Sequence of pET11d

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Sequence of pET11d

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Sequence of pET11d : 5674 BP; 1298 A; 1549 C; 1496 G; 1331 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 240

181 cgccctatag gcctatatca aggaggaaag tcgttttttg gggagttctg ggcaaatctc 240

.......... .......... .......... T7_term .......... ..........

.......... .......... .......... .......... .......... ..........

241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 300

241 cggggttccc caatacgatc aataacgagt cgccaccgtc gtcggttgag tcgaaggaaa 300

.......... ... T7_term T7_Terminal_pri ... .......... ..........

.......... BamHI(319) .......... .......... .......... ..........

301 cgggctttgt tagcagccgg atccgaccca tttgctgtcc accagtcatg ctagccatgg 360

301 gcccgaaaca atcgtcggcc taggctgggt aaacgacagg tggtcagtac gatcggtacc 360

T7_term .......... .......... .. T7_gene10_leader T7_leader ..

.......... .......... .......... .......... .......... ..........

361 tatatctcct tcttaaagtt aaacaaaatt atttctagag gggaattgtt atccgctcac 420

361 atatagagga agaatttcaa tttgttttaa taaagatctc cccttaacaa taggcgagtg 420

....... T7_transl_en_RBS ....... .......... lacO ..........

.......... .......... .......... BglII(460) .......... ..........

421 aattccccta tagtgagtcg tattaatttc gcgggatcga gatctcgatc ctctacgccg 480

421 ttaaggggat atcactcagc ataattaaag cgccctagct ctagagctag gagatgcggc 480

.......... T7_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gacgcatcgt ggccggcatc accggcgcca caggtgcggt tgctggcgcc tatatcgccg 540

481 ctgcgtagca ccggccgtag tggccgcggt gtccacgcca acgaccgcgg atatagcggc 540

.......... .......... .......... .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

541 acatcaccga tggggaagat cgggctcgcc acttcgggct catgagcgct tgtttcggcg 600

541 tgtagtggct accccttcta gcccgagcgg tgaagcccga gtactcgcga acaaagccgc 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tgggtatggt ggcaggcccc gtggccgggg gactgttggg cgccatctcc ttgcatgcac 660

601 acccatacca ccgtccgggg caccggcccc ctgacaaccc gcggtagagg aacgtacgtg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cattccttgc ggcggcggtg ctcaacggcc tcaacctact actgggctgc ttcctaatgc 720

661 gtaaggaacg ccgccgccac gagttgccgg agttggatga tgacccgacg aaggattacg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 aggagtcgca taagggagag cgtcgagatc ccggacacca tcgaatggcg caaaaccttt 780

721 tcctcagcgt attccctctc gcagctctag ggcctgtggt agcttaccgc gttttggaaa 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cgcggtatgg catgatagcg cccggaagag agtcaattca gggtggtgaa tgtgaaacca 840

781 gcgccatacc gtactatcgc gggccttctc tcagttaagt cccaccactt acactttggt 840

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

841 gtaacgttat acgatgtcgc agagtatgcc ggtgtctctt atcagaccgt ttcccgcgtg 900

841 cattgcaata tgctacagcg tctcatacgg ccacagagaa tagtctggca aagggcgcac 900

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gtgaaccagg ccagccacgt ttctgcgaaa acgcgggaaa aagtggaagc ggcgatggcg 960

901 cacttggtcc ggtcggtgca aagacgcttt tgcgcccttt ttcaccttcg ccgctaccgc 960

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gagctgaatt acattcccaa ccgcgtggca caacaactgg cgggcaaaca gtcgttgctg 1020

961 ctcgacttaa tgtaagggtt ggcgcaccgt gttgttgacc gcccgtttgt cagcaacgac 1020

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 attggcgttg ccacctccag tctggccctg cacgcgccgt cgcaaattgt cgcggcgatt 1080

1021 taaccgcaac ggtggaggtc agaccgggac gtgcgcggca gcgtttaaca gcgccgctaa 1080

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aaatctcgcg ccgatcaact gggtgccagc gtggtggtgt cgatggtaga acgaagcggc 1140

1081 tttagagcgc ggctagttga cccacggtcg caccaccaca gctaccatct tgcttcgccg 1140

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1196)

