Sequence of pET28a

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Sequence of pET28a

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Sequence of pET28a : 5369 BP; 1268 A; 1391 C; 1444 G; 1266 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 atccggatat agttcctcct ttcagcaaaa aacccctcaa gacccgttta gaggccccaa 60

1 taggcctata tcaaggagga aagtcgtttt ttggggagtt ctgggcaaat ctccggggtt 60

.......... .......... T7_t .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ggggttatgc tagttattgc tcagcggtgg cagcagccaa ctcagcttcc tttcgggctt 120

61 ccccaatacg atcaataacg agtcgccacc gtcgtcggtt gagtcgaagg aaagcccgaa 120

.......... T7_T_p T7_t .......... .......... ..........

.......... .......... .... XhoI(158) NotI(166),EagI(166) HindIII(173)

121 tgttagcagc cggatctcag tggtggtggt ggtggtgctc gagtgcggcc gcaagcttgt 180

121 acaatcgtcg gcctagagtc accaccacca ccaccacgag ctcacgccgg cgttcgaaca 180

T7_t .......... 6xHis .......... .......... ..........

SacI(190),EcoRI(192) BamHI(198) ........ .......... NheI(231)

181 cgacggagct cgaattcgga tccgcgaccc atttgctgtc caccagtcat gctagccata 240

181 gctgcctcga gcttaagcct aggcgctggg taaacgacag gtggtcagta cgatcggtat 240

.......... .......... .......... ...... T7_g10_l T7_leader X_fwd_pri

.......... .......... .......... .......... .......... ..........

241 tggctgccgc gcggcaccag gccgctgctg tgatgatgat gatgatggct gctgcccatg 300

241 accgacggcg cgccgtggtc cggcgacgac actactacta ctactaccga cgacgggtac 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... XbaI(335) .......... ..........

301 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca 360

301 catatagagg aagaatttca atttgtttta ataaagatct ccccttaaca ataggcgagt 360

.......... T7_t_e_RBS .......... .......... lacO ..........

.......... .......... .......... .......... BglII(401) ..........

361 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc 420

361 gttaagggga tatcactcag cataattaaa gcgccctagc tctagagcta ggagatgcgg 420

.......... T7_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 480

421 cctgcgtagc accggccgta gtggccgcgg tgtccacgcc aacgaccgcg gatatagcgg 480

.......... .......... tet .......... ....... pBRrevBam_primer

.......... .......... .......... .......... .......... ..........

481 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 540

481 ctgtagtggc taccccttct agcccgagcg gtgaagcccg agtactcgcg aacaaagccg 540

pBRrevBam_primer .... tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca 600

541 cacccatacc accgtccggg gcaccggccc cctgacaacc cgcggtagag gaacgtacgt 600

.......... .......... tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg 660

601 ggtaaggaac gccgccgcca cgagttgccg gagttggatg atgacccgac gaaggattac 660

.......... .......... tet .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt 720

661 gtcctcagcg tattccctct cgcagctcta gggcctgtgg tagcttaccg cgttttggaa 720

.......... tet .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc 780

721 agcgccatac cgtactatcg cgggccttct ctcagttaag tcccaccact tacactttgg 780

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

781 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 840

781 tcattgcaat atgctacagc gtctcatacg gccacagaga atagtctggc aaagggcgca 840

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 900

841 ccacttggtc cggtcggtgc aaagacgctt ttgcgccctt tttcaccttc gccgctaccg 900

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 960

901 cctcgactta atgtaagggt tggcgcaccg tgttgttgac cgcccgtttg tcagcaacga 960

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 1020

961 ctaaccgcaa cggtggaggt cagaccggga cgtgcgcggc agcgtttaac agcgccgcta 1020

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 1080

1021 atttagagcg cggctagttg acccacggtc gcaccaccac agctaccatc ttgcttcgcc 1080

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... BclI(1137)

1081 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 1140

1081 gcagcttcgg acatttcgcc gccacgtgtt agaagagcgc gttgcgcagt cacccgacta 1140

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 1200

1141 gtaattgata ggcgacctac tggtcctacg gtaacgacac cttcgacgga cgtgattaca 1200

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 1260

1201 aggccgcaat aaagaactac agagactggt ctgtgggtag ttgtcataat aaaagagggt 1260

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1320

1261 acttctgcca tgcgctgacc cgcacctcgt agaccagcgt aacccagtgg tcgtttagcg 1320

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... ApaI(1334) .......... .......... .......... ..........

