vtBvtB&LOCUS pET3xc 5385 bp DNA SYN 20-Sep-2007 DEFINITION pET3xc ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5385 /organism="pET3xc" /mol_type="other DNA" terminator complement(379..507) /label="T7_terminator" misc_feature 447..465 /label="T7_Terminal_primer" CDS complement(448..1296) /label="ORF frame 1" /translation="MASMTGGQQMGTNQGKGVVAAGDKLALFLKVFGGEVLTAFARTS VTTSRHMVRSISSGKSAQFPVLGRTQAAYLAPGENLDDKRKDIKHTEKVITIDGLLTA DVLIYDIEDAMNHYDVRSEYTSQLGESLAMAADGAVLAEIAGLCNVESKYNENIEGLG TATVIETTQNKAALTDQVALGKEIIAALTKARAALTKNYVPAADRVFYCDPDSYSAIL AALMPNAANYAALIDPEKGSIRNVMGFEVVEVPHLTAGGAGTGIRLLTKPERKLSWLL PPLSNN*" gene complement(86..2298) /label="tet(variant)" /gene="tet(variant)" misc_feature complement(1264..1296) /label="T7_gene10_leader" misc_feature complement(1264..1296) /label="T7_leader" misc_feature complement(1279..1297) /label="Xpress_fwd_primer" misc_feature complement(1305..1321) /label="T7_transl_en_RBS" promoter complement(1358..1376) /label="T7_promoter" misc_feature complement(1460..1479) /label="pBRrevBam_primer" CDS 1513..2298 /label="ORF frame 1" /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF LMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFG EDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLA FATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPL IVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*" misc_feature 2937..3128 /label="ROP" misc_feature complement(3122..3144) /label="pGEX_3_primer" rep_origin complement(3543..4162) /label="pBR322_origin" CDS complement(4317..5177) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4317..5177) /label="Ampicillin" /gene="Ampicillin" promoter complement(5219..5247) /label="AmpR_promoter" ORIGIN 1 TTCTCATGTT TGACAGCTTA TCATCGATAA GCTTTAATGC GGTAGTTTAT CACAGTTAAA 61 TTGCTAACGC AGTCAGGCAC CGTGTATGAA ATCTAACAAT GCGCTCATCG TCATCCTCGG 121 CACCGTCACC CTGGATGCTG TAGGCATAGG CTTGGTTATG CCGGTACTGC CGGGCCTCTT 181 GCGGGATATC GTCCATTCCG ACAGCATCGC CAGTCACTAT GGCGTGCTGC TAGCGCTATA 241 TGCGTTGATG CAATTTCTAT GCGCACCCGT TCTCGGAGCA CTGTCCGACC GCTTTGGCCG 301 CCGCCCAGTC CTGCTCGCTT CGCTACTTGG AGCCACTATC GACTACGCGA TCATGGCGAC 361 CACACCCGTC CTGTGGATAT CCGGATATAG TTCCTCCTTT CAGCAAAAAA CCCCTCAAGA 421 CCCGTTTAGA GGCCCCAAGG GGTTATGCTA GTTATTGCTC AGCGGTGGCA GCAGCCAACT 481 CAGCTTCCTT TCGGGCTTTG TTAGCAGCCG GATCCCGGTA CCAGCACCAC CAGCGGTGAG 541 GTGCGGAACT TCTACAACCT CAAAGCCCAT AACGTTGCGG ATAGAACCCT TCTCAGGGTC 601 AATCAGAGCA GCGTAGTTTG CTGCGTTCGG CATCAGTGCT GCCAGAATCG CAGAGTAGCT 661 ATCTGGGTCA CAGTAGAACA CACGGTCAGC AGCCGGAACA TAGTTCTTGG TCAGAGCCGC 721 ACGAGCCTTA GTCAGAGCCG CAATAATCTC CTTACCCAGC GCAACTTGGT CGGTAAGTGC 781 GGCCTTGTTC TGAGTGGTCT CAATTACGGT AGCAGTACCT AAGCCCTCGA TGTTCTCATT 841 ATATTTGCTT TCCACGTTAC ACAGACCGGC AATCTCAGCC AGAACCGCAC CATCCGCAGC 901 CATCGCCAGA GATTCACCCA ACTGAGAGGT ATACTCAGAG CGAACGTCGT AGTGGTTCAT 961 CGCGTCCTCA ATATCATAAA TCAGAACGTC AGCCGTCAGG AGACCGTCAA TGGTGATTAC 1021 CTTCTCGGTG TGTTTGATGT CCTTACGTTT ATCGTCGAGG TTCTCGCCCG GAGCCAGATA 1081 CGCTGCCTGA GTGCGACCCA GAACAGGGAA CTGAGCGGAT TTACCGCTGG