Sequence of pET5a

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Sequence of pET5a

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Sequence of pET5a : 4134 BP; 948 A; 1132 C; 1073 G; 981 T; 0 other;

BamHI(4) .......... .......... NheI(37) .......... ..........

1 ttcggatccg cgacccattt gctgtccacc agtcatgcta gccatatgta tatctccttc 60

1 aagcctaggc gctgggtaaa cgacaggtgg tcagtacgat cggtatacat atagaggaag 60

.......... T7_gene10_leader T7_leader Xpress_fwd_pri ..........

.......... .......... .......... .......... .......... ..........

61 ttaaagttaa acaaaattat ttctagaggg aaaccgttgt ggtctcccta tagtgagtcg 120

61 aatttcaatt tgttttaata aagatctccc tttggcaaca ccagagggat atcactcagc 120

T7_transl_en_RBS .... .......... .......... .......... T7_pro

.......... BglII(140) .......... .......... .......... ..........

121 tattaatttc gcgggatcga gatctcgatc ctctacgccg gacgcatcgt ggccggcatc 180

121 ataattaaag cgccctagct ctagagctag gagatgcggc ctgcgtagca ccggccgtag 180

.......... .......... .......... ........ tet(1191-300) ........

.......... .......... .......... .......... .......... ..........

181 accggcgcca caggtgcggt tgctggcgcc tatatcgccg acatcaccga tggggaagat 240

181 tggccgcggt gtccacgcca acgaccgcgg atatagcggc tgtagtggct accccttcta 240

.......... ...... tet(1191-300) pBRrevBam_pri ...... ..........

.......... .......... .......... .......... .......... ..........

241 cgggctcgcc acttcgggct catgagcgct tgtttcggcg tgggtatggt ggcaggcccc 300

241 gcccgagcgg tgaagcccga gtactcgcga acaaagccgc acccatacca ccgtccgggg 300

.......... ........ tet(1191-300) ........ ORF 1 ..........

.......... .......... .......... .......... .......... ..........

301 gtggccgggg gactgttggg cgccatctcc ttgcatgcac cattccttgc ggcggcggtg 360

301 caccggcccc ctgacaaccc gcggtagagg aacgtacgtg gtaaggaacg ccgccgccac 360

.......... ..... tet(1191-300) ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ctcaacggcc tcaacctact actgggctgc ttcctaatgc aggagtcgca taagggagag 420

361 gagttgccgg agttggatga tgacccgacg aaggattacg tcctcagcgt attccctctc 420

.......... ..... tet(1191-300) ORF 1 ..... .......... ..........

SalI(422) .......... .......... .......... .......... ..........

421 cgtcgaccga tgcccttgag agccttcaac ccagtcagct ccttccggtg ggcgcggggc 480

421 gcagctggct acgggaactc tcggaagttg ggtcagtcga ggaaggccac ccgcgccccg 480

.......... ..... tet(1191-300) ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atgactatcg tcgccgcact tatgactgtc ttctttatca tgcaactcgt aggacaggtg 540

481 tactgatagc agcggcgtga atactgacag aagaaatagt acgttgagca tcctgtccac 540

.......... ..... tet(1191-300) ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccggcagcgc tctgggtcat tttcggcgag gaccgctttc gctggagcgc gacgatgatc 600

541 ggccgtcgcg agacccagta aaagccgctc ctggcgaaag cgacctcgcg ctgctactag 600

.......... ..... tet(1191-300) ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggcctgtcgc ttgcggtatt cggaatcttg cacgccctcg ctcaagcctt cgtcactggt 660

601 ccggacagcg aacgccataa gccttagaac gtgcgggagc gagttcggaa gcagtgacca 660

.......... ..... tet(1191-300) ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cccgccacca aacgtttcgg cgagaagcag gccattatcg ccggcatggc ggccgacgcg 720

661 gggcggtggt ttgcaaagcc gctcttcgtc cggtaatagc ggccgtaccg ccggctgcgc 720

.......... ..... tet(1191-300) ORF 1 ..... .......... ..........

.......... .......... NruI(745) .......... .......... ..........

