Sequence of pET11a

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Sequence of pET11a

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Sequence of pET11a : 5677 BP; 1299 A; 1550 C; 1496 G; 1332 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 240

181 cgccctatag gcctatatca aggaggaaag tcgttttttg gggagttctg ggcaaatctc 240

.......... .......... .......... T7_term .......... ..........

.......... .......... .......... .......... .......... ..........

241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 300

241 cggggttccc caatacgatc aataacgagt cgccaccgtc gtcggttgag tcgaaggaaa 300

.......... ... T7_term T7_Terminal_pri ... .......... ..........

.......... BamHI(319) .......... .......... .......... NheI(352)

301 cgggctttgt tagcagccgg atccgcgacc catttgctgt ccaccagtca tgctagccat 360

301 gcccgaaaca atcgtcggcc taggcgctgg gtaaacgaca ggtggtcagt acgatcggta 360

T7_term .......... .......... T7_gene10_leader T7_leader Xpress_fwd_pri

.......... .......... .......... .......... .......... ..........

361 atgtatatct ccttcttaaa gttaaacaaa attatttcta gaggggaatt gttatccgct 420

361 tacatataga ggaagaattt caatttgttt taataaagat ctccccttaa caataggcga 420

....... T7_transl_en_RBS ....... .......... .......... lacO

.......... .......... .......... .......... BglII(463) ..........

421 cacaattccc ctatagtgag tcgtattaat ttcgcgggat cgagatctcg atcctctacg 480

421 gtgttaaggg gatatcactc agcataatta aagcgcccta gctctagagc taggagatgc 480

lacO T7_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ccggacgcat cgtggccggc atcaccggcg ccacaggtgc ggttgctggc gcctatatcg 540

481 ggcctgcgta gcaccggccg tagtggccgc ggtgtccacg ccaacgaccg cggatatagc 540

.......... .......... .......... .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

541 ccgacatcac cgatggggaa gatcgggctc gccacttcgg gctcatgagc gcttgtttcg 600

541 ggctgtagtg gctacccctt ctagcccgag cggtgaagcc cgagtactcg cgaacaaagc 600

pBRrevBam_pri ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt gggcgccatc tccttgcatg 660

601 cgcacccata ccaccgtccg gggcaccggc cccctgacaa cccgcggtag aggaacgtac 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 caccattcct tgcggcggcg gtgctcaacg gcctcaacct actactgggc tgcttcctaa 720

661 gtggtaagga acgccgccgc cacgagttgc cggagttgga tgatgacccg acgaaggatt 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgcaggagtc gcataaggga gagcgtcgag atcccggaca ccatcgaatg gcgcaaaacc 780

721 acgtcctcag cgtattccct ctcgcagctc tagggcctgt ggtagcttac cgcgttttgg 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa 840

781 aaagcgccat accgtactat cgcgggcctt ctctcagtta agtcccacca cttacacttt 840

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

841 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc 900

841 ggtcattgca atatgctaca gcgtctcata cggccacaga gaatagtctg gcaaagggcg 900

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg 960

901 caccacttgg tccggtcggt gcaaagacgc ttttgcgccc tttttcacct tcgccgctac 960

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg 1020

961 cgcctcgact taatgtaagg gttggcgcac cgtgttgttg accgcccgtt tgtcagcaac 1020

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg 1080

1021 gactaaccgc aacggtggag gtcagaccgg gacgtgcgcg gcagcgttta acagcgccgc 1080

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc 1140

1081 taatttagag cgcggctagt tgacccacgg tcgcaccacc acagctacca tcttgcttcg 1140

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1199)

1141 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg 1200

1141 ccgcagcttc ggacatttcg ccgccacgtg ttagaagagc gcgttgcgca gtcacccgac 1200

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat 1260

1201 tagtaattga taggcgacct actggtccta cggtaacgac accttcgacg gacgtgatta 1260

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc 1320

1261 caaggccgca ataaagaact acagagactg gtctgtgggt agttgtcata ataaaagagg 1320

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc 1380

1321 gtacttctgc catgcgctga cccgcacctc gtagaccagc gtaacccagt ggtcgtttag 1380

.......... .......... lacI ORF 1 .......... .......... ..........

