Sequence of pET104.1

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Sequence of pET104.1_DEST

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Sequence of pET104.1 DEST : 7650 BP; 1866 A; 1922 C; 2051 G; 1811 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa 60

1 gttcctctac cgcgggttgt cagggggccg gtgccccgga cggtggtatg ggtgcggctt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat 120

61 tgttcgcgag tactcgggct tcaccgctcg ggctagaagg ggtagccact acagccgcta 120

.......... .......... .......... .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta 180

121 tatccgcggt cgttggcgtg gacaccgcgg ccactacggc cggtgctacg caggccgcat 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg 240

181 ctcctagctc tagagctagg gcgctttaat tatgctgagt gatatcccct taacactcgc 240

.......... .......... .......... T7_pro .......... lacO

.......... XbaI(255) .......... .......... .......... NdeI(295)

241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat atacatatgg 300

241 ctattgttaa ggggagatct ttattaaaac aaattgaaat tcttcctcta tatgtatacc 300

lacO .......... ....... T7_transl_en_RBS ....... ..........

.......... .......... .......... .......... .......... ..........

301 ctagcatgac tggtggacag caaatgggta ttatgggcgc cggcaccccg gtgaccgccc 360

301 gatcgtactg accacctgtc gtttacccat aatacccgcg gccgtggggc cactggcggg 360

Xpress_fwd_pri T7_gene10_leader T7_leader .......... ..........

.......... .......... .......... .......... .......... ..........

361 cgctggcggg cactatctgg aaggtgctgg ccagcgaagg ccagacggtg gccgcaggcg 420

361 gcgaccgccc gtgatagacc ttccacgacc ggtcgcttcc ggtctgccac cggcgtccgc 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aggtgctgct gattctggaa gccatgaaga tggaaaccga aatccgcgcc gcgcaggccg 480

421 tccacgacga ctaagacctt cggtacttct acctttggct ttaggcgcgg cgcgtccggc 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggaccgtgcg cggtatcgcg gtgaaagccg gcgacgcggt ggcggtcggc gacaccctga 540

481 cctggcacgc gccatagcgc cactttcggc cgctgcgcca ccgccagccg ctgtgggact 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgaccctggc gggctctgga tccgatctgt acgacgatga cgataaggga attatcacaa 600

541 actgggaccg cccgagacct aggctagaca tgctgctact gctattccct taatagtgtt 600

.......... .......... .......... Xpress_EK .......... ..........

.......... .......... .......... .......... .......... ..........

601 gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat aaatatcaat atattaaatt 660

601 caaacatgtt ttttcgactt gctctttgca ttttactata tttatagtta tataatttaa 660

.......... .......... attR1 attR2 .......... ..........

.......... .......... .......... .......... .......... ..........

661 agattttgca taaaaaacag actacataat actgtaaaac acaacatatc cagtcactat 720

661 tctaaaacgt attttttgtc tgatgtatta tgacattttg tgttgtatag gtcagtgata 720

.......... ......... attR1 attR2 ......... .......... ..........

NotI(723) .......... .......... .......... .......... ..........

721 ggcggccgca ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg 780

721 ccgccggcgt aatccgtggg gtccgaaatg tgaaatacga aggccgagca tattacacac 780

.......... .......... .......... lac_pro .......... ..........

.......... .......... .......... .......... .......... ..........

781 gattttgagt taggatccgt cgagattttc aggagctaag gaagctaaaa tggagaaaaa 840

781 ctaaaactca atcctaggca gctctaaaag tcctcgattc cttcgatttt acctcttttt 840

.......... .......... .......... .......... ........ ORF 2 CAT/CamR

.......... .......... .......... .......... .......... ..........

841 aatcactgga tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc 900

841 ttagtgacct atatggtggc aactatatag ggttaccgta gcatttcttg taaaactccg 900

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 atttcagtca gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt 960

901 taaagtcagt caacgagtta catggatatt ggtctggcaa gtcgacctat aatgccggaa 960

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 tttaaagacc gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc 1020

961 aaatttctgg catttctttt tattcgtgtt caaaataggc cggaaataag tgtaagaacg 1020

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... ......... EcoRI(1043) ......... .......... ..........