721 ctgggctacg tcttgctggc gttcgcgacg cgaggctgga tggccttccc cattatgatt 780

721 gacccgatgc agaacgaccg caagcgctgc gctccgacct accggaaggg gtaatactaa 780

.......... ..... tet(1191-300) ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cttctcgctt ccggcggcat cgggatgccc gcgttgcagg ccatgctgtc caggcaggta 840

781 gaagagcgaa ggccgccgta gccctacggg cgcaacgtcc ggtacgacag gtccgtccat 840

.......... ..... tet(1191-300) ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

841 gatgacgacc atcagggaca gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg 900

841 ctactgctgg tagtccctgt cgaagttcct agcgagcgcc gagaatggtc ggattgaagc 900

.......... ..... tet(1191-300) ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

901 atcactggac cgctgatcgt cacggcgatt tatgccgcct cggcgagcac atggaacggg 960

901 tagtgacctg gcgactagca gtgccgctaa atacggcgga gccgctcgtg taccttgccc 960

.......... ..... tet(1191-300) ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ttggcatgga ttgtaggcgc cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt 1020

961 aaccgtacct aacatccgcg gcgggatatg gaacagacgg aggggcgcaa cgcagcgcca 1020

.......... ..... tet(1191-300) ORF 1 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gcatggagcc gggccacctc gacctgaatg gaagccggcg gcacctcgct aacggattca 1080

1021 cgtacctcgg cccggtggag ctggacttac cttcggccgc cgtggagcga ttgcctaagt 1080

..... tet(1191-300) ORF 1 ..... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ccactccaag aattggagcc aatcaattct tgcggagaac tgtgaatgcg caaaccaacc 1140

1081 ggtgaggttc ttaacctcgg ttagttaaga acgcctcttg acacttacgc gtttggttgg 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cttggcagaa catatccatc gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg 1200

1141 gaaccgtctt gtataggtag cgcaggcggt agaggtcgtc ggcgtgcgcc gcgtagagcc 1200

.......... .......... .......... .......... .......... ..........

.......... MscI(1217) .......... .......... .......... ..........

1201 gcagcgttgg gtcctggcca cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc 1260

1201 cgtcgcaacc caggaccggt gcccacgcgt actagcacga ggacagcaac tcctgggccg 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 taggctggcg gggttgcctt actggttagc agaatgaatc accgatacgc gagcgaacgt 1320

1261 atccgaccgc cccaacggaa tgaccaatcg tcttacttag tggctatgcg ctcgcttgca 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gaagcgactg ctgctgcaaa acgtctgcga cctgagcaac aacatgaatg gtcttcggtt 1380

1321 cttcgctgac gacgacgttt tgcagacgct ggactcgttg ttgtacttac cagaagccaa 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tccgtgtttc gtaaagtctg gaaacgcgga agtcagcgcc ctgcaccatt atgttccgga 1440

1381 aggcacaaag catttcagac ctttgcgcct tcagtcgcgg gacgtggtaa tacaaggcct 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tctgcatcgc aggatgctgc tggctaccct gtggaacacc tacatctgta ttaacgaagc 1500

1441 agacgtagcg tcctacgacg accgatggga caccttgtgg atgtagacat aattgcttcg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gctggcattg accctgagtg atttttctct ggtcccgccg catccatacc gccagttgtt 1560

1501 cgaccgtaac tgggactcac taaaaagaga ccagggcggc gtaggtatgg cggtcaacaa 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 taccctcaca acgttccagt aaccgggcat gttcatcatc agtaacccgt atcgtgagca 1620

1561 atgggagtgt tgcaaggtca ttggcccgta caagtagtag tcattgggca tagcactcgt 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tcctctctcg tttcatcggt atcattaccc ccatgaacag aaatccccct tacacggagg 1680

1621 aggagagagc aaagtagcca tagtaatggg ggtacttgtc tttaggggga atgtgcctcc 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 catcagtgac caaacaggaa aaaaccgccc ttaacatggc ccgctttatc agaagccaga 1740

1681 gtagtcactg gtttgtcctt ttttggcggg aattgtaccg ggcgaaatag tcttcggtct 1740

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cattaacgct tctggagaaa ctcaacgagc tggacgcgga tgaacaggca gacatctgtg 1800

1741 gtaattgcga agacctcttt gagttgctcg acctgcgcct acttgtccgt ctgtagacac 1800

.......... .......... ROP .......... .......... ..........

.......... .......... ......... PvuII(1837) ......... ..........

1801 aatcgcttca cgaccacgct gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg 1860

1801 ttagcgaagt gctggtgcga ctactcgaaa tggcgtcgac ggagcgcgca aagccactac 1860

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg 1920

1861 tgccactttt ggagactgtg tacgtcgagg gcctctgcca gtgtcgaaca gacattcgcc 1920

ROP .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg 1980

1921 tacggccctc gtctgttcgg gcagtcccgc gcagtcgccc acaaccgccc acagccccgc 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cagccatgac ccagtcacgt agcgatagcg gagtgtatac tggcttaact atgcggcatc 2040

1981 gtcggtactg ggtcagtgca tcgctatcgc ctcacatatg accgaattga tacgccgtag 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agagcagatt gtactgagag tgcaccatat atgcggtgtg aaataccgca cagatgcgta 2100

