Sequence of pET3xa

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Sequence of pET3xa

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Sequence of pET3xa : 5384 BP; 1237 A; 1486 C; 1379 G; 1282 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... ......... tet(variant) .........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 240

181 cgccctatag caggtaaggc tgtcgtagcg gtcagtgata ccgcacgacg atcgcgatat 240

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 300

241 acgcaactac gttaaagata cgcgtgggca agagcctcgt gacaggctgg cgaaaccggc 300

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 360

301 ggcgggtcag gacgagcgaa gcgatgaacc tcggtgatag ctgatgcgct agtaccgctg 360

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 420

361 gtgtgggcag gacacctata ggcctatatc aaggaggaaa gtcgtttttt ggggagttct 420

.......... ......... tet(variant) T7_term ......... ..........

.......... .......... .......... .......... .......... ..........

421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 480

421 gggcaaatct ccggggttcc ccaatacgat caataacgag tcgccaccgt cgtcggttga 480

.......... .. T7_term tet(variant) T7_Terminal_pri .. ..........

.......... .......... BamHI(510) KpnI(520) .......... ..........

481 cagcttcctt tcgggctttg ttagcagccg gatccggtac cagcaccacc agcggtgagg 540

481 gtcgaaggaa agcccgaaac aatcgtcggc ctaggccatg gtcgtggtgg tcgccactcc 540

......... T7_term tet(variant) ORF 3 ......... ..........

.......... .......... .......... .......... .......... ..........

541 tgcggaactt ctacaacctc aaagcccata acgttgcgga tagaaccctt ctcagggtca 600

541 acgccttgaa gatgttggag tttcgggtat tgcaacgcct atcttgggaa gagtcccagt 600

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 atcagagcag cgtagtttgc tgcgttcggc atcagtgctg ccagaatcgc agagtagcta 660

601 tagtctcgtc gcatcaaacg acgcaagccg tagtcacgac ggtcttagcg tctcatcgat 660

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tctgggtcac agtagaacac acggtcagca gccggaacat agttcttggt cagagccgca 720

661 agacccagtg tcatcttgtg tgccagtcgt cggccttgta tcaagaacca gtctcggcgt 720

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cgagccttag tcagagccgc aataatctcc ttacccagcg caacttggtc ggtaagtgcg 780

721 gctcggaatc agtctcggcg ttattagagg aatgggtcgc gttgaaccag ccattcacgc 780

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gccttgttct gagtggtctc aattacggta gcagtaccta agccctcgat gttctcatta 840

781 cggaacaaga ctcaccagag ttaatgccat cgtcatggat tcgggagcta caagagtaat 840

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tatttgcttt ccacgttaca cagaccggca atctcagcca gaaccgcacc atccgcagcc 900

841 ataaacgaaa ggtgcaatgt gtctggccgt tagagtcggt cttggcgtgg taggcgtcgg 900

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

901 atcgccagag attcacccaa ctgagaggta tactcagagc gaacgtcgta gtggttcatc 960

901 tagcggtctc taagtgggtt gactctccat atgagtctcg cttgcagcat caccaagtag 960

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 gcgtcctcaa tatcataaat cagaacgtca gccgtcagga gaccgtcaat ggtgattacc 1020

961 cgcaggagtt atagtattta gtcttgcagt cggcagtcct ctggcagtta ccactaatgg 1020

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttctcggtgt gtttgatgtc cttacgttta tcgtcgaggt tctcgcccgg agccagatac 1080

1021 aagagccaca caaactacag gaatgcaaat agcagctcca agagcgggcc tcggtctatg 1080

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 gctgcctgag tgcgacccag aacagggaac tgagcggatt taccgctgga gatggaacgt 1140

1081 cgacggactc acgctgggtc ttgtcccttg actcgcctaa atggcgacct ctaccttgca 1140

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 accatgtggc gagaagtggt cacggaggta cgagcgaacg cagtcaggac ttcaccgcca 1200

1141 tggtacaccg ctcttcacca gtgcctccat gctcgcttgc gtcagtcctg aagtggcggt 1200

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aataccttca agaacaacgc cagtttatct ccagcagcaa ctacaccttt accttggtta 1260

1201 ttatggaagt tcttgttgcg gtcaaataga ggtcgtcgtt gatgtggaaa tggaaccaat 1260

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gtacccattt gctgtccacc agtcatgcta gccatatgta tatctccttc ttaaagttaa 1320

1261 catgggtaaa cgacaggtgg tcagtacgat cggtatacat atagaggaag aatttcaatt 1320

ORF 3 T7_gene10_leader T7_leader tet Xpress_fwd_pri T7_transl_en_RBS

.......... .......... .......... .......... .......... ..........

1321 acaaaattat ttctagaggg aaaccgttgt ggtctcccta tagtgagtcg tattaatttc 1380

1321 tgttttaata aagatctccc tttggcaaca ccagagggat atcactcagc a taattaaag 1380

.......... ......... tet(variant) ......... T7_pro ..........

