Sequence of pET_11

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Sequence of pET_11_a

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Sequence of pET 11 a : 5676 BP; 1299 A; 1550 C; 1495 G; 1332 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 240

181 cgccctatag gcctatatca aggaggaaag tcgttttttg gggagttctg ggcaaatctc 240

.......... .......... .......... T7_term .......... ..........

.......... .......... .......... .......... .......... ..........

241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 300

241 cggggttccc caatacgatc aataacgagt cgccaccgtc gtcggttgag tcgaaggaaa 300

.......... ... T7_term T7_Terminal_pri ... .......... ..........

.......... BamHI(319) .......... .......... .......... NheI(352)

301 cgggctttgt tagcagccgg atcccgaccc atttgctgtc caccagtcat gctagccata 360

301 gcccgaaaca atcgtcggcc tagggctggg taaacgacag gtggtcagta cgatcggtat 360

T7_term .......... .......... T7_gene10_leader T7_leader Xpress_fwd_pri

.......... .......... .......... .......... .......... ..........

361 tgtatatctc cttcttaaag ttaaacaaaa ttatttctag aggggaattg ttatccgctc 420

361 acatatagag gaagaatttc aatttgtttt aataaagatc tccccttaac aataggcgag 420

....... T7_transl_en_RBS ....... .......... .......... lacO

.......... .......... .......... .......... BglII(463) ..........

421 acaattcccc tatagtgagt cgtattaatt tcgcgggatc gagatctcga tcctctacgc 480

421 tgttaagggg atatcactca gcataattaa agcgccctag ctctagagct aggagatgcg 480

lacO T7_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc 540

481 gcctgcgtag caccggccgt agtggccgcg gtgtccacgc caacgaccgc ggatatagcg 540

.......... .......... .......... .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

541 cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg 600

541 gctgtagtgg ctaccccttc tagcccgagc ggtgaagccc gagtactcgc gaacaaagcc 600

pBRrevBam_pri ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc 660

601 gcacccatac caccgtccgg ggcaccggcc ccctgacaac ccgcggtaga ggaacgtacg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat 720

661 tggtaaggaa cgccgccgcc acgagttgcc ggagttggat gatgacccga cgaaggatta 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcaggagtcg cataagggag agcgtcgaga tcccggacac catcgaatgg cgcaaaacct 780

721 cgtcctcagc gtattccctc tcgcagctct agggcctgtg gtagcttacc gcgttttgga 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttcgcggtat ggcatgatag cgcccggaag agagtcaatt cagggtggtg aatgtgaaac 840

781 aagcgccata ccgtactatc gcgggccttc tctcagttaa gtcccaccac ttacactttg 840

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

841 cagtaacgtt atacgatgtc gcagagtatg ccggtgtctc ttatcagacc gtttcccgcg 900

841 gtcattgcaa tatgctacag cgtctcatac ggccacagag aatagtctgg caaagggcgc 900

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tggtgaacca ggccagccac gtttctgcga aaacgcggga aaaagtggaa gcggcgatgg 960

901 accacttggt ccggtcggtg caaagacgct tttgcgccct ttttcacctt cgccgctacc 960

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 cggagctgaa ttacattccc aaccgcgtgg cacaacaact ggcgggcaaa cagtcgttgc 1020

961 gcctcgactt aatgtaaggg ttggcgcacc gtgttgttga ccgcccgttt gtcagcaacg 1020

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tgattggcgt tgccacctcc agtctggccc tgcacgcgcc gtcgcaaatt gtcgcggcga 1080

1021 actaaccgca acggtggagg tcagaccggg acgtgcgcgg cagcgtttaa cagcgccgct 1080

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttaaatctcg cgccgatcaa ctgggtgcca gcgtggtggt gtcgatggta gaacgaagcg 1140

1081 aatttagagc gcggctagtt gacccacggt cgcaccacca cagctaccat cttgcttcgc 1140

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1199)

1141 gcgtcgaagc ctgtaaagcg gcggtgcaca atcttctcgc gcaacgcgtc agtgggctga 1200

1141 cgcagcttcg gacatttcgc cgccacgtgt tagaagagcg cgttgcgcag tcacccgact 1200

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tcattaacta tccgctggat gaccaggatg ccattgctgt ggaagctgcc tgcactaatg 1260

1201 agtaattgat aggcgaccta ctggtcctac ggtaacgaca ccttcgacgg acgtgattac 1260

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttccggcgtt atttcttgat gtctctgacc agacacccat caacagtatt attttctccc 1320

1261 aaggccgcaa taaagaacta cagagactgg tctgtgggta gttgtcataa taaaagaggg 1320

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 atgaagacgg tacgcgactg ggcgtggagc atctggtcgc attgggtcac cagcaaatcg 1380

1321 tacttctgcc atgcgctgac ccgcacctcg tagaccagcg taacccagtg gtcgtttagc 1380

.......... .......... lacI ORF 1 .......... .......... ..........

