^_*^_*{&LOCUS pET3xa 5384 bp DNA SYN 27-Aug-2007 DEFINITION pET3xa ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5384 /organism="pET3xa" /mol_type="other DNA" terminator complement(379..507) /label="T7_terminator" misc_feature 447..465 /label="T7_Terminal_primer" CDS complement(501..1295) /label="ORF frame 3" /translation="MASMTGGQQMGTNQGKGVVAAGDKLALFLKVFGGEVLTAFARTS VTTSRHMVRSISSGKSAQFPVLGRTQAAYLAPGENLDDKRKDIKHTEKVITIDGLLTA DVLIYDIEDAMNHYDVRSEYTSQLGESLAMAADGAVLAEIAGLCNVESKYNENIEGLG TATVIETTQNKAALTDQVALGKEIIAALTKARAALTKNYVPAADRVFYCDPDSYSAIL AALMPNAANYAALIDPEKGSIRNVMGFEVVEVPHLTAGGAGTGSGC*" gene complement(86..2297) /label="tet(variant)" /gene="tet(variant)" misc_feature complement(1263..1295) /label="T7_gene10_leader" misc_feature complement(1263..1295) /label="T7_leader" misc_feature complement(1278..1296) /label="Xpress_fwd_primer" misc_feature complement(1304..1320) /label="T7_transl_en_RBS" promoter complement(1357..1375) /label="T7_promoter" misc_feature complement(1459..1478) /label="pBRrevBam_primer" CDS 1512..2297 /label="ORF frame 3" /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF LMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFG EDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLA FATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPL IVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*" misc_feature 2936..3127 /label="ROP" misc_feature complement(3121..3143) /label="pGEX_3_primer" rep_origin complement(3542..4161) /label="pBR322_origin" CDS complement(4316..5176) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4316..5176) /label="Ampicillin" /gene="Ampicillin" promoter complement(5218..5246) /label="AmpR_promoter" ORIGIN 1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA 61 TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG 121 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT 181 GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT GGCGTGCTGC TAGCGCTATA 241 TGCGTTGATG CAATTTCTAT GCGCACCCGT TCTCGGAGCA CTGTCCGACC GCTTTGGCCG 301 CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC 361 CACACCCGTC CTGTGGATAT CCGGATATAG TTCCTCCTTT CAGCAAAAAA CCCCTCAAGA 421 CCCGTTTAGA GGCCCCAAGG GGTTATGCTA GTTATTGCTC AGCGGTGGCA GCAGCCAACT 481 CAGCTTCCTT TCGGGCTTTG TTAGCAGCCG GATCCGGTAC CAGCACCACC AGCGGTGAGG 541 TGCGGAACTT CTACAACCTC AAAGCCCATA ACGTTGCGGA TAGAACCCTT CTCAGGGTCA 601 ATCAGAGCAG CGTAGTTTGC TGCGTTCGGC ATCAGTGCTG CCAGAATCGC AGAGTAGCTA 661 TCTGGGTCAC AGTAGAACAC ACGGTCAGCA GCCGGAACAT AGTTCTTGGT CAGAGCCGCA 721 CGAGCCTTAG TCAGAGCCGC AATAATCTCC TTACCCAGCG CAACTTGGTC GGTAAGTGCG 781 GCCTTGTTCT GAGTGGTCTC AATTACGGTA GCAGTACCTA AGCCCTCGAT GTTCTCATTA 841 TATTTGCTTT CCACGTTACA CAGACCGGCA ATCTCAGCCA GAACCGCACC ATCCGCAGCC 901 ATCGCCAGAG ATTCACCCAA CTGAGAGGTA TACTCAGAGC GAACGTCGTA GTGGTTCATC 961 GCGTCCTCAA TATCATAAAT CAGAACGTCA GCCGTCAGGA GACCGTCAAT GGTGATTACC 1021 TTCTCGGTGT GTTTGATGTC CTTACGTTTA TCGTCGAGGT TCTCGCCCGG AGCCAGATAC 1081 GCTGCCTGAG TGCGACCCAG AACAGGGAAC