BglII(1390) ........ .......... .......... .......... ..........

1381 gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca 1440

1381 cgccctagct ctagagctag gagatgcggc ctgcgtagca ccggccgtag tggccgcggt 1440

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc 1500

1441 gtccacgcca acgaccgcgg atatagcggc tgtagtggct accccttcta gcccgagcgg 1500

.......... .. tet(variant) pBRrevBam_pri .. .......... ..........

.......... .......... .......... .......... .......... ..........

1501 acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg 1560

1501 tgaagcccga gtactcgcga acaaagccgc acccatacca ccgtccgggg caccggcccc 1560

.......... ......... tet(variant) ORF 3 ......... ..........

.......... .......... .......... .......... .......... ..........

1561 gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc 1620

1561 ctgacaaccc gcggtagagg aacgtacgtg gtaaggaacg ccgccgccac gagttgccgg 1620

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... SalI(1672)

1621 tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga 1680

1621 agttggatga tgacccgacg aaggattacg tcctcagcgt attccctctc gcagctggct 1680

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg 1740

1681 acgggaactc tcggaagttg ggtcagtcga ggaaggccac ccgcgccccg tactgatagc 1740

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc 1800

1741 agcggcgtga atactgacag aagaaatagt acgttgagca tcctgtccac ggccgtcgcg 1800

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc 1860

1801 agacccagta aaagccgctc ctggcgaaag cgacctcgcg ctgctactag ccggacagcg 1860

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca 1920

1861 aacgccataa gccttagaac gtgcgggagc gagttcggaa gcagtgacca gggcggtggt 1920

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg 1980

1921 ttgcaaagcc gctcttcgtc cggtaatagc ggccgtaccg ccggctgcgc gacccgatgc 1980

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... NruI(1995) .......... .......... .......... ..........

1981 tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt 2040

1981 agaacgaccg caagcgctgc gctccgacct accggaaggg gtaatactaa gaagagcgaa 2040

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc 2100

2041 ggccgccgta gccctacggg cgcaacgtcc ggtacgacag gtccgtccat ctactgctgg 2100

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac 2160

2101 tagtccctgt cgaagttcct agcgagcgcc gagaatggtc ggattgaagc tagtgacctg 2160

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga 2220

2161 gcgactagca gtgccgctaa atacggcgga gccgctcgtg taccttgccc aaccgtacct 2220

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc 2280

2221 aacatccgcg gcgggatatg gaacagacgg aggggcgcaa cgcagcgcca cgtacctcgg 2280

.......... ...... tet(variant) ORF 3 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag 2340

2281 cccggtggag ctggacttac cttcggccgc cgtggagcga ttgcctaagt ggtgaggttc 2340

tet(variant) ORF 3 .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa 2400

2341 ttaacctcgg ttagttaaga acgcctcttg acacttacgc gtttggttgg gaaccgtctt 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg 2460

2401 gtataggtag cgcaggcggt agaggtcgtc ggcgtgcgcc gcgtagagcc cgtcgcaacc 2460

.......... .......... .......... .......... .......... ..........

MscI(2467) .......... .......... .......... .......... ..........

2461 gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg 2520

2461 caggaccggt gcccacgcgt actagcacga ggacagcaac tcctgggccg atccgaccgc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg 2580

2521 cccaacggaa tgaccaatcg tcttacttag tggctatgcg ctcgcttgca cttcgctgac 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc 2640

2581 gacgacgttt tgcagacgct ggactcgttg ttgtacttac cagaagccaa aggcacaaag 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga tctgcatcgc 2700

2641 catttcagac ctttgcgcct tcagtcgcgg gacgtggtaa tacaaggcct agacgtagcg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc gctggcattg 2760

2701 tcctacgacg accgatggga caccttgtgg atgtagacat aattgcttcg cgaccgtaac 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt taccctcaca 2820

2761 tgggactcac taaaaagaga ccagggcggc gtaggtatgg cggtcaacaa atgggagtgt 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca tcctctctcg 2880

2821 tgcaaggtca ttggcccgta caagtagtag tcattgggca tagcactcgt aggagagagc 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg catcagtgac 2940

2881 aaagtagcca tagtaatggg ggtacttgtc tttaggggga atgtgcctcc gtagtcactg 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct 3000

2941 gtttgtcctt ttttggcggg aattgtaccg ggcgaaatag tcttcggtct gtaattgcga 3000

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca 3060

3001 agacctcttt gagttgctcg acctgcgcct acttgtccgt ctgtagacac ttagcgaagt 3060

.......... .......... ROP .......... .......... ..........

.......... ......... PvuII(3087) ......... .......... ..........

