggtctga cctccaccgt 5940

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 acgccaatca gcaacgactg tttgcccgcc agttgttgtg ccacgcggtt gggaatgtaa 6000

5941 tgcggttagt cgttgctgac aaacgggcgg tcaacaacac ggtgcgccaa cccttacatt 6000

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ttcagctccg ccatcgccgc ttccactttt tcccgcgttt tcgcagaaac gtggctggcc 6060

6001 aagtcgaggc ggtagcggcg aaggtgaaaa agggcgcaaa agcgtctttg caccgaccgg 6060

ORF 3 .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 tggttcacca cgcgggaaac ggtctgataa gagacaccgg catactctgc gacatcgtat 6120

6061 accaagtggt gcgccctttg ccagactatt ctctgtggcc gtatgagacg ctgtagcata 6120

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 aacgttactg gtttcacatt caccaccctg aattgactct cttccgggcg ctatcatgcc 6180

6121 ttgcaatgac caaagtgtaa gtggtgggac ttaactgaga gaaggcccgc gatagtacgg 6180

.......... lacI .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ataccgcgaa aggttttgcg ccattcgatg gtgtccggga tctcgacgct ctcccttatg 6240

6181 tatggcgctt tccaaaacgc ggtaagctac cacaggccct agagctgcga gagggaatac 6240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 cgactcctgc attaggaagc agcccagtag taggttgagg ccgttgagca ccgccgccgc 6300

6241 gctgaggacg taatccttcg tcgggtcatc atccaactcc ggcaactcgt ggcggcggcg 6300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 aaggaatggt gcatg..... .......... .......... .......... .......... 6315

6301 ttccttacca cgtac..... .......... .......... .......... .......... 6315

.......... .......... .......... .......... .......... ..........