Sequence of pET5c

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Sequence of pET5c

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Sequence of pET5c : 4132 BP; 948 A; 1131 C; 1072 G; 981 T; 0 other;

BamHI(4) .......... .......... NheI(35) .......... ..........

1 ttcggatccg acccatttgc tgtccaccag tcatgctagc catatgtata tctccttctt 60

1 aagcctaggc tgggtaaacg acaggtggtc agtacgatcg gtatacatat agaggaagaa 60

....... T7_gene10_leader T7_leader Xpress_fwd_pri T7_transl_en_RBS

.......... .......... .......... .......... .......... ..........

61 aaagttaaac aaaattattt ctagagggaa accgttgtgg tctccctata gtgagtcgta 120

61 tttcaatttg ttttaataaa gatctccctt tggcaacacc agagggatat cactcagcat 120

.......... .......... .......... .......... .......... T7_pro

.......... BglII(138) .......... .......... .......... ..........

121 ttaatttcgc gggatcgaga tctcgatcct ctacgccgga cgcatcgtgg ccggcatcac 180

121 aattaaagcg ccctagctct agagctagga gatgcggcct gcgtagcacc ggccgtagtg 180

.......... .......... .......... ........ tet(1191-300) ........

.......... .......... .......... .......... .......... ..........

181 cggcgccaca ggtgcggttg ctggcgccta tatcgccgac atcaccgatg gggaagatcg 240

181 gccgcggtgt ccacgccaac gaccgcggat atagcggctg tagtggctac cccttctagc 240

.......... ...... tet(1191-300) pBRrevBam_pri ...... ..........

.......... .......... .......... .......... .......... ..........

241 ggctcgccac ttcgggctca tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt 300

241 ccgagcggtg aagcccgagt actcgcgaac aaagccgcac ccataccacc gtccggggca 300

.......... ........ tet(1191-300) ORF 2 ........ ..........

.......... .......... .......... .......... .......... ..........

301 ggccggggga ctgttgggcg ccatctcctt gcatgcacca ttccttgcgg cggcggtgct 360

301 ccggccccct gacaacccgc ggtagaggaa cgtacgtggt aaggaacgcc gccgccacga 360

.......... ..... tet(1191-300) ORF 2 ..... .......... ..........

.......... .......... .......... .......... .......... SalI(420)

361 caacggcctc aacctactac tgggctgctt cctaatgcag gagtcgcata agggagagcg 420

361 gttgccggag ttggatgatg acccgacgaa ggattacgtc ctcagcgtat tccctctcgc 420

.......... ..... tet(1191-300) ORF 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tcgaccgatg cccttgagag ccttcaaccc agtcagctcc ttccggtggg cgcggggcat 480

421 agctggctac gggaactctc ggaagttggg tcagtcgagg aaggccaccc gcgccccgta 480

.......... ..... tet(1191-300) ORF 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gactatcgtc gccgcactta tgactgtctt ctttatcatg caactcgtag gacaggtgcc 540

481 ctgatagcag cggcgtgaat actgacagaa gaaatagtac gttgagcatc ctgtccacgg 540

.......... ..... tet(1191-300) ORF 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ggcagcgctc tgggtcattt tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg 600

541 ccgtcgcgag acccagtaaa agccgctcct ggcgaaagcg acctcgcgct gctactagcc 600

.......... ..... tet(1191-300) ORF 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cctgtcgctt gcggtattcg gaatcttgca cgccctcgct caagccttcg tcactggtcc 660

601 ggacagcgaa cgccataagc cttagaacgt gcgggagcga gttcggaagc agtgaccagg 660

.......... ..... tet(1191-300) ORF 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

661 cgccaccaaa cgtttcggcg agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct 720

661 gcggtggttt gcaaagccgc tcttcgtccg gtaatagcgg ccgtaccgcc ggctgcgcga 720

.......... ..... tet(1191-300) ORF 2 ..... .......... ..........

.......... .......... NruI(743) .......... .......... ..........

721 gggctacgtc ttgctggcgt tcgcgacgcg aggctggatg gccttcccca ttatgattct 780

721 cccgatgcag aacgaccgca agcgctgcgc tccgacctac cggaaggggt aatactaaga 780

.......... ..... tet(1191-300) ORF 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tctcgcttcc ggcggcatcg ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga 840

781 agagcgaagg ccgccgtagc cctacgggcg caacgtccgg tacgacaggt ccgtccatct 840

.......... ..... tet(1191-300) ORF 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tgacgaccat cagggacagc ttcaaggatc gctcgcggct cttaccagcc taacttcgat 900

841 actgctggta gtccctgtcg aagttcctag cgagcgccga gaatggtcgg attgaagcta 900

.......... ..... tet(1191-300) ORF 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

