Sequence of pET151_Directional

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Sequence of pET151_Directional_TOPO

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Sequence of pET151 Directional TOPO : 5760 BP; 1354 A; 1526 C; 1556 G; 1324 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa 60

1 gttcctctac cgcgggttgt cagggggccg gtgccccgga cggtggtatg ggtgcggctt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat 120

61 tgttcgcgag tactcgggct tcaccgctcg ggctagaagg ggtagccact acagccgcta 120

.......... .......... .......... .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta 180

121 tatccgcggt cgttggcgtg gacaccgcgg ccactacggc cggtgctacg caggccgcat 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg 240

181 ctcctagctc tagagctagg gcgctttaat tatgctgagt gatatcccct taacactcgc 240

.......... .......... .......... T7_pro .......... lacO

.......... XbaI(255) .......... .......... .......... ..........

241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat atacatatgc 300

241 ctattgttaa ggggagatct ttattaaaac aaattgaaat tcttcctcta tatgtatacg 300

lacO .......... ....... T7_transl_en_RBS ....... ..........

.......... NcoI(314) .......... .......... .......... ..........

301 atcatcacca tcaccatggt aagcctatcc ctaaccctct cctcggtctc gattctacgg 360

301 tagtagtggt agtggtacca ttcggatagg gattgggaga ggagccagag ctaagatgcc 360

6xHis .......... .......... V5 .......... ..........

.......... .......... .......... .......... SacI(406) ..........

361 aaaacctgta ttttcaggga attgatccct tcaccaaggg cgagctcaga tccggctgct 420

361 ttttggacat aaaagtccct taactaggga agtggttccc gctcgagtct aggccgacga 420

TEV .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aacaaagccc gaaaggaagc tgagttggct gctgccaccg ctgagcaata actagcataa 480

421 ttgtttcggg ctttccttcg actcaaccga cgacggtggc gactcgttat tgatcgtatt 480

.......... .......... T7_term ....... T7_Terminal_pri .......

.......... .......... .......... .......... .......... ..........

481 ccccttgggg cctctaaacg ggtcttgagg ggttttttgc tgaaaggagg aactatatcc 540

481 ggggaacccc ggagatttgc ccagaactcc ccaaaaaacg actttcctcc ttgatatagg 540

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggatatcccg caagaggccc ggcagtaccg gcataaccaa gcctatgcct acagcatcca 600

541 cctatagggc gttctccggg ccgtcatggc cgtattggtt cggatacgga tgtcgtaggt 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gggtgacggt gccgaggatg acgatgagcg cattgttaga tttcatacac ggtgcctgac 660

601 cccactgcca cggctcctac tgctactcgc gtaacaatct aaagtatgtg ccacggactg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... NheI(700) .......... ..........

661 tgcgttagca atttaactgt gataaactac cgcattaaag ctagcttatc gatgataagc 720

661 acgcaatcgt taaattgaca ctatttgatg gcgtaatttc gatcgaatag ctactattcg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tgtcaaacat gagaattaat tcttgaagac gaaagggcct cgtgatacgc ctatttttat 780

721 acagtttgta ctcttaatta agaacttctg ctttcccgga gcactatgcg gataaaaata 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... AatII(816) .......... ..........

781 aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg 840

781 tccaattaca gtactattat taccaaagaa tctgcagtcc accgtgaaaa gcccctttac 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga 900

841 acgcgccttg gggataaaca aataaaaaga tttatgtaag tttatacata ggcgagtact 900

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

901 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac 960

901 ctgttattgg gactatttac gaagttatta taactttttc cttctcatac tcataagttg 960

.......... .......... .......... .......... ....... ORF 3 Ampicillin

.......... .......... .......... .......... .......... ..........

