Sequence of pET5b

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Sequence of pET5b

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Sequence of pET5b : 4133 BP; 948 A; 1132 C; 1072 G; 981 T; 0 other;

BamHI(4) .......... .......... NheI(36) .......... ..........

1 ttcggatccc gacccatttg ctgtccacca gtcatgctag ccatatgtat atctccttct 60

1 aagcctaggg ctgggtaaac gacaggtggt cagtacgatc ggtatacata tagaggaaga 60

.......... T7_gene10_leader T7_leader Xpress_fwd_pri ..........

.......... .......... .......... .......... .......... ..........

61 taaagttaaa caaaattatt tctagaggga aaccgttgtg gtctccctat agtgagtcgt 120

61 atttcaattt gttttaataa agatctccct ttggcaacac cagagggata tcactcagca 120

T7_transl_en_RBS .... .......... .......... .......... T7_pro

.......... BglII(139) .......... .......... .......... ..........

121 attaatttcg cgggatcgag atctcgatcc tctacgccgg acgcatcgtg gccggcatca 180

121 taattaaagc gccctagctc tagagctagg agatgcggcc tgcgtagcac cggccgtagt 180

.......... .......... .......... ........ tet(1191-300) ........

.......... .......... .......... .......... .......... ..........

181 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc 240

181 ggccgcggtg tccacgccaa cgaccgcgga tatagcggct gtagtggcta ccccttctag 240

.......... ...... tet(1191-300) pBRrevBam_pri ...... ..........

.......... .......... .......... .......... .......... ..........

241 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg 300

241 cccgagcggt gaagcccgag tactcgcgaa caaagccgca cccataccac cgtccggggc 300

.......... ........ tet(1191-300) ORF 3 ........ ..........

.......... .......... .......... .......... .......... ..........

301 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc 360

301 accggccccc tgacaacccg cggtagagga acgtacgtgg taaggaacgc cgccgccacg 360

.......... ..... tet(1191-300) ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

361 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc 420

361 agttgccgga gttggatgat gacccgacga aggattacgt cctcagcgta ttccctctcg 420

.......... ..... tet(1191-300) ORF 3 ..... .......... ..........

SalI(421) .......... .......... .......... .......... ..........

421 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca 480

421 cagctggcta cgggaactct cggaagttgg gtcagtcgag gaaggccacc cgcgccccgt 480

.......... ..... tet(1191-300) ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc 540

481 actgatagca gcggcgtgaa tactgacaga agaaatagta cgttgagcat cctgtccacg 540

.......... ..... tet(1191-300) ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg 600

541 gccgtcgcga gacccagtaa aagccgctcc tggcgaaagc gacctcgcgc tgctactagc 600

.......... ..... tet(1191-300) ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc 660

601 cggacagcga acgccataag ccttagaacg tgcgggagcg agttcggaag cagtgaccag 660

.......... ..... tet(1191-300) ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc 720

661 ggcggtggtt tgcaaagccg ctcttcgtcc ggtaatagcg gccgtaccgc cggctgcgcg 720

.......... ..... tet(1191-300) ORF 3 ..... .......... ..........

.......... .......... NruI(744) .......... .......... ..........

721 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc 780

721 acccgatgca gaacgaccgc aagcgctgcg ctccgaccta ccggaagggg taatactaag 780

.......... ..... tet(1191-300) ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag 840

781 aagagcgaag gccgccgtag ccctacgggc gcaacgtccg gtacgacagg tccgtccatc 840

.......... ..... tet(1191-300) ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

841 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga 900

841 tactgctggt agtccctgtc gaagttccta gcgagcgccg agaatggtcg gattgaagct 900

.......... ..... tet(1191-300) ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tcactggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt 960

901 agtgacctgg cgactagcag tgccgctaaa tacggcggag ccgctcgtgt accttgccca 960

.......... ..... tet(1191-300) ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg 1020

961 accgtaccta acatccgcgg cgggatatgg aacagacgga ggggcgcaac gcagcgccac 1020

.......... ..... tet(1191-300) ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac 1080

1021 gtacctcggc ccggtggagc tggacttacc ttcggccgcc gtggagcgat tgcctaagtg 1080

..... tet(1191-300) ORF 3 ..... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc 1140

1081 gtgaggttct taacctcggt tagttaagaa cgcctcttga cacttacgcg tttggttggg 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg 1200

1141 aaccgtcttg tataggtagc gcaggcggta gaggtcgtcg gcgtgcgccg cgtagagccc 1200

.......... .......... .......... .......... .......... ..........

