Sequence of pET3xc

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Sequence of pET3xc

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Sequence of pET3xc : 5385 BP; 1237 A; 1487 C; 1379 G; 1282 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... ......... tet(variant) .........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata 240

181 cgccctatag caggtaaggc tgtcgtagcg gtcagtgata ccgcacgacg atcgcgatat 240

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg 300

241 acgcaactac gttaaagata cgcgtgggca agagcctcgt gacaggctgg cgaaaccggc 300

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac 360

301 ggcgggtcag gacgagcgaa gcgatgaacc tcggtgatag ctgatgcgct agtaccgctg 360

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga 420

361 gtgtgggcag gacacctata ggcctatatc aaggaggaaa gtcgtttttt ggggagttct 420

.......... ......... tet(variant) T7_term ......... ..........

.......... .......... .......... .......... .......... ..........

421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact 480

421 gggcaaatct ccggggttcc ccaatacgat caataacgag tcgccaccgt cgtcggttga 480

.......... .. T7_term tet(variant) T7_Terminal_pri ORF 1 ..

.......... .......... BamHI(510) .......... KpnI(521) ..........

481 cagcttcctt tcgggctttg ttagcagccg gatcccggta ccagcaccac cagcggtgag 540

481 gtcgaaggaa agcccgaaac aatcgtcggc ctagggccat ggtcgtggtg gtcgccactc 540

...... T7_term tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gtgcggaact tctacaacct caaagcccat aacgttgcgg atagaaccct tctcagggtc 600

541 cacgccttga agatgttgga gtttcgggta ttgcaacgcc tatcttggga agagtcccag 600

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 aatcagagca gcgtagtttg ctgcgttcgg catcagtgct gccagaatcg cagagtagct 660

601 ttagtctcgt cgcatcaaac gacgcaagcc gtagtcacga cggtcttagc gtctcatcga 660

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 atctgggtca cagtagaaca cacggtcagc agccggaaca tagttcttgg tcagagccgc 720

661 tagacccagt gtcatcttgt gtgccagtcg tcggccttgt atcaagaacc agtctcggcg 720

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

721 acgagcctta gtcagagccg caataatctc cttacccagc gcaacttggt cggtaagtgc 780

721 tgctcggaat cagtctcggc gttattagag gaatgggtcg cgttgaacca gccattcacg 780

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ggccttgttc tgagtggtct caattacggt agcagtacct aagccctcga tgttctcatt 840

781 ccggaacaag actcaccaga gttaatgcca tcgtcatgga ttcgggagct acaagagtaa 840

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

841 atatttgctt tccacgttac acagaccggc aatctcagcc agaaccgcac catccgcagc 900

841 tataaacgaa aggtgcaatg tgtctggccg ttagagtcgg tcttggcgtg gtaggcgtcg 900

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

901 catcgccaga gattcaccca actgagaggt atactcagag cgaacgtcgt agtggttcat 960

901 gtagcggtct ctaagtgggt tgactctcca tatgagtctc gcttgcagca tcaccaagta 960

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cgcgtcctca atatcataaa tcagaacgtc agccgtcagg agaccgtcaa tggtgattac 1020

961 gcgcaggagt tatagtattt agtcttgcag tcggcagtcc tctggcagtt accactaatg 1020

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 cttctcggtg tgtttgatgt ccttacgttt atcgtcgagg ttctcgcccg gagccagata 1080

1021 gaagagccac acaaactaca ggaatgcaaa tagcagctcc aagagcgggc ctcggtctat 1080

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cgctgcctga gtgcgaccca gaacagggaa ctgagcggat ttaccgctgg agatggaacg 1140

1081 gcgacggact cacgctgggt cttgtccctt gactcgccta aatggcgacc tctaccttgc 1140

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 taccatgtgg cgagaagtgg tcacggaggt acgagcgaac gcagtcagga cttcaccgcc 1200

1141 atggtacacc gctcttcacc agtgcctcca tgctcgcttg cgtcagtcct gaagtggcgg 1200

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aaataccttc aagaacaacg ccagtttatc tccagcagca actacacctt taccttggtt 1260

1201 tttatggaag ttcttgttgc ggtcaaatag aggtcgtcgt tgatgtggaa atggaaccaa 1260

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 agtacccatt tgctgtccac cagtcatgct agccatatgt atatctcctt cttaaagtta 1320

1261 tcatgggtaa acgacaggtg gtcagtacga tcggtataca tatagaggaa gaatttcaat 1320

ORF 1 T7_gene10_leader T7_leader tet Xpress_fwd_pri T7_transl_en_RBS

.......... .......... .......... .......... .......... ..........

1321 aacaaaatta tttctagagg gaaaccgttg tggtctccct atagtgagtc gtattaattt 1380

1321 ttgttttaat aaagatctcc ctttggcaac accagaggga tatcactcag cataattaaa 1380

.......... ......... tet(variant) ......... T7_pro ..........

......... BglII(1391) ......... .......... .......... ..........

