Sequence of pET200_Directional

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Sequence of pET200_Directional_TOPO

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Sequence of pET200 Directional TOPO : 5741 BP; 1289 A; 1551 C; 1610 G; 1291 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caaggagatg gcgcccaaca gtcccccggc cacggggcct gccaccatac ccacgccgaa 60

1 gttcctctac cgcgggttgt cagggggccg gtgccccgga cggtggtatg ggtgcggctt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 acaagcgctc atgagcccga agtggcgagc ccgatcttcc ccatcggtga tgtcggcgat 120

61 tgttcgcgag tactcgggct tcaccgctcg ggctagaagg ggtagccact acagccgcta 120

.......... .......... .......... .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

121 ataggcgcca gcaaccgcac ctgtggcgcc ggtgatgccg gccacgatgc gtccggcgta 180

121 tatccgcggt cgttggcgtg gacaccgcgg ccactacggc cggtgctacg caggccgcat 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gaggatcgag atctcgatcc cgcgaaatta atacgactca ctatagggga attgtgagcg 240

181 ctcctagctc tagagctagg gcgctttaat tatgctgagt gatatcccct taacactcgc 240

.......... .......... .......... T7_pro .......... lacO

.......... XbaI(255) .......... .......... .......... ..........

241 gataacaatt cccctctaga aataattttg tttaacttta agaaggagat atacatatgc 300

241 ctattgttaa ggggagatct ttattaaaac aaattgaaat tcttcctcta tatgtatacg 300

lacO .......... ....... T7_transl_en_RBS ....... ..........

.......... .......... .......... NheI(333) .......... ..........

301 ggggttctca tcatcatcat catcatggta tggctagcat gactggtgga cagcaaatgg 360

301 ccccaagagt agtagtagta gtagtaccat accgatcgta ctgaccacct gtcgtttacc 360

.......... 6xHis Xpress_fwd_pri T7_gene10_leader T7_leader

.......... .......... .......... .......... .......... SacI(415)

361 gtcgggatct gtacgacgat gacgataagg atcatccctt caccaagggc gagctcaacg 420

361 cagccctaga catgctgcta ctgctattcc tagtagggaa gtggttcccg ctcgagttgc 420

.......... Xpress_EK .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat 480

421 taggccgacg attgtttcgg gctttccttc gactcaaccg acgacggtgg cgactcgtta 480

.......... .......... .......... T7_term ....... T7_Terminal_pri

.......... .......... .......... .......... .......... ..........

481 aactagcata accccttggg gcctctaaac gggtcttgag gagttttttg ctgaaaggag 540

481 ttgatcgtat tggggaaccc cggagatttg cccagaactc ctcaaaaaac gactttcctc 540

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gaactatatc cggatatccc gcaagaggcc cggcagtacc ggcataacca agcctatgcc 600

541 cttgatatag gcctataggg cgttctccgg gccgtcatgg ccgtattggt tcggatacgg 600

T7_term .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tacagcatcc agggtgacgg tgccgaggat gacgatgagc gcattgttag atttcataca 660

601 atgtcgtagg tcccactgcc acggctccta ctgctactcg cgtaacaatc taaagtatgt 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... HindIII(709) .........

661 cggtgcctga ctgcgttagc aatttaactg tgataaacta ccgcattaaa gcttatcgat 720

661 gccacggact gacgcaatcg ttaaattgac actatttgat ggcgtaattt cgaatagcta 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gataagctgt caaacatgag aattaattct tgaagacgaa agggcctcgt gatacgccta 780

721 ctattcgaca gtttgtactc ttaattaaga acttctgctt tcccggagca ctatgcggat 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... AatII(823) ..........

