Sequence of pET3xb

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Sequence of pET3xb

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Sequence of pET3xb : 5386 BP; 1237 A; 1487 C; 1380 G; 1282 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... ......... tet(variant) .........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 240

181 cgccctatag caggtaaggc tgtcgtagcg gtcagtgata ccgcacgacg atcgcgatat 240

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 300

241 acgcaactac gttaaagata cgcgtgggca agagcctcgt gacaggctgg cgaaaccggc 300

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 360

301 ggcgggtcag gacgagcgaa gcgatgaacc tcggtgatag ctgatgcgct agtaccgctg 360

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 420

361 gtgtgggcag gacacctata ggcctatatc aaggaggaaa gtcgtttttt ggggagttct 420

.......... ......... tet(variant) T7_term ......... ..........

.......... .......... .......... .......... .......... ..........

421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 480

421 gggcaaatct ccggggttcc ccaatacgat caataacgag tcgccaccgt cgtcggttga 480

.......... .. T7_term tet(variant) T7_Terminal_pri ORF 2 ..

.......... .......... BamHI(510) ..... SacII(517),KpnI(522) .....

481 cagcttcctt tcgggctttg ttagcagccg gatccgcggt accagcacca ccagcggtga 540

481 gtcgaaggaa agcccgaaac aatcgtcggc ctaggcgcca tggtcgtggt ggtcgccact 540

...... T7_term tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggtgcggaac ttctacaacc tcaaagccca taacgttgcg gatagaaccc ttctcagggt 600

541 ccacgccttg aagatgttgg agtttcgggt attgcaacgc ctatcttggg aagagtccca 600

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 caatcagagc agcgtagttt gctgcgttcg gcatcagtgc tgccagaatc gcagagtagc 660

601 gttagtctcg tcgcatcaaa cgacgcaagc cgtagtcacg acggtcttag cgtctcatcg 660

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tatctgggtc acagtagaac acacggtcag cagccggaac atagttcttg gtcagagccg 720

661 atagacccag tgtcatcttg tgtgccagtc gtcggccttg tatcaagaac cagtctcggc 720

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

721 cacgagcctt agtcagagcc gcaataatct ccttacccag cgcaacttgg tcggtaagtg 780

721 gtgctcggaa tcagtctcgg cgttattaga ggaatgggtc gcgttgaacc agccattcac 780

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cggccttgtt ctgagtggtc tcaattacgg tagcagtacc taagccctcg atgttctcat 840

781 gccggaacaa gactcaccag agttaatgcc atcgtcatgg attcgggagc tacaagagta 840

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tatatttgct ttccacgtta cacagaccgg caatctcagc cagaaccgca ccatccgcag 900

841 atataaacga aaggtgcaat gtgtctggcc gttagagtcg gtcttggcgt ggtaggcgtc 900

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ccatcgccag agattcaccc aactgagagg tatactcaga gcgaacgtcg tagtggttca 960

901 ggtagcggtc tctaagtggg ttgactctcc atatgagtct cgcttgcagc atcaccaagt 960

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tcgcgtcctc aatatcataa atcagaacgt cagccgtcag gagaccgtca atggtgatta 1020

961 agcgcaggag ttatagtatt tagtcttgca gtcggcagtc ctctggcagt taccactaat 1020

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ccttctcggt gtgtttgatg tccttacgtt tatcgtcgag gttctcgccc ggagccagat 1080

1021 ggaagagcca cacaaactac aggaatgcaa atagcagctc caagagcggg cctcggtcta 1080

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 acgctgcctg agtgcgaccc agaacaggga actgagcgga tttaccgctg gagatggaac 1140

1081 tgcgacggac tcacgctggg tcttgtccct tgactcgcct aaatggcgac ctctaccttg 1140

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtaccatgtg gcgagaagtg gtcacggagg tacgagcgaa cgcagtcagg acttcaccgc 1200