1381 cgcgggatcg agatctcgat cctctacgcc ggacgcatcg tggccggcat caccggcgcc 1440

1381 gcgccctagc tctagagcta ggagatgcgg cctgcgtagc accggccgta gtggccgcgg 1440

.......... ......... tet(variant) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc 1500

1441 tgtccacgcc aacgaccgcg gatatagcgg ctgtagtggc taccccttct agcccgagcg 1500

.......... .. tet(variant) pBRrevBam_pri .. .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg 1560

1501 gtgaagcccg agtactcgcg aacaaagccg cacccatacc accgtccggg gcaccggccc 1560

.......... ......... tet(variant) ORF 1 ......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggactgttgg gcgccatctc cttgcatgca ccattccttg cggcggcggt gctcaacggc 1620

1561 cctgacaacc cgcggtagag gaacgtacgt ggtaaggaac gccgccgcca cgagttgccg 1620

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... SalI(1673)

1621 ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga gcgtcgaccg 1680

1621 gagttggatg atgacccgac gaaggattac gtcctcagcg tattccctct cgcagctggc 1680

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc 1740

1681 tacgggaact ctcggaagtt gggtcagtcg aggaaggcca cccgcgcccc gtactgatag 1740

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg 1800

1741 cagcggcgtg aatactgaca gaagaaatag tacgttgagc atcctgtcca cggccgtcgc 1800

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg 1860

1801 gagacccagt aaaagccgct cctggcgaaa gcgacctcgc gctgctacta gccggacagc 1860

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc 1920

1861 gaacgccata agccttagaa cgtgcgggag cgagttcgga agcagtgacc agggcggtgg 1920

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac 1980

1921 tttgcaaagc cgctcttcgt ccggtaatag cggccgtacc gccggctgcg cgacccgatg 1980

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... NruI(1996) .......... .......... .......... ..........

1981 gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct 2040

1981 cagaacgacc gcaagcgctg cgctccgacc taccggaagg ggtaatacta agaagagcga 2040

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac 2100

2041 aggccgccgt agccctacgg gcgcaacgtc cggtacgaca ggtccgtcca tctactgctg 2100

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga 2160

2101 gtagtccctg tcgaagttcc tagcgagcgc cgagaatggt cggattgaag ctagtgacct 2160

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg 2220

2161 ggcgactagc agtgccgcta aatacggcgg agccgctcgt gtaccttgcc caaccgtacc 2220

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 attgtaggcg ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc 2280

2221 taacatccgc ggcgggatat ggaacagacg gaggggcgca acgcagcgcc acgtacctcg 2280

.......... ...... tet(variant) ORF 1 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgggccacct cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa 2340

2281 gcccggtgga gctggactta ccttcggccg ccgtggagcg attgcctaag tggtgaggtt 2340

tet(variant) ORF 1 .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gaattggagc caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga 2400

2341 cttaacctcg gttagttaag aacgcctctt gacacttacg cgtttggttg ggaaccgtct 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 acatatccat cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg 2460

2401 tgtataggta gcgcaggcgg tagaggtcgt cggcgtgcgc cgcgtagagc ccgtcgcaac 2460

.......... .......... .......... .......... .......... ..........

MscI(2468) .......... .......... .......... .......... ..........

2461 ggtcctggcc acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc 2520

2461 ccaggaccgg tgcccacgcg tactagcacg aggacagcaa ctcctgggcc gatccgaccg 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggggttgcct tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact 2580

2521 ccccaacgga atgaccaatc gtcttactta gtggctatgc gctcgcttgc acttcgctga 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gctgctgcaa aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt 2640

2581 cgacgacgtt ttgcagacgc tggactcgtt gttgtactta ccagaagcca aaggcacaaa 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgtaaagtct ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg 2700

2641 gcatttcaga cctttgcgcc ttcagtcgcg ggacgtggta atacaaggcc tagacgtagc 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt 2760

2701 gtcctacgac gaccgatggg acaccttgtg gatgtagaca taattgcttc gcgaccgtaa 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac 2820

2761 ctgggactca ctaaaaagag accagggcgg cgtaggtatg gcggtcaaca aatgggagtg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc 2880

2821 ttgcaaggtc attggcccgt acaagtagta gtcattgggc atagcactcg taggagagag 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gtttcatcgg tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga 2940

2881 caaagtagcc atagtaatgg gggtacttgt ctttaggggg aatgtgcctc cgtagtcact 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ccaaacagga aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc 3000

2941 ggtttgtcct tttttggcgg gaattgtacc gggcgaaata gtcttcggtc tgtaattgcg 3000

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttctggagaa actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc 3060

3001 aagacctctt tgagttgctc gacctgcgcc tacttgtccg tctgtagaca cttagcgaag 3060

.......... .......... ROP .......... .......... ..........

.......... ......... PvuII(3088) ......... .......... ..........

