^K_K&LOCUS pET3xb 5386 bp DNA SYN 27-Sep-2007 DEFINITION pET3xb ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5386 /organism="pET3xb" /mol_type="other DNA" terminator complement(379..507) /label="T7_terminator" misc_feature 447..465 /label="T7_Terminal_primer" CDS complement(452..1297) /label="ORF frame 2" /translation="MASMTGGQQMGTNQGKGVVAAGDKLALFLKVFGGEVLTAFARTS VTTSRHMVRSISSGKSAQFPVLGRTQAAYLAPGENLDDKRKDIKHTEKVITIDGLLTA DVLIYDIEDAMNHYDVRSEYTSQLGESLAMAADGAVLAEIAGLCNVESKYNENIEGLG TATVIETTQNKAALTDQVALGKEIIAALTKARAALTKNYVPAADRVFYCDPDSYSAIL AALMPNAANYAALIDPEKGSIRNVMGFEVVEVPHLTAGGAGTADPAANKARKEAELAA ATAEQ*" gene complement(86..2299) /label="tet(variant)" /gene="tet(variant)" misc_feature complement(1265..1297) /label="T7_gene10_leader" misc_feature complement(1265..1297) /label="T7_leader" misc_feature complement(1280..1298) /label="Xpress_fwd_primer" misc_feature complement(1306..1322) /label="T7_transl_en_RBS" promoter complement(1359..1377) /label="T7_promoter" misc_feature complement(1461..1480) /label="pBRrevBam_primer" CDS 1514..2299 /label="ORF frame 2" /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF LMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFG EDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLA FATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPL IVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*" misc_feature 2938..3129 /label="ROP" misc_feature complement(3123..3145) /label="pGEX_3_primer" rep_origin complement(3544..4163) /label="pBR322_origin" CDS complement(4318..5178) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4318..5178) /label="Ampicillin" /gene="Ampicillin" promoter complement(5220..5248) /label="AmpR_promoter" ORIGIN 1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA 61 TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG 121 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT 181 GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT GGCGTGCTGC TAGCGCTATA 241 TGCGTTGATG CAATTTCTAT GCGCACCCGT TCTCGGAGCA CTGTCCGACC GCTTTGGCCG 301 CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC 361 CACACCCGTC CTGTGGATAT CCGGATATAG TTCCTCCTTT CAGCAAAAAA CCCCTCAAGA 421 CCCGTTTAGA GGCCCCAAGG GGTTATGCTA GTTATTGCTC AGCGGTGGCA GCAGCCAACT 481 CAGCTTCCTT TCGGGCTTTG TTAGCAGCCG GATCCGCGGT ACCAGCACCA CCAGCGGTGA 541 GGTGCGGAAC TTCTACAACC TCAAAGCCCA TAACGTTGCG GATAGAACCC TTCTCAGGGT 601 CAATCAGAGC AGCGTAGTTT GCTGCGTTCG GCATCAGTGC TGCCAGAATC GCAGAGTAGC 661 TATCTGGGTC ACAGTAGAAC ACACGGTCAG CAGCCGGAAC ATAGTTCTTG GTCAGAGCCG 721 CACGAGCCTT AGTCAGAGCC GCAATAATCT CCTTACCCAG CGCAACTTGG TCGGTAAGTG 781 CGGCCTTGTT CTGAGTGGTC TCAATTACGG TAGCAGTACC TAAGCCCTCG ATGTTCTCAT 841 TATATTTGCT TTCCACGTTA CACAGACCGG CAATCTCAGC CAGAACCGCA CCATCCGCAG 901 CCATCGCCAG AGATTCACCC AACTGAGAGG TATACTCAGA GCGAACGTCG TAGTGGTTCA 961 TCGCGTCCTC AATATCATAA ATCAGAACGT CAGCCGTCAG GAGACCGTCA ATGGTGATTA 1021 CCTTCTCGGT GTGTTTGATG TCCTTACGTT TATCGTCGAG GTTCTCGCCC GGAGCCAGAT 1081 ACGCTGCCTG AGTGCGACCC