961 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc 1020

961 taaaggcaca gcgggaataa gggaaaaaac gccgtaaaac ggaaggacaa aaacgagtgg 1020

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca 1080

1021 gtctttgcga ccactttcat tttctacgac ttctagtcaa cccacgtgct cacccaatgt 1080

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc 1140

1081 agcttgacct agagttgtcg ccattctagg aactctcaaa agcggggctt cttgcaaaag 1140

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg 1200

1141 gttactactc gtgaaaattt caagacgata caccgcgcca taatagggca caactgcggc 1200

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac 1260

1201 ccgttctcgt tgagccagcg gcgtatgtga taagagtctt actgaaccaa ctcatgagtg 1260

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca 1320

1261 gtcagtgtct tttcgtagaa tgcctaccgt actgtcattc tcttaatacg tcacgacggt 1320

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg 1380

1321 attggtactc actattgtga cgccggttga atgaagactg ttgctagcct cctggcttcc 1380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac 1440

1381 tcgattggcg aaaaaacgtg ttgtaccccc tagtacattg agcggaacta gcaacccttg 1440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... PstI(1493)

1441 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gcagcaatgg 1500

1441 gcctcgactt acttcggtat ggtttgctgc tcgcactgtg gtgctacgga cgtcgttacc 1500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat 1560

1501 gttgttgcaa cgcgtttgat aattgaccgc ttgatgaatg agatcgaagg gccgttgtta 1560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg 1620

1561 attatctgac ctacctccgc ctatttcaac gtcctggtga agacgcgagc cgggaaggcc 1620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg 1680

1621 gaccgaccaa ataacgacta tttagacctc ggccactcgc acccagagcg ccatagtaac 1680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc 1740

1681 gtcgtgaccc cggtctacca ttcgggaggg catagcatca atagatgtgc tgcccctcag 1740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc 1800

1741 tccgttgata cctacttgct ttatctgtct agcgactcta tccacggagt gactaattcg 1800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt 1860

1801 taaccattga cagtctggtt caaatgagta tatatgaaat ctaactaaat tttgaagtaa 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt 1920

1861 aaattaaatt ttcctagatc cacttctagg aaaaactatt agagtactgg ttttagggaa 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt 1980

1921 ttgcactcaa aagcaaggtg actcgcagtc tggggcatct tttctagttt cctagaagaa 1980

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1981 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag 2040

1981 ctctaggaaa aaaagacgcg cattagacga cgaacgtttg tttttttggt ggcgatggtc 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca 2100

2041 gccaccaaac aaacggccta gttctcgatg gttgagaaaa aggcttccat tgaccgaagt 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc caccacttca 2160

2101 cgtctcgcgt ctatggttta tgacaggaag atcacatcgg catcaatccg gtggtgaagt 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg 2220

2161 tcttgagaca tcgtggcgga tgtatggagc gagacgatta ggacaatggt caccgacgac 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg 2280

2221 ggtcaccgct attcagcaca gaatggccca acctgagttc tgctatcaat ggcctattcc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct 2340

2281 gcgtcgccag cccgacttgc cccccaagca cgtgtgtcgg gtcgaacctc gcttgctgga 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga 2400

2341 tgtggcttga ctctatggat gtcgcactcg atactctttc gcggtgcgaa gggcttccct 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc 2460

2401 ctttccgcct gtccataggc cattcgccgt cccagccttg tcctctcgcg tgctccctcg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg 2520

2461 aaggtccccc tttgcggacc atagaaatat caggacagcc caaagcggtg gagactgaac 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg 2580

2521 tcgcagctaa aaacactacg agcagtcccc ccgcctcgga tacctttttg cggtcgttgc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt 2640

2581 gccggaaaaa tgccaaggac cggaaaacga ccggaaaacg agtgtacaag aaaggacgca 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc 2700

2641 ataggggact aagacaccta ttggcataat ggcggaaact cactcgacta tggcgagcgg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcctgatgc 2760

2701 cgtcggcttg ctggctcgcg tcgctcagtc actcgctcct tcgccttctc gcggactacg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ggtattttct ccttacgcat ctgtgcggta tttcacaccg catatatggt gcactctcag 2820

2761 ccataaaaga ggaatgcgta gacacgccat aaagtgtggc gtatatacca cgtgagagtc 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc gctacgtgac 2880

2821 atgttagacg agactacggc gtatcaattc ggtcatatgt gaggcgatag cgatgcactg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg acgggcttgt 2940

