Sequence of pET12b

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Sequence of pET12b

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Sequence of pET12b : 4673 BP; 1059 A; 1284 C; 1208 G; 1122 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... ......... tet(w/ gaps) .........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... NheI(229) ..........

181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 240

181 cgccctatag caggtaaggc tgtcgtagcg gtcagtgata ccgcacgacg atcgcgatat 240

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 300

241 acgcaactac gttaaagata cgcgtgggca agagcctcgt gacaggctgg cgaaaccggc 300

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 360

301 ggcgggtcag gacgagcgaa gcgatgaacc tcggtgatag ctgatgcgct agtaccgctg 360

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 420

361 gtgtgggcag gacacctata ggcctatatc aaggaggaaa gtcgtttttt ggggagttct 420

.......... ......... tet(w/ gaps) T7_term ......... ..........

.......... .......... .......... .......... .......... ..........

421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 480

421 gggcaaatct ccggggttcc ccaatacgat caataacgag tcgccaccgt cgtcggttga 480

.......... .. T7_term tet(w/ gaps) T7_Terminal_pri .. ..........

.......... .......... BamHI(510) .......... .......... ..........

481 cagcttcctt tcgggctttg ttagcagccg gatccgtcga cgcaaaagag ctgatcgcga 540

481 gtcgaaggaa agcccgaaac aatcgtcggc ctaggcagct gcgttttctc gactagcgct 540

......... T7_term tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 taggggttgt caggactatt cctaggagtt tcgcccgcat atgtatatct ccttcttaaa 600

541 atccccaaca gtcctgataa ggatcctcaa agcgggcgta tacatataga ggaagaattt 600

.......... ......... tet(w/ gaps) ......... ....... T7_transl_en_RBS

.......... XbaI(617) .......... .......... .......... ..........

601 gttaaacaaa attatttcta gagggaaacc gttgtggtct ccctatagtg agtcgtatta 660

601 caatttgttt taataaagat ctccctttgg caacaccaga gggatatcac tc agcataat 660

.......... ......... tet(w/ gaps) ......... .......... T7_pro

.......... BglII(675) .......... .......... .......... ..........

661 atttcgcggg atcgagatct cgatcctcta cgccggacgc atcgtggccg gcatcaccgg 720

661 taaagcgccc tagctctaga gctaggagat gcggcctgcg tagcaccggc cgtagtggcc 720

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgccacaggt gcggttgctg gcgcctatat cgccgacatc accgatgggg aagatcgggc 780

721 gcggtgtcca cgccaacgac cgcggatata gcggctgtag tggctacccc ttctagcccg 780

.......... ....... tet(w/ gaps) pBRrevBam_pri ....... ..........

.......... .......... .......... .......... .......... ..........

781 tcgccacttc gggctcatga gcgcttgttt cggcgtgggt atggtggcag gccccgtggc 840

781 agcggtgaag cccgagtact cgcgaacaaa gccgcaccca taccaccgtc cggggcaccg 840

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cgggggactg ttgggcgcca tctccttgca tgcaccattc cttgcggcgg cggtgctcaa 900

841 gccccctgac aacccgcggt agaggaacgt acgtggtaag gaacgccgcc gccacgagtt 900

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cggcctcaac ctactactgg gctgcttcct aatgcaggag tcgcataagg gagagcgtcg 960

901 gccggagttg gatgatgacc cgacgaagga ttacgtcctc agcgtattcc ctctcgcagc 960

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 atcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca 1020

961 tagctggcta cgggaactct cggaagttgg gtcagtcgag gaaggccacc cgcgccccgt 1020

.......... ......... tet(w/ gaps) ORF 3 ......... ..........

.......... .......... .......... .......... .......... ..........

1021 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc 1080

1021 actgatagca gcggcgtgaa tactgacaga agaaatagta cgttgagcat cctgtccacg 1080

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg 1140

1081 gccgtcgcga gacccagtaa aagccgctcc tggcgaaagc gacctcgcgc tgctactagc 1140

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc 1200

1141 cggacagcga acgccataag ccttagaacg tgcgggagcg agttcggaag cagtgaccag 1200

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... EagI(1249) ..........

