Sequence of pET12a

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pET12a

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pET12a : 4674 BP; 1059 A; 1285 C; 1208 G; 1122 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... ......... tet(w/ gaps) .........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... NheI(229) ..........

181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 240

181 cgccctatag caggtaaggc tgtcgtagcg gtcagtgata ccgcacgacg atcgcgatat 240

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 300

241 acgcaactac gttaaagata cgcgtgggca agagcctcgt gacaggctgg cgaaaccggc 300

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 360

301 ggcgggtcag gacgagcgaa gcgatgaacc tcggtgatag ctgatgcgct agtaccgctg 360

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 420

361 gtgtgggcag gacacctata ggcctatatc aaggaggaaa gtcgtttttt ggggagttct 420

.......... ......... tet(w/ gaps) T7_term ......... ..........

.......... .......... .......... .......... .......... ..........

421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 480

421 gggcaaatct ccggggttcc ccaatacgat caataacgag tcgccaccgt cgtcggttga 480

.......... .. T7_term tet(w/ gaps) T7_Terminal_pri .. ..........

.......... .......... BamHI(510) .......... .......... ..........

481 cagcttcctt tcgggctttg ttagcagccg gatcccgtcg acgcaaaaga gctgatcgcg 540

481 gtcgaaggaa agcccgaaac aatcgtcggc ctagggcagc tgcgttttct cgactagcgc 540

......... T7_term tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ataggggttg tcaggactat tcctaggagt ttcgcccgca tatgtatatc tccttcttaa 600

541 tatccccaac agtcctgata aggatcctca aagcgggcgt atacatatag aggaagaatt 600

.......... ......... tet(w/ gaps) ......... ....... T7_transl_en_RBS

.......... XbaI(618) .......... .......... .......... ..........

601 agttaaacaa aattatttct agagggaaac cgttgtggtc tccctatagt gagtcgtatt 660

601 tcaatttgtt ttaataaaga tctccctttg gcaacaccag agggatatca ct cagcataa 660

.......... ......... tet(w/ gaps) ......... .......... T7_pro

.......... BglII(676) .......... .......... .......... ..........

661 aatttcgcgg gatcgagatc tcgatcctct acgccggacg catcgtggcc ggcatcaccg 720

661 ttaaagcgcc ctagctctag agctaggaga tgcggcctgc gtagcaccgg ccgtagtggc 720

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcgccacagg tgcggttgct ggcgcctata tcgccgacat caccgatggg gaagatcggg 780

721 cgcggtgtcc acgccaacga ccgcggatat agcggctgta gtggctaccc cttctagccc 780

.......... ....... tet(w/ gaps) pBRrevBam_pri ....... ..........

.......... .......... .......... .......... .......... ..........

781 ctcgccactt cgggctcatg agcgcttgtt tcggcgtggg tatggtggca ggccccgtgg 840

781 gagcggtgaa gcccgagtac tcgcgaacaa agccgcaccc ataccaccgt ccggggcacc 840

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ccgggggact gttgggcgcc atctccttgc atgcaccatt ccttgcggcg gcggtgctca 900

841 ggccccctga caacccgcgg tagaggaacg tacgtggtaa ggaacgccgc cgccacgagt 900

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 acggcctcaa cctactactg ggctgcttcc taatgcagga gtcgcataag ggagagcgtc 960

901 tgccggagtt ggatgatgac ccgacgaagg attacgtcct cagcgtattc cctctcgcag 960

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gatcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 1020

961 ctagctggct acgggaactc tcggaagttg ggtcagtcga ggaaggccac ccgcgccccg 1020

.......... ......... tet(w/ gaps) ORF 1 ......... ..........

.......... .......... .......... .......... .......... ..........

1021 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg 1080

1021 tactgatagc agcggcgtga atactgacag aagaaatagt acgttgagca tcctgtccac 1080

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc 1140

1081 ggccgtcgcg agacccagta aaagccgctc ctggcgaaag cgacctcgcg ctgctactag 1140

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt 1200

1141 ccggacagcg aacgccataa gccttagaac gtgcgggagc gagttcggaa gcagtgacca 1200

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... EagI(1250) ..........