2881 acccagtacc gacgcggggc tgtgggcggt tgtgggcgac tgcgcgggac tgcccgaaca 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag 3000

2941 gacgagggcc gtaggcgaat gtctgttcga cactggcaga ggccctcgac gtacacagtc 3000

.......... .......... .......... .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

3001 aggttttcac cgtcatcacc gaaacgcgcg aggcagctgc ggtaaagctc atcagcgtgg 3060

3001 tccaaaagtg gcagtagtgg ctttgcgcgc tccgtcgacg ccatttcgag tagtcgcacc 3060

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tcgtgaagcg attcacagat gtctgcctgt tcatccgcgt ccagctcgtt gagtttctcc 3120

3061 agcacttcgc taagtgtcta cagacggaca agtaggcgca ggtcgagcaa ctcaaagagg 3120

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agaagcgtta atgtctggct tctgataaag cgggccatgt taagggcggt tttttcctgt 3180

3121 tcttcgcaat tacagaccga agactatttc gcccggtaca attcccgcca aaaaaggaca 3180

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttggtcactg atgcctccgt gtaaggggga tttctgttca tgggggtaat gataccgatg 3240

3181 aaccagtgac tacggaggca cattccccct aaagacaagt acccccatta ctatggctac 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aaacgagaga ggatgctcac gatacgggtt actgatgatg aacatgcccg gttactggaa 3300

3241 tttgctctct cctacgagtg ctatgcccaa tgactactac ttgtacgggc caatgacctt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgttgtgagg gtaaacaact ggcggtatgg atgcggcggg accagagaaa aatcactcag 3360

3301 gcaacactcc catttgttga ccgccatacc tacgccgccc tggtctcttt ttagtgagtc 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggtcaatgcc agcgcttcgt taatacagat gtaggtgttc cacagggtag ccagcagcat 3420

3361 ccagttacgg tcgcgaagca attatgtcta catccacaag gtgtcccatc ggtcgtcgta 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cctgcgatgc agatccggaa cataatggtg cagggcgctg acttccgcgt ttccagactt 3480

3421 ggacgctacg tctaggcctt gtattaccac gtcccgcgac tgaaggcgca aaggtctgaa 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tacgaaacac ggaaaccgaa gaccattcat gttgttgctc aggtcgcaga cgttttgcag 3540

3481 atgctttgtg cctttggctt ctggtaagta caacaacgag tccagcgtct gcaaaacgtc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cagcagtcgc ttcacgttcg ctcgcgtatc ggtgattcat tctgctaacc agtaaggcaa 3600

3541 gtcgtcagcg aagtgcaagc gagcgcatag ccactaagta agacgattgg tcattccgtt 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... MscI(3656)

3601 ccccgccagc ctagccgggt cctcaacgac aggagcacga tcatgcgcac ccgtggccag 3660

3601 ggggcggtcg gatcggccca ggagttgctg tcctcgtgct agtacgcgtg ggcaccggtc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gacccaacgc tgcccgagat gcgccgcgtg cggctgctgg agatggcgga cgcgatggat 3720

3661 ctgggttgcg acgggctcta cgcggcgcac gccgacgacc tctaccgcct gcgctaccta 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 atgttctgcc aagggttggt ttgcgcattc acagttctcc gcaagaattg attggctcca 3780

3721 tacaagacgg ttcccaacca aacgcgtaag tgtcaagagg cgttcttaac taaccgaggt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 attcttggag tggtgaatcc gttagcgagg tgccgccggc ttccattcag gtcgaggtgg 3840

3781 taagaacctc accacttagg caatcgctcc acggcggccg aaggtaagtc cagctccacc 3840

.......... .......... .......... .......... ....... tet(0-612) ORF 2

.......... .......... .......... .......... .......... ..........

