Sequence of pET12c

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Sequence of pET12c

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Sequence of pET12c : 4675 BP; 1059 A; 1286 C; 1208 G; 1122 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... ......... tet(w/ gaps) .........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... NheI(229) ..........

181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 240

181 cgccctatag caggtaaggc tgtcgtagcg gtcagtgata ccgcacgacg atcgcgatat 240

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 300

241 acgcaactac gttaaagata cgcgtgggca agagcctcgt gacaggctgg cgaaaccggc 300

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 360

301 ggcgggtcag gacgagcgaa gcgatgaacc tcggtgatag ctgatgcgct agtaccgctg 360

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 420

361 gtgtgggcag gacacctata ggcctatatc aaggaggaaa gtcgtttttt ggggagttct 420

.......... ......... tet(w/ gaps) T7_term ......... ..........

.......... .......... .......... .......... .......... ..........

421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 480

421 gggcaaatct ccggggttcc ccaatacgat caataacgag tcgccaccgt cgtcggttga 480

.......... .. T7_term tet(w/ gaps) T7_Terminal_pri .. ..........

.......... .......... BamHI(510) .......... .......... ..........

481 cagcttcctt tcgggctttg ttagcagccg gatccccgtc gacgcaaaag agctgatcgc 540

481 gtcgaaggaa agcccgaaac aatcgtcggc ctaggggcag ctgcgttttc tcgactagcg 540

......... T7_term tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gataggggtt gtcaggacta ttcctaggag tttcgcccgc atatgtatat ctccttctta 600

541 ctatccccaa cagtcctgat aaggatcctc aaagcgggcg tatacatata gaggaagaat 600

.......... ......... tet(w/ gaps) ......... ....... T7_transl_en_RBS

.......... XbaI(619) .......... .......... .......... ..........

601 aagttaaaca aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat 660

601 ttcaatttgt tttaataaag atctcccttt ggcaacacca gagggatatc ac tcagcata 660

.......... ......... tet(w/ gaps) ......... .......... T7_pro

.......... BglII(677) .......... .......... .......... ..........

661 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc 720

661 attaaagcgc cctagctcta gagctaggag atgcggcctg cgtagcaccg gccgtagtgg 720

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg 780

721 ccgcggtgtc cacgccaacg accgcggata tagcggctgt agtggctacc ccttctagcc 780

.......... ....... tet(w/ gaps) pBRrevBam_pri ....... ..........

.......... .......... .......... .......... .......... ..........

781 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg 840

781 cgagcggtga agcccgagta ctcgcgaaca aagccgcacc cataccaccg tccggggcac 840

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc 900

841 cggccccctg acaacccgcg gtagaggaac gtacgtggta aggaacgccg ccgccacgag 900

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt 960

901 ttgccggagt tggatgatga cccgacgaag gattacgtcc tcagcgtatt ccctctcgca 960

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgatcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg 1020

961 gctagctggc tacgggaact ctcggaagtt gggtcagtcg aggaaggcca cccgcgcccc 1020

.......... ......... tet(w/ gaps) ORF 2 ......... ..........

.......... .......... .......... .......... .......... ..........

1021 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt 1080

1021 gtactgatag cagcggcgtg aatactgaca gaagaaatag tacgttgagc atcctgtcca 1080

.......... ...... tet(w/ gaps) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat 1140

1081 cggccgtcgc gagacccagt aaaagccgct cctggcgaaa gcgacctcgc gctgctacta 1140

.......... ...... tet(w/ gaps) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg 1200

1141 gccggacagc gaacgccata agccttagaa cgtgcgggag cgagttcgga agcagtgacc 1200

.......... ...... tet(w/ gaps) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... EagI(1251)

1201 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc 1260

1201 agggcggtgg tttgcaaagc cgctcttcgt ccggtaatag cggccgtacc gccggctgcg 1260

.......... ...... tet(w/ gaps) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat 1320

1261 cgacccgatg cagaacgacc gcaagcgctg cgctccgacc taccggaagg ggtaatacta 1320

.......... ...... tet(w/ gaps) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt 1380

1321 agaagagcga aggccgccgt agccctacgg gcgcaacgtc cggtacgaca ggtccgtcca 1380

.......... ...... tet(w/ gaps) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc 1440