.......... ApaI(1396) .......... .......... .......... ..........

1381 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat 1440

1381 cgcgacaatc gcccgggtaa ttcaagacag agccgcgcag acgcagaccg accgaccgta 1440

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc 1500

1441 tttatagagt gagcgttagt ttaagtcggc tatcgccttg cccttccgct gacctcacgg 1500

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg 1560

1501 tacaggccaa aagttgtttg gtacgtttac gacttactcc cgtagcaagg gtgacgctac 1560

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg 1620

1561 gaccaacggt tgctagtcta ccgcgacccg cgttacgcgc ggtaatggct caggcccgac 1620

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat 1680

1621 gcgcaaccac gcctatagag ccatcaccct atgctgctat ggcttctgtc gagtacaata 1680

.......... .......... lacI ORF 1 .......... .......... ..........

.......... HpaI(1691) .......... .......... .......... ..........

1681 atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac 1740

1681 tagggcggca attggtggta gtttgtccta aaagcggacg accccgtttg gtcgcacctg 1740

.......... .......... lacI ORF 1 ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca 1800

1741 gcgaacgacg ttgagagagt cccggtccgc cacttcccgt tagtcgacaa cgggcagagt 1800

.......... ....... lacI ORF 1 ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg 1860

1801 gaccactttt ctttttggtg ggaccgcggg ttatgcgttt ggcggagagg ggcgcgcaac 1860

.......... ....... lacI ORF 1 ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg 1920

1861 cggctaagta attacgtcga ccgtgctgtc caaagggctg acctttcgcc cgtcactcgc 1920

.......... ....... lacI ORF 1 ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 caacgcaatt aatgtaagtt agctcactca ttaggcaccg ggatctcgac cgatgccctt 1980

1921 gttgcgttaa ttacattcaa tcgagtgagt aatccgtggc cctagagctg gctacgggaa 1980

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc 2040

1981 ctctcggaag ttgggtcagt cgaggaaggc cacccgcgcc ccgtactgat agcagcggcg 2040

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2041 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt 2100

2041 tgaatactga cagaagaaat agtacgttga gcatcctgtc cacggccgtc gcgagaccca 2100

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt 2160

2101 gtaaaagccg ctcctggcga aagcgacctc gcgctgctac tagccggaca gcgaacgcca 2160

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2161 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt 2220

2161 taagccttag aacgtgcggg agcgagttcg gaagcagtga ccagggcggt ggtttgcaaa 2220

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct 2280

2221 gccgctcttc gtccggtaat agcggccgta ccgccggctg cgcgacccga tgcagaacga 2280

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

NruI(2288) .......... .......... .......... .......... ..........

2281 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg 2340

2281 ccgcaagcgc tgcgctccga cctaccggaa ggggtaatac taagaagagc gaaggccgcc 2340

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2341 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg 2400

2341 gtagccctac gggcgcaacg tccggtacga caggtccgtc catctactgc tggtagtccc 2400

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcactg gaccgctgat 2460

2401 tgtcgaagtt cctagcgagc gccgagaatg gtcggattga agctagtgac ctggcgacta 2460

.......... ..... ORF 2 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg 2520

2461 gcagtgccgc taaatacggc ggagccgctc gtgtaccttg cccaaccgta cctaacatcc 2520

.......... ..... ORF 2 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac 2580

2521 gcggcgggat atggaacaga cggaggggcg caacgcagcg ccacgtacct cggcccggtg 2580

.......... ..... ORF 2 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga 2640

2581 gagctggact taccttcggc cgccgtggag cgattgccta agtggtgagg ttcttaacct 2640

ORF 2 tet(1191-576) .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttggca gaacatatcc 2700

2641 cggttagtta agaacgcctc ttgacactta cgcgtttggt tgggaaccgt cttgtatagg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... MscI(2760)