1141 gtcgaagcct gtaaagcggc ggtgcacaat cttctcgcgc aacgcgtcag tgggctgatc 1200

1141 cagcttcgga catttcgccg ccacgtgtta gaagagcgcg ttgcgcagtc acccgactag 1200

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 attaactatc cgctggatga ccaggatgcc attgctgtgg aagctgcctg cactaatgtt 1260

1201 taattgatag gcgacctact ggtcctacgg taacgacacc ttcgacggac gtgattacaa 1260

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ccggcgttat ttcttgatgt ctctgaccag acacccatca acagtattat tttctcccat 1320

1261 ggccgcaata aagaactaca gagactggtc tgtgggtagt tgtcataata aaagagggta 1320

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gaagacggta cgcgactggg cgtggagcat ctggtcgcat tgggtcacca gcaaatcgcg 1380

1321 cttctgccat gcgctgaccc gcacctcgta gaccagcgta acccagtggt cgtttagcgc 1380

.......... .......... lacI ORF 1 .......... .......... ..........

.......... ApaI(1393) .......... .......... .......... ..........

1381 ctgttagcgg gcccattaag ttctgtctcg gcgcgtctgc gtctggctgg ctggcataaa 1440

1381 gacaatcgcc cgggtaattc aagacagagc cgcgcagacg cagaccgacc gaccgtattt 1440

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tatctcactc gcaatcaaat tcagccgata gcggaacggg aaggcgactg gagtgccatg 1500

1441 atagagtgag cgttagttta agtcggctat cgccttgccc ttccgctgac ctcacggtac 1500

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tccggttttc aacaaaccat gcaaatgctg aatgagggca tcgttcccac tgcgatgctg 1560

1501 aggccaaaag ttgtttggta cgtttacgac ttactcccgt agcaagggtg acgctacgac 1560

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gttgccaacg atcagatggc gctgggcgca atgcgcgcca ttaccgagtc cgggctgcgc 1620

1561 caacggttgc tagtctaccg cgacccgcgt tacgcgcggt aatggctcag gcccgacgcg 1620

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gttggtgcgg atatctcggt agtgggatac gacgataccg aagacagctc atgttatatc 1680

1621 caaccacgcc tatagagcca tcaccctatg ctgctatggc ttctgtcgag tacaatatag 1680

.......... .......... lacI ORF 1 .......... .......... ..........

HpaI(1688) .......... .......... .......... .......... ..........

1681 ccgccgttaa ccaccatcaa acaggatttt cgcctgctgg ggcaaaccag cgtggaccgc 1740

1681 ggcggcaatt ggtggtagtt tgtcctaaaa gcggacgacc ccgtttggtc gcacctggcg 1740

.......... .......... lacI ORF 1 ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttgctgcaac tctctcaggg ccaggcggtg aagggcaatc agctgttgcc cgtctcactg 1800

1741 aacgacgttg agagagtccc ggtccgccac ttcccgttag tcgacaacgg gcagagtgac 1800

.......... ....... lacI ORF 1 ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gtgaaaagaa aaaccaccct ggcgcccaat acgcaaaccg cctctccccg cgcgttggcc 1860

1801 cacttttctt tttggtggga ccgcgggtta tgcgtttggc ggagaggggc gcgcaaccgg 1860

.......... ....... lacI ORF 1 ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa 1920

1861 ctaagtaatt acgtcgaccg tgctgtccaa agggctgacc tttcgcccgt cactcgcgtt 1920

.......... ....... lacI ORF 1 ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 cgcaattaat gtaagttagc tcactcatta ggcaccggga tctcgaccga tgcccttgag 1980

1921 gcgttaatta cattcaatcg agtgagtaat ccgtggccct agagctggct acgggaactc 1980

.......... .......... ORF 2 .......... .......... ORF 2

.......... .......... .......... .......... .......... ..........

1981 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact 2040

1981 tcggaagttg ggtcagtcga ggaaggccac ccgcgccccg tactgatagc agcggcgtga 2040

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat 2100

2041 atactgacag aagaaatagt acgttgagca tcctgtccac ggccgtcgcg agacccagta 2100

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt 2160

2101 aaagccgctc ctggcgaaag cgacctcgcg ctgctactag ccggacagcg aacgccataa 2160

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg 2220

2161 gccttagaac gtgcgggagc gagttcggaa gcagtgacca gggcggtggt ttgcaaagcc 2220

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc 2280

2221 gctcttcgtc cggtaatagc ggccgtaccg ccggctgcgc gacccgatgc agaacgaccg 2280

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

NruI(2285) .......... .......... .......... .......... ..........