1381 tctactgctg gtagtccctg tcgaagttcc tagcgagcgc cgagaatggt cggattgaag 1440

.......... ...... tet(w/ gaps) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gatcactgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg 1500

1441 ctagtgacct ggcgactagc agtgccgcta aatacggcgg agccgctcgt gtaccttgcc 1500

.......... ...... tet(w/ gaps) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg 1560

1501 caaccgtacc taacatccgc ggcgggatat ggaacagacg gaggggcgca acgcagcgcc 1560

.......... ...... tet(w/ gaps) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc 1620

1561 acgtacctcg gcccggtgga gctggactta ccttcggccg ccgtggagcg attgcctaag 1620

...... tet(w/ gaps) ORF 2 ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac 1680

1621 tggtgaggtt cttaacctcg gttagttaag aacgcctctt gacacttacg cgtttggttg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccttggcaga acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg 1740

1681 ggaaccgtct tgtataggta gcgcaggcgg tagaggtcgt cggcgtgcgc cgcgtagagc 1740

.......... .......... .......... .......... .......... ..........

.......... MscI(1758) .......... .......... .......... ..........

1741 ggcagcgttg ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg 1800

1741 ccgtcgcaac ccaggaccgg tgcccacgcg tactagcacg aggacagcaa ctcctgggcc 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctaggctggc ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg 1860

1801 gatccgaccg ccccaacgga atgaccaatc gtcttactta gtggctatgc gctcgcttgc 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tgaagcgact gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt 1920

1861 acttcgctga cgacgacgtt ttgcagacgc tggactcgtt gttgtactta ccagaagcca 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ttccgtgttt cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg 1980

1921 aaggcacaaa gcatttcaga cctttgcgcc ttcagtcgcg ggacgtggta atacaaggcc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 atctgcatcg caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag 2040

1981 tagacgtagc gtcctacgac gaccgatggg acaccttgtg gatgtagaca taattgcttc 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cgctggcatt gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt 2100

2041 gcgaccgtaa ctgggactca ctaaaaagag accagggcgg cgtaggtatg gcggtcaaca 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttaccctcac aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc 2160

2101 aatgggagtg ttgcaaggtc attggcccgt acaagtagta gtcattgggc atagcactcg 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 atcctctctc gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag 2220

2161 taggagagag caaagtagcc atagtaatgg gggtacttgt ctttaggggg aatgtgcctc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcatcagtga ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag 2280

2221 cgtagtcact ggtttgtcct tttttggcgg gaattgtacc gggcgaaata gtcttcggtc 2280

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acattaacgc ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt 2340

2281 tgtaattgcg aagacctctt tgagttgctc gacctgcgcc tacttgtccg tctgtagaca 2340

.......... .......... ROP .......... .......... ..........

.......... .......... ......... PvuII(2378) ......... ..........

2341 gaatcgcttc acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat 2400

2341 cttagcgaag tgctggtgcg actactcgaa atggcgtcga cggagcgcgc aaagccacta 2400

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg 2460

2401 ctgccacttt tggagactgt gtacgtcgag ggcctctgcc agtgtcgaac agacattcgc 2460

ROP .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc 2520

2461 ctacggccct cgtctgttcg ggcagtcccg cgcagtcgcc cacaaccgcc cacagccccg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gcagccatga cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat 2580

2521 cgtcggtact gggtcagtgc atcgctatcg cctcacatat gaccgaattg atacgccgta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cagagcagat tgtactgaga gtgcaccata tatgcggtgt gaaataccgc acagatgcgt 2640

2581 gtctcgtcta acatgactct cacgtggtat atacgccaca ctttatggcg tgtctacgca 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 aaggagaaaa taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc 2700

2641 ttcctctttt atggcgtagt ccgcgagaag gcgaaggagc gagtgactga gcgacgcgag 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac 2760

2701 ccagcaagcc gacgccgctc gccatagtcg agtgagtttc cgccattatg ccaataggtg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa 2820

2761 tcttagtccc ctattgcgtc ctttcttgta cactcgtttt ccggtcgttt tccggtcctt 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca 2880

2821 ggcatttttc cggcgcaacg accgcaaaaa ggtatccgag gcggggggac tgctcgtagt 2880

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2881 caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc 2940