AGATGGAACG 1141 TACCATGTGG CGAGAAGTGG TCACGGAGGT ACGAGCGAAC GCAGTCAGGA CTTCACCGCC 1201 AAATACCTTC AAGAACAACG CCAGTTTATC TCCAGCAGCA ACTACACCTT TACCTTGGTT 1261 AGTACCCATT TGCTGTCCAC CAGTCATGCT AGCCATATGT ATATCTCCTT CTTAAAGTTA 1321 AACAAAATTA TTTCTAGAGG GAAACCGTTG TGGTCTCCCT ATAGTGAGTC GTATTAATTT 1381 CGCGGGATCG AGATCTCGAT CCTCTACGCC GGACGCATCG TGGCCGGCAT CACCGGCGCC 1441 ACAGGTGCGG TTGCTGGCGC CTATATCGCC GACATCACCG ATGGGGAAGA TCGGGCTCGC 1501 CACTTCGGGC TCATGAGCGC TTGTTTCGGC GTGGGTATGG TGGCAGGCCC CGTGGCCGGG 1561 GGACTGTTGG GCGCCATCTC CTTGCATGCA CCATTCCTTG CGGCGGCGGT GCTCAACGGC 1621 CTCAACCTAC TACTGGGCTG CTTCCTAATG CAGGAGTCGC ATAAGGGAGA GCGTCGACCG 1681 ATGCCCTTGA GAGCCTTCAA CCCAGTCAGC TCCTTCCGGT GGGCGCGGGG CATGACTATC 1741 GTCGCCGCAC TTATGACTGT CTTCTTTATC ATGCAACTCG TAGGACAGGT GCCGGCAGCG 1801 CTCTGGGTCA TTTTCGGCGA GGACCGCTTT CGCTGGAGCG CGACGATGAT CGGCCTGTCG 1861 CTTGCGGTAT TCGGAATCTT GCACGCCCTC GCTCAAGCCT TCGTCACTGG TCCCGCCACC 1921 AAACGTTTCG GCGAGAAGCA GGCCATTATC GCCGGCATGG CGGCCGACGC GCTGGGCTAC 1981 GTCTTGCTGG CGTTCGCGAC GCGAGGCTGG ATGGCCTTCC CCATTATGAT TCTTCTCGCT 2041 TCCGGCGGCA TCGGGATGCC CGCGTTGCAG GCCATGCTGT CCAGGCAGGT AGATGACGAC 2101 CATCAGGGAC AGCTTCAAGG ATCGCTCGCG GCTCTTACCA GCCTAACTTC GATCACTGGA 2161 CCGCTGATCG TCACGGCGAT TTATGCCGCC TCGGCGAGCA CATGGAACGG GTTGGCATGG 2221 ATTGTAGGCG CCGCCCTATA CCTTGTCTGC CTCCCCGCGT TGCGTCGCGG TGCATGGAGC 2281 CGGGCCACCT CGACCTGAAT GGAAGCCGGC GGCACCTCGC TAACGGATTC ACCACTCCAA 2341 GAATTGGAGC CAATCAATTC TTGCGGAGAA CTGTGAATGC GCAAACCAAC CCTTGGCAGA 2401 ACATATCCAT CGCGTCCGCC ATCTCCAGCA GCCGCACGCG GCGCATCTCG GGCAGCGTTG 2461 GGTCCTGGCC ACGGGTGCGC ATGATCGTGC TCCTGTCGTT GAGGACCCGG CTAGGCTGGC 2521 GGGGTTGCCT TACTGGTTAG CAGAATGAAT CACCGATACG CGAGCGAACG TGAAGCGACT 2581 GCTGCTGCAA AACGTCTGCG ACCTGAGCAA CAACATGAAT GGTCTTCGGT TTCCGTGTTT 2641 CGTAAAGTCT GGAAACGCGG AAGTCAGCGC CCTGCACCAT TATGTTCCGG ATCTGCATCG 2701 CAGGATGCTG CTGGCTACCC TGTGGAACAC CTACATCTGT ATTAACGAAG CGCTGGCATT 2761 GACCCTGAGT GATTTTTCTC TGGTCCCGCC GCATCCATAC CGCCAGTTGT TTACCCTCAC 2821 AACGTTCCAG TAACCGGGCA TGTTCATCAT CAGTAACCCG TATCGTGAGC ATCCTCTCTC 2881 GTTTCATCGG TATCATTACC CCCATGAACA GAAATCCCCC TTACACGGAG GCATCAGTGA 2941 CCAAACAGGA AAAAACCGCC CTTAACATGG CCCGCTTTAT CAGAAGCCAG ACATTAACGC 3001 TTCTGGAGAA ACTCAACGAG CTGGACGCGG ATGAACAGGC AGACATCTGT GAATCGCTTC 3061 ACGACCACGC TGATGAGCTT TACCGCAGCT GCCTCGCGCG TTTCGGTGAT GACGGTGAAA 3121 ACCTCTGACA CATGCAGCTC CCGGAGACGG TCACAGCTTG TCTGTAAGCG GATGCCGGGA 3181 GCAGACAAGC CCGTCAGGGC GCGTCAGCGG GTGTTGGCGG GTGTCGGGGC GCAGCCATGA 3241 CCCAGTCACG TAGCGATAGC GGAGTGTATA CTGGCTTAAC TATGCGGCAT CAGAGCAGAT 3301 TGTACTGAGA GTGCACCATA TATGCGGTGT GAAATACCGC ACAGATGCGT AAGGAGAAAA 3361 TACCGCATCA GGCGCTCTTC CGCTTCCTCG CTCACTGACT CGCTGCGCTC GGTCGTTCGG 3421 CTGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG 3481 GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG 3541 GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA 3601 CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT 3661 GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC 3721 TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG 3781 GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC 3841 TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA 3901 CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG 3961 TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA CAGTATTTGG TATCTGCGCT 4021 CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC 4081 ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA 4141 TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA 4201 CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT 4261 TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AAACTTGGTC TGACAGTTAC 4321 CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC TATTTCGTTC ATCCATAGTT 4381 GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG GCTTACCATC TGGCCCCAGT 4441 GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG ATTTATCAGC AATAAACCAG 4501 CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT TATCCGCCTC CATCCAGTCT 4561 ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG TTAATAGTTT GCGCAACGTT 4621 GTTGCCATTG CTGCAGGCAT CGTGGTGTCA CGCTCGTCGT TTGGTATGGC TTCATTCAGC 4681 TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA TGTTGTGCAA AAAAGCGGTT 4741 AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG CCGCAGTGTT ATCACTCATG 4801 GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT CCGTAAGATG CTTTTCTGTG 4861 ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA TGCGGCGACC GAGTTGCTCT 4921 TGCCCGGCGT CAACACGGGA TAATACCGCG CCACATAGCA GAACTTTAAA AGTGCTCATC 4981 ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT TACCGCTGTT GAGATCCAGT 5041 TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT CTTTTACTTT CACCAGCGTT 5101 TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA AGGGAATAAG GGCGACACGG 5161 AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 5221 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 5281 CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA CCATTATTAT CATGACATTA 5341 ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTTC AAGAA // pET3xc "Times New RomanvtBC {T7_term"Times New RomanvtB V tet(variant)"Times New RomanvtBC T7_Terminal_pri"Times New Roman)vtBCɆ ORF 1"Times New Roman<vtBC T7_gene10_leader"Times New RomanvtBC  T7_leader"Times New Roman)vtBCɆ Xpress_fwd_pri"Times New RomanQvtB )T7_transl_en_RBS"Times New RomanvtBC N`T7_pro"Times New RomanvtBC  pBRrevBam_pri"Times New RomanvtBCɆ ORF 1"Times New RomanvtB y 8 ROP"Times New RomanvtBC 2 H  pGEX_3_pri"Times New RomanvtBC  B pBR322_origin"Times New RomanvtBCɆ 9ORF 3"Times New RomanvtB 9 Ampicillin"Times New RomanvtBC cAmpR_pro"Times New Roman vtBClaI(24),HindIII(29)"Times New Roman vbWHf vtBBamHI(510),KpnI(521)"Times New Roman ${$]k{ vtB!NdeI(1295),XbaI(1333),BglII(1391)"Times New Roman sKJH2^ vtBSalI"Times New Roman dw vtBEagI(1961),NruI(1996)"Times New Roman ;JY Y + vtBMscI"Times New Roman  9L__n vtBPvuII"Times New Roman  )* <MM8\ vtBPstI"Times New Roman  vtBAatII(5312),EcoRI(5383)"Times New Roman yv`vN-0vN vtBtest"Times New Roman N  vtBtest"Times New Roman` s`QcEbE