781 tttttatagg ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg 840

781 aaaaatatcc aattacagta ctattattac caaagaatct gcagtccacc gtgaaaagcc 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg 900

841 cctttacacg cgccttgggg ataaacaaat aaaaagattt atgtaagttt atacataggc 900

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

901 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgatt 960

901 gagtactctg ttattgggac tatttacgaa gttattataa ctttttcctt ctcatactaa 960

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gaacaagatg gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat 1020

961 cttgttctac ctaacgtgcg tccaagaggc cggcgaaccc acctctccga taagccgata 1020

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gactgggcac aactgacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag 1080

1021 ctgacccgtg ttgactgtta gccgacgaga ctacggcggc acaaggccga cagtcgcgtc 1080

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... PstI(1136)

1081 gggcgcccgg ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac 1140

1081 cccgcgggcc aagaaaaaca gttctggctg gacaggccac gggacttact tgacgtcctg 1140

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gaggcagcgc ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac 1200

1141 ctccgtcgcg ccgatagcac cgaccggtgc tgcccgcaag gaacgcgtcg acacgagctg 1200

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gttgtcactg aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc 1260

1201 caacagtgac ttcgcccttc cctgaccgac gataacccgc ttcacggccc cgtcctagag 1260

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctgtcatctc accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg 1320

1261 gacagtagag tggaacgagg acggctcttt cataggtagt accgactacg ttacgccgcc 1320

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctgcatacgc ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag 1380

1321 gacgtatgcg aactaggccg atggacgggt aagctggtgg ttcgctttgt agcgtagctc 1380

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cgggcacgta ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat 1440

1381 gcccgtgcat gagcctacct tcggccagaa cagctagtcc tactagacct gcttctcgta 1440

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 caggggctcg cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag 1500

1441 gtccccgagc gcggtcggct tgacaagcgg tccgagttcc gcgcgtacgg gctgccgctc 1500

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gatctcgtcg tgacacatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc 1560

1501 ctagagcagc actgtgtacc gctacggacg aacggcttat agtaccacct tttaccggcg 1560

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ttttctggat tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg 1620

1561 aaaagaccta agtagctgac accggccgac ccacaccgcc tggcgatagt cctgtatcgc 1620

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ttggctaccc gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg 1680

1621 aaccgatggg cactataacg acttctcgaa ccgccgctta cccgactggc gaaggagcac 1680

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ctttacggta tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag 1740

1681 gaaatgccat agcggcgagg gctaagcgtc gcgtagcgga agatagcgga agaactgctc 1740

.......... ... ORF 1 Neomycin(variant) ... .......... ..........

.......... ......... BstBI(1765) ......... .......... ..........

1741 ttcttctgag cgggactctg gggttcgaaa tgaccgacca agcgacgcct aactgtcaga 1800

1741 aagaagactc gccctgagac cccaagcttt actggctggt tcgctgcgga ttgacagtct 1800

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat 1860

1801 ggttcaaatg agtatatatg aaatctaact aaattttgaa gtaaaaatta aattttccta 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt 1920

1861 gatccacttc taggaaaaac tattagagta ctggttttag ggaattgcac tcaaaagcaa 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct 1980

1921 ggtgactcgc agtctggggc atcttttcta gtttcctaga agaactctag gaaaaaaaga 1980

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1981 gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc 2040

1981 cgcgcattag acgacgaacg tttgtttttt tggtggcgat ggtcgccacc aaacaaacgg 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc 2100

2041 cctagttctc gatggttgag aaaaaggctt ccattgaccg aagtcgtctc gcgtctatgg 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc 2160

2101 tttatgacag gaagatcaca tcggcatcaa tccggtggtg aagttcttga gacatcgtgg 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc 2220

2161 cggatgtatg gagcgagacg attaggacaa tggtcaccga cgacggtcac cgctattcag 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg 2280

2221 cacagaatgg cccaacctga gttctgctat caatggccta ttccgcgtcg ccagcccgac 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata 2340

2281 ttgcccccca agcacgtgtg tcgggtcgaa cctcgcttgc tggatgtggc ttgactctat 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta 2400

2341 ggatgtcgca ctcgatactc tttcgcggtg cgaagggctt ccctctttcc gcctgtccat 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc 2460

2401 aggccattcg ccgtcccagc cttgtcctct cgcgtgctcc ctcgaaggtc cccctttgcg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg 2520