1201 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc 1260

1201 ggcggtggtt tgcaaagccg ctcttcgtcc ggtaatagcg gccgtaccgc cggctgcgcg 1260

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc 1320

1261 acccgatgca gaacgaccgc aagcgctgcg ctccgaccta ccggaagggg taatactaag 1320

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag 1380

1321 aagagcgaag gccgccgtag ccctacgggc gcaacgtccg gtacgacagg tccgtccatc 1380

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga 1440

1381 tactgctggt agtccctgtc gaagttccta gcgagcgccg agaatggtcg gattgaagct 1440

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt 1500

1441 agtgacctgg cgactagcag tgccgctaaa tacggcggag ccgctcgtgt accttgccca 1500

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg 1560

1501 accgtaccta acatccgcgg cgggatatgg aacagacgga ggggcgcaac gcagcgccac 1560

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac 1620

1561 gtacctcggc ccggtggagc tggacttacc ttcggccgcc gtggagcgat tgcctaagtg 1620

...... tet(w/ gaps) ORF 3 ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc 1680

1621 gtgaggttct taacctcggt tagttaagaa cgcctcttga cacttacgcg tttggttggg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg 1740

1681 aaccgtcttg tataggtagc gcaggcggta gaggtcgtcg gcgtgcgccg cgtagagccc 1740

.......... .......... .......... .......... .......... ..........

.......... MscI(1756) .......... .......... .......... ..........

1741 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct 1800

1741 gtcgcaaccc aggaccggtg cccacgcgta ctagcacgag gacagcaact cctgggccga 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg 1860

1801 tccgaccgcc ccaacggaat gaccaatcgt cttacttagt ggctatgcgc tcgcttgcac 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt 1920

1861 ttcgctgacg acgacgtttt gcagacgctg gactcgttgt tgtacttacc agaagccaaa 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat 1980

1921 ggcacaaagc atttcagacc tttgcgcctt cagtcgcggg acgtggtaat acaaggccta 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg 2040

1981 gacgtagcgt cctacgacga ccgatgggac accttgtgga tgtagacata attgcttcgc 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt 2100

2041 gaccgtaact gggactcact aaaaagagac cagggcggcg taggtatggc ggtcaacaaa 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat 2160

2101 tgggagtgtt gcaaggtcat tggcccgtac aagtagtagt cattgggcat agcactcgta 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc 2220

2161 ggagagagca aagtagccat agtaatgggg gtacttgtct ttagggggaa tgtgcctccg 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac 2280

2221 tagtcactgg tttgtccttt tttggcggga attgtaccgg gcgaaatagt cttcggtctg 2280

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2281 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga 2340

2281 taattgcgaa gacctctttg agttgctcga cctgcgccta cttgtccgtc tgtagacact 2340

.......... .......... ROP .......... .......... ..........

.......... .......... ......... PvuII(2376) ......... ..........

2341 atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga 2400

2341 tagcgaagtg ctggtgcgac tactcgaaat ggcgtcgacg gagcgcgcaa agccactact 2400

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 2460

2401 gccacttttg gagactgtgt acgtcgaggg cctctgccag tgtcgaacag acattcgcct 2460

ROP .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc 2520

2461 acggccctcg tctgttcggg cagtcccgcg cagtcgccca caaccgccca cagccccgcg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca 2580

2521 tcggtactgg gtcagtgcat cgctatcgcc tcacatatga ccgaattgat acgccgtagt 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac agatgcgtaa 2640

2581 ctcgtctaac atgactctca cgtggtatat acgccacact ttatggcgtg tctacgcatt 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 2700

2641 cctcttttat ggcgtagtcc gcgagaaggc gaaggagcga gtgactgagc gacgcgagcc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 2760

2701 agcaagccga cgccgctcgc catagtcgag tgagtttccg ccattatgcc aataggtgtc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 2820

2761 ttagtcccct attgcgtcct ttcttgtaca ctcgttttcc ggtcgttttc cggtccttgg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 2880