1021 ccgcctgatg aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat 1080

1021 ggcggactac ttacgagtag gccttaaggc ataccgttac tttctgccac tcgaccacta 1080

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atgggatagt gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc 1140

1081 taccctatca caagtgggaa caatgtggca aaaggtactc gtttgacttt gcaaaagtag 1140

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gctctggagt gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt 1200

1141 cgagacctca cttatggtgc tgctaaaggc cgtcaaagat gtgtatataa gcgttctaca 1200

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggcgtgttac ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt 1260

1201 ccgcacaatg ccacttttgg accggataaa gggatttccc aaataactct tatacaaaaa 1260

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cgtctcagcc aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga 1320

1261 gcagagtcgg ttagggaccc actcaaagtg gtcaaaacta aatttgcacc ggttatacct 1320

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... NcoI(1344) .......... .......... ..........

1321 caacttcttc gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct 1380

1321 gttgaagaag cgggggcaaa agtggtaccc gtttataata tgcgttccgc tgttccacga 1380

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gatgccgctg gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat 1440

1381 ctacggcgac cgctaagtcc aagtagtacg gcagacacta ccgaaggtac agccgtctta 1440

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gcttaatgaa ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc 1500

1441 cgaattactt aatgttgtca tgacgctact caccgtcccg ccccgcattt gcgcacctag 1500

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... AgeI(1543) ..........

1501 cggcttacta aaagccagat aacagtatgc gtatttgcgc gcaccggtgc tagcgtatac 1560

1501 gccgaatgat tttcggtcta ttgtcatacg cataaacgcg cgtggccacg atcgcatatg 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ccgaagtatg tcaaaaagag gtgtgctatg aagcagcgta ttacagtgac agttgacagc 1620

1561 ggcttcatac agtttttctc cacacgatac ttcgtcgcat aatgtcactg tcaactgtcg 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gacagctatc agttgctcaa ggcatatatg atgtcaatat ctccggtctg gtaagcacaa 1680

1621 ctgtcgatag tcaacgagtt ccgtatatac tacagttata gaggccagac cattcgtgtt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccatgcagaa tgaagcccgt cgtctgcgtg ccgaacgctg gaaagcggaa aatcaggaag 1740

1681 ggtacgtctt acttcgggca gcagacgcac ggcttgcgac ctttcgcctt ttagtccttc 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggatggctga ggtcgcccgg tttattgaaa tgaacggctc ttttgctgac gagaacaggg 1800

1741 cctaccgact ccagcgggcc aaataacttt acttgccgag aaaacgactg ctcttgtccc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 actggtgaaa tgcagtttaa ggtttacacc tataaaagag agagccgtta tcgtctgttt 1860

1801 tgaccacttt acgtcaaatt ccaaatgtgg atattttctc tctcggcaat agcagacaaa 1860

.......... .......... .......... ccdB .......... ..........

.......... .......... .......... SmaI(1893) .......... ..........

1861 gtggatgtac agagtgatat tattgacacg cccgggcgac ggatggtgat ccccctggcc 1920

1861 cacctacatg tctcactata ataactgtgc gggcccgctg cctaccacta gggggaccgg 1920

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agtgcacgtc tgctgtcaga taaagtctcc cgtgaacttt acccggtggt gcatatcggg 1980

1921 tcacgtgcag acgacagtct atttcagagg gcacttgaaa tgggccacca cgtatagccc 1980

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gatgaaagct ggcgcatgat gaccaccgat atggccagtg tgccggtctc cgttatcggg 2040

1981 ctactttcga ccgcgtacta ctggtggcta taccggtcac acggccagag gcaatagccc 2040

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gaagaagtgg ctgatctcag ccaccgcgaa aatgacatca aaaacgccat taacctgatg 2100

2041 cttcttcacc gactagagtc ggtggcgctt ttactgtagt ttttgcggta attggactac 2100

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... SalI(2150) ..........