3841 cccggctcca tgcaccgcga cgcaacgcgg ggaggcagac aaggtatagg gcggcgccta 3900

3841 gggccgaggt acgtggcgct gcgttgcgcc cctccgtctg ttccatatcc cgccgcggat 3900

.......... ....... tet(0-612) ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 caatccatgc caacccgttc catgtgctcg ccgaggcggc ataaatcgcc gtgacgatca 3960

3901 gttaggtacg gttgggcaag gtacacgagc ggctccgccg tatttagcgg cactgctagt 3960

.......... ....... tet(0-612) ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcggtccagt gatcgaagtt aggctggtaa gagccgcgag cgatccttga agctgtccct 4020

3961 cgccaggtca ctagcttcaa tccgaccatt ctcggcgctc gctaggaact tcgacaggga 4020

.......... ....... tet(0-612) ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gatggtcgtc atctacctgc ctggacagca tggcctgcaa cgcgggcatc ccgatgccgc 4080

4021 ctaccagcag tagatggacg gacctgtcgt accggacgtt gcgcccgtag ggctacggcg 4080

.......... ....... tet(0-612) ORF 2 ORF 2 ....... ..........

.......... .......... .......... .......... .......... ..........

4081 cggaagcgag aagaatcata atggggaagg ccatccagcc tcgcgtcgcg aacgccagca 4140

4081 gccttcgctc ttcttagtat taccccttcc ggtaggtcgg agcgcagcgc ttgcggtcgt 4140

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... EagI(4159) .......... .......... .......... ..........

4141 agacgtagcc cagcgcgtcg gccgccatgc cggcgataat ggcctgcttc tcgccgaaac 4200

4141 tctgcatcgg gtcgcgcagc cggcggtacg gccgctatta ccggacgaag agcggctttg 4200

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gtttggtggc gggaccagtg acgaaggctt gagcgagggc gtgcaagatt ccgaataccg 4260

4201 caaaccaccg ccctggtcac tgcttccgaa ctcgctcccg cacgttctaa ggcttatggc 4260

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 caagcgacag gccgatcatc gtcgcgctcc agcgaaagcg gtcctcgccg aaaatgaccc 4320

4261 gttcgctgtc cggctagtag cagcgcgagg tcgctttcgc caggagcggc ttttactggg 4320

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 agagcgctgc cggcacctgt cctacgagtt gcatgataaa gaagacagtc ataagtgcgg 4380

4321 tctcgcgacg gccgtggaca ggatgctcaa cgtactattt cttctgtcag tattcacgcc 4380

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgacgatagt catgccccgc gcccaccgga aggagctgac tgggttgaag gctctcaagg 4440

4381 gctgctatca gtacggggcg cgggtggcct tcctcgactg acccaacttc cgagagttcc 4440

.......... .... tet(0-612) ORF 2 ORF 2 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gcatcggtcg agatcccggt gcctaatgag tgagctaact tacattaatt gcgttgcgct 4500

4441 cgtagccagc tctagggcca cggattactc actcgattga atgtaattaa cgcaacgcga 4500

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac 4560

4501 gtgacgggcg aaaggtcagc cctttggaca gcacggtcga cgtaattact tagccggttg 4560

.......... ....... ORF 2 ORF 3 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gcgcggggag aggcggtttg cgtattgggc gccagggtgg tttttctttt caccagtgag 4620

4561 cgcgcccctc tccgccaaac gcataacccg cggtcccacc aaaaagaaaa gtggtcactc 4620

.......... ....... ORF 2 ORF 3 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acgggcaaca gctgattgcc cttcaccgcc tggccctgag agagttgcag caagcggtcc 4680

4621 tgcccgttgt cgactaacgg gaagtggcgg accgggactc tctcaacgtc gttcgccagg 4680

.......... ....... ORF 2 ORF 3 lacI ....... .......... ..........

.......... .......... .......... .......... HpaI(4725) ..........