1321 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1380

1321 cgacaatcgc ccgggtaatt caagacagag ccgcgcagac gcagaccgac cgaccgtatt 1380

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1440

1381 tatagagtga gcgttagttt aagtcggcta tcgccttgcc cttccgctga cctcacggta 1440

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1500

1441 caggccaaaa gttgtttggt acgtttacga cttactcccg tagcaagggt gacgctacga 1500

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1560

1501 ccaacggttg ctagtctacc gcgacccgcg ttacgcgcgg taatggctca ggcccgacgc 1560

.......... ....... lacI Orf frame 2 ....... .......... ..........

......... EcoRV(1573) ......... .......... .......... ..........

1561 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1620

1561 gcaaccacgc ctatagagcc atcaccctat gctgctatgg cttctgtcga gtacaatata 1620

.......... ....... lacI Orf frame 2 ....... .......... ..........

HpaI(1629) .......... .......... .......... .......... ..........

1621 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1680

1621 gggcggcaat tggtggtagt ttgtcctaaa agcggacgac cccgtttggt cgcacctggc 1680

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1740

1681 gaacgacgtt gagagagtcc cggtccgcca cttcccgtta gtcgacaacg ggcagagtga 1740

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1800

1741 ccacttttct ttttggtggg accgcgggtt atgcgtttgg cggagagggg cgcgcaaccg 1800

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 1860

1801 gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg tcactcgcgt 1860

.......... ....... lacI Orf frame 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga 1920

1861 tgcgttaatt acattcaatc gagtgagtaa tccgtggccc tagagctggc tacgggaact 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 1980

1921 ctcggaagtt gggtcagtcg aggaaggcca cccgcgcccc gtactgatag cagcggcgtg 1980

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca 2040

1981 aatactgaca gaagaaatag tacgttgagc atcctgtcca cggccgtcgc gagacccagt 2040

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat 2100

2041 aaaagccgct cctggcgaaa gcgacctcgc gctgctacta gccggacagc gaacgccata 2100

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg 2160

2101 agccttagaa cgtgcgggag cgagttcgga agcagtgacc agggcggtgg tttgcaaagc 2160

.......... ......... tet(852-576) ......... .......... ..........

.......... .......... .......... .......... FspI(2205) ..........

2161 gcgagaagca ggccattatc gccggcatgg cggccccacg ggtgcgcatg atcgtgctcc 2220

2161 cgctcttcgt ccggtaatag cggccgtacc gccggggtgc ccacgcgtac tagcacgagg 2220

......... tet(852-576) ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tgtcgttgag gacccggcta ggctggcggg gttgccttac tggttagcag aatgaatcac 2280

2221 acagcaactc ctgggccgat ccgaccgccc caacggaatg accaatcgtc ttacttagtg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgatacgcga gcgaacgtga agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa 2340

2281 gctatgcgct cgcttgcact tcgctgacga cgacgttttg cagacgctgg actcgttgtt 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 catgaatggt cttcggtttc cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct 2400

2341 gtacttacca gaagccaaag gcacaaagca tttcagacct ttgcgccttc agtcgcggga 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcaccattat gttccggatc tgcatcgcag gatgctgctg gctaccctgt ggaacaccta 2460

2401 cgtggtaata caaggcctag acgtagcgtc ctacgacgac cgatgggaca ccttgtggat 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 catctgtatt aacgaagcgc tggcattgac cctgagtgat ttttctctgg tcccgccgca 2520

2461 gtagacataa ttgcttcgcg accgtaactg ggactcacta aaaagagacc agggcggcgt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tccataccgc cagttgttta ccctcacaac gttccagtaa ccgggcatgt tcatcatcag 2580

2521 aggtatggcg gtcaacaaat gggagtgttg caaggtcatt ggcccgtaca agtagtagtc 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 taacccgtat cgtgagcatc ctctctcgtt tcatcggtat cattaccccc atgaacagaa 2640