2101 ttctggggaa tataaatgtc aggctccgtt atacacagcc agtctgcagg tcgaccatag 2160

2101 aagacccctt atatttacag tccgaggcaa tatgtgtcgg tcagacgtcc agctggtatc 2160

ccdB .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgactggata tgttgtgttt tacagtatta tgtagtctgt tttttatgca aaatctaatt 2220

2161 actgacctat acaacacaaa atgtcataat acatcagaca aaaaatacgt tttagattaa 2220

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 taatatattg atatttatat cattttacgt ttctcgttca gctttcttgt acaaagtggt 2280

2221 attatataac tataaatata gtaaaatgca aagagcaagt cgaaagaaca tgtttcacca 2280

.......... attR2 attR1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gataattaat taagatcaga tccggctgct aacaaagccc gaaaggaagc tgagttggct 2340

2281 ctattaatta attctagtct aggccgacga ttgtttcggg ctttccttcg actcaaccga 2340

.......... .......... .......... .......... T7_term ..........

.......... .......... .......... .......... .......... ..........

2341 gctgccaccg ctgagcaata actagcataa ccccttgggg cctctaaacg ggtcttgagg 2400

2341 cgacggtggc gactcgttat tgatcgtatt ggggaacccc ggagatttgc ccagaactcc 2400

....... T7_Terminal_pri T7_term ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggttttttgc tgaaaggagg aactatatcc ggatatcccg caagaggccc ggcagtaccg 2460

2401 ccaaaaaacg actttcctcc ttgatatagg cctatagggc gttctccggg ccgtcatggc 2460

.......... T7_term .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcataaccaa gcctatgcct acagcatcca gggtgacggt gccgaggatg acgatgagcg 2520

2461 cgtattggtt cggatacgga tgtcgtaggt cccactgcca cggctcctac tgctactcgc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cattgttaga tttcatacac ggtgcctgac tgcgttagca atttaactgt gataaactac 2580

2521 gtaacaatct aaagtatgtg ccacggactg acgcaatcgt taaattgaca ctatttgatg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cgcattaaag ctagcttatc gatgataagc tgtcaaacat gagaattaat tcttgaagac 2640

2581 gcgtaatttc gatcgaatag ctactattcg acagtttgta ctcttaatta agaacttctg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gaaagggcct cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt 2700

2641 ctttcccgga gcactatgcg gataaaaata tccaattaca gtactattat taccaaagaa 2700

.......... .......... .......... .......... .......... ..........

AatII(2706) ........ .......... .......... .......... ..........

2701 agacgtcagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct 2760

2701 tctgcagtcc accgtgaaaa gcccctttac acgcgccttg gggataaaca aataaaaaga 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat 2820

2761 tttatgtaag tttatacata ggcgagtact ctgttattgg gactatttac gaagttatta 2820

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg 2880

2821 taactttttc cttctcatac tcataagttg taaaggcaca gcgggaataa gggaaaaaac 2880

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2881 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg 2940

2881 gccgtaaaac ggaaggacaa aaacgagtgg gtctttgcga ccactttcat tttctacgac 2940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc 3000

2941 ttctagtcaa cccacgtgct cacccaatgt agcttgacct agagttgtcg ccattctagg 3000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat 3060

3001 aactctcaaa agcggggctt cttgcaaaag gttactactc gtgaaaattt caagacgata 3060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtggcgcggt attatcccgt gttgacgccg ggcaagagca actcggtcgc cgcatacact 3120

3061 caccgcgcca taatagggca caactgcggc ccgttctcgt tgagccagcg gcgtatgtga 3120

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca 3180

3121 taagagtctt actgaaccaa ctcatgagtg gtcagtgtct tttcgtagaa tgcctaccgt 3180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact 3240

3181 actgtcattc tcttaatacg tcacgacggt attggtactc actattgtga cgccggttga 3240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg 3300

3241 atgaagactg ttgctagcct cctggcttcc tcgattggcg aaaaaacgtg ttgtaccccc 3300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg 3360