2701 atcgcgtccg ccatctccag cagccgcacg cggcgcatct cgggcagcgt tgggtcctgg 2760

2701 tagcgcaggc ggtagaggtc gtcggcgtgc gccgcgtaga gcccgtcgca acccaggacc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ccacgggtgc gcatgatcgt gctcctgtcg ttgaggaccc ggctaggctg gcggggttgc 2820

2761 ggtgcccacg cgtactagca cgaggacagc aactcctggg ccgatccgac cgccccaacg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cttactggtt agcagaatga atcaccgata cgcgagcgaa cgtgaagcga ctgctgctgc 2880

2821 gaatgaccaa tcgtcttact tagtggctat gcgctcgctt gcacttcgct gacgacgacg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aaaacgtctg cgacctgagc aacaacatga atggtcttcg gtttccgtgt ttcgtaaagt 2940

2881 ttttgcagac gctggactcg ttgttgtact taccagaagc caaaggcaca aagcatttca 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctggaaacgc ggaagtcagc gccctgcacc attatgttcc ggatctgcat cgcaggatgc 3000

2941 gacctttgcg ccttcagtcg cgggacgtgg taatacaagg cctagacgta gcgtcctacg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgctggctac cctgtggaac acctacatct gtattaacga agcgctggca ttgaccctga 3060

3001 acgaccgatg ggacaccttg tggatgtaga cataattgct tcgcgaccgt aactgggact 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gtgatttttc tctggtcccg ccgcatccat accgccagtt gtttaccctc acaacgttcc 3120

3061 cactaaaaag agaccagggc ggcgtaggta tggcggtcaa caaatgggag tgttgcaagg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agtaaccggg catgttcatc atcagtaacc cgtatcgtga gcatcctctc tcgtttcatc 3180

3121 tcattggccc gtacaagtag tagtcattgg gcatagcact cgtaggagag agcaaagtag 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggtatcatta cccccatgaa cagaaatccc ccttacacgg aggcatcagt gaccaaacag 3240

3181 ccatagtaat gggggtactt gtctttaggg ggaatgtgcc tccgtagtca ctggtttgtc 3240

.......... .......... .......... .......... .......... ROP

.......... .......... .......... .......... .......... ..........

3241 gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac gcttctggag 3300

3241 cttttttggc gggaattgta ccgggcgaaa tagtcttcgg tctgtaattg cgaagacctc 3300

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct tcacgaccac 3360

3301 tttgagttgc tcgacctgcg cctacttgtc cgtctgtaga cacttagcga agtgctggtg 3360

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga aaacctctga 3420

3361 cgactactcg aaatggcgtc gacggagcgc gcaaagccac tactgccact tttggagact 3420

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 3480

3421 gtgtacgtcg agggcctctg ccagtgtcga acagacattc gcctacggcc ctcgtctgtt 3480

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca 3540

3481 cgggcagtcc cgcgcagtcg cccacaaccg cccacagccc cgcgtcggta ctgggtcagt 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga 3600

3541 gcatcgctat cgcctcacat atgaccgaat tgatacgccg tagtctcgtc taacatgact 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gagtgcacca tatatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat 3660

3601 ctcacgtggt atatacgcca cactttatgg cgtgtctacg cattcctctt ttatggcgta 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 caggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg 3720

3661 gtccgcgaga aggcgaagga gcgagtgact gagcgacgcg agccagcaag ccgacgccgc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc 3780

3721 tcgccatagt cgagtgagtt tccgccatta tgccaatagg tgtcttagtc ccctattgcg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt 3840

3781 tcctttcttg tacactcgtt ttccggtcgt tttccggtcc ttggcatttt tccggcgcaa 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gctggcgttt ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag 3900

3841 cgaccgcaaa aaggtatccg aggcgggggg actgctcgta gtgtttttag ctgcgagttc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc 3960

3901 agtctccacc gctttgggct gtcctgatat ttctatggtc cgcaaagggg gaccttcgag 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc 4020

3961 ggagcacgcg agaggacaag gctgggacgg cgaatggcct atggacaggc ggaaagaggg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt 4080

4021 aagcccttcg caccgcgaaa gagtatcgag tgcgacatcc atagagtcaa gccacatcca 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt 4140