2461 gaccatagaa atatcaggac agcccaaagc ggtggagact gaactcgcag ctaaaaacac 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt 2580

2521 tacgagcagt ccccccgcct cggatacctt tttgcggtcg ttgcgccgga aaaatgccaa 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt 2640

2581 ggaccggaaa acgaccggaa aacgagtgta caagaaagga cgcaataggg gactaagaca 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga 2700

2641 cctattggca taatggcgga aactcactcg actatggcga gcggcgtcgg cttgctggct 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcgcagcgag tcagtgagcg aggaagcgga agagcgcctg atgcggtatt ttctccttac 2760

2701 cgcgtcgctc agtcactcgc tccttcgcct tctcgcggac tacgccataa aagaggaatg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcatctgtgc ggtatttcac accgcaatgg tgcactctca gtacaatctg ctctgatgcc 2820

2761 cgtagacacg ccataaagtg tggcgttacc acgtgagagt catgttagac gagactacgg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gcatagttaa gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc 2880

2821 cgtatcaatt cggtcatatg tgaggcgata gcgatgcact gacccagtac cgacgcgggg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 2940

2881 ctgtgggcgg ttgtgggcga ctgcgcggga ctgcccgaac agacgagggc cgtaggcgaa 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 3000

2941 tgtctgttcg acactggcag aggccctcga cgtacacagt ctccaaaagt ggcagtagtg 3000

.......... .......... .......... pGEX_3_pri ROP ..........

.......... .......... .......... .......... .......... ..........

3001 cgaaacgcgc gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga 3060

3001 gctttgcgcg ctccgtcgac gccatttcga gtagtcgcac cagcacttcg ctaagtgtct 3060

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgtctgcctg ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc 3120

3061 acagacggac aagtaggcgc aggtcgagca actcaaagag gtcttcgcaa ttacagaccg 3120

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttctgataaa gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg 3180

3121 aagactattt cgcccggtac aattcccgcc aaaaaaggac aaaccagtga ctacggaggc 3180

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tgtaaggggg atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca 3240

3181 acattccccc taaagacaag tacccccatt actatggcta ctttgctctc tcctacgagt 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cgatacgggt tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac 3300

3241 gctatgccca atgactacta cttgtacggg ccaatgacct tgcaacactc ccatttgttg 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tggcggtatg gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg 3360

3301 accgccatac ctacgccgcc ctggtctctt tttagtgagt cccagttacg gtcgcgaagc 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ttaatacaga tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga 3420

3361 aattatgtct acatccacaa ggtgtcccat cggtcgtcgt aggacgctac gtctaggcct 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acataatggt gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga 3480

3421 tgtattacca cgtcccgcga ctgaaggcgc aaaggtctga aatgctttgt gcctttggct 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agaccattca tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc 3540

3481 tctggtaagt acaacaacga gtccagcgtc tgcaaaacgt cgtcgtcagc gaagtgcaag 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gctcgcgtat cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg 3600

3541 cgagcgcata gccactaagt aagacgattg gtcattccgt tggggcggtc ggatcggccc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcctcaacga caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgcccgaga 3660

3601 aggagttgct gtcctcgtgc tagtacgcgt gggcaccggt cctgggttgc gacgggctct 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tgcgccgcgt gcggctgctg gagatggcgg acgcgatgga tatgttctgc caagggttgg 3720

3661 acgcggcgca cgccgacgac ctctaccgcc tgcgctacct atacaagacg gttcccaacc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tttgcgcatt cacagttctc cgcaagaatt gattggctcc aattcttgga gtggtgaatc 3780

3721 aaacgcgtaa gtgtcaagag gcgttcttaa ctaaccgagg ttaagaacct caccacttag 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cgttagcgag gtgccgccgg cttccattca ggtcgaggtg gcccggctcc atgcaccgcg 3840

3781 gcaatcgctc cacggcggcc gaaggtaagt ccagctccac cgggccgagg tacgtggcgc 3840

.......... .......... .......... ....... tet(0-612) ORF 1 .......

.......... .......... .......... .......... .......... ..........