AGAACAGGGA ACTGAGCGGA TTTACCGCTG GAGATGGAAC 1141 GTACCATGTG GCGAGAAGTG GTCACGGAGG TACGAGCGAA CGCAGTCAGG ACTTCACCGC 1201 CAAATACCTT CAAGAACAAC GCCAGTTTAT CTCCAGCAGC AACTACACCT TTACCTTGGT 1261 TAGTACCCAT TTGCTGTCCA CCAGTCATGC TAGCCATATG TATATCTCCT TCTTAAAGTT 1321 AAACAAAATT ATTTCTAGAG GGAAACCGTT GTGGTCTCCC TATAGTGAGT CGTATTAATT 1381 TCGCGGGATC GAGATCTCGA TCCTCTACGC CGGACGCATC GTGGCCGGCA TCACCGGCGC 1441 CACAGGTGCG GTTGCTGGCG CCTATATCGC CGACATCACC GATGGGGAAG ATCGGGCTCG 1501 CCACTTCGGG CTCATGAGCG CTTGTTTCGG CGTGGGTATG GTGGCAGGCC CCGTGGCCGG 1561 GGGACTGTTG GGCGCCATCT CCTTGCATGC ACCATTCCTT GCGGCGGCGG TGCTCAACGG 1621 CCTCAACCTA CTACTGGGCT GCTTCCTAAT GCAGGAGTCG CATAAGGGAG AGCGTCGACC 1681 GATGCCCTTG AGAGCCTTCA ACCCAGTCAG CTCCTTCCGG TGGGCGCGGG GCATGACTAT 1741 CGTCGCCGCA CTTATGACTG TCTTCTTTAT CATGCAACTC GTAGGACAGG TGCCGGCAGC 1801 GCTCTGGGTC ATTTTCGGCG AGGACCGCTT TCGCTGGAGC GCGACGATGA TCGGCCTGTC 1861 GCTTGCGGTA TTCGGAATCT TGCACGCCCT CGCTCAAGCC TTCGTCACTG GTCCCGCCAC 1921 CAAACGTTTC GGCGAGAAGC AGGCCATTAT CGCCGGCATG GCGGCCGACG CGCTGGGCTA 1981 CGTCTTGCTG GCGTTCGCGA CGCGAGGCTG GATGGCCTTC CCCATTATGA TTCTTCTCGC 2041 TTCCGGCGGC ATCGGGATGC CCGCGTTGCA GGCCATGCTG TCCAGGCAGG TAGATGACGA 2101 CCATCAGGGA CAGCTTCAAG GATCGCTCGC GGCTCTTACC AGCCTAACTT CGATCACTGG 2161 ACCGCTGATC GTCACGGCGA TTTATGCCGC CTCGGCGAGC ACATGGAACG GGTTGGCATG 2221 GATTGTAGGC GCCGCCCTAT ACCTTGTCTG CCTCCCCGCG TTGCGTCGCG GTGCATGGAG 2281 CCGGGCCACC TCGACCTGAA TGGAAGCCGG CGGCACCTCG CTAACGGATT CACCACTCCA 2341 AGAATTGGAG CCAATCAATT CTTGCGGAGA ACTGTGAATG CGCAAACCAA CCCTTGGCAG 2401 AACATATCCA TCGCGTCCGC CATCTCCAGC AGCCGCACGC GGCGCATCTC GGGCAGCGTT 2461 GGGTCCTGGC CACGGGTGCG CATGATCGTG CTCCTGTCGT TGAGGACCCG GCTAGGCTGG 2521 CGGGGTTGCC TTACTGGTTA GCAGAATGAA TCACCGATAC GCGAGCGAAC GTGAAGCGAC 2581 TGCTGCTGCA AAACGTCTGC GACCTGAGCA ACAACATGAA TGGTCTTCGG TTTCCGTGTT 2641 TCGTAAAGTC TGGAAACGCG GAAGTCAGCG CCCTGCACCA TTATGTTCCG GATCTGCATC 2701 GCAGGATGCT GCTGGCTACC CTGTGGAACA CCTACATCTG TATTAACGAA GCGCTGGCAT 2761 TGACCCTGAG TGATTTTTCT CTGGTCCCGC CGCATCCATA CCGCCAGTTG TTTACCCTCA 2821 CAACGTTCCA GTAACCGGGC ATGTTCATCA TCAGTAACCC GTATCGTGAG CATCCTCTCT 2881 CGTTTCATCG GTATCATTAC CCCCATGAAC AGAAATCCCC CTTACACGGA GGCATCAGTG 2941 ACCAAACAGG AAAAAACCGC CCTTAACATG GCCCGCTTTA TCAGAAGCCA GACATTAACG 3001 CTTCTGGAGA AACTCAACGA GCTGGACGCG GATGAACAGG CAGACATCTG TGAATCGCTT 3061 CACGACCACG CTGATGAGCT TTACCGCAGC TGCCTCGCGC GTTTCGGTGA TGACGGTGAA 3121 AACCTCTGAC ACATGCAGCT CCCGGAGACG GTCACAGCTT GTCTGTAAGC GGATGCCGGG 3181 AGCAGACAAG CCCGTCAGGG CGCGTCAGCG GGTGTTGGCG GGTGTCGGGG CGCAGCCATG 3241 ACCCAGTCAC GTAGCGATAG CGGAGTGTAT ACTGGCTTAA CTATGCGGCA TCAGAGCAGA 3301 TTGTACTGAG AGTGCACCAT ATATGCGGTG TGAAATACCG CACAGATGCG TAAGGAGAAA 3361 ATACCGCATC AGGCGCTCTT CCGCTTCCTC GCTCACTGAC TCGCTGCGCT CGGTCGTTCG 3421 GCTGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 3481 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 3541 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 3601 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 