4081 gcaagcgagg ttcgacccga cacacgtgct tggggggcaa gtcgggctgg cgacgcggaa 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc 4200

4141 taggccattg atagcagaac tcaggttggg ccattctgtg ctgaatagcg gtgaccgtcg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa 4260

4201 tcggtgacca ttgtcctaat cgtctcgctc catacatccg ccacgatgtc tcaagaactt 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg ctctgctgaa 4320

4261 caccaccgga ttgatgccga tgtgatcttc ctgtcataaa ccatagacgc gagacgactt 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg 4380

4321 cggtcaatgg aagccttttt ctcaaccatc gagaactagg ccgtttgttt ggtggcgacc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga 4440

4381 atcgccacca aaaaaacaaa cgttcgtcgt ctaatgcgcg tctttttttc ctagagttct 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg 4500

4441 tctaggaaac tagaaaagat gccccagact gcgagtcacc ttgcttttga gtgcaattcc 4500

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg 4560

4501 ctaaaaccag tactctaata gtttttccta gaagtggatc taggaaaatt taatttttac 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt 4620

4561 ttcaaaattt agttagattt catatatact catttgaacc agactgtcaa tggttacgaa 4620

.......... .......... .......... .......... ....... ORF 1 Ampicillin

.......... .......... .......... .......... .......... ..........

4621 aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact 4680

4621 ttagtcactc cgtggataga gtcgctagac agataaagca agtaggtatc aacggactga 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat 4740

4681 ggggcagcac atctattgat gctatgccct cccgaatggt agaccggggt cacgacgtta 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg 4800

4741 ctatggcgct ctgggtgcga gtggccgagg tctaaatagt cgttatttgg tcggtcggcc 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg 4860

4801 ttcccggctc gcgtcttcac caggacgttg aaataggcgg aggtaggtca gataattaac 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat 4920

4861 aacggccctt cgatctcatt catcaagcgg tcaattatca aacgcgttgc aacaacggta 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

PstI(4927) .......... .......... .......... .......... ..........

4921 tgctgcaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc 4980

4921 acgacgtccg tagcaccaca gtgcgagcag caaaccatac cgaagtaagt cgaggccaag 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt 5040

4981 ggttgctagt tccgctcaat gtactagggg gtacaacacg ttttttcgcc aatcgaggaa 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc 5100

5041 gccaggaggc tagcaacagt cttcattcaa ccggcgtcac aatagtgagt accaataccg 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga 5160

5101 tcgtgacgta ttaagagaat gacagtacgg taggcattct acgaaaagac actgaccact 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gtactcaacc aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc 5220

5161 catgagttgg ttcagtaaga ctcttatcac atacgccgct ggctcaacga gaacgggccg 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gtcaacacgg gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa 5280

5221 cagttgtgcc ctattatggc gcggtgtatc gtcttgaaat tttcacgagt agtaaccttt 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta 5340

5281 tgcaagaagc cccgcttttg agagttccta gaatggcgac aactctaggt caagctacat 5340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg 5400

5341 tgggtgagca cgtgggttga ctagaagtcg tagaaaatga aagtggtcgc aaagacccac 5400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg 5460

5401 tcgtttttgt ccttccgttt tacggcgttt tttcccttat tcccgctgtg cctttacaac 5460

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat 5520

5461 ttatgagtat gagaaggaaa aagttataat aacttcgtaa atagtcccaa taacagagta 5520

ORF 1 Ampicillin .... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

5521 gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt 5580

5521 ctcgcctatg tataaactta cataaatctt tttatttgtt tatccccaag gcgcgtgtaa 5580

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat taacctataa 5640

5581 aggggctttt cacggtggac tgcagattct ttggtaataa tagtactgta attggatatt 5640

.......... .......... .......... .......... .......... ..........

.......... .......... ......... EcoRI(5675) ......... ..........

5641 aaataggcgt atcacgaggc cctttcgtct tcaagaa... .......... .......... 5677

5641 tttatccgca tagtgctccg ggaaagcaga agttctt... .......... .......... 5677

.......... .......... .......... .......... .......... ..........