.......... MscI(1216) .......... .......... .......... ..........

1201 cagcgttggg tcctggccac gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct 1260

1201 gtcgcaaccc aggaccggtg cccacgcgta ctagcacgag gacagcaact cctgggccga 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aggctggcgg ggttgcctta ctggttagca gaatgaatca ccgatacgcg agcgaacgtg 1320

1261 tccgaccgcc ccaacggaat gaccaatcgt cttacttagt ggctatgcgc tcgcttgcac 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aagcgactgc tgctgcaaaa cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt 1380

1321 ttcgctgacg acgacgtttt gcagacgctg gactcgttgt tgtacttacc agaagccaaa 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ccgtgtttcg taaagtctgg aaacgcggaa gtcagcgccc tgcaccatta tgttccggat 1440

1381 ggcacaaagc atttcagacc tttgcgcctt cagtcgcggg acgtggtaat acaaggccta 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagcg 1500

1441 gacgtagcgt cctacgacga ccgatgggac accttgtgga tgtagacata attgcttcgc 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ctggcattga ccctgagtga tttttctctg gtcccgccgc atccataccg ccagttgttt 1560

1501 gaccgtaact gggactcact aaaaagagac cagggcggcg taggtatggc ggtcaacaaa 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 accctcacaa cgttccagta accgggcatg ttcatcatca gtaacccgta tcgtgagcat 1620

1561 tgggagtgtt gcaaggtcat tggcccgtac aagtagtagt cattgggcat agcactcgta 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cctctctcgt ttcatcggta tcattacccc catgaacaga aatccccctt acacggaggc 1680

1621 ggagagagca aagtagccat agtaatgggg gtacttgtct ttagggggaa tgtgcctccg 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 atcagtgacc aaacaggaaa aaaccgccct taacatggcc cgctttatca gaagccagac 1740

1681 tagtcactgg tttgtccttt tttggcggga attgtaccgg gcgaaatagt cttcggtctg 1740

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

1741 attaacgctt ctggagaaac tcaacgagct ggacgcggat gaacaggcag acatctgtga 1800

1741 taattgcgaa gacctctttg agttgctcga cctgcgccta cttgtccgtc tgtagacact 1800

.......... .......... ROP .......... .......... ..........

.......... .......... ......... PvuII(1836) ......... ..........

1801 atcgcttcac gaccacgctg atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga 1860

1801 tagcgaagtg ctggtgcgac tactcgaaat ggcgtcgacg gagcgcgcaa agccactact 1860

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 1920

1861 gccacttttg gagactgtgt acgtcgaggg cctctgccag tgtcgaacag acattcgcct 1920

ROP .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc 1980

1921 acggccctcg tctgttcggg cagtcccgcg cagtcgccca caaccgccca cagccccgcg 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca 2040

1981 tcggtactgg gtcagtgcat cgctatcgcc tcacatatga ccgaattgat acgccgtagt 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gagcagattg tactgagagt gcaccatata tgcggtgtga aataccgcac agatgcgtaa 2100

2041 ctcgtctaac atgactctca cgtggtatat acgccacact ttatggcgtg tctacgcatt 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ggagaaaata ccgcatcagg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 2160

2101 cctcttttat ggcgtagtcc gcgagaaggc gaaggagcga gtgactgagc gacgcgagcc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 2220

2161 agcaagccga cgccgctcgc catagtcgag tgagtttccg ccattatgcc aataggtgtc 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 2280

2221 ttagtcccct attgcgtcct ttcttgtaca ctcgttttcc ggtcgttttc cggtccttgg 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 2340

2281 catttttccg gcgcaacgac cgcaaaaagg tatccgaggc ggggggactg ctcgtagtgt 2340

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2341 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 2400