3841 acgcaacgcg gggaggcaga caaggtatag ggcggcgcct acaatccatg ccaacccgtt 3900

3841 tgcgttgcgc ccctccgtct gttccatatc ccgccgcgga tgttaggtac ggttgggcaa 3900

.......... ....... tet(0-612) ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc agcggtccaa tgatcgaagt 3960

3901 ggtacacgag cggctccgcc gtatttagcg gcactgctag tcgccaggtt actagcttca 3960

.......... ....... tet(0-612) ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 taggctggta agagccgcga gcgatccttg aagctgtccc tgatggtcgt catctacctg 4020

3961 atccgaccat tctcggcgct cgctaggaac ttcgacaggg actaccagca gtagatggac 4020

.......... ....... tet(0-612) ORF 1 ....... .......... ORF 1

.......... .......... .......... .......... .......... ..........

4021 cctggacagc atggcctgca acgcgggcat cccgatgccg ccggaagcga gaagaatcat 4080

4021 ggacctgtcg taccggacgt tgcgcccgta gggctacggc ggccttcgct cttcttagta 4080

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... .......... NruI(4109) .......... .......... ..........

4081 aatggggaag gccatccagc ctcgcgtcgc gaacgccagc aagacgtagc ccagcgcgtc 4140

4081 ttaccccttc cggtaggtcg gagcgcagcg cttgcggtcg ttctgcatcg ggtcgcgcag 4140

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa cgtttggtgg cgggaccagt 4200

4141 ccggcggtac ggccgctatt accggacgaa gagcggcttt gcaaaccacc gccctggtca 4200

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc gcaagcgaca ggccgatcat 4260

4201 ctgcttccga actcgctccc gcacgttcta aggcttatgg cgttcgctgt ccggctagta 4260

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc cagagcgctg ccggcacctg 4320

4261 gcagcgcgag gtcgctttcg ccaggagcgg cttttactgg gtctcgcgac ggccgtggac 4320

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tcctacgagt tgcatgataa agaagacagt cataagtgcg gcgacgatag tcatgccccg 4380

4321 aggatgctca acgtactatt tcttctgtca gtattcacgc cgctgctatc agtacggggc 4380

.......... .... tet(0-612) ORF 1 ORF 1 .... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgcccaccgg aaggagctga ctgggttgaa ggctctcaag ggcatcggtc gagatcccgg 4440

4381 gcgggtggcc ttcctcgact gacccaactt ccgagagttc ccgtagccag ctctagggcc 4440

......... tet(0-612) ORF 1 ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tgcctaatga gtgagctaac ttacattaat tgcgttgcgc tcactgcccg ctttccagtc 4500

4441 acggattact cactcgattg aatgtaatta acgcaacgcg agtgacgggc gaaaggtcag 4500

.......... .......... ORF 1 .......... ORF 2 lacI ..........

.......... .......... .......... .......... .......... ..........

4501 gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt 4560

4501 ccctttggac agcacggtcg acgtaattac ttagccggtt gcgcgcccct ctccgccaaa 4560

.......... ....... ORF 1 ORF 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gcgtattggg cgccagggtg gtttttcttt tcaccagtga gacgggcaac agctgattgc 4620

4561 cgcataaccc gcggtcccac caaaaagaaa agtggtcact ctgcccgttg tcgactaacg 4620

.......... ....... ORF 1 ORF 2 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ccttcaccgc ctggccctga gagagttgca gcaagcggtc cacgctggtt tgccccagca 4680

4621 ggaagtggcg gaccgggact ctctcaacgt cgttcgccag gtgcgaccaa acggggtcgt 4680

.......... ....... ORF 1 ORF 2 lacI ....... .......... ..........

.......... .......... HpaI(4706) .......... .......... ..........