901 cactggaccg ctgatcgtca cggcgattta tgccgcctcg gcgagcacat ggaacgggtt 960

901 gtgacctggc gactagcagt gccgctaaat acggcggagc cgctcgtgta ccttgcccaa 960

.......... ..... tet(1191-300) ORF 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggcatggatt gtaggcgccg ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc 1020

961 ccgtacctaa catccgcggc gggatatgga acagacggag gggcgcaacg cagcgccacg 1020

.......... ..... tet(1191-300) ORF 2 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 atggagccgg gccacctcga cctgaatgga agccggcggc acctcgctaa cggattcacc 1080

1021 tacctcggcc cggtggagct ggacttacct tcggccgccg tggagcgatt gcctaagtgg 1080

..... tet(1191-300) ORF 2 ..... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 actccaagaa ttggagccaa tcaattcttg cggagaactg tgaatgcgca aaccaaccct 1140

1081 tgaggttctt aacctcggtt agttaagaac gcctcttgac acttacgcgt ttggttggga 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tggcagaaca tatccatcgc gtccgccatc tccagcagcc gcacgcggcg catctcgggc 1200

1141 accgtcttgt ataggtagcg caggcggtag aggtcgtcgg cgtgcgccgc gtagagcccg 1200

.......... .......... .......... .......... .......... ..........

.......... MscI(1215) .......... .......... .......... ..........

1201 agcgttgggt cctggccacg ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta 1260

1201 tcgcaaccca ggaccggtgc ccacgcgtac tagcacgagg acagcaactc ctgggccgat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ggctggcggg gttgccttac tggttagcag aatgaatcac cgatacgcga gcgaacgtga 1320

1261 ccgaccgccc caacggaatg accaatcgtc ttacttagtg gctatgcgct cgcttgcact 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 agcgactgct gctgcaaaac gtctgcgacc tgagcaacaa catgaatggt cttcggtttc 1380

1321 tcgctgacga cgacgttttg cagacgctgg actcgttgtt gtacttacca gaagccaaag 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 cgtgtttcgt aaagtctgga aacgcggaag tcagcgccct gcaccattat gttccggatc 1440

1381 gcacaaagca tttcagacct ttgcgccttc agtcgcggga cgtggtaata caaggcctag 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tgcatcgcag gatgctgctg gctaccctgt ggaacaccta catctgtatt aacgaagcgc 1500

1441 acgtagcgtc ctacgacgac cgatgggaca ccttgtggat gtagacataa ttgcttcgcg 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tggcattgac cctgagtgat ttttctctgg tcccgccgca tccataccgc cagttgttta 1560

1501 accgtaactg ggactcacta aaaagagacc agggcggcgt aggtatggcg gtcaacaaat 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ccctcacaac gttccagtaa ccgggcatgt tcatcatcag taacccgtat cgtgagcatc 1620

1561 gggagtgttg caaggtcatt ggcccgtaca agtagtagtc attgggcata gcactcgtag 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ctctctcgtt tcatcggtat cattaccccc atgaacagaa atccccctta cacggaggca 1680

1621 gagagagcaa agtagccata gtaatggggg tacttgtctt tagggggaat gtgcctccgt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcagtgacca aacaggaaaa aaccgccctt aacatggccc gctttatcag aagccagaca 1740

1681 agtcactggt ttgtcctttt ttggcgggaa ttgtaccggg cgaaatagtc ttcggtctgt 1740

.......... .......... .......... ROP .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ttaacgcttc tggagaaact caacgagctg gacgcggatg aacaggcaga catctgtgaa 1800

1741 aattgcgaag acctctttga gttgctcgac ctgcgcctac ttgtccgtct gtagacactt 1800

.......... .......... ROP .......... .......... ..........

.......... .......... ......... PvuII(1835) ......... ..........

1801 tcgcttcacg accacgctga tgagctttac cgcagctgcc tcgcgcgttt cggtgatgac 1860

1801 agcgaagtgc tggtgcgact actcgaaatg gcgtcgacgg agcgcgcaaa gccactactg 1860

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ggtgaaaacc tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat 1920

1861 ccacttttgg agactgtgta cgtcgagggc ctctgccagt gtcgaacaga cattcgccta 1920

ROP pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca 1980

1921 cggccctcgt ctgttcgggc agtcccgcgc agtcgcccac aaccgcccac agccccgcgt 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gccatgaccc agtcacgtag cgatagcgga gtgtatactg gcttaactat gcggcatcag 2040

1981 cggtactggg tcagtgcatc gctatcgcct cacatatgac cgaattgata cgccgtagtc 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 agcagattgt actgagagtg caccatatat gcggtgtgaa ataccgcaca gatgcgtaag 2100

2041 tcgtctaaca tgactctcac gtggtatata cgccacactt tatggcgtgt ctacgcattc 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gagaaaatac cgcatcaggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 2160

2101 ctcttttatg gcgtagtccg cgagaaggcg aaggagcgag tgactgagcg acgcgagcca 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 2220