3061 acgaccacgc tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa 3120

3061 tgctggtgcg actactcgaa atggcgtcga cggagcgcgc aaagccacta ctgccacttt 3120

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga 3180

3121 tggagactgt gtacgtcgag ggcctctgcc agtgtcgaac agacattcgc ctacggccct 3180

.......... pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga 3240

3181 cgtctgttcg ggcagtcccg cgcagtcgcc cacaaccgcc cacagccccg cgtcggtact 3240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 cccagtcacg tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat 3300

3241 gggtcagtgc atcgctatcg cctcacatat gaccgaattg atacgccgta gtctcgtcta 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tgtactgaga gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa 3360

3301 acatgactct cacgtggtat atacgccaca ctttatggcg tgtctacgca ttcctctttt 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg 3420

3361 atggcgtagt ccgcgagaag gcgaaggagc gagtgactga gcgacgcgag ccagcaagcc 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg 3480

3421 gacgccgctc gccatagtcg agtgagtttc cgccattatg ccaataggtg tcttagtccc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag 3540

3481 ctattgcgtc ctttcttgta cactcgtttt ccggtcgttt tccggtcctt ggcatttttc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga 3600

3541 cggcgcaacg accgcaaaaa ggtatccgag gcggggggac tgctcgtagt gtttttagct 3600

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3601 cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct 3660

3601 gcgagttcag tctccaccgc tttgggctgt cctgatattt ctatggtccg caaaggggga 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc 3720

3661 ccttcgaggg agcacgcgag aggacaaggc tgggacggcg aatggcctat ggacaggcgg 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg 3780

3721 aaagagggaa gcccttcgca ccgcgaaaga gtatcgagtg cgacatccat agagtcaagc 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc 3840

3781 cacatccagc aagcgaggtt cgacccgaca cacgtgcttg gggggcaagt cgggctggcg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca 3900

3841 acgcggaata ggccattgat agcagaactc aggttgggcc attctgtgct gaatagcggt 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag 3960

3901 gaccgtcgtc ggtgaccatt gtcctaatcg tctcgctcca tacatccgcc acgatgtctc 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct 4020

3961 aagaacttca ccaccggatt gatgccgatg tgatcttcct gtcataaacc atagacgcga 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc 4080

4021 gacgacttcg gtcaatggaa gcctttttct caaccatcga gaactaggcc gtttgtttgg 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga 4140

4081 tggcgaccat cgccaccaaa aaaacaaacg ttcgtcgtct aatgcgcgtc tttttttcct 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca 4200

4141 agagttcttc taggaaacta gaaaagatgc cccagactgc gagtcacctt gcttttgagt 4200

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat 4260

4201 gcaattccct aaaaccagta ctctaatagt ttttcctaga agtggatcta ggaaaattta 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac 4320

4261 atttttactt caaaatttag ttagatttca tatatactca tttgaaccag actgtcaatg 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt 4380

4321 gttacgaatt agtcactccg tggatagagt cgctagacag ataaagcaag taggtatcaa 4380

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt 4440

4381 cggactgagg ggcagcacat ctattgatgc tatgccctcc cgaatggtag accggggtca 4440

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag 4500

4441 cgacgttact atggcgctct gggtgcgagt ggccgaggtc taaatagtcg ttatttggtc 4500

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct 4560

4501 ggtcggcctt cccggctcgc gtcttcacca ggacgttgaa ataggcggag gtaggtcaga 4560

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt 4620

4561 taattaacaa cggcccttcg atctcattca tcaagcggtc aattatcaaa cgcgttgcaa 4620

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... PstI(4635) .......... .......... .......... ..........

4621 gttgccattg ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc 4680

4621 caacggtaac gacgtccgta gcaccacagt gcgagcagca aaccataccg aagtaagtcg 4680

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt 4740

4681 aggccaaggg ttgctagttc cgctcaatgt actagggggt acaacacgtt ttttcgccaa 4740

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg 4800

4741 tcgaggaagc caggaggcta gcaacagtct tcattcaacc ggcgtcacaa tagtgagtac 4800

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg 4860

4801 caataccgtc gtgacgtatt aagagaatga cagtacggta ggcattctac gaaaagacac 4860

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct 4920

4861 tgaccactca tgagttggtt cagtaagact cttatcacat acgccgctgg ctcaacgaga 4920

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgcccggcgt caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc 4980

4921 acgggccgca gttgtgccct attatggcgc ggtgtatcgt cttgaaattt tcacgagtag 4980

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt 5040

4981 taaccttttg caagaagccc cgcttttgag agttcctaga atggcgacaa ctctaggtca 5040

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt 5100

5041 agctacattg ggtgagcacg tgggttgact agaagtcgta gaaaatgaaa gtggtcgcaa 5100

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg 5160

5101 agacccactc gtttttgtcc ttccgtttta cggcgttttt tcccttattc ccgctgtgcc 5160

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta tcagggttat 5220

5161 tttacaactt atgagtatga gaaggaaaaa gttataataa cttcgtaaat agtcccaata 5220

ORF 3 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg 5280

5221 acagagtact cgcctatgta taaacttaca taaatctttt tatttgttta tccccaaggc 5280

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta 5340

5281 gcgtgtaaag gggcttttca cggtggactg cagattcttt ggtaataata gtactgtaat 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... EcoRI(5383) .........

5341 acctataaaa ataggcgtat cacgaggccc tttcgtcttc aagaa..... .......... 5385

5341 tggatatttt tatccgcata gtgctccggg aaagcagaag ttctt..... .......... 5385

.......... .......... .......... .......... .......... ..........