.......... ApaI(1396) .......... .......... .......... ..........

1381 cgctgttagc gggcccatta agttctgtct cggcgcgtct gcgtctggct ggctggcata 1440

1381 gcgacaatcg cccgggtaat tcaagacaga gccgcgcaga cgcagaccga ccgaccgtat 1440

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aatatctcac tcgcaatcaa attcagccga tagcggaacg ggaaggcgac tggagtgcca 1500

1441 ttatagagtg agcgttagtt taagtcggct atcgccttgc ccttccgctg acctcacggt 1500

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 tgtccggttt tcaacaaacc atgcaaatgc tgaatgaggg catcgttccc actgcgatgc 1560

1501 acaggccaaa agttgtttgg tacgtttacg acttactccc gtagcaaggg tgacgctacg 1560

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tggttgccaa cgatcagatg gcgctgggcg caatgcgcgc cattaccgag tccgggctgc 1620

1561 accaacggtt gctagtctac cgcgacccgc gttacgcgcg gtaatggctc aggcccgacg 1620

.......... .......... lacI ORF 1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcgttggtgc ggatatctcg gtagtgggat acgacgatac cgaagacagc tcatgttata 1680

1621 cgcaaccacg cctatagagc catcacccta tgctgctatg gcttctgtcg agtacaatat 1680

.......... .......... lacI ORF 1 .......... .......... ..........

.......... HpaI(1691) .......... .......... .......... ..........

1681 tcccgccgtt aaccaccatc aaacaggatt ttcgcctgct ggggcaaacc agcgtggacc 1740

1681 agggcggcaa ttggtggtag tttgtcctaa aagcggacga ccccgtttgg tcgcacctgg 1740

.......... .......... lacI ORF 1 ORF 2 .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcttgctgca actctctcag ggccaggcgg tgaagggcaa tcagctgttg cccgtctcac 1800

1741 cgaacgacgt tgagagagtc ccggtccgcc acttcccgtt agtcgacaac gggcagagtg 1800

.......... ....... lacI ORF 1 ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tggtgaaaag aaaaaccacc ctggcgccca atacgcaaac cgcctctccc cgcgcgttgg 1860

1801 accacttttc tttttggtgg gaccgcgggt tatgcgtttg gcggagaggg gcgcgcaacc 1860

.......... ....... lacI ORF 1 ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc 1920

1861 ggctaagtaa ttacgtcgac cgtgctgtcc aaagggctga cctttcgccc gtcactcgcg 1920

.......... ....... lacI ORF 1 ORF 2 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aacgcaatta atgtaagtta gctcactcat taggcaccgg gatctcgacc gatgcccttg 1980

1921 ttgcgttaat tacattcaat cgagtgagta atccgtggcc ctagagctgg ctacgggaac 1980

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca 2040

1981 tctcggaagt tgggtcagtc gaggaaggcc acccgcgccc cgtactgata gcagcggcgt 2040

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctctgggtc 2100

2041 gaatactgac agaagaaata gtacgttgag catcctgtcc acggccgtcg cgagacccag 2100

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2101 attttcggcg aggaccgctt tcgctggagc gcgacgatga tcggcctgtc gcttgcggta 2160

2101 taaaagccgc tcctggcgaa agcgacctcg cgctgctact agccggacag cgaacgccat 2160

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ttcggaatct tgcacgccct cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc 2220

2161 aagccttaga acgtgcggga gcgagttcgg aagcagtgac cagggcggtg gtttgcaaag 2220

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ggcgagaagc aggccattat cgccggcatg gcggccgacg cgctgggcta cgtcttgctg 2280

2221 ccgctcttcg tccggtaata gcggccgtac cgccggctgc gcgacccgat gcagaacgac 2280

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

NruI(2288) .......... .......... .......... .......... ..........