TGAGCGGATT TACCGCTGGA GATGGAACGT 1141 ACCATGTGGC GAGAAGTGGT CACGGAGGTA CGAGCGAACG CAGTCAGGAC TTCACCGCCA 1201 AATACCTTCA AGAACAACGC CAGTTTATCT CCAGCAGCAA CTACACCTTT ACCTTGGTTA 1261 GTACCCATTT GCTGTCCACC AGTCATGCTA GCCATATGTA TATCTCCTTC TTAAAGTTAA 1321 ACAAAATTAT TTCTAGAGGG AAACCGTTGT GGTCTCCCTA TAGTGAGTCG TATTAATTTC 1381 GCGGGATCGA GATCTCGATC CTCTACGCCG GACGCATCGT GGCCGGCATC ACCGGCGCCA 1441 CAGGTGCGGT TGCTGGCGCC TATATCGCCG ACATCACCGA TGGGGAAGAT CGGGCTCGCC 1501 ACTTCGGGCT CATGAGCGCT TGTTTCGGCG TGGGTATGGT GGCAGGCCCC GTGGCCGGGG 1561 GACTGTTGGG CGCCATCTCC TTGCATGCAC CATTCCTTGC GGCGGCGGTG CTCAACGGCC 1621 TCAACCTACT ACTGGGCTGC TTCCTAATGC AGGAGTCGCA TAAGGGAGAG CGTCGACCGA 1681 TGCCCTTGAG AGCCTTCAAC CCAGTCAGCT CCTTCCGGTG GGCGCGGGGC ATGACTATCG 1741 TCGCCGCACT TATGACTGTC TTCTTTATCA TGCAACTCGT AGGACAGGTG CCGGCAGCGC 1801 TCTGGGTCAT TTTCGGCGAG GACCGCTTTC GCTGGAGCGC GACGATGATC GGCCTGTCGC 1861 TTGCGGTATT CGGAATCTTG CACGCCCTCG CTCAAGCCTT CGTCACTGGT CCCGCCACCA 1921 AACGTTTCGG CGAGAAGCAG GCCATTATCG CCGGCATGGC GGCCGACGCG CTGGGCTACG 1981 TCTTGCTGGC GTTCGCGACG CGAGGCTGGA TGGCCTTCCC CATTATGATT CTTCTCGCTT 2041 CCGGCGGCAT CGGGATGCCC GCGTTGCAGG CCATGCTGTC CAGGCAGGTA GATGACGACC 2101 ATCAGGGACA GCTTCAAGGA TCGCTCGCGG CTCTTACCAG CCTAACTTCG ATCACTGGAC 2161 CGCTGATCGT CACGGCGATT TATGCCGCCT CGGCGAGCAC ATGGAACGGG TTGGCATGGA 2221 TTGTAGGCGC CGCCCTATAC CTTGTCTGCC TCCCCGCGTT GCGTCGCGGT GCATGGAGCC 2281 GGGCCACCTC GACCTGAATG GAAGCCGGCG GCACCTCGCT AACGGATTCA CCACTCCAAG 2341 AATTGGAGCC AATCAATTCT TGCGGAGAAC TGTGAATGCG CAAACCAACC CTTGGCAGAA 2401 CATATCCATC GCGTCCGCCA TCTCCAGCAG CCGCACGCGG CGCATCTCGG GCAGCGTTGG 2461 GTCCTGGCCA CGGGTGCGCA TGATCGTGCT CCTGTCGTTG AGGACCCGGC TAGGCTGGCG 2521 GGGTTGCCTT ACTGGTTAGC AGAATGAATC ACCGATACGC GAGCGAACGT GAAGCGACTG 2581 CTGCTGCAAA ACGTCTGCGA CCTGAGCAAC AACATGAATG GTCTTCGGTT TCCGTGTTTC 2641 GTAAAGTCTG GAAACGCGGA AGTCAGCGCC CTGCACCATT ATGTTCCGGA TCTGCATCGC 2701 AGGATGCTGC TGGCTACCCT GTGGAACACC TACATCTGTA TTAACGAAGC GCTGGCATTG 2761 ACCCTGAGTG ATTTTTCTCT GGTCCCGCCG CATCCATACC GCCAGTTGTT TACCCTCACA 2821 ACGTTCCAGT AACCGGGCAT GTTCATCATC AGTAACCCGT ATCGTGAGCA TCCTCTCTCG 2881 TTTCATCGGT ATCATTACCC CCATGAACAG AAATCCCCCT TACACGGAGG CATCAGTGAC 2941 CAAACAGGAA AAAACCGCCC TTAACATGGC CCGCTTTATC AGAAGCCAGA CATTAACGCT 3001 TCTGGAGAAA CTCAACGAGC TGGACGCGGA TGAACAGGCA GACATCTGTG AATCGCTTCA 3061 CGACCACGCT GATGAGCTTT ACCGCAGCTG CCTCGCGCGT TTCGGTGATG ACGGTGAAAA 3121 CCTCTGACAC ATGCAGCTCC CGGAGACGGT CACAGCTTGT CTGTAAGCGG ATGCCGGGAG 3181 CAGACAAGCC CGTCAGGGCG CGTCAGCGGG TGTTGGCGGG TGTCGGGGCG CAGCCATGAC 3241 CCAGTCACGT AGCGATAGCG GAGTGTATAC TGGCTTAACT ATGCGGCATC AGAGCAGATT 3301 GTACTGAGAG TGCACCATAT ATGCGGTGTG AAATACCGCA CAGATGCGTA AGGAGAAAAT 3361 ACCGCATCAG GCGCTCTTCC GCTTCCTCGC TCACTGACTC GCTGCGCTCG GTCGTTCGGC 3421 TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG 3481 ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG 3541 CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC 3601 GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG 3661 GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT 3721 TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG 3781 TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT 3841 GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC 3901 TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT 3961 TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGGAC AGTATTTGGT ATCTGCGCTC 4021 TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA 4081 CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT 4141 CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC 4201 GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT 4261 AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA AACTTGGTCT GACAGTTACC 4321 AATGCTTAAT CAGTGAGGCA CCTATCTCAG CGATCTGTCT ATTTCGTTCA TCCATAGTTG 4381 CCTGACTCCC CGTCGTGTAG ATAACTACGA TACGGGAGGG CTTACCATCT GGCCCCAGTG 4441 CTGCAATGAT ACCGCGAGAC CCACGCTCAC CGGCTCCAGA TTTATCAGCA ATAAACCAGC 4501 CAGCCGGAAG GGCCGAGCGC AGAAGTGGTC CTGCAACTTT ATCCGCCTCC ATCCAGTCTA 4561 TTAATTGTTG CCGGGAAGCT AGAGTAAGTA GTTCGCCAGT TAATAGTTTG CGCAACGTTG 4621 TTGCCATTGC TGCAGGCATC GTGGTGTCAC GCTCGTCGTT TGGTATGGCT TCATTCAGCT 4681 CCGGTTCCCA ACGATCAAGG CGAGTTACAT GATCCCCCAT GTTGTGCAAA AAAGCGGTTA 4741 GCTCCTTCGG TCCTCCGATC GTTGTCAGAA GTAAGTTGGC CGCAGTGTTA TCACTCATGG 4801 TTATGGCAGC ACTGCATAAT TCTCTTACTG TCATGCCATC CGTAAGATGC TTTTCTGTGA 4861 CTGGTGAGTA CTCAACCAAG TCATTCTGAG AATAGTGTAT GCGGCGACCG AGTTGCTCTT 4921 GCCCGGCGTC AACACGGGAT AATACCGCGC CACATAGCAG AACTTTAAAA GTGCTCATCA 4981 TTGGAAAACG TTCTTCGGGG CGAAAACTCT CAAGGATCTT ACCGCTGTTG AGATCCAGTT 5041 CGATGTAACC CACTCGTGCA CCCAACTGAT CTTCAGCATC TTTTACTTTC ACCAGCGTTT 5101 CTGGGTGAGC AAAAACAGGA AGGCAAAATG CCGCAAAAAA GGGAATAAGG GCGACACGGA 5161 AATGTTGAAT ACTCATACTC TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT 5221 GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC 5281 GCACATTTCC CCGAAAAGTG CCACCTGACG TCTAAGAAAC CATTATTATC ATGACATTAA 5341 CCTATAAAAA TAGGCGTATC ACGAGGCCCT TTCGTCTTCA AGAA //pET3xa "Times New Roman^_*C{T7_term"Times New Roman^_*V tet(variant)"Times New Roman^_*CT7_Terminal_pri"Times New Roman)^_*CɆORF 3"Times New Roman)^_*CT7_gene10_leader"Times New Roman^_*C T7_leader"Times New Roman>^_*CɆXpress_fwd_pri"Times New RomanS^_*(T7_transl_en_RBS"Times New Roman^_*CM_T7_pro"Times New Roman^_*C pBRrevBam_pri"Times New Roman^_*CɆORF 3"Times New Roman^_*x 7 ROP"Times New Roman^_*C1 G  pGEX_3_pri"Times New Roman^_*C A pBR322_origin"Times New Roman^_*CɆ8ORF 2"Times New Roman^_*8 Ampicillin"Times New Roman^_*Cb~AmpR_pro"Times New Roman ^_*ClaI(24),HindIII(29)"Times New Roman}^}J88 ^_*BamHI(510),KpnI(520)"Times New RomantcEVc ^_*!NdeI(1294),XbaI(1332),BglII(1390)"Times New Roman^3r20F ^_*SalI"Times New RomanObtt ^_*EagI(1960),NruI(1995)"Times New Roman&5DD ^_*MscI"Times New Roman !4GGV ^_*PvuII"Times New Roman  $55#D ^_*PstI"Times New Romank ^_*AatII(5311),EcoRI(5382)"Times New Romand^pJa6a6 ^_*test"Times New Romanx5  ^_*test"Times New Roman^TCQ1L 1