2881 gtttttagct gcgagttcag tctccaccgc tttgggctgt cctgatattt ctatggtccg 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata 3000

2941 caaaggggga ccttcgaggg agcacgcgag aggacaaggc tgggacggcg aatggcctat 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta 3060

3001 ggacaggcgg aaagagggaa gcccttcgca ccgcgaaaga gtatcgagtg cgacatccat 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca 3120

3061 agagtcaagc cacatccagc aagcgaggtt cgacccgaca cacgtgcttg gggggcaagt 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga 3180

3121 cgggctggcg acgcggaata ggccattgat agcagaactc aggttgggcc attctgtgct 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg 3240

3181 gaatagcggt gaccgtcgtc ggtgaccatt gtcctaatcg tctcgctcca tacatccgcc 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg 3300

3241 acgatgtctc aagaacttca ccaccggatt gatgccgatg tgatcttcct gtcataaacc 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg 3360

3301 atagacgcga gacgacttcg gtcaatggaa gcctttttct caaccatcga gaactaggcc 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag 3420

3361 gtttgtttgg tggcgaccat cgccaccaaa aaaacaaacg ttcgtcgtct aatgcgcgtc 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa 3480

3421 tttttttcct agagttcttc taggaaacta gaaaagatgc cccagactgc gagtcacctt 3480

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat 3540

3481 gcttttgagt gcaattccct aaaaccagta ctctaatagt ttttcctaga agtggatcta 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc 3600

3541 ggaaaattta atttttactt caaaatttag ttagatttca tatatactca tttgaaccag 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc 3660

3601 actgtcaatg gttacgaatt agtcactccg tggatagagt cgctagacag ataaagcaag 3660

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3661 atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc 3720

3661 taggtatcaa cggactgagg ggcagcacat ctattgatgc tatgccctcc cgaatggtag 3720

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc 3780

3721 accggggtca cgacgttact atggcgctct gggtgcgagt ggccgaggtc taaatagtcg 3780

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc 3840

3781 ttatttggtc ggtcggcctt cccggctcgc gtcttcacca ggacgttgaa ataggcggag 3840

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt 3900

3841 gtaggtcaga taattaacaa cggcccttcg atctcattca tcaagcggtc aattatcaaa 3900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... PstI(3925) .......... .......... ..........

3901 gcgcaacgtt gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc 3960

3901 cgcgttgcaa caacggtaac gacgtccgta gcaccacagt gcgagcagca aaccataccg 3960

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa 4020

3961 aagtaagtcg aggccaaggg ttgctagttc cgctcaatgt actagggggt acaacacgtt 4020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt 4080

4021 ttttcgccaa tcgaggaagc caggaggcta gcaacagtct tcattcaacc ggcgtcacaa 4080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg 4140

4081 tagtgagtac caataccgtc gtgacgtatt aagagaatga cagtacggta ggcattctac 4140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc 4200

4141 gaaaagacac tgaccactca tgagttggtt cagtaagact cttatcacat acgccgctgg 4200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gagttgctct tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa 4260

4201 ctcaacgaga acgggccgca gttgtgccct attatggcgc ggtgtatcgt cttgaaattt 4260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt 4320

4261 tcacgagtag taaccttttg caagaagccc cgcttttgag agttcctaga atggcgacaa 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt 4380

4321 ctctaggtca agctacattg ggtgagcacg tgggttgact agaagtcgta gaaaatgaaa 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag 4440

4381 gtggtcgcaa agacccactc gtttttgtcc ttccgtttta cggcgttttt tcccttattc 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta 4500

4441 ccgctgtgcc tttacaactt atgagtatga gaaggaaaaa gttataataa cttcgtaaat 4500

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat 4560

4501 agtcccaata acagagtact cgcctatgta taaacttaca taaatctttt tatttgttta 4560

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat 4620

4561 tccccaaggc gcgtgtaaag gggcttttca cggtggactg cagattcttt ggtaataata 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... EcoRI(4673)

4621 catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaa..... 4675

4621 gtactgtaat tggatatttt tatccgcata gtgctccggg aaagcagaag ttctt..... 4675

.......... .......... .......... .......... .......... ..........