Sequence of pET3a

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Sequence of pET3a

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Sequence of pET3a : 4640 BP; 1050 A; 1281 C; 1194 G; 1115 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... ......... tet(w/ gaps) .........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 240

181 cgccctatag caggtaaggc tgtcgtagcg gtcagtgata ccgcacgacg atcgcgatat 240

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 300

241 acgcaactac gttaaagata cgcgtgggca agagcctcgt gacaggctgg cgaaaccggc 300

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 360

301 ggcgggtcag gacgagcgaa gcgatgaacc tcggtgatag ctgatgcgct agtaccgctg 360

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 420

361 gtgtgggcag gacacctata ggcctatatc aaggaggaaa gtcgtttttt ggggagttct 420

.......... ......... tet(w/ gaps) T7_term ......... ..........

.......... .......... .......... .......... .......... ..........

421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 480

421 gggcaaatct ccggggttcc ccaatacgat caataacgag tcgccaccgt cgtcggttga 480

.......... .. T7_term tet(w/ gaps) T7_Terminal_pri .. ..........

.......... .......... .......... .......... .......... ..........

481 cagcttcctt tcgggctttg ttagcagccg gatccgcgac ccatttgctg tccaccagtc 540

481 gtcgaaggaa agcccgaaac aatcgtcggc ctaggcgctg ggtaaacgac aggtggtcag 540

......... T7_term tet(w/ gaps) . T7_gene10_leader T7_leader ..

NdeI(550) .......... .......... .......... .......... ..........

541 atgctagcca tatgtatatc tccttcttaa agttaaacaa aattatttct agagggaaac 600

541 tacgatcggt atacatatag aggaagaatt tca atttgtt ttaataaaga tctccctttg 600

T7_gene10_leader T7_leader Xpress_fwd_pri tet T7_transl_en_RBS

.......... .......... .......... .......... BglII(646) ..........

601 cgttgtggtc tccctatagt gagtcgtatt aatttcgcgg gatcgagatc tcgatcctct 660

601 gcaacaccag agggatatca ctcagcataa ttaaagcgcc ctagctctag agctaggaga 660

.......... ..... tet(w/ gaps) T7_pro ..... .......... ..........

.......... .......... .......... .......... .......... ..........

661 acgccggacg catcgtggcc ggcatcaccg gcgccacagg tgcggttgct ggcgcctata 720

661 tgcggcctgc gtagcaccgg ccgtagtggc cgcggtgtcc acgccaacga ccgcggatat 720

.......... ......... tet(w/ gaps) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcgccgacat caccgatggg gaagatcggg ctcgccactt cgggctcatg agcgcttgtt 780

721 agcggctgta gtggctaccc cttctagccc gagcggtgaa gcccgagtac tcgcgaacaa 780

pBRrevBam_pri ..... tet(w/ gaps) ......... .......... ORF 3

.......... .......... .......... .......... .......... ..........

781 tcggcgtggg tatggtggca ggccccgtgg ccgggggact gttgggcgcc atctccttgc 840

781 agccgcaccc ataccaccgt ccggggcacc ggccccctga caacccgcgg tagaggaacg 840

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

841 atgcaccatt ccttgcggcg gcggtgctca acggcctcaa cctactactg ggctgcttcc 900

841 tacgtggtaa ggaacgccgc cgccacgagt tgccggagtt ggatgatgac ccgacgaagg 900

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... SalI(928) .......... .......... ..........

901 taatgcagga gtcgcataag ggagagcgtc gaccgatgcc cttgagagcc ttcaacccag 960

901 attacgtcct cagcgtattc cctctcgcag ctggctacgg gaactctcgg aagttgggtc 960

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc cgcacttatg actgtcttct 1020

961 agtcgaggaa ggccacccgc gccccgtact gatagcagcg gcgtgaatac tgacagaaga 1020

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttatcatgca actcgtagga caggtgccgg cagcgctctg ggtcattttc ggcgaggacc 1080

1021 aatagtacgt tgagcatcct gtccacggcc gtcgcgagac ccagtaaaag ccgctcctgg 1080

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gctttcgctg gagcgcgacg atgatcggcc tgtcgcttgc ggtattcgga atcttgcacg 1140

1081 cgaaagcgac ctcgcgctgc tactagccgg acagcgaacg ccataagcct tagaacgtgc 1140

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ccctcgctca agccttcgtc actggtcccg ccaccaaacg tttcggcgag aagcaggcca 1200

1141 gggagcgagt tcggaagcag tgaccagggc ggtggtttgc aaagccgctc ttcgtccggt 1200

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... EagI(1216) .......... .......... .......... NruI(1251)

1201 ttatcgccgg catggcggcc gacgcgctgg gctacgtctt gctggcgttc gcgacgcgag 1260

1201 aatagcggcc gtaccgccgg ctgcgcgacc cgatgcagaa cgaccgcaag cgctgcgctc 1260

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gctggatggc cttccccatt atgattcttc tcgcttccgg cggcatcggg atgcccgcgt 1320