4681 acgctggttt gccccagcag gcgaaaatcc tgtttgatgg tggttaacgg cgggatataa 4740

4681 tgcgaccaaa cggggtcgtc cgcttttagg acaaactacc accaattgcc gccctatatt 4740

.......... ORF 2 ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 catgagctgt cttcggtatc gtcgtatccc actaccgaga tatccgcacc aacgcgcagc 4800

4741 gtactcgaca gaagccatag cagcataggg tgatggctct ataggcgtgg ttgcgcgtcg 4800

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ccggactcgg taatggcgcg cattgcgccc agcgccatct gatcgttggc aaccagcatc 4860

4801 ggcctgagcc attaccgcgc gtaacgcggg tcgcggtaga ctagcaaccg ttggtcgtag 4860

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gcagtgggaa cgatgccctc attcagcatt tgcatggttt gttgaaaacc ggacatggca 4920

4861 cgtcaccctt gctacgggag taagtcgtaa acgtaccaaa caacttttgg cctgtaccgt 4920

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ctccagtcgc cttcccgttc cgctatcggc tgaatttgat tgcgagtgag atatttatgc 4980

4921 gaggtcagcg gaagggcaag gcgatagccg acttaaacta acgctcactc tataaatacg 4980

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... ApaI(5024) ..........

4981 cagccagcca gacgcagacg cgccgagaca gaacttaatg ggcccgctaa cagcgcgatt 5040

4981 gtcggtcggt ctgcgtctgc gcggctctgt cttgaattac ccgggcgatt gtcgcgctaa 5040

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tgctggtgac ccaatgcgac cagatgctcc acgcccagtc gcgtaccgtc ttcatgggag 5100

5041 acgaccactg ggttacgctg gtctacgagg tgcgggtcag cgcatggcag aagtaccctc 5100

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aaaataatac tgttgatggg tgtctggtca gagacatcaa gaaataacgc cggaacatta 5160

5101 ttttattatg acaactaccc acagaccagt ctctgtagtt ctttattgcg gccttgtaat 5160

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... BclI(5213)

5161 gtgcaggcag cttccacagc aatggcatcc tggtcatcca gcggatagtt aatgatcagc 5220

5161 cacgtccgtc gaaggtgtcg ttaccgtagg accagtaggt cgcctatcaa ttactagtcg 5220

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ccactgacgc gttgcgcgag aagattgtgc accgccgctt tacaggcttc gacgccgctt 5280

5221 ggtgactgcg caacgcgctc ttctaacacg tggcggcgaa atgtccgaag ctgcggcgaa 5280

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cgttctacca tcgacaccac cacgctggca cccagttgat cggcgcgaga tttaatcgcc 5340

5281 gcaagatggt agctgtggtg gtgcgaccgt gggtcaacta gccgcgctct aaattagcgg 5340

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gcgacaattt gcgacggcgc gtgcagggcc agactggagg tggcaacgcc aatcagcaac 5400

5341 cgctgttaaa cgctgccgcg cacgtcccgg tctgacctcc accgttgcgg ttagtcgttg 5400

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gactgtttgc ccgccagttg ttgtgccacg cggttgggaa tgtaattcag ctccgccatc 5460

5401 ctgacaaacg ggcggtcaac aacacggtgc gccaaccctt acattaagtc gaggcggtag 5460

.......... .......... ORF 3 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gccgcttcca ctttttcccg cgttttcgca gaaacgtggc tggcctggtt caccacgcgg 5520

5461 cggcgaaggt gaaaaagggc gcaaaagcgt ctttgcaccg accggaccaa gtggtgcgcc 5520

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 gaaacggtct gataagagac accggcatac tctgcgacat cgtataacgt tactggtttc 5580

5521 ctttgccaga ctattctctg tggccgtatg agacgctgta gcatattgca atgaccaaag 5580

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 acattcacca ccctgaattg actctcttcc gggcgctatc atgccatacc gcgaaaggtt 5640

5581 tgtaagtggt gggacttaac tgagagaagg cccgcgatag tacggtatgg cgctttccaa 5640

lacI .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ttgcgccatt cgatggtgtc cgggatctcg acgctctccc ttatgcgact cctgcattag 5700

5641 aacgcggtaa gctaccacag gccctagagc tgcgagaggg aatacgctga ggacgtaatc 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gaagcagccc agtagtaggt tgaggccgtt gagcaccgcc gccgcaagga atggtgcatg 5760

5701 cttcgtcggg tcatcatcca actccggcaa ctcgtggcgg cggcgttcct taccacgtac 5760

.......... .......... .......... .......... .......... ..........