3301 tagtacattg agcggaacta gcaacccttg gcctcgactt acttcggtat ggtttgctgc 3360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 agcgtgacac cacgatgcct gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg 3420

3361 tcgcactgtg gtgctacgga cgtcgttacc gttgttgcaa cgcgtttgat aattgaccgc 3420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg 3480

3421 ttgatgaatg agatcgaagg gccgttgtta attatctgac ctacctccgc ctatttcaac 3480

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag 3540

3481 gtcctggtga agacgcgagc cgggaaggcc gaccgaccaa ataacgacta tttagacctc 3540

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc 3600

3541 ggccactcgc acccagagcg ccatagtaac gtcgtgaccc cggtctacca ttcgggaggg 3600

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga 3660

3601 catagcatca atagatgtgc tgcccctcag tccgttgata cctacttgct ttatctgtct 3660

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat 3720

3661 agcgactcta tccacggagt gactaattcg taaccattga cagtctggtt caaatgagta 3720

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 3780

3721 tatatgaaat ctaactaaat tttgaagtaa aaattaaatt ttcctagatc cacttctagg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 3840

3781 aaaaactatt agagtactgg ttttagggaa ttgcactcaa aagcaaggtg actcgcagtc 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 3900

3841 tggggcatct tttctagttt cctagaagaa ctctaggaaa aaaagacgcg cattagacga 3900

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3901 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 3960

3901 cgaacgtttg tttttttggt ggcgatggtc gccaccaaac aaacggccta gttctcgatg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 4020

3961 gttgagaaaa aggcttccat tgaccgaagt cgtctcgcgt ctatggttta tgacaggaag 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 4080

4021 atcacatcgg catcaatccg gtggtgaagt tcttgagaca tcgtggcgga tgtatggagc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 4140

4081 gagacgatta ggacaatggt caccgacgac ggtcaccgct attcagcaca gaatggccca 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 4200

4141 acctgagttc tgctatcaat ggcctattcc gcgtcgccag cccgacttgc cccccaagca 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 4260

4201 cgtgtgtcgg gtcgaacctc gcttgctgga tgtggcttga ctctatggat gtcgcactcg 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 4320

4261 atactctttc gcggtgcgaa gggcttccct ctttccgcct gtccataggc cattcgccgt 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 4380

4321 cccagccttg tcctctcgcg tgctccctcg aaggtccccc tttgcggacc atagaaatat 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 4440

4381 caggacagcc caaagcggtg gagactgaac tcgcagctaa aaacactacg agcagtcccc 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 4500

4441 ccgcctcgga tacctttttg cggtcgttgc gccggaaaaa tgccaaggac cggaaaacga 4500

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 4560

4501 ccggaaaacg agtgtacaag aaaggacgca ataggggact aagacaccta ttggcataat 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag 4620

4561 ggcggaaact cactcgacta tggcgagcgg cgtcggcttg ctggctcgcg tcgctcagtc 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tgagcgagga agcggaagag cgcctgatgc ggtattttct ccttacgcat ctgtgcggta 4680

4621 actcgctcct tcgccttctc gcggactacg ccataaaaga ggaatgcgta gacacgccat 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tttcacaccg catatatggt gcactctcag tacaatctgc tctgatgccg catagttaag 4740

4681 aaagtgtggc gtatatacca cgtgagagtc atgttagacg agactacggc gtatcaattc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ccagtataca ctccgctatc gctacgtgac tgggtcatgg ctgcgccccg acacccgcca 4800

4741 ggtcatatgt gaggcgatag cgatgcactg acccagtacc gacgcggggc tgtgggcggt 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 acacccgctg acgcgccctg acgggcttgt ctgctcccgg catccgctta cagacaagct 4860

4801 tgtgggcgac tgcgcgggac tgcccgaaca gacgagggcc gtaggcgaat gtctgttcga 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gtgaccgtct ccgggagctg catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg 4920

4861 cactggcaga ggccctcgac gtacacagtc tccaaaagtg gcagtagtgg ctttgcgcgc 4920

.......... .......... pGEX_3_pri .......... ROP ..........