2281 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat 2340

2281 caagcgctgc gctccgacct accggaaggg gtaatactaa gaagagcgaa ggccgccgta 2340

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca 2400

2341 gccctacggg cgcaacgtcc ggtacgacag gtccgtccat ctactgctgg tagtccctgt 2400

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac cgctgatcgt 2460

2401 cgaagttcct agcgagcgcc gagaatggtc ggattgaagc tagtgacctg gcgactagca 2460

.......... ..... ORF 2 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc 2520

2461 gtgccgctaa atacggcgga gccgctcgtg taccttgccc aaccgtacct aacatccgcg 2520

.......... ..... ORF 2 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc 2580

2521 gcgggatatg gaacagacgg aggggcgcaa cgcagcgcca cgtacctcgg cccggtggag 2580

.......... ..... ORF 2 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc 2640

2581 ctggacttac cttcggccgc cgtggagcga ttgcctaagt ggtgaggttc ttaacctcgg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc 2700

2641 ttagttaaga acgcctcttg acacttacgc gtttggttgg gaaccgtctt gtataggtag 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... MscI(2757)

2701 gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca 2760

2701 cgcaggcggt agaggtcgtc ggcgtgcgcc gcgtagagcc cgtcgcaacc caggaccggt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt 2820

2761 gcccacgcgt actagcacga ggacagcaac tcctgggccg atccgaccgc cccaacggaa 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa 2880

2821 tgaccaatcg tcttacttag tggctatgcg ctcgcttgca cttcgctgac gacgacgttt 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg 2940

2881 tgcagacgct ggactcgttg ttgtacttac cagaagccaa aggcacaaag catttcagac 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc aggatgctgc 3000

2941 ctttgcgcct tcagtcgcgg gacgtggtaa tacaaggcct agacgtagcg tcctacgacg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg accctgagtg 3060

3001 accgatggga caccttgtgg atgtagacat aattgcttcg cgaccgtaac tgggactcac 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca acgttccagt 3120

3061 taaaaagaga ccagggcggc gtaggtatgg cggtcaacaa atgggagtgt tgcaaggtca 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg tttcatcggt 3180

3121 ttggcccgta caagtagtag tcattgggca tagcactcgt aggagagagc aaagtagcca 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac caaacaggaa 3240

3181 tagtaatggg ggtacttgtc tttaggggga atgtgcctcc gtagtcactg gtttgtcctt 3240

.......... .......... .......... .......... .......... ROP

.......... .......... .......... .......... .......... ..........

3241 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa 3300

3241 ttttggcggg aattgtaccg ggcgaaatag tcttcggtct gtaattgcga agacctcttt 3300

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct 3360

3301 gagttgctcg acctgcgcct acttgtccgt ctgtagacac ttagcgaagt gctggtgcga 3360

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 3420

3361 ctactcgaaa tggcgtcgac ggagcgcgca aagccactac tgccactttt ggagactgtg 3420

.......... .......... ROP .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

3421 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 3480

3421 tacgtcgagg gcctctgcca gtgtcgaaca gacattcgcc tacggccctc gtctgttcgg 3480

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt 3540

3481 gcagtcccgc gcagtcgccc acaaccgccc acagccccgc gtcggtactg ggtcagtgca 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag 3600

3541 tcgctatcgc ctcacatatg accgaattga tacgccgtag tctcgtctaa catgactctc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat accgcatcag 3660

3601 acgtggtata tacgccacac tttatggcgt gtctacgcat tcctctttta tggcgtagtc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc 3720

3661 cgcgagaagg cgaaggagcg agtgactgag cgacgcgagc cagcaagccg acgccgctcg 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg 3780

3721 ccatagtcga gtgagtttcc gccattatgc caataggtgt cttagtcccc tattgcgtcc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct 3840

3781 tttcttgtac actcgttttc cggtcgtttt ccggtccttg gcatttttcc ggcgcaacga 3840

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

3841 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca 3900

3841 ccgcaaaaag gtatccgagg cggggggact gctcgtagtg tttttagctg cgagttcagt 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct 3960