3061 cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa 3120

3061 gctggtgcga ctactcgaaa tggcgtcgac ggagcgcgca aagccactac tgccactttt 3120

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag 3180

3121 ggagactgtg tacgtcgagg gcctctgcca gtgtcgaaca gacattcgcc tacggccctc 3180

.......... pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac 3240

3181 gtctgttcgg gcagtcccgc gcagtcgccc acaaccgccc acagccccgc gtcggtactg 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt 3300

3241 ggtcagtgca tcgctatcgc ctcacatatg accgaattga tacgccgtag tctcgtctaa 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta aggagaaaat 3360

3301 catgactctc acgtggtata tacgccacac tttatggcgt gtctacgcat tcctctttta 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 3420

3361 tggcgtagtc cgcgagaagg cgaaggagcg agtgactgag cgacgcgagc cagcaagccg 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 3480

3421 acgccgctcg ccatagtcga gtgagtttcc gccattatgc caataggtgt cttagtcccc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 3540

3481 tattgcgtcc tttcttgtac actcgttttc cggtcgtttt ccggtccttg gcatttttcc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 3600

3541 ggcgcaacga ccgcaaaaag gtatccgagg cggggggact gctcgtagtg tttttagctg 3600

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3601 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 3660

3601 cgagttcagt ctccaccgct ttgggctgtc ctgatatttc tatggtccgc aaagggggac 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 3720

3661 cttcgaggga gcacgcgaga ggacaaggct gggacggcga atggcctatg gacaggcgga 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg 3780

3721 aagagggaag cccttcgcac cgcgaaagag tatcgagtgc gacatccata gagtcaagcc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 3840

3781 acatccagca agcgaggttc gacccgacac acgtgcttgg ggggcaagtc gggctggcga 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 3900

3841 cgcggaatag gccattgata gcagaactca ggttgggcca ttctgtgctg aatagcggtg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 3960

3901 accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat acatccgcca cgatgtctca 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 4020

3961 agaacttcac caccggattg atgccgatgt gatcttcctg tcataaacca tagacgcgag 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 4080

4021 acgacttcgg tcaatggaag cctttttctc aaccatcgag aactaggccg tttgtttggt 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 4140

4081 ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct ttttttccta 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 4200

4141 gagttcttct aggaaactag aaaagatgcc ccagactgcg agtcaccttg cttttgagtg 4200

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 4260

4201 caattcccta aaaccagtac tctaatagtt tttcctagaa gtggatctag gaaaatttaa 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 4320

4261 tttttacttc aaaatttagt tagatttcat atatactcat ttgaaccaga ctgtcaatgg 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 4380

4321 ttacgaatta gtcactccgt ggatagagtc gctagacaga taaagcaagt aggtatcaac 4380

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 4440

4381 ggactgaggg gcagcacatc tattgatgct atgccctccc gaatggtaga ccggggtcac 4440

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 4500

4441 gacgttacta tggcgctctg ggtgcgagtg gccgaggtct aaatagtcgt tatttggtcg 4500

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 4560

4501 gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa taggcggagg taggtcagat 4560

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 4620

4561 aattaacaac ggcccttcga tctcattcat caagcggtca attatcaaac gcgttgcaac 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... PstI(4634) .......... .......... .......... ..........

4621 ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 4680

4621 aacggtaacg acgtccgtag caccacagtg cgagcagcaa accataccga agtaagtcga 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 4740

4681 ggccaagggt tgctagttcc gctcaatgta ctagggggta caacacgttt tttcgccaat 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 4800

4741 cgaggaagcc aggaggctag caacagtctt cattcaaccg gcgtcacaat agtgagtacc 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 4860

4801 aataccgtcg tgacgtatta agagaatgac agtacggtag gcattctacg aaaagacact 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 4920

4861 gaccactcat gagttggttc agtaagactc ttatcacata cgccgctggc tcaacgagaa 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 4980

4921 cgggccgcag ttgtgcccta ttatggcgcg gtgtatcgtc ttgaaatttt cacgagtagt 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 5040

4981 aaccttttgc aagaagcccc gcttttgaga gttcctagaa tggcgacaac tctaggtcaa 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 5100

5041 gctacattgg gtgagcacgt gggttgacta gaagtcgtag aaaatgaaag tggtcgcaaa 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 5160

5101 gacccactcg tttttgtcct tccgttttac ggcgtttttt cccttattcc cgctgtgcct 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 5220

5161 ttacaactta tgagtatgag aaggaaaaag ttataataac ttcgtaaata gtcccaataa 5220

ORF 2 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 5280

5221 cagagtactc gcctatgtat aaacttacat aaatcttttt atttgtttat ccccaaggcg 5280

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa 5340

5281 cgtgtaaagg ggcttttcac ggtggactgc agattctttg gtaataatag tactgtaatt 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... EcoRI(5382) .........

5341 cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa...... .......... 5384

5341 ggatattttt atccgcatag tgctccggga aagcagaagt tctt...... .......... 5384

.......... .......... .......... .......... .......... ..........