}WI}WILOCUS pET5c 4132 bp DNA SYN 26-Aug-2007 DEFINITION pET5c ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4132 /organism="pET5c" /mol_type="other DNA" misc_feature complement(11..43) /label="T7_gene10_leader" misc_feature complement(11..43) /label="T7_leader" misc_feature complement(26..44) /label="Xpress_fwd_primer" misc_feature complement(52..68) /label="T7_transl_en_RBS" promoter complement(105..123) /label="T7_promoter" misc_feature complement(207..226) /label="pBRrevBam_primer" gene complement(155..1045) /label="tet(1191-300)" /gene="tet(1191-300)" CDS 260..1045 /label="ORF frame 2" /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF LMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFG EDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLA FATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPL IVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*" misc_feature 1684..1875 /label="ROP" misc_feature complement(1869..1891) /label="pGEX_3_primer" rep_origin complement(2290..2909) /label="pBR322_origin" CDS complement(3064..3924) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(3064..3924) /label="Ampicillin" /gene="Ampicillin" promoter complement(3966..3994) /label="AmpR_promoter" ORIGIN 1 TTCGGATCCG ACCCATTTGC TGTCCACCAG TCATGCTAGC CATATGTATA TCTCCTTCTT 61 AAAGTTAAAC AAAATTATTT CTAGAGGGAA ACCGTTGTGG TCTCCCTATA GTGAGTCGTA 121 TTAATTTCGC GGGATCGAGA TCTCGATCCT CTACGCCGGA CGCATCGTGG CCGGCATCAC 181 CGGCGCCACA GGTGCGGTTG CTGGCGCCTA TATCGCCGAC ATCACCGATG GGGAAGATCG 241 GGCTCGCCAC TTCGGGCTCA TGAGCGCTTG TTTCGGCGTG GGTATGGTGG CAGGCCCCGT 301 GGCCGGGGGA CTGTTGGGCG CCATCTCCTT GCATGCACCA TTCCTTGCGG CGGCGGTGCT 361 CAACGGCCTC AACCTACTAC TGGGCTGCTT CCTAATGCAG GAGTCGCATA AGGGAGAGCG 421 TCGACCGATG CCCTTGAGAG CCTTCAACCC AGTCAGCTCC TTCCGGTGGG CGCGGGGCAT 481 GACTATCGTC GCCGCACTTA TGACTGTCTT CTTTATCATG CAACTCGTAG GACAGGTGCC 541 GGCAGCGCTC TGGGTCATTT TCGGCGAGGA CCGCTTTCGC TGGAGCGCGA CGATGATCGG 601 CCTGTCGCTT GCGGTATTCG GAATCTTGCA CGCCCTCGCT CAAGCCTTCG TCACTGGTCC 661 CGCCACCAAA CGTTTCGGCG AGAAGCAGGC CATTATCGCC GGCATGGCGG CCGACGCGCT 721 GGGCTACGTC TTGCTGGCGT TCGCGACGCG AGGCTGGATG GCCTTCCCCA TTATGATTCT 781 TCTCGCTTCC GGCGGCATCG GGATGCCCGC GTTGCAGGCC ATGCTGTCCA GGCAGGTAGA 841 TGACGACCAT CAGGGACAGC TTCAAGGATC GCTCGCGGCT CTTACCAGCC TAACTTCGAT 901 CACTGGACCG CTGATCGTCA CGGCGATTTA TGCCGCCTCG GCGAGCACAT GGAACGGGTT 961 GGCATGGATT GTAGGCGCCG CCCTATACCT TGTCTGCCTC CCCGCGTTGC GTCGCGGTGC 1021 ATGGAGCCGG GCCACCTCGA CCTGAATGGA AGCCGGCGGC ACCTCGCTAA CGGATTCACC 1081 ACTCCAAGAA TTGGAGCCAA TCAATTCTTG CGGAGAACTG TGAATGCGCA AACCAACCCT 1141 TGGCAGAACA TATCCATCGC GTCCGCCATC TCCAGCAGCC GCACGCGGCG CATCTCGGGC 1201 AGCGTTGGGT CCTGGCCACG GGTGCGCATG ATCGTGCTCC TGTCGTTGAG GACCCGGCTA 1261 GGCTGGCGGG GTTGCCTTAC TGGTTAGCAG AATGAATCAC CGATACGCGA GCGAACGTGA 1321 AGCGACTGCT GCTGCAAAAC GTCTGCGACC TGAGCAACAA CATGAATGGT CTTCGGTTTC 1381 CGTGTTTCGT AAAGTCTGGA AACGCGGAAG TCAGCGCCCT GCACCATTAT GTTCCGGATC 1441 TGCATCGCAG GATGCTGCTG GCTACCCTGT GGAACACCTA CATCTGTATT AACGAAGCGC 1501 TGGCATTGAC CCTGAGTGAT TTTTCTCTGG TCCCGCCGCA TCCATACCGC CAGTTGTTTA 1561 CCCTCACAAC GTTCCAGTAA CCGGGCATGT TCATCATCAG TAACCCGTAT CGTGAGCATC 1621 CTCTCTCGTT TCATCGGTAT CATTACCCCC ATGAACAGAA ATCCCCCTTA CACGGAGGCA 1681 TCAGTGACCA AACAGGAAAA AACCGCCCTT AACATGGCCC GCTTTATCAG AAGCCAGACA 1741 TTAACGCTTC TGGAGAAACT CAACGAGCTG GACGCGGATG AACAGGCAGA CATCTGTGAA 1801 TCGCTTCACG ACCACGCTGA TGAGCTTTAC CGCAGCTGCC