2281 gcgttcgcga cgcgaggctg gatggccttc cccattatga ttcttctcgc ttccggcggc 2340

2281 cgcaagcgct gcgctccgac ctaccggaag gggtaatact aagaagagcg aaggccgccg 2340

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2341 atcgggatgc ccgcgttgca ggccatgctg tccaggcagg tagatgacga ccatcaggga 2400

2341 tagccctacg ggcgcaacgt ccggtacgac aggtccgtcc atctactgct ggtagtccct 2400

.......... .. ORF 2 ORF 2 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2401 cagcttcaag gatcgctcgc ggctcttacc agcctaactt cgatcactgg accgctgatc 2460

2401 gtcgaagttc ctagcgagcg ccgagaatgg tcggattgaa gctagtgacc tggcgactag 2460

.......... ..... ORF 2 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtcacggcga tttatgccgc ctcggcgagc acatggaacg ggttggcatg gattgtaggc 2520

2461 cagtgccgct aaatacggcg gagccgctcg tgtaccttgc ccaaccgtac ctaacatccg 2520

.......... ..... ORF 2 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gccgccctat accttgtctg cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc 2580

2521 cggcgggata tggaacagac ggaggggcgc aacgcagcgc cacgtacctc ggcccggtgg 2580

.......... ..... ORF 2 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcgacctgaa tggaagccgg cggcacctcg ctaacggatt caccactcca agaattggag 2640

2581 agctggactt accttcggcc gccgtggagc gattgcctaa gtggtgaggt tcttaacctc 2640

ORF 2 tet(1191-576) .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ccaatcaatt cttgcggaga actgtgaatg cgcaaaccaa cccttggcag aacatatcca 2700

2641 ggttagttaa gaacgcctct tgacacttac gcgtttggtt gggaaccgtc ttgtataggt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... MscI(2760)

2701 tcgcgtccgc catctccagc agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc 2760

2701 agcgcaggcg gtagaggtcg tcggcgtgcg ccgcgtagag cccgtcgcaa cccaggaccg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cacgggtgcg catgatcgtg ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc 2820

2761 gtgcccacgc gtactagcac gaggacagca actcctgggc cgatccgacc gccccaacgg 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttactggtta gcagaatgaa tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca 2880

2821 aatgaccaat cgtcttactt agtggctatg cgctcgcttg cacttcgctg acgacgacgt 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aaacgtctgc gacctgagca acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc 2940

2881 tttgcagacg ctggactcgt tgttgtactt accagaagcc aaaggcacaa agcatttcag 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tggaaacgcg gaagtcagcg ccctgcacca ttatgttccg gatctgcatc gcaggatgct 3000

2941 acctttgcgc cttcagtcgc gggacgtggt aatacaaggc ctagacgtag cgtcctacga 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gctggctacc ctgtggaaca cctacatctg tattaacgaa gcgctggcat tgaccctgag 3060

3001 cgaccgatgg gacaccttgt ggatgtagac ataattgctt cgcgaccgta actgggactc 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgatttttct ctggtcccgc cgcatccata ccgccagttg tttaccctca caacgttcca 3120

3061 actaaaaaga gaccagggcg gcgtaggtat ggcggtcaac aaatgggagt gttgcaaggt 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gtaaccgggc atgttcatca tcagtaaccc gtatcgtgag catcctctct cgtttcatcg 3180

3121 cattggcccg tacaagtagt agtcattggg catagcactc gtaggagaga gcaaagtagc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gtatcattac ccccatgaac agaaatcccc cttacacgga ggcatcagtg accaaacagg 3240

3181 catagtaatg ggggtacttg tctttagggg gaatgtgcct ccgtagtcac tggtttgtcc 3240

.......... .......... .......... .......... .......... ROP

.......... .......... .......... .......... .......... ..........

3241 aaaaaaccgc ccttaacatg gcccgcttta tcagaagcca gacattaacg cttctggaga 3300

3241 ttttttggcg ggaattgtac cgggcgaaat agtcttcggt ctgtaattgc gaagacctct 3300

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 aactcaacga gctggacgcg gatgaacagg cagacatctg tgaatcgctt cacgaccacg 3360

3301 ttgagttgct cgacctgcgc ctacttgtcc gtctgtagac acttagcgaa gtgctggtgc 3360

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctgatgagct ttaccgcagc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac 3420

3361 gactactcga aatggcgtcg acggagcgcg caaagccact actgccactt ttggagactg 3420

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag 3480

3421 tgtacgtcga gggcctctgc cagtgtcgaa cagacattcg cctacggccc tcgtctgttc 3480

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac 3540

3481 gggcagtccc gcgcagtcgc ccacaaccgc ccacagcccc gcgtcggtac tgggtcagtg 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gtagcgatag cggagtgtat actggcttaa ctatgcggca tcagagcaga ttgtactgag 3600

3541 catcgctatc gcctcacata tgaccgaatt gatacgccgt agtctcgtct aacatgactc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 agtgcaccat atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc 3660