2581 attgggcata gcactcgtag gagagagcaa agtagccata gtaatggggg tacttgtctt 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atccccctta cacggaggca tcagtgacca aacaggaaaa aaccgccctt aacatggccc 2700

2641 tagggggaat gtgcctccgt agtcactggt ttgtcctttt ttggcgggaa ttgtaccggg 2700

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2701 gctttatcag aagccagaca ttaacgcttc tggagaaact caacgagctg gacgcggatg 2760

2701 cgaaatagtc ttcggtctgt aattgcgaag acctctttga gttgctcgac ctgcgcctac 2760

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aacaggcaga catctgtgaa tcgcttcacg accacgctga tgagctttac cgcagctgcc 2820

2761 ttgtccgtct gtagacactt agcgaagtgc tggtgcgact actcgaaatg gcgtcgacgg 2820

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 2880

2821 agcgcgcaaa gccactactg ccacttttgg agactgtgta cgtcgagggc ctctgccagt 2880

.......... ROP ........ pGEX_3_primer ........ ..........

.......... .......... .......... .......... .......... ..........

2881 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 2940

2881 gtcgaacaga cattcgccta cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttggcgggtg tcggggcgca gccatgaccc agtcacgtag cgatagcgga gtgtatactg 3000

2941 aaccgcccac agccccgcgt cggtactggg tcagtgcatc gctatcgcct cacatatgac 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gcttaactat gcggcatcag agcagattgt actgagagtg caccatatat gcggtgtgaa 3060

3001 cgaattgata cgccgtagtc tcgtctaaca tgactctcac gtggtatata cgccacactt 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc 3120

3061 tatggcgtgt ctacgcattc ctcttttatg gcgtagtccg cgagaaggcg aaggagcgag 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 3180

3121 tgactgagcg acgcgagcca gcaagccgac gccgctcgcc atagtcgagt gagtttccgc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 3240

3181 cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac tcgttttccg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 3300

3241 gtcgttttcc ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt atccgaggcg 3300

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3301 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 3360

3301 gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt gggctgtcct 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 3420

3361 gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg acaaggctgg 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 3480

3421 gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg cgaaagagta 3480

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 3540

3481 tcgagtgcga catccataga gtcaagccac atccagcaag cgaggttcga cccgacacac 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 3600

3541 gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc cattgatagc agaactcagg 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 3660

3601 ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc ctaatcgtct 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 3720

3661 cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat gccgatgtga 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 3780

3721 tcttcctgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc tttttctcaa 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 3840

3781 ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc caccaaaaaa acaaacgttc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 3900

3841 gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag gaaactagaa aagatgcccc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgaa caataaaact 3960

3901 agactgcgag tcaccttgct tttgagtgca attccctaaa accagtactt gttattttga 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtctgcttac ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc 4020

3961 cagacgaatg tatttgtcat tatgttcccc acaatactcg gtataagttg ccctttgcag 4020

.......... .......... .......... ...... Orf frame 2 KanR2 ......

.......... .......... .......... .......... .......... ..........

4021 ttgctctagg ccgcgattaa attccaacat ggatgctgat ttatatgggt ataaatgggc 4080

4021 aacgagatcc ggcgctaatt taaggttgta cctacgacta aatataccca tatttacccg 4080

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

NruI(4083) .......... .......... ClaI(4117) .......... ..........

4081 tcgcgataat gtcgggcaat caggtgcgac aatctatcga ttgtatggga agcccgatgc 4140

4081 agcgctatta cagcccgtta gtccacgctg ttagatagct aacataccct tcgggctacg 4140

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 gccagagttg tttctgaaac atggcaaagg tagcgttgcc aatgatgtta cagatgagat 4200

4141 cggtctcaac aaagactttg taccgtttcc atcgcaacgg ttactacaat gtctactcta 4200

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ggtcagacta aactggctga cggaatttat gcctcttccg accatcaagc attttatccg 4260

4201 ccagtctgat ttgaccgact gccttaaata cggagaaggc tggtagttcg taaaataggc 4260

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

.......... .......... .......... SmaI(4300) .......... ..........