3901 ctccaccgct ttgggctgtc ctgatatttc tatggtccgc aaagggggac cttcgaggga 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc 4020

3961 gcacgcgaga ggacaaggct gggacggcga atggcctatg gacaggcgga aagagggaag 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt 4080

4021 cccttcgcac cgcgaaagag tatcgagtgc gacatccata gagtcaagcc acatccagca 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc 4140

4081 agcgaggttc gacccgacac acgtgcttgg ggggcaagtc gggctggcga cgcggaatag 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc 4200

4141 gccattgata gcagaactca ggttgggcca ttctgtgctg aatagcggtg accgtcgtcg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg 4260

4201 gtgaccattg tcctaatcgt ctcgctccat acatccgcca cgatgtctca agaacttcac 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc 4320

4261 caccggattg atgccgatgt gatcttcctg tcataaacca tagacgcgag acgacttcgg 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag 4380

4321 tcaatggaag cctttttctc aaccatcgag aactaggccg tttgtttggt ggcgaccatc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga 4440

4381 gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct ttttttccta gagttcttct 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat 4500

4441 aggaaactag aaaagatgcc ccagactgcg agtcaccttg cttttgagtg caattcccta 4500

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag 4560

4501 aaaccagtac tctaatagtt tttcctagaa gtggatctag gaaaatttaa tttttacttc 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat 4620

4561 aaaatttagt tagatttcat atatactcat ttgaaccaga ctgtcaatgg ttacgaatta 4620

.......... .......... .......... .......... ....... ORF 1 Ampicillin

.......... .......... .......... .......... .......... ..........

4621 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc 4680

4621 gtcactccgt ggatagagtc gctagacaga taaagcaagt aggtatcaac ggactgaggg 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat 4740

4681 gcagcacatc tattgatgct atgccctccc gaatggtaga ccggggtcac gacgttacta 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag 4800

4741 tggcgctctg ggtgcgagtg gccgaggtct aaatagtcgt tatttggtcg gtcggccttc 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg 4860

4801 ccggctcgcg tcttcaccag gacgttgaaa taggcggagg taggtcagat aattaacaac 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc 4920

4861 ggcccttcga tctcattcat caagcggtca attatcaaac gcgttgcaac aacggtaacg 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

PstI(4924) .......... .......... .......... .......... ..........

4921 tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca 4980

4921 acgtccgtag caccacagtg cgagcagcaa accataccga agtaagtcga ggccaagggt 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg 5040

4981 tgctagttcc gctcaatgta ctagggggta caacacgttt tttcgccaat cgaggaagcc 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc 5100

5041 aggaggctag caacagtctt cattcaaccg gcgtcacaat agtgagtacc aataccgtcg 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta 5160

5101 tgacgtatta agagaatgac agtacggtag gcattctacg aaaagacact gaccactcat 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc 5220

5161 gagttggttc agtaagactc ttatcacata cgccgctggc tcaacgagaa cgggccgcag 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg 5280

5221 ttgtgcccta ttatggcgcg gtgtatcgtc ttgaaatttt cacgagtagt aaccttttgc 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc 5340

5281 aagaagcccc gcttttgaga gttcctagaa tggcgacaac tctaggtcaa gctacattgg 5340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc 5400

5341 gtgagcacgt gggttgacta gaagtcgtag aaaatgaaag tggtcgcaaa gacccactcg 5400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat 5460

5401 tttttgtcct tccgttttac ggcgtttttt cccttattcc cgctgtgcct ttacaactta 5460

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag 5520

5461 tgagtatgag aaggaaaaag ttataataac ttcgtaaata gtcccaataa cagagtactc 5520

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

5521 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc 5580

5521 gcctatgtat aaacttacat aaatcttttt atttgtttat ccccaaggcg cgtgtaaagg 5580

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa 5640

5581 ggcttttcac ggtggactgc agattctttg gtaataatag tactgtaatt ggatattttt 5640

.......... .......... .......... .......... .......... ..........

.......... .......... ......... EcoRI(5672) ......... ..........

5641 taggcgtatc acgaggccct ttcgtcttca agaa...... .......... .......... 5674

5641 atccgcatag tgctccggga aagcagaagt tctt...... .......... .......... 5674

.......... .......... .......... .......... .......... ..........