.......... .......... .......... .......... .......... ..........

4921 aggcagctgc ggtaaagctc atcagcgtgg tcgtgaagcg attcacagat gtctgcctgt 4980

4921 tccgtcgacg ccatttcgag tagtcgcacc agcacttcgc taagtgtcta cagacggaca 4980

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tcatccgcgt ccagctcgtt gagtttctcc agaagcgtta atgtctggct tctgataaag 5040

4981 agtaggcgca ggtcgagcaa ctcaaagagg tcttcgcaat tacagaccga agactatttc 5040

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgggccatgt taagggcggt tttttcctgt ttggtcactg atgcctccgt gtaaggggga 5100

5041 gcccggtaca attcccgcca aaaaaggaca aaccagtgac tacggaggca cattccccct 5100

.......... ROP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tttctgttca tgggggtaat gataccgatg aaacgagaga ggatgctcac gatacgggtt 5160

5101 aaagacaagt acccccatta ctatggctac tttgctctct cctacgagtg ctatgcccaa 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 actgatgatg aacatgcccg gttactggaa cgttgtgagg gtaaacaact ggcggtatgg 5220

5161 tgactactac ttgtacgggc caatgacctt gcaacactcc catttgttga ccgccatacc 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 atgcggcggg accagagaaa aatcactcag ggtcaatgcc agcgcttcgt taatacagat 5280

5221 tacgccgccc tggtctcttt ttagtgagtc ccagttacgg tcgcgaagca attatgtcta 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gtaggtgttc cacagggtag ccagcagcat cctgcgatgc agatccggaa cataatggtg 5340

5281 catccacaag gtgtcccatc ggtcgtcgta ggacgctacg tctaggcctt gtattaccac 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cagggcgctg acttccgcgt ttccagactt tacgaaacac ggaaaccgaa gaccattcat 5400

5341 gtcccgcgac tgaaggcgca aaggtctgaa atgctttgtg cctttggctt ctggtaagta 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gttgttgctc aggtcgcaga cgttttgcag cagcagtcgc ttcacgttcg ctcgcgtatc 5460

5401 caacaacgag tccagcgtct gcaaaacgtc gtcgtcagcg aagtgcaagc gagcgcatag 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 ggtgattcat tctgctaacc agtaaggcaa ccccgccagc ctagccgggt cctcaacgac 5520

5461 ccactaagta agacgattgg tcattccgtt ggggcggtcg gatcggccca ggagttgctg 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aggagcacga tcatgcgcac ccgtggccag gacccaacgc tgcccgagat gcgccgcgtg 5580

5521 tcctcgtgct agtacgcgtg ggcaccggtc ctgggttgcg acgggctcta cgcggcgcac 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cggctgctgg agatggcgga cgcgatggat atgttctgcc aagggttggt ttgcgcattc 5640

5581 gccgacgacc tctaccgcct gcgctaccta tacaagacgg ttcccaacca aacgcgtaag 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 acagttctcc gcaagaattg attggctcca attcttggag tggtgaatcc gttagcgagg 5700

5641 tgtcaagagg cgttcttaac taaccgaggt taagaacctc accacttagg caatcgctcc 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 tgccgccggc ttccattcag gtcgaggtgg cccggctcca tgcaccgcga cgcaacgcgg 5760

5701 acggcggccg aaggtaagtc cagctccacc gggccgaggt acgtggcgct gcgttgcgcc 5760

.......... .......... ....... tet(0-612) ORF 2 ....... ..........

.......... .......... .......... .......... .......... ..........

5761 ggaggcagac aaggtatagg gcggcgccta caatccatgc caacccgttc catgtgctcg 5820

5761 cctccgtctg ttccatatcc cgccgcggat gttaggtacg gttgggcaag gtacacgagc 5820

.......... ....... tet(0-612) ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ccgaggcggc ataaatcgcc gtgacgatca gcggtccagt gatcgaagtt aggctggtaa 5880

5821 ggctccgccg tatttagcgg cactgctagt cgccaggtca ctagcttcaa tccgaccatt 5880

.......... ....... tet(0-612) ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gagccgcgag cgatccttga agctgtccct gatggtcgtc atctacctgc c tggacagca 5940

5881 ctcggcgctc gctaggaact tcgacaggga ctaccagcag tagatggacg gacctgtcgt 5940

.......... ....... tet(0-612) ORF 2 ....... ORF 2 ..........