4261 tactcctgat gatgcatggt tactcaccac tgcgatcccc gggaaaacag cattccaggt 4320

4261 atgaggacta ctacgtacca atgagtggtg acgctagggg cccttttgtc gtaaggtcca 4320

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 attagaagaa tatcctgatt caggtgaaaa tattgttgat gcgctggcag tgttcctgcg 4380

4321 taatcttctt ataggactaa gtccactttt ataacaacta cgcgaccgtc acaaggacgc 4380

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ccggttgcat tcgattcctg tttgtaattg tccttttaac agcgatcgcg tatttcgtct 4440

4381 ggccaacgta agctaaggac aaacattaac aggaaaattg tcgctagcgc ataaagcaga 4440

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cgctcaggcg caatcacgaa tgaataacgg tttggttgat gcgagtgatt ttgatgacga 4500

4441 gcgagtccgc gttagtgctt acttattgcc aaaccaacta cgctcactaa aactactgct 4500

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcgtaatggc tggcctgttg aacaagtctg gaaagaaatg cataaacttt tgccattctc 4560

4501 cgcattaccg accggacaac ttgttcagac ctttctttac gtatttgaaa acggtaagag 4560

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 accggattca gtcgtcactc atggtgattt ctcacttgat aaccttattt ttgacgaggg 4620

4561 tggcctaagt cagcagtgag taccactaaa gagtgaacta ttggaataaa aactgctccc 4620

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gaaattaata ggttgtattg atgttggacg agtcggaatc gcagaccgat accaggatct 4680

4621 ctttaattat ccaacataac tacaacctgc tcagccttag cgtctggcta tggtcctaga 4680

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tgccatccta tggaactgcc tcggtgagtt ttctccttca ttacagaaac ggctttttca 4740

4681 acggtaggat accttgacgg agccactcaa aagaggaagt aatgtctttg ccgaaaaagt 4740

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aaaatatggt attgataatc ctgatatgaa taaattgcag tttcatttga tgctcgatga 4800

4741 ttttatacca taactattag gactatactt atttaacgtc aaagtaaact acgagctact 4800

.......... ...... Orf frame 2 KanR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gtttttctaa gaattaattc atgagcggat acatatttga atgtatttag aaaaataaac 4860

4801 caaaaagatt cttaattaag tactcgccta tgtataaact tacataaatc tttttatttg 4860

Orf frame 2 KanR2 .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 aaataggggt tccgcgcaca tttccccgaa aagtgccacc tgaaattgta aacgttaata 4920

4861 tttatcccca aggcgcgtgt aaaggggctt ttcacggtgg actttaacat ttgcaattat 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ttttgttaaa attcgcgtta aatttttgtt aaatcagctc attttttaac caataggccg 4980

4921 aaaacaattt taagcgcaat ttaaaaacaa tttagtcgag taaaaaattg gttatccggc 4980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaatcggcaa aatcccttat aaatcaaaag aatagaccga gatagggttg agtgttgttc 5040

4981 tttagccgtt ttagggaata tttagttttc ttatctggct ctatcccaac tcacaacaag 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cagtttggaa caagagtcca ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa 5100

5041 gtcaaacctt gttctcaggt gataatttct tgcacctgag gttgcagttt cccgcttttt 5100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ccgtctatca gggcgatggc ccactacgtg aaccatcacc ctaatcaagt tttttggggt 5160

5101 ggcagatagt cccgctaccg ggtgatgcac ttggtagtgg gattagttca aaaaacccca 5160

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 cgaggtgccg taaagcacta aatcggaacc ctaaagggag cccccgattt agagcttgac 5220

5161 gctccacggc atttcgtgat ttagccttgg gatttccctc gggggctaaa tctcgaactg 5220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa agcgaaagga gcgggcgcta 5280

5221 cccctttcgg ccgcttgcac cgctctttcc ttcccttctt tcgctttcct cgcccgcgat 5280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac cacacccgcc gcgcttaatg 5340

5281 cccgcgaccg ttcacatcgc cagtgcgacg cgcattggtg gtgtgggcgg cgcgaattac 5340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cgccgctaca gggcgcgtcc cattcgcca. .......... .......... .......... 5369

5341 gcggcgatgt cccgcgcagg gtaagcggt. .......... .......... .......... 5369

.......... .......... .......... .......... .......... ..........