1141 catggtacac cgctcttcac cagtgcctcc atgctcgctt gcgtcagtcc tgaagtggcg 1200

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 caaatacctt caagaacaac gccagtttat ctccagcagc aactacacct ttaccttggt 1260

1201 gtttatggaa gttcttgttg cggtcaaata gaggtcgtcg ttgatgtgga aatggaacca 1260

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tagtacccat ttgctgtcca ccagtcatgc tagccatatg tatatctcct tcttaaagtt 1320

1261 atcatgggta aacgacaggt ggtcagtacg atcggtatac atatagagga agaatttcaa 1320

ORF 2 tet T7_gene10_leader T7_leader Xpress_fwd_pri T7_transl_en_RBS

.......... .......... .......... .......... .......... ..........

1321 aaacaaaatt atttctagag ggaaaccgtt gtggtctccc tatagtgagt cgtattaatt 1380

1321 tttgttttaa taaagatctc cctttggcaa caccagaggg atatcactca gcataattaa 1380

.......... ......... tet(variant) ......... T7_pro ..........

......... BglII(1392) ......... .......... .......... ..........

1381 tcgcgggatc gagatctcga tcctctacgc cggacgcatc gtggccggca tcaccggcgc 1440

1381 agcgccctag ctctagagct aggagatgcg gcctgcgtag caccggccgt agtggccgcg 1440

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cacaggtgcg gttgctggcg cctatatcgc cgacatcacc gatggggaag atcgggctcg 1500

1441 gtgtccacgc caacgaccgc ggatatagcg gctgtagtgg ctaccccttc tagcccgagc 1500

.......... .. tet(variant) pBRrevBam_pri .. .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ccacttcggg ctcatgagcg cttgtttcgg cgtgggtatg gtggcaggcc ccgtggccgg 1560

1501 ggtgaagccc gagtactcgc gaacaaagcc gcacccatac caccgtccgg ggcaccggcc 1560

.......... ......... tet(variant) ORF 2 ......... ..........

.......... .......... .......... .......... .......... ..........

1561 gggactgttg ggcgccatct ccttgcatgc accattcctt gcggcggcgg tgctcaacgg 1620

1561 ccctgacaac ccgcggtaga ggaacgtacg tggtaaggaa cgccgccgcc acgagttgcc 1620

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... SalI(1674)

1621 cctcaaccta ctactgggct gcttcctaat gcaggagtcg cataagggag agcgtcgacc 1680

1621 ggagttggat gatgacccga cgaaggatta cgtcctcagc gtattccctc tcgcagctgg 1680

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gatgcccttg agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat 1740

1681 ctacgggaac tctcggaagt tgggtcagtc gaggaaggcc acccgcgccc cgtactgata 1740

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgtcgccgca cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc 1800

1741 gcagcggcgt gaatactgac agaagaaata gtacgttgag catcctgtcc acggccgtcg 1800

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gctctgggtc attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc 1860

1801 cgagacccag taaaagccgc tcctggcgaa agcgacctcg cgctgctact agccggacag 1860

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gcttgcggta ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac 1920

1861 cgaacgccat aagccttaga acgtgcggga gcgagttcgg aagcagtgac cagggcggtg 1920

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 caaacgtttc ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta 1980

1921 gtttgcaaag ccgctcttcg tccggtaata gcggccgtac cgccggctgc gcgacccgat 1980

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... NruI(1997) .......... .......... .......... ..........

1981 cgtcttgctg gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc 2040

1981 gcagaacgac cgcaagcgct gcgctccgac ctaccggaag gggtaatact aagaagagcg 2040

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttccggcggc atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga 2100

2041 aaggccgccg tagccctacg ggcgcaacgt ccggtacgac aggtccgtcc atctactgct 2100

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccatcaggga cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg 2160

2101 ggtagtccct gtcgaagttc ctagcgagcg ccgagaatgg tcggattgaa gctagtgacc 2160

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 accgctgatc gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg 2220