2821 catttttccg gcgcaacgac cgcaaaaagg tatccgaggc ggggggactg ctcgtagtgt 2880

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2881 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 2940

2881 ttttagctgc gagttcagtc tccaccgctt tgggctgtcc tgatatttct atggtccgca 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 3000

2941 aagggggacc ttcgagggag cacgcgagag gacaaggctg ggacggcgaa tggcctatgg 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 3060

3001 acaggcggaa agagggaagc ccttcgcacc gcgaaagagt atcgagtgcg acatccatag 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 3120

3061 agtcaagcca catccagcaa gcgaggttcg acccgacaca cgtgcttggg gggcaagtcg 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 3180

3121 ggctggcgac gcggaatagg ccattgatag cagaactcag gttgggccat tctgtgctga 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 3240

3181 atagcggtga ccgtcgtcgg tgaccattgt cctaatcgtc tcgctccata catccgccac 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 3300

3241 gatgtctcaa gaacttcacc accggattga tgccgatgtg atcttcctgt cataaaccat 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 3360

3301 agacgcgaga cgacttcggt caatggaagc ctttttctca accatcgaga actaggccgt 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 3420

3361 ttgtttggtg gcgaccatcg ccaccaaaaa aacaaacgtt cgtcgtctaa tgcgcgtctt 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 3480

3421 tttttcctag agttcttcta ggaaactaga aaagatgccc cagactgcga gtcaccttgc 3480

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 3540

3481 ttttgagtgc aattccctaa aaccagtact ctaatagttt ttcctagaag tggatctagg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 3600

3541 aaaatttaat ttttacttca aaatttagtt agatttcata tatactcatt tgaaccagac 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 3660

3601 tgtcaatggt tacgaattag tcactccgtg gatagagtcg ctagacagat aaagcaagta 3660

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3661 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 3720

3661 ggtatcaacg gactgagggg cagcacatct attgatgcta tgccctcccg aatggtagac 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 3780

3721 cggggtcacg acgttactat ggcgctctgg gtgcgagtgg ccgaggtcta aatagtcgtt 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 3840

3781 atttggtcgg tcggccttcc cggctcgcgt cttcaccagg acgttgaaat aggcggaggt 3840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 3900

3841 aggtcagata attaacaacg gcccttcgat ctcattcatc aagcggtcaa ttatcaaacg 3900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... PstI(3923) .......... .......... ..........

3901 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 3960

3901 cgttgcaaca acggtaacga cgtccgtagc accacagtgc gagcagcaaa ccataccgaa 3960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 4020

3961 gtaagtcgag gccaagggtt gctagttccg ctcaatgtac tagggggtac aacacgtttt 4020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 4080

4021 ttcgccaatc gaggaagcca ggaggctagc aacagtcttc attcaaccgg cgtcacaata 4080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 4140

4081 gtgagtacca ataccgtcgt gacgtattaa gagaatgaca gtacggtagg cattctacga 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 4200

4141 aaagacactg accactcatg agttggttca gtaagactct tatcacatac gccgctggct 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag 4260

4201 caacgagaac gggccgcagt tgtgccctat tatggcgcgg tgtatcgtct tgaaattttc 4260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 4320

4261 acgagtagta accttttgca agaagccccg cttttgagag ttcctagaat ggcgacaact 4320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 4380

4321 ctaggtcaag ctacattggg tgagcacgtg ggttgactag aagtcgtaga aaatgaaagt 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 4440

4381 ggtcgcaaag acccactcgt ttttgtcctt ccgttttacg gcgttttttc ccttattccc 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 4500

4441 gctgtgcctt tacaacttat gagtatgaga aggaaaaagt tataataact tcgtaaatag 4500

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 4560

4501 tcccaataac agagtactcg cctatgtata aacttacata aatcttttta tttgtttatc 4560

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 4620

4561 cccaaggcgc gtgtaaaggg gcttttcacg gtggactgca gattctttgg taataatagt 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... EcoRI(4671)

4621 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaa....... 4673

4621 actgtaattg gatattttta tccgcatagt gctccgggaa agcagaagtt ctt....... 4673

.......... .......... .......... .......... .......... ..........