.......... .......... .......... .......... .......... ..........

5941 tggcctgcaa cgcgggcatc ccgatgccgc cggaagcgag aagaatcata atggggaagg 6000

5941 accggacgtt gcgcccgtag ggctacggcg gccttcgctc ttcttagtat taccccttcc 6000

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... NruI(6018) .......... .......... .......... ..........

6001 ccatccagcc tcgcgtcgcg aacgccagca agacgtagcc cagcgcgtcg gccgccatgc 6060

6001 ggtaggtcgg agcgcagcgc ttgcggtcgt tctgcatcgg gtcgcgcagc cggcggtacg 6060

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 cggcgataat ggcctgcttc tcgccgaaac gtttggtggc gggaccagtg acgaaggctt 6120

6061 gccgctatta ccggacgaag agcggctttg caaaccaccg ccctggtcac tgcttccgaa 6120

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gagcgagggc gtgcaagatt ccgaataccg caagcgacag gccgatcatc gtcgcgctcc 6180

6121 ctcgctcccg cacgttctaa ggcttatggc gttcgctgtc cggctagtag cagcgcgagg 6180

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 agcgaaagcg gtcctcgccg aaaatgaccc agagcgctgc cggcacctgt cctacgagtt 6240

6181 tcgctttcgc caggagcggc ttttactggg tctcgcgacg gccgtggaca ggatgctcaa 6240

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gcatgataaa gaagacagtc ataagtgcgg cgacgatagt catgccccgc gcccaccgga 6300

6241 cgtactattt cttctgtcag tattcacgcc gctgctatca gtacggggcg cgggtggcct 6300

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 aggagctgac tgggttgaag gctctcaagg gcatcggtcg agatcccggt gcctaatgag 6360

6301 tcctcgactg acccaacttc cgagagttcc cgtagccagc tctagggcca cggattactc 6360

....... tet(0-612) ORF 2 ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 tgagctaact tacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt 6420

6361 actcgattga atgtaattaa cgcaacgcga gtgacgggcg aaaggtcagc cctttggaca 6420

.......... .......... ORF 2 .......... ORF 3 lacI ..........

.......... .......... .......... .......... .......... ..........

6421 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc 6480

6421 gcacggtcga cgtaattact tagccggttg cgcgcccctc tccgccaaac gcataacccg 6480

.......... ....... ORF 2 ORF 3 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gccagggtgg tttttctttt caccagtgag acgggcaaca gctgattgcc cttcaccgcc 6540

6481 cggtcccacc aaaaagaaaa gtggtcactc tgcccgttgt cgactaacgg gaagtggcgg 6540

.......... ....... ORF 2 ORF 3 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 tggccctgag agagttgcag caagcggtcc acgctggttt gccccagcag gcgaaaatcc 6600

6541 accgggactc tctcaacgtc gttcgccagg tgcgaccaaa cggggtcgtc cgcttttagg 6600

.......... ....... ORF 2 ORF 3 lacI ....... .......... ..........

.......... HpaI(6615) .......... .......... .......... ..........