2161 tggcgactag cagtgccgct aaatacggcg gagccgctcg tgtaccttgc ccaaccgtac 2220

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gattgtaggc gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag 2280

2221 ctaacatccg cggcgggata tggaacagac ggaggggcgc aacgcagcgc cacgtacctc 2280

.......... ...... tet(variant) ORF 2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccgggccacc tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca 2340

2281 ggcccggtgg agctggactt accttcggcc gccgtggagc gattgcctaa gtggtgaggt 2340

tet(variant) ORF 2 .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 agaattggag ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag 2400

2341 tcttaacctc ggttagttaa gaacgcctct tgacacttac gcgtttggtt gggaaccgtc 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aacatatcca tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt 2460

2401 ttgtataggt agcgcaggcg gtagaggtcg tcggcgtgcg ccgcgtagag cccgtcgcaa 2460

.......... .......... .......... .......... .......... ..........

MscI(2469) .......... .......... .......... .......... ..........

2461 gggtcctggc cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg 2520

2461 cccaggaccg gtgcccacgc gtactagcac gaggacagca actcctgggc cgatccgacc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cggggttgcc ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac 2580

2521 gccccaacgg aatgaccaat cgtcttactt agtggctatg cgctcgcttg cacttcgctg 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tgctgctgca aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt 2640

2581 acgacgacgt tttgcagacg ctggactcgt tgttgtactt accagaagcc aaaggcacaa 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tcgtaaagtc tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc 2700

2641 agcatttcag acctttgcgc cttcagtcgc gggacgtggt aatacaaggc ctagacgtag 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcaggatgct gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat 2760

2701 cgtcctacga cgaccgatgg gacaccttgt ggatgtagac ataattgctt cgcgaccgta 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgaccctgag tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca 2820

2761 actgggactc actaaaaaga gaccagggcg gcgtaggtat ggcggtcaac aaatgggagt 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 caacgttcca gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct 2880

2821 gttgcaaggt cattggcccg tacaagtagt agtcattggg catagcactc gtaggagaga 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cgtttcatcg gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg 2940

2881 gcaaagtagc catagtaatg ggggtacttg tctttagggg gaatgtgcct ccgtagtcac 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 accaaacagg aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg 3000

2941 tggtttgtcc ttttttggcg ggaattgtac cgggcgaaat agtcttcggt ctgtaattgc 3000

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cttctggaga aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt 3060

3001 gaagacctct ttgagttgct cgacctgcgc ctacttgtcc gtctgtagac acttagcgaa 3060

.......... .......... ROP .......... .......... ..........

.......... ......... PvuII(3089) ......... .......... ..........

3061 cacgaccacg ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa 3120

3061 gtgctggtgc gactactcga aatggcgtcg acggagcgcg caaagccact actgccactt 3120

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 3180

3121 ttggagactg tgtacgtcga gggcctctgc cagtgtcgaa cagacattcg cctacggccc 3180

ROP pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg 3240

3181 tcgtctgttc gggcagtccc gcgcagtcgc ccacaaccgc ccacagcccc gcgtcggtac 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 acccagtcac gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga 3300

3241 tgggtcagtg catcgctatc gcctcacata tgaccgaatt gatacgccgt agtctcgtct 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttgtactgag agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa 3360

3301 aacatgactc tcacgtggta tatacgccac actttatggc gtgtctacgc attcctcttt 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg 3420

3361 tatggcgtag tccgcgagaa ggcgaaggag cgagtgactg agcgacgcga gccagcaagc 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg 3480

3421 cgacgccgct cgccatagtc gagtgagttt ccgccattat gccaataggt gtcttagtcc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa 3540

3481 cctattgcgt cctttcttgt acactcgttt tccggtcgtt ttccggtcct tggcattttt 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg 3600

3541 ccggcgcaac gaccgcaaaa aggtatccga ggcgggggga ctgctcgtag tgtttttagc 3600

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3601 acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc 3660