3661 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 3721 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 3781 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 3841 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 3901 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 3961 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGG ACAGTATTTG GTATCTGCGC 4021 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 4081 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 4141 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 4201 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 4261 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAAACTTGGT CTGACAGTTA 4321 CCAATGCTTA ATCAGTGAGG CACCTATCTC AGCGATCTGT CTATTTCGTT CATCCATAGT 4381 TGCCTGACTC CCCGTCGTGT AGATAACTAC GATACGGGAG GGCTTACCAT CTGGCCCCAG 4441 TGCTGCAATG ATACCGCGAG ACCCACGCTC ACCGGCTCCA GATTTATCAG CAATAAACCA 4501 GCCAGCCGGA AGGGCCGAGC GCAGAAGTGG TCCTGCAACT TTATCCGCCT CCATCCAGTC 4561 TATTAATTGT TGCCGGGAAG CTAGAGTAAG TAGTTCGCCA GTTAATAGTT TGCGCAACGT 4621 TGTTGCCATT GCTGCAGGCA TCGTGGTGTC ACGCTCGTCG TTTGGTATGG CTTCATTCAG 4681 CTCCGGTTCC CAACGATCAA GGCGAGTTAC ATGATCCCCC ATGTTGTGCA AAAAAGCGGT 4741 TAGCTCCTTC GGTCCTCCGA TCGTTGTCAG AAGTAAGTTG GCCGCAGTGT TATCACTCAT 4801 GGTTATGGCA GCACTGCATA ATTCTCTTAC TGTCATGCCA TCCGTAAGAT GCTTTTCTGT 4861 GACTGGTGAG TACTCAACCA AGTCATTCTG AGAATAGTGT ATGCGGCGAC CGAGTTGCTC 4921 TTGCCCGGCG TCAACACGGG ATAATACCGC GCCACATAGC AGAACTTTAA AAGTGCTCAT 4981 CATTGGAAAA CGTTCTTCGG GGCGAAAACT CTCAAGGATC TTACCGCTGT TGAGATCCAG 5041 TTCGATGTAA CCCACTCGTG CACCCAACTG ATCTTCAGCA TCTTTTACTT TCACCAGCGT 5101 TTCTGGGTGA GCAAAAACAG GAAGGCAAAA TGCCGCAAAA AAGGGAATAA GGGCGACACG 5161 GAAATGTTGA ATACTCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA 5221 TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC 5281 GCGCACATTT CCCCGAAAAG TGCCACCTGA CGTCTAAGAA ACCATTATTA TCATGACATT 5341 AACCTATAAA AATAGGCGTA TCACGAGGCC CTTTCGTCTT CAAGAA // pET3xb "Times New Roman_KC {T7_term"Times New Roman_K V tet(variant)"Times New Roman_KC T7_Terminal_pri"Times New Roman)_KCɆ ORF 2"Times New Roman<_KC T7_gene10_leader"Times New Roman_KC  T7_leader"Times New Roman)_KCɆ Xpress_fwd_pri"Times New RomanQ_K *T7_transl_en_RBS"Times New Roman_KC OaT7_pro"Times New Roman_KC  pBRrevBam_pri"Times New Roman_KCɆ ORF 2"Times New Roman_K z 9 ROP"Times New Roman_KC 3 I  pGEX_3_pri"Times New Roman_KC  C pBR322_origin"Times New Roman_KCɆ :ORF 1"Times New Roman_K : Ampicillin"Times New Roman_KC dAmpR_pro"Times New Roman _KClaI(24),HindIII(29)"Times New Roman }}kZ<Z _KBamHI(510),SacII(517),KpnI(522)"Times New Roman WV _K!NdeI(1296),XbaI(1334),BglII(1392)"Times New Roman ^TrSQ;g _KSalI"Times New Roman Obtt _KEagI(1962),NruI(1997)"Times New Roman &5 DD4 _KMscI"Times New Roman  CViix _KPvuII"Times New Roman  3 EVV#e _KPstI"Times New Roman k _KAatII(5313),EcoRI(5384)"Times New Roman dukaW9aW _Ktest"Times New Roman =  _Ktest"Times New Romano ^mEn4q.4