2161 gcaagccgac gccgctcgcc atagtcgagt gagtttccgc cattatgcca ataggtgtct 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 2280

2221 tagtccccta ttgcgtcctt tcttgtacac tcgttttccg gtcgttttcc ggtccttggc 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 2340

2281 atttttccgg cgcaacgacc gcaaaaaggt atccgaggcg gggggactgc tcgtagtgtt 2340

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

2341 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 2400

2341 tttagctgcg agttcagtct ccaccgcttt gggctgtcct gatatttcta tggtccgcaa 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 2460

2401 agggggacct tcgagggagc acgcgagagg acaaggctgg gacggcgaat ggcctatgga 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 2520

2461 caggcggaaa gagggaagcc cttcgcaccg cgaaagagta tcgagtgcga catccataga 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 2580

2521 gtcaagccac atccagcaag cgaggttcga cccgacacac gtgcttgggg ggcaagtcgg 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 2640

2581 gctggcgacg cggaataggc cattgatagc agaactcagg ttgggccatt ctgtgctgaa 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 2700

2641 tagcggtgac cgtcgtcggt gaccattgtc ctaatcgtct cgctccatac atccgccacg 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat 2760

2701 atgtctcaag aacttcacca ccggattgat gccgatgtga tcttcctgtc ataaaccata 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 2820

2761 gacgcgagac gacttcggtc aatggaagcc tttttctcaa ccatcgagaa ctaggccgtt 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 2880

2821 tgtttggtgg cgaccatcgc caccaaaaaa acaaacgttc gtcgtctaat gcgcgtcttt 2880

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 2940

2881 ttttcctaga gttcttctag gaaactagaa aagatgcccc agactgcgag tcaccttgct 2940

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 3000

2941 tttgagtgca attccctaaa accagtactc taatagtttt tcctagaagt ggatctagga 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 3060

3001 aaatttaatt tttacttcaa aatttagtta gatttcatat atactcattt gaaccagact 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 3120

3061 gtcaatggtt acgaattagt cactccgtgg atagagtcgc tagacagata aagcaagtag 3120

.......... .......... ....... ORF 1 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

3121 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 3180

3121 gtatcaacgg actgaggggc agcacatcta ttgatgctat gccctcccga atggtagacc 3180

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 3240

3181 ggggtcacga cgttactatg gcgctctggg tgcgagtggc cgaggtctaa atagtcgtta 3240

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 3300

3241 tttggtcggt cggccttccc ggctcgcgtc ttcaccagga cgttgaaata ggcggaggta 3300

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 3360

3301 ggtcagataa ttaacaacgg cccttcgatc tcattcatca agcggtcaat tatcaaacgc 3360

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... PstI(3382) .......... .......... ..........

3361 caacgttgtt gccattgctg caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 3420

3361 gttgcaacaa cggtaacgac gtccgtagca ccacagtgcg agcagcaaac cataccgaag 3420

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 3480

3421 taagtcgagg ccaagggttg ctagttccgc tcaatgtact agggggtaca acacgttttt 3480

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 3540

3481 tcgccaatcg aggaagccag gaggctagca acagtcttca ttcaaccggc gtcacaatag 3540

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 3600

3541 tgagtaccaa taccgtcgtg acgtattaag agaatgacag tacggtaggc attctacgaa 3600

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 3660

3601 aagacactga ccactcatga gttggttcag taagactctt atcacatacg ccgctggctc 3660

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ttgctcttgc ccggcgtcaa cacgggataa taccgcgcca catagcagaa ctttaaaagt 3720

3661 aacgagaacg ggccgcagtt gtgccctatt atggcgcggt gtatcgtctt gaaattttca 3720

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 3780

3721 cgagtagtaa ccttttgcaa gaagccccgc ttttgagagt tcctagaatg gcgacaactc 3780

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 3840

3781 taggtcaagc tacattgggt gagcacgtgg gttgactaga agtcgtagaa aatgaaagtg 3840

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 3900

3841 gtcgcaaaga cccactcgtt tttgtccttc cgttttacgg cgttttttcc cttattcccg 3900

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 3960

3901 ctgtgccttt acaacttatg agtatgagaa ggaaaaagtt ataataactt cgtaaatagt 3960

....... ORF 1 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 4020

3961 cccaataaca gagtactcgc ctatgtataa acttacataa atctttttat ttgtttatcc 4020

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat 4080

4021 ccaaggcgcg tgtaaagggg cttttcacgg tggactgcag attctttggt aataatagta 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... EcoRI(4130) .........

4081 gacattaacc tataaaaata ggcgtatcac gaggcccttt cgtcttcaag aa........ 4132

4081 ctgtaattgg atatttttat ccgcatagtg ctccgggaaa gcagaagttc tt........ 4132

.......... .......... .......... .......... .......... ..........