2041 tctcgtctaa catgactctc acgtggtata tacgccacac tttatggcgt gtctacgcat 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aggagaaaat accgcatcag gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg 2160

2101 tcctctttta tggcgtagtc cgcgagaagg cgaaggagcg agtgactgag cgacgcgagc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca 2220

2161 cagcaagccg acgccgctcg ccatagtcga gtgagtttcc gccattatgc caataggtgt 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac 2280

2221 cttagtcccc tattgcgtcc tttcttgtac actcgttttc cggtcgtttt ccggtccttg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac 2340

2281 gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg cggggggact gctcgtagtg 2340

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2341 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg 2400

2341 tttttagctg cgagttcagt ctccaccgct ttgggctgtc ctgatatttc tatggtccgc 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac 2460

2401 aaagggggac cttcgaggga gcacgcgaga ggacaaggct gggacggcga atggcctatg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat 2520

2461 gacaggcgga aagagggaag cccttcgcac cgcgaaagag tatcgagtgc gacatccata 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag 2580

2521 gagtcaagcc acatccagca agcgaggttc gacccgacac acgtgcttgg ggggcaagtc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac 2640

2581 gggctggcga cgcggaatag gccattgata gcagaactca ggttgggcca ttctgtgctg 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt 2700

2641 aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat acatccgcca 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt 2760

2701 cgatgtctca agaacttcac caccggattg atgccgatgt gatcttcctg tcataaacca 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc 2820

2761 tagacgcgag acgacttcgg tcaatggaag cctttttctc aaccatcgag aactaggccg 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga 2880

2821 tttgtttggt ggcgaccatc gccaccaaaa aaacaaacgt tcgtcgtcta atgcgcgtct 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac 2940

2881 ttttttccta gagttcttct aggaaactag aaaagatgcc ccagactgcg agtcaccttg 2940

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc 3000

2941 cttttgagtg caattcccta aaaccagtac tctaatagtt tttcctagaa gtggatctag 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct 3060

3001 gaaaatttaa tttttacttc aaaatttagt tagatttcat atatactcat ttgaaccaga 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca 3120

3061 ctgtcaatgg ttacgaatta gtcactccgt ggatagagtc gctagacaga taaagcaagt 3120

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3121 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct 3180

3121 aggtatcaac ggactgaggg gcagcacatc tattgatgct atgccctccc gaatggtaga 3180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca 3240

3181 ccggggtcac gacgttacta tggcgctctg ggtgcgagtg gccgaggtct aaatagtcgt 3240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc 3300

3241 tatttggtcg gtcggccttc ccggctcgcg tcttcaccag gacgttgaaa taggcggagg 3300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg 3360

3301 taggtcagat aattaacaac ggcccttcga tctcattcat caagcggtca attatcaaac 3360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... PstI(3384) .......... .......... ..........

3361 cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct 3420

3361 gcgttgcaac aacggtaacg acgtccgtag caccacagtg cgagcagcaa accataccga 3420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa 3480

3421 agtaagtcga ggccaagggt tgctagttcc gctcaatgta ctagggggta caacacgttt 3480

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta 3540

3481 tttcgccaat cgaggaagcc aggaggctag caacagtctt cattcaaccg gcgtcacaat 3540

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc 3600

3541 agtgagtacc aataccgtcg tgacgtatta agagaatgac agtacggtag gcattctacg 3600

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg 3660

3601 aaaagacact gaccactcat gagttggttc agtaagactc ttatcacata cgccgctggc 3660

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 agttgctctt gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa 3720

3661 tcaacgagaa cgggccgcag ttgtgcccta ttatggcgcg gtgtatcgtc ttgaaatttt 3720

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg 3780

3721 cacgagtagt aaccttttgc aagaagcccc gcttttgaga gttcctagaa tggcgacaac 3780

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc 3840

3781 tctaggtcaa gctacattgg gtgagcacgt gggttgacta gaagtcgtag aaaatgaaag 3840

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg 3900

3841 tggtcgcaaa gacccactcg tttttgtcct tccgttttac ggcgtttttt cccttattcc 3900

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat 3960

3901 cgctgtgcct ttacaactta tgagtatgag aaggaaaaag ttataataac ttcgtaaata 3960

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata 4020

3961 gtcccaataa cagagtactc gcctatgtat aaacttacat aaatcttttt atttgtttat 4020

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc 4080

4021 ccccaaggcg cgtgtaaagg ggcttttcac ggtggactgc agattctttg gtaataatag 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... EcoRI(4132)

4081 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa...... 4134

4081 tactgtaatt ggatattttt atccgcatag tgctccggga aagcagaagt tctt...... 4134

.......... .......... .......... .......... .......... ..........