2341 ttttagctgc gagttcagtc tccaccgctt tgggctgtcc tgatatttct atggtccgca 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 2460

2401 aagggggacc ttcgagggag cacgcgagag gacaaggctg ggacggcgaa tggcctatgg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 2520

2461 acaggcggaa agagggaagc ccttcgcacc gcgaaagagt atcgagtgcg acatccatag 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 2580

2521 agtcaagcca catccagcaa gcgaggttcg acccgacaca cgtgcttggg gggcaagtcg 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 2640

2581 ggctggcgac gcggaatagg ccattgatag cagaactcag gttgggccat tctgtgctga 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 2700

2641 atagcggtga ccgtcgtcgg tgaccattgt cctaatcgtc tcgctccata catccgccac 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 2760

2701 gatgtctcaa gaacttcacc accggattga tgccgatgtg atcttcctgt cataaaccat 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 2820

2761 agacgcgaga cgacttcggt caatggaagc ctttttctca accatcgaga actaggccgt 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 2880

2821 ttgtttggtg gcgaccatcg ccaccaaaaa aacaaacgtt cgtcgtctaa tgcgcgtctt 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 2940

2881 tttttcctag agttcttcta ggaaactaga aaagatgccc cagactgcga gtcaccttgc 2940

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 3000

2941 ttttgagtgc aattccctaa aaccagtact ctaatagttt ttcctagaag tggatctagg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 3060

3001 aaaatttaat ttttacttca aaatttagtt agatttcata tatactcatt tgaaccagac 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 3120

3061 tgtcaatggt tacgaattag tcactccgtg gatagagtcg ctagacagat aaagcaagta 3120

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3121 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 3180

3121 ggtatcaacg gactgagggg cagcacatct attgatgcta tgccctcccg aatggtagac 3180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 3240

3181 cggggtcacg acgttactat ggcgctctgg gtgcgagtgg ccgaggtcta aatagtcgtt 3240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 3300

3241 atttggtcgg tcggccttcc cggctcgcgt cttcaccagg acgttgaaat aggcggaggt 3300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 3360

3301 aggtcagata attaacaacg gcccttcgat ctcattcatc aagcggtcaa ttatcaaacg 3360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... PstI(3383) .......... .......... ..........

3361 gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 3420

3361 cgttgcaaca acggtaacga cgtccgtagc accacagtgc gagcagcaaa ccataccgaa 3420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 3480

3421 gtaagtcgag gccaagggtt gctagttccg ctcaatgtac tagggggtac aacacgtttt 3480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 3540

3481 ttcgccaatc gaggaagcca ggaggctagc aacagtcttc attcaaccgg cgtcacaata 3540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 3600

3541 gtgagtacca ataccgtcgt gacgtattaa gagaatgaca gtacggtagg cattctacga 3600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 3660

3601 aaagacactg accactcatg agttggttca gtaagactct tatcacatac gccgctggct 3660

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag 3720

3661 caacgagaac gggccgcagt tgtgccctat tatggcgcgg tgtatcgtct tgaaattttc 3720

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 3780

3721 acgagtagta accttttgca agaagccccg cttttgagag ttcctagaat ggcgacaact 3780

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 3840

3781 ctaggtcaag ctacattggg tgagcacgtg ggttgactag aagtcgtaga aaatgaaagt 3840

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 3900

3841 ggtcgcaaag acccactcgt ttttgtcctt ccgttttacg gcgttttttc ccttattccc 3900

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 3960

3901 gctgtgcctt tacaacttat gagtatgaga aggaaaaagt tataataact tcgtaaatag 3960

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 4020

3961 tcccaataac agagtactcg cctatgtata aacttacata aatcttttta tttgtttatc 4020

.......... .......... AmpR_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca 4080

4021 cccaaggcgc gtgtaaaggg gcttttcacg gtggactgca gattctttgg taataatagt 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... ......... EcoRI(4131)

4081 tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaa....... 4133

4081 actgtaattg gatattttta tccgcatagt gctccgggaa agcagaagtt ctt....... 4133

.......... .......... .......... .......... .......... ..........