3601 tcacgtggta tatacgccac actttatggc gtgtctacgc attcctcttt tatggcgtag 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga 3720

3661 tccgcgagaa ggcgaaggag cgagtgactg agcgacgcga gccagcaagc cgacgccgct 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca 3780

3721 cgccatagtc gagtgagttt ccgccattat gccaataggt gtcttagtcc cctattgcgt 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg 3840

3781 cctttcttgt acactcgttt tccggtcgtt ttccggtcct tggcattttt ccggcgcaac 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt 3900

3841 gaccgcaaaa aggtatccga ggcgggggga ctgctcgtag tgtttttagc tgcgagttca 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc 3960

3901 gtctccaccg ctttgggctg tcctgatatt tctatggtcc gcaaaggggg accttcgagg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct 4020

3961 gagcacgcga gaggacaagg ctgggacggc gaatggccta tggacaggcg gaaagaggga 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc 4080

4021 agcccttcgc accgcgaaag agtatcgagt gcgacatcca tagagtcaag ccacatccag 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta 4140

4081 caagcgaggt tcgacccgac acacgtgctt ggggggcaag tcgggctggc gacgcggaat 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca 4200

4141 aggccattga tagcagaact caggttgggc cattctgtgc tgaatagcgg tgaccgtcgt 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag 4260

4201 cggtgaccat tgtcctaatc gtctcgctcc atacatccgc cacgatgtct caagaacttc 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 tggtggccta actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag 4320

4261 accaccggat tgatgccgat gtgatcttcc tgtcataaac catagacgcg agacgacttc 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt 4380

4321 ggtcaatgga agcctttttc tcaaccatcg agaactaggc cgtttgtttg gtggcgacca 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa 4440

4381 tcgccaccaa aaaaacaaac gttcgtcgtc taatgcgcgt ctttttttcc tagagttctt 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg 4500

4441 ctaggaaact agaaaagatg ccccagactg cgagtcacct tgcttttgag tgcaattccc 4500

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga 4560

4501 taaaaccagt actctaatag tttttcctag aagtggatct aggaaaattt aatttttact 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta 4620

4561 tcaaaattta gttagatttc atatatactc atttgaacca gactgtcaat ggttacgaat 4620

.......... .......... .......... .......... ....... ORF 1 Ampicillin

.......... .......... .......... .......... .......... ..........

4621 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc 4680

4621 tagtcactcc gtggatagag tcgctagaca gataaagcaa gtaggtatca acggactgag 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg 4740

4681 gggcagcaca tctattgatg ctatgccctc ccgaatggta gaccggggtc acgacgttac 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga 4800

4741 tatggcgctc tgggtgcgag tggccgaggt ctaaatagtc gttatttggt cggtcggcct 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt 4860

4801 tcccggctcg cgtcttcacc aggacgttga aataggcgga ggtaggtcag ataattaaca 4860

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt 4920

4861 acggcccttc gatctcattc atcaagcggt caattatcaa acgcgttgca acaacggtaa 4920

.......... ....... ORF 1 Ampicillin ....... .......... ..........

PstI(4927) .......... .......... .......... .......... ..........

4921 gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc 4980

4921 cgacgtccgt agcaccacag tgcgagcagc aaaccatacc gaagtaagtc gaggccaagg 4980

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc 5040

4981 gttgctagtt ccgctcaatg tactaggggg tacaacacgt tttttcgcca atcgaggaag 5040

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca 5100

5041 ccaggaggct agcaacagtc ttcattcaac cggcgtcaca atagtgagta ccaataccgt 5100

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag 5160

5101 cgtgacgtat taagagaatg acagtacggt aggcattcta cgaaaagaca ctgaccactc 5160

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg 5220

5161 atgagttggt tcagtaagac tcttatcaca tacgccgctg gctcaacgag aacgggccgc 5220

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tcaacacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa 5280

5221 agttgtgccc tattatggcg cggtgtatcg tcttgaaatt ttcacgagta gtaacctttt 5280

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa 5340

5281 gcaagaagcc ccgcttttga gagttcctag aatggcgaca actctaggtc aagctacatt 5340

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga 5400

5341 gggtgagcac gtgggttgac tagaagtcgt agaaaatgaa agtggtcgca aagacccact 5400

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga 5460

5401 cgtttttgtc cttccgtttt acggcgtttt ttcccttatt cccgctgtgc ctttacaact 5460

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg 5520

5461 tatgagtatg agaaggaaaa agttataata acttcgtaaa tagtcccaat aacagagtac 5520