3601 tgcgagttca gtctccaccg ctttgggctg tcctgatatt tctatggtcc gcaaaggggg 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc 3720

3661 accttcgagg gagcacgcga gaggacaagg ctgggacggc gaatggccta tggacaggcg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc 3780

3721 gaaagaggga agcccttcgc accgcgaaag agtatcgagt gcgacatcca tagagtcaag 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg 3840

3781 ccacatccag caagcgaggt tcgacccgac acacgtgctt ggggggcaag tcgggctggc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc 3900

3841 gacgcggaat aggccattga tagcagaact caggttgggc cattctgtgc tgaatagcgg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga 3960

3901 tgaccgtcgt cggtgaccat tgtcctaatc gtctcgctcc atacatccgc cacgatgtct 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc 4020

3961 caagaacttc accaccggat tgatgccgat gtgatcttcc tgtcataaac catagacgcg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac 4080

4021 agacgacttc ggtcaatgga agcctttttc tcaaccatcg agaactaggc cgtttgtttg 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg 4140

4081 gtggcgacca tcgccaccaa aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc 4200

4141 tagagttctt ctaggaaact agaaaagatg ccccagactg cgagtcacct tgcttttgag 4200

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa 4260

4201 tgcaattccc taaaaccagt actctaatag tttttcctag aagtggatct aggaaaattt 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta 4320

4261 aatttttact tcaaaattta gttagatttc atatatactc atttgaacca gactgtcaat 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt 4380

4321 ggttacgaat tagtcactcc gtggatagag tcgctagaca gataaagcaa gtaggtatca 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag 4440

4381 acggactgag gggcagcaca tctattgatg ctatgccctc ccgaatggta gaccggggtc 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag caataaacca 4500

4441 acgacgttac tatggcgctc tgggtgcgag tggccgaggt ctaaatagtc gttatttggt 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc 4560

4501 cggtcggcct tcccggctcg cgtcttcacc aggacgttga aataggcgga ggtaggtcag 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt 4620

4561 ataattaaca acggcccttc gatctcattc atcaagcggt caattatcaa acgcgttgca 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... PstI(4636) .......... .......... .......... ..........

4621 tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag 4680

4621 acaacggtaa cgacgtccgt agcaccacag tgcgagcagc aaaccatacc gaagtaagtc 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt 4740

4681 gaggccaagg gttgctagtt ccgctcaatg tactaggggg tacaacacgt tttttcgcca 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat 4800

4741 atcgaggaag ccaggaggct agcaacagtc ttcattcaac cggcgtcaca atagtgagta 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt 4860

4801 ccaataccgt cgtgacgtat taagagaatg acagtacggt aggcattcta cgaaaagaca 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc 4920

4861 ctgaccactc atgagttggt tcagtaagac tcttatcaca tacgccgctg gctcaacgag 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat 4980

4921 aacgggccgc agttgtgccc tattatggcg cggtgtatcg tcttgaaatt ttcacgagta 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag 5040

4981 gtaacctttt gcaagaagcc ccgcttttga gagttcctag aatggcgaca actctaggtc 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt 5100

5041 aagctacatt gggtgagcac gtgggttgac tagaagtcgt agaaaatgaa agtggtcgca 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg 5160

5101 aagacccact cgtttttgtc cttccgtttt acggcgtttt ttcccttatt cccgctgtgc 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta 5220

5161 ctttacaact tatgagtatg agaaggaaaa agttataata acttcgtaaa tagtcccaat 5220

ORF 1 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc 5280

5221 aacagagtac tcgcctatgt ataaacttac ataaatcttt ttatttgttt atccccaagg 5280

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt 5340

5281 cgcgtgtaaa ggggcttttc acggtggact gcagattctt tggtaataat agtactgtaa 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... EcoRI(5384) .........

5341 aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaa.... .......... 5386

5341 ttggatattt ttatccgcat agtgctccgg gaaagcagaa gttctt.... .......... 5386

.......... .......... .......... .......... .......... ..........