TCGCGCGTTT CGGTGATGAC 1861 GGTGAAAACC TCTGACACAT GCAGCTCCCG GAGACGGTCA CAGCTTGTCT GTAAGCGGAT 1921 GCCGGGAGCA GACAAGCCCG TCAGGGCGCG TCAGCGGGTG TTGGCGGGTG TCGGGGCGCA 1981 GCCATGACCC AGTCACGTAG CGATAGCGGA GTGTATACTG GCTTAACTAT GCGGCATCAG 2041 AGCAGATTGT ACTGAGAGTG CACCATATAT GCGGTGTGAA ATACCGCACA GATGCGTAAG 2101 GAGAAAATAC CGCATCAGGC GCTCTTCCGC TTCCTCGCTC ACTGACTCGC TGCGCTCGGT 2161 CGTTCGGCTG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 2221 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 2281 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 2341 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 2401 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 2461 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 2521 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 2581 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 2641 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 2701 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGGACAG TATTTGGTAT 2761 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 2821 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 2881 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 2941 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 3001 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAA CTTGGTCTGA 3061 CAGTTACCAA TGCTTAATCA GTGAGGCACC TATCTCAGCG ATCTGTCTAT TTCGTTCATC 3121 CATAGTTGCC TGACTCCCCG TCGTGTAGAT AACTACGATA CGGGAGGGCT TACCATCTGG 3181 CCCCAGTGCT GCAATGATAC CGCGAGACCC ACGCTCACCG GCTCCAGATT TATCAGCAAT 3241 AAACCAGCCA GCCGGAAGGG CCGAGCGCAG AAGTGGTCCT GCAACTTTAT CCGCCTCCAT 3301 CCAGTCTATT AATTGTTGCC GGGAAGCTAG AGTAAGTAGT TCGCCAGTTA ATAGTTTGCG 3361 CAACGTTGTT GCCATTGCTG CAGGCATCGT GGTGTCACGC TCGTCGTTTG GTATGGCTTC 3421 ATTCAGCTCC GGTTCCCAAC GATCAAGGCG AGTTACATGA TCCCCCATGT TGTGCAAAAA 3481 AGCGGTTAGC TCCTTCGGTC CTCCGATCGT TGTCAGAAGT AAGTTGGCCG CAGTGTTATC 3541 ACTCATGGTT ATGGCAGCAC TGCATAATTC TCTTACTGTC ATGCCATCCG TAAGATGCTT 3601 TTCTGTGACT GGTGAGTACT CAACCAAGTC ATTCTGAGAA TAGTGTATGC GGCGACCGAG 3661 TTGCTCTTGC CCGGCGTCAA CACGGGATAA TACCGCGCCA CATAGCAGAA CTTTAAAAGT 3721 GCTCATCATT GGAAAACGTT CTTCGGGGCG AAAACTCTCA AGGATCTTAC CGCTGTTGAG 3781 ATCCAGTTCG ATGTAACCCA CTCGTGCACC CAACTGATCT TCAGCATCTT TTACTTTCAC 3841 CAGCGTTTCT GGGTGAGCAA AAACAGGAAG GCAAAATGCC GCAAAAAAGG GAATAAGGGC 3901 GACACGGAAA TGTTGAATAC TCATACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA 3961 GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG 4021 GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGTC TAAGAAACCA TTATTATCAT 4081 GACATTAACC TATAAAAATA GGCGTATCAC GAGGCCCTTT CGTCTTCAAG AA //$pET5c "Times New Roman}WIC$ +T7_gene10_leader"Times New Roman}WIC$ + T7_leader"Times New Roman}WICɆ$,Xpress_fwd_pri"Times New Roman*}WI$4DT7_transl_en_RBS"Times New Roman}WIC$i{T7_pro"Times New Roman}WICɆ$ tet(1191-300)"Times New Roman}WIC$ pBRrevBam_pri"Times New Roman}WI$ORF 2"Times New Roman}WIC$SROP"Times New Roman}WIC$Mc pGEX_3_pri"Times New Roman}WICɆ$]  pBR322_origin"Times New Roman}WI$ TORF 1"Times New Roman}WIC$ T Ampicillin"Times New Roman(}WIC$~AmpR_pro"Times New Roman }WI.BamHI(4),NheI(35),NdeI(42),XbaI(80),BglII(138)"Times New Roman$q}qiqULU }WISalI"Times New Roman$xB }WIEagI(708),NruI(743)"Times New Roman$:K\\ }WIMscI"Times New Roman$Obuu }WIPvuII"Times New Roman+$9L^^m }WIPstI"Times New Roman6 $y>y }WIAatII(4059),EcoRI(4130)"Times New Roman$T~Rj;[=;[ }WItest"Times New Roman$u}vv:C