1201 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg 1260

1201 gggcggtggt ttgcaaagcc gctcttcgtc cggtaatagc ggccgtaccg ccggctgcgc 1260

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt 1320

1261 gacccgatgc agaacgaccg caagcgctgc gctccgacct accggaaggg gtaatactaa 1320

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta 1380

1321 gaagagcgaa ggccgccgta gccctacggg cgcaacgtcc ggtacgacag gtccgtccat 1380

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg 1440

1381 ctactgctgg tagtccctgt cgaagttcct agcgagcgcc gagaatggtc ggattgaagc 1440

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg 1500

1441 tagtgacctg gcgactagca gtgccgctaa atacggcgga gccgctcgtg taccttgccc 1500

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt 1560

1501 aaccgtacct aacatccgcg gcgggatatg gaacagacgg aggggcgcaa cgcagcgcca 1560

.......... ...... tet(w/ gaps) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca 1620

1561 cgtacctcgg cccggtggag ctggacttac cttcggccgc cgtggagcga ttgcctaagt 1620

...... tet(w/ gaps) ORF 1 ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc 1680

1621 ggtgaggttc ttaacctcgg ttagttaaga acgcctcttg acacttacgc gtttggttgg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg 1740

1681 gaaccgtctt gtataggtag cgcaggcggt agaggtcgtc ggcgtgcgcc gcgtagagcc 1740

.......... .......... .......... .......... .......... ..........

.......... MscI(1757) .......... .......... .......... ..........

1741 gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc 1800

1741 cgtcgcaacc caggaccggt gcccacgcgt actagcacga ggacagcaac tcctgggccg 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt 1860

1801 atccgaccgc cccaacggaa tgaccaatcg tcttacttag tggctatgcg ctcgcttgca 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt 1920

1861 cttcgctgac gacgacgttt tgcagacgct ggactcgttg ttgtacttac cagaagccaa 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga 1980

1921 aggcacaaag catttcagac ctttgcgcct tcagtcgcgg gacgtggtaa tacaaggcct 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc 2040

1981 agacgtagcg tcctacgacg accgatggga caccttgtgg atgtagacat aattgcttcg 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt 2100

2041 cgaccgtaac tgggactcac taaaaagaga ccagggcggc gtaggtatgg cggtcaacaa 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca 2160

2101 atgggagtgt tgcaaggtca ttggcccgta caagtagtag tcattgggca tagcactcgt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg 2220

2161 aggagagagc aaagtagcca tagtaatggg ggtacttgtc tttaggggga atgtgcctcc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga 2280

2221 gtagtcactg gtttgtcctt ttttggcggg aattgtaccg ggcgaaatag tcttcggtct 2280

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg 2340

2281 gtaattgcga agacctcttt gagttgctcg acctgcgcct acttgtccgt ctgtagacac 2340

.......... .......... ROP .......... .......... ..........

.......... .......... ......... PvuII(2377) ......... ..........

2341 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg 2400

2341 ttagcgaagt gctggtgcga ctactcgaaa tggcgtcgac ggagcgcgca aagccactac 2400

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 2460

2401 tgccactttt ggagactgtg tacgtcgagg gcctctgcca gtgtcgaaca gacattcgcc 2460

ROP .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg 2520

2461 tacggccctc gtctgttcgg gcagtcccgc gcagtcgccc acaaccgccc acagccccgc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc 2580

2521 gtcggtactg ggtcagtgca tcgctatcgc ctcacatatg accgaattga tacgccgtag 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta 2640

2581 tctcgtctaa catgactctc acgtggtata tacgccacac tttatggcgt gtctacgcat 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 2700

2641 tcctctttta tggcgtagtc cgcgagaagg cgaaggagcg agtgactgag cgacgcgagc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 2760

2701 cagcaagccg acgccgctcg ccatagtcga gtgagtttcc gccattatgc caataggtgt 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 2820