1261 cgacctaccg gaaggggtaa tactaagaag agcgaaggcc gccgtagccc tacgggcgca 1320

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgcaggccat gctgtccagg caggtagatg acgaccatca gggacagctt caaggatcgc 1380

1321 acgtccggta cgacaggtcc gtccatctac tgctggtagt ccctgtcgaa gttcctagcg 1380

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tcgcggctct taccagccta acttcgatca ctggaccgct gatcgtcacg gcgatttatg 1440

1381 agcgccgaga atggtcggat tgaagctagt gacctggcga ctagcagtgc cgctaaatac 1440

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ccgcctcggc gagcacatgg aacgggttgg catggattgt aggcgccgcc ctataccttg 1500

1441 ggcggagccg ctcgtgtacc ttgcccaacc gtacctaaca tccgcggcgg gatatggaac 1500

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tctgcctccc cgcgttgcgt cgcggtgcat ggagccgggc cacctcgacc tgaatggaag 1560

1501 agacggaggg gcgcaacgca gcgccacgta cctcggcccg gtggagctgg acttaccttc 1560

.......... ...... tet(w/ gaps) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ccggcggcac ctcgctaacg gattcaccac tccaagaatt ggagccaatc aattcttgcg 1620

1561 ggccgccgtg gagcgattgc ctaagtggtg aggttcttaa cctcggttag ttaagaacgc 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gagaactgtg aatgcgcaaa ccaacccttg gcagaacata tccatcgcgt ccgccatctc 1680

1621 ctcttgacac ttacgcgttt ggttgggaac cgtcttgtat aggtagcgca ggcggtagag 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... MscI(1723) ..........

1681 cagcagccgc acgcggcgca tctcgggcag cgttgggtcc tggccacggg tgcgcatgat 1740

1681 gtcgtcggcg tgcgccgcgt agagcccgtc gcaacccagg accggtgccc acgcgtacta 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgtgctcctg tcgttgagga cccggctagg ctggcggggt tgccttactg gttagcagaa 1800

1741 gcacgaggac agcaactcct gggccgatcc gaccgcccca acggaatgac caatcgtctt 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tgaatcaccg atacgcgagc gaacgtgaag cgactgctgc tgcaaaacgt ctgcgacctg 1860

1801 acttagtggc tatgcgctcg cttgcacttc gctgacgacg acgttttgca gacgctggac 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 agcaacaaca tgaatggtct tcggtttccg tgtttcgtaa agtctggaaa cgcggaagtc 1920

1861 tcgttgttgt acttaccaga agccaaaggc acaaagcatt tcagaccttt gcgccttcag 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 agcgccctgc accattatgt tccggatctg catcgcagga tgctgctggc taccctgtgg 1980

1921 tcgcgggacg tggtaataca aggcctagac gtagcgtcct acgacgaccg atgggacacc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aacacctaca tctgtattaa cgaagcgctg gcattgaccc tgagtgattt ttctctggtc 2040

1981 ttgtggatgt agacataatt gcttcgcgac cgtaactggg actcactaaa aagagaccag 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccgccgcatc cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc 2100

2041 ggcggcgtag gtatggcggt caacaaatgg gagtgttgca aggtcattgg cccgtacaag 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 atcatcagta acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat 2160

2101 tagtagtcat tgggcatagc actcgtagga gagagcaaag tagccatagt aatgggggta 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gaacagaaat cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa 2220

2161 cttgtcttta gggggaatgt gcctccgtag tcactggttt gtcctttttt ggcgggaatt 2220

.......... .......... .......... .......... ROP ..........

.......... .......... .......... .......... .......... ..........

2221 catggcccgc tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga 2280

2221 gtaccgggcg aaatagtctt cggtctgtaa ttgcgaagac ctctttgagt tgctcgacct 2280

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgcggatgaa caggcagaca tctgtgaatc gcttcacgac cacgctgatg agctttaccg 2340

2281 gcgcctactt gtccgtctgt agacacttag cgaagtgctg gtgcgactac tcgaaatggc 2340

.......... .......... ROP .......... .......... ..........

PvuII(2343) ........ .......... .......... .......... ..........

2341 cagctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga 2400

2341 gtcgacggag cgcgcaaagc cactactgcc acttttggag actgtgtacg tcgagggcct 2400

.......... .......... ROP .......... pGEX_3_pri ..........

.......... .......... .......... .......... .......... ..........