4681 ggcgaaaatc ctgtttgatg gtggttaacg gcgggatata acatgagctg tcttcggtat 4740

4681 ccgcttttag gacaaactac caccaattgc cgccctatat tgtactcgac agaagccata 4740

ORF 1 .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 cgtcgtatcc cactaccgag atatccgcac caacgcgcag cccggactcg gtaatggcgc 4800

4741 gcagcatagg gtgatggctc tataggcgtg gttgcgcgtc gggcctgagc cattaccgcg 4800

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gcattgcgcc cagcgccatc tgatcgttgg caaccagcat cgcagtggga acgatgccct 4860

4801 cgtaacgcgg gtcgcggtag actagcaacc gttggtcgta gcgtcaccct tgctacggga 4860

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 cattcagcat ttgcatggtt tgttgaaaac cggacatggc actccagtcg ccttcccgtt 4920

4861 gtaagtcgta aacgtaccaa acaacttttg gcctgtaccg tgaggtcagc ggaagggcaa 4920

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ccgctatcgg ctgaatttga ttgcgagtga gatatttatg ccagccagcc agacgcagac 4980

4921 ggcgatagcc gacttaaact aacgctcact ctataaatac ggtcggtcgg tctgcgtctg 4980

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... ApaI(5005) .......... .......... ..........

4981 gcgccgagac agaacttaat gggcccgcta acagcgcgat ttgctggtga cccaatgcga 5040

4981 cgcggctctg tcttgaatta cccgggcgat tgtcgcgcta aacgaccact gggttacgct 5040

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ccagatgctc cacgcccagt cgcgtaccgt cttcatggga gaaaataata ctgttgatgg 5100

5041 ggtctacgag gtgcgggtca gcgcatggca gaagtaccct cttttattat gacaactacc 5100

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gtgtctggtc agagacatca agaaataacg ccggaacatt agtgcaggca gcttccacag 5160

5101 cacagaccag tctctgtagt tctttattgc ggccttgtaa tcacgtccgt cgaaggtgtc 5160

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... BclI(5194) .......... ..........

5161 caatggcatc ctggtcatcc agcggatagt taatgatcag cccactgacg cgttgcgcga 5220

5161 gttaccgtag gaccagtagg tcgcctatca attactagtc gggtgactgc gcaacgcgct 5220

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gaagattgtg caccgccgct ttacaggctt cgacgccgct tcgttctacc atcgacacca 5280

5221 cttctaacac gtggcggcga aatgtccgaa gctgcggcga agcaagatgg tagctgtggt 5280

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ccacgctggc acccagttga tcggcgcgag atttaatcgc cgcgacaatt tgcgacggcg 5340

5281 ggtgcgaccg tgggtcaact agccgcgctc taaattagcg gcgctgttaa acgctgccgc 5340

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cgtgcagggc cagactggag gtggcaacgc caatcagcaa cgactgtttg cccgccagtt 5400

5341 gcacgtcccg gtctgacctc caccgttgcg gttagtcgtt gctgacaaac gggcggtcaa 5400

.......... .......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gttgtgccac gcggttggga atgtaattca gctccgccat cgccgcttcc actttttccc 5460

5401 caacacggtg cgccaaccct tacattaagt cgaggcggta gcggcgaagg tgaaaaaggg 5460

.......... ORF 2 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gcgttttcgc agaaacgtgg ctggcctggt tcaccacgcg ggaaacggtc tgataagaga 5520

5461 cgcaaaagcg tctttgcacc gaccggacca agtggtgcgc cctttgccag actattctct 5520

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 caccggcata ctctgcgaca tcgtataacg ttactggttt cacattcacc accctgaatt 5580

5521 gtggccgtat gagacgctgt agcatattgc aatgaccaaa gtgtaagtgg tgggacttaa 5580

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 gactctcttc cgggcgctat catgccatac cgcgaaaggt tttgcgccat tcgatggtgt 5640

5581 ctgagagaag gcccgcgata gtacggtatg gcgctttcca aaacgcggta agctaccaca 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ccgggatctc gacgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg 5700

5641 ggccctagag ctgcgagagg gaatacgctg aggacgtaat ccttcgtcgg gtcatcatcc 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat g......... .......... 5741

5701 aactccggca actcgtggcg gcggcgttcc ttaccacgta c......... .......... 5741

.......... .......... .......... .......... .......... ..........