6601 tgtttgatgg tggttaacgg cgggatataa catgagctgt cttcggtatc gtcgtatccc 6660

6601 acaaactacc accaattgcc gccctatatt gtactcgaca gaagccatag cagcataggg 6660

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 actaccgaga tatccgcacc aacgcgcagc ccggactcgg taatggcgcg cattgcgccc 6720

6661 tgatggctct ataggcgtgg ttgcgcgtcg ggcctgagcc attaccgcgc gtaacgcggg 6720

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 agcgccatct gatcgttggc aaccagcatc gcagtgggaa cgatgccctc attcagcatt 6780

6721 tcgcggtaga ctagcaaccg ttggtcgtag cgtcaccctt gctacgggag taagtcgtaa 6780

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 tgcatggttt gttgaaaacc ggacatggca ctccagtcgc cttcccgttc cgctatcggc 6840

6781 acgtaccaaa caacttttgg cctgtaccgt gaggtcagcg gaagggcaag gcgatagccg 6840

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 tgaatttgat tgcgagtgag atatttatgc cagccagcca gacgcagacg cgccgagaca 6900

6841 acttaaacta acgctcactc tataaatacg gtcggtcggt ctgcgtctgc gcggctctgt 6900

.......... .......... ORF 3 lacI .......... .......... ..........

.......... ApaI(6914) .......... .......... .......... ..........

6901 gaacttaatg ggcccgctaa cagcgcgatt tgctggtgac ccaatgcgac cagatgctcc 6960

6901 cttgaattac ccgggcgatt gtcgcgctaa acgaccactg ggttacgctg gtctacgagg 6960

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 acgcccagtc gcgtaccgtc ttcatgggag aaaataatac tgttgatggg tgtctggtca 7020

6961 tgcgggtcag cgcatggcag aagtaccctc ttttattatg acaactaccc acagaccagt 7020

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 gagacatcaa gaaataacgc cggaacatta gtgcaggcag cttccacagc aatggcatcc 7080

7021 ctctgtagtt ctttattgcg gccttgtaat cacgtccgtc gaaggtgtcg ttaccgtagg 7080

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... BclI(7103) .......... .......... ..........

7081 tggtcatcca gcggatagtt aatgatcagc ccactgacgc gttgcgcgag aagattgtgc 7140

7081 accagtaggt cgcctatcaa ttactagtcg ggtgactgcg caacgcgctc ttctaacacg 7140

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 accgccgctt tacaggcttc gacgccgctt cgttctacca tcgacaccac cacgctggca 7200

7141 tggcggcgaa atgtccgaag ctgcggcgaa gcaagatggt agctgtggtg gtgcgaccgt 7200

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 cccagttgat cggcgcgaga tttaatcgcc gcgacaattt gcgacggcgc gtgcagggcc 7260

7201 gggtcaacta gccgcgctct aaattagcgg cgctgttaaa cgctgccgcg cacgtcccgg 7260

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 agactggagg tggcaacgcc aatcagcaac gactgtttgc ccgccagttg ttgtgccacg 7320

7261 tctgacctcc accgttgcgg ttagtcgttg ctgacaaacg ggcggtcaac aacacggtgc 7320

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 cggttgggaa tgtaattcag ctccgccatc gccgcttcca ctttttcccg cgttttcgca 7380

7321 gccaaccctt acattaagtc gaggcggtag cggcgaaggt gaaaaagggc gcaaaagcgt 7380

.......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 gaaacgtggc tggcctggtt caccacgcgg gaaacggtct gataagagac accggcatac 7440

7381 ctttgcaccg accggaccaa gtggtgcgcc ctttgccaga ctattctctg tggccgtatg 7440

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 tctgcgacat cgtataacgt tactggtttc acattcacca ccctgaattg actctcttcc 7500

7441 agacgctgta gcatattgca atgaccaaag tgtaagtggt gggacttaac tgagagaagg 7500

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 gggcgctatc atgccatacc gcgaaaggtt ttgcgccatt cgatggtgtc cgggatctcg 7560

7501 cccgcgatag tacggtatgg cgctttccaa aacgcggtaa gctaccacag gccctagagc 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 acgctctccc ttatgcgact cctgcattag gaagcagccc agtagtaggt tgaggccgtt 7620

7561 tgcgagaggg aatacgctga ggacgtaatc cttcgtcggg tcatcatcca actccggcaa 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 gagcaccgcc gccgcaagga atggtgcatg .......... .......... .......... 7650

7621 ctcgtggcgg cggcgttcct taccacgtac .......... .......... .......... 7650

.......... .......... .......... .......... .......... ..........