2401 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc 2460

2401 ctgccagtgt cgaacagaca ttcgcctacg gccctcgtct gttcgggcag tcccgcgcag 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt 2520

2461 tcgcccacaa ccgcccacag ccccgcgtcg gtactgggtc agtgcatcgc tatcgcctca 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gtatactggc ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatatgc 2580

2521 catatgaccg aattgatacg ccgtagtctc gtctaacatg actctcacgt ggtatatacg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ggtgtgaaat accgcacaga tgcgtaagga gaaaataccg catcaggcgc tcttccgctt 2640

2581 ccacacttta tggcgtgtct acgcattcct cttttatggc gtagtccgcg agaaggcgaa 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta tcagctcact 2700

2641 ggagcgagtg actgagcgac gcgagccagc aagccgacgc cgctcgccat agtcgagtga 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag aacatgtgag 2760

2701 gtttccgcca ttatgccaat aggtgtctta gtcccctatt gcgtcctttc ttgtacactc 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 2820

2761 gttttccggt cgttttccgg tccttggcat ttttccggcg caacgaccgc aaaaaggtat 2820

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2821 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 2880

2821 ccgaggcggg gggactgctc gtagtgtttt tagctgcgag ttcagtctcc accgctttgg 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 2940

2881 gctgtcctga tatttctatg gtccgcaaag ggggaccttc gagggagcac gcgagaggac 2940

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 3000

2941 aaggctggga cggcgaatgg cctatggaca ggcggaaaga gggaagccct tcgcaccgcg 3000

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 3060

3001 aaagagtatc gagtgcgaca tccatagagt caagccacat ccagcaagcg aggttcgacc 3060

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 3120

3061 cgacacacgt gcttgggggg caagtcgggc tggcgacgcg gaataggcca ttgatagcag 3120

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 3180

3121 aactcaggtt gggccattct gtgctgaata gcggtgaccg tcgtcggtga ccattgtcct 3180

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 3240

3181 aatcgtctcg ctccatacat ccgccacgat gtctcaagaa cttcaccacc ggattgatgc 3240

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gctacactag aaggacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 3300

3241 cgatgtgatc ttcctgtcat aaaccataga cgcgagacga cttcggtcaa tggaagcctt 3300

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 3360

3301 tttctcaacc atcgagaact aggccgtttg tttggtggcg accatcgcca ccaaaaaaac 3360

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 3420

3361 aaacgttcgt cgtctaatgc gcgtcttttt ttcctagagt tcttctagga aactagaaaa 3420

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 3480

3421 gatgccccag actgcgagtc accttgcttt tgagtgcaat tccctaaaac cagtactcta 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 3540

3481 atagtttttc ctagaagtgg atctaggaaa atttaatttt tacttcaaaa tttagttaga 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 3600

3541 tttcatatat actcatttga accagactgt caatggttac gaattagtca ctccgtggat 3600

.......... .......... .......... ....... ORF 2 Ampicillin .......

.......... .......... .......... .......... .......... ..........

3601 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 3660

3601 agagtcgcta gacagataaa gcaagtaggt atcaacggac tgaggggcag cacatctatt 3660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 3720

3661 gatgctatgc cctcccgaat ggtagaccgg ggtcacgacg ttactatggc gctctgggtg 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 3780

3721 cgagtggccg aggtctaaat agtcgttatt tggtcggtcg gccttcccgg ctcgcgtctt 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 3840

3781 caccaggacg ttgaaatagg cggaggtagg tcagataatt aacaacggcc cttcgatctc 3840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... PstI(3890) ..........

3841 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctgca ggcatcgtgg 3900

3841 attcatcaag cggtcaatta tcaaacgcgt tgcaacaacg gtaacgacgt ccgtagcacc 3900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 3960

3901 acagtgcgag cagcaaacca taccgaagta agtcgaggcc aagggttgct agttccgctc 3960

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 4020

3961 aatgtactag ggggtacaac acgttttttc gccaatcgag gaagccagga ggctagcaac 4020

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 4080

4021 agtcttcatt caaccggcgt cacaatagtg agtaccaata ccgtcgtgac gtattaagag 4080

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 4140

4081 aatgacagta cggtaggcat tctacgaaaa gacactgacc actcatgagt tggttcagta 4140

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaaca cgggataata 4200

4141 agactcttat cacatacgcc gctggctcaa cgagaacggg ccgcagttgt gccctattat 4200

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 4260

4201 ggcgcggtgt atcgtcttga aattttcacg agtagtaacc ttttgcaaga agccccgctt 4260

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 4320

4261 ttgagagttc ctagaatggc gacaactcta ggtcaagcta cattgggtga gcacgtgggt 4320

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 4380

4321 tgactagaag tcgtagaaaa tgaaagtggt cgcaaagacc cactcgtttt tgtccttccg 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 4440

4381 ttttacggcg ttttttccct tattcccgct gtgcctttac aacttatgag tatgagaagg 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 4500

4441 aaaaagttat aataacttcg taaatagtcc caataacaga gtactcgcct atgtataaac 4500

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

4501 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 4560

4501 ttacataaat ctttttattt gtttatcccc aaggcgcgtg taaaggggct tttcacggtg 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga 4620

4561 gactgcagat tctttggtaa taatagtact gtaattggat atttttatcc gcatagtgct 4620

.......... .......... .......... .......... .......... ..........

......... EcoRI(4638) ......... .......... .......... ..........

4621 ggccctttcg tcttcaagaa .......... .......... .......... .......... 4640

4621 ccgggaaagc agaagttctt .......... .......... .......... .......... 4640

.......... .......... .......... .......... .......... ..........