2761 cttagtcccc tattgcgtcc tttcttgtac actcgttttc cggtcgtttt ccggtccttg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 2880

2821 gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg cggggggact gctcgtagtg 2880

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2881 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 2940

2881 tttttagctg cgagttcagt ctccaccgct ttgggctgtc ctgatatttc tatggtccgc 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 3000

2941 aaagggggac cttcgaggga gcacgcgaga ggacaaggct gggacggcga atggcctatg 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 3060

3001 gacaggcgga aagagggaag cccttcgcac cgcgaaagag tatcgagtgc gacatccata 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 3120

3061 gagtcaagcc acatccagca agcgaggttc gacccgacac acgtgcttgg ggggcaagtc 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 3180

3121 gggctggcga cgcggaatag gccattgata gcagaactca ggttgggcca ttctgtgctg 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 3240

3181 aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat acatccgcca 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 3300

3241 cgatgtctca agaacttcac caccggattg atgccgatgt gatcttcctg tcataaacca 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 3360

3301 tagacgcgag acgacttcgg tcaatggaag cctttttctc aaccatcgag aactaggccg 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 3420

3361 tttgtttggt ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 3480

3421 ttttttccta gagttcttct aggaaactag aaaagatgcc ccagactgcg agtcaccttg 3480

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 3540

3481 cttttgagtg caattcccta aaaccagtac tctaatagtt tttcctagaa gtggatctag 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 3600

3541 gaaaatttaa tttttacttc aaaatttagt tagatttcat atatactcat ttgaaccaga 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 3660

3601 ctgtcaatgg ttacgaatta gtcactccgt ggatagagtc gctagacaga taaagcaagt 3660

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3661 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 3720

3661 aggtatcaac ggactgaggg gcagcacatc tattgatgct atgccctccc gaatggtaga 3720

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 3780

3721 ccggggtcac gacgttacta tggcgctctg ggtgcgagtg gccgaggtct aaatagtcgt 3780

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 3840

3781 tatttggtcg gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa taggcggagg 3840

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 3900

3841 taggtcagat aattaacaac ggcccttcga tctcattcat caagcggtca attatcaaac 3900

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... PstI(3924) .......... .......... ..........

3901 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 3960

3901 gcgttgcaac aacggtaacg acgtccgtag caccacagtg cgagcagcaa accataccga 3960

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 4020

3961 agtaagtcga ggccaagggt tgctagttcc gctcaatgta ctagggggta caacacgttt 4020

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 4080

4021 tttcgccaat cgaggaagcc aggaggctag caacagtctt cattcaaccg gcgtcacaat 4080

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 4140

4081 agtgagtacc aataccgtcg tgacgtatta agagaatgac agtacggtag gcattctacg 4140

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 4200

4141 aaaagacact gaccactcat gagttggttc agtaagactc ttatcacata cgccgctggc 4200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 4260

4201 tcaacgagaa cgggccgcag ttgtgcccta ttatggcgcg gtgtatcgtc ttgaaatttt 4260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 4320

4261 cacgagtagt aaccttttgc aagaagcccc gcttttgaga gttcctagaa tggcgacaac 4320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 4380

4321 tctaggtcaa gctacattgg gtgagcacgt gggttgacta gaagtcgtag aaaatgaaag 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 4440

4381 tggtcgcaaa gacccactcg tttttgtcct tccgttttac ggcgtttttt cccttattcc 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 4500

4441 cgctgtgcct ttacaactta tgagtatgag aaggaaaaag ttataataac ttcgtaaata 4500

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 4560

4501 gtcccaataa cagagtactc gcctatgtat aaacttacat aaatcttttt atttgtttat 4560

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 4620

4561 ccccaaggcg cgtgtaaagg ggcttttcac ggtggactgc agattctttg gtaataatag 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... EcoRI(4672)

4621 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa...... 4674

4621 tactgtaatt ggatattttt atccgcatag tgctccggga aagcagaagt tctt...... 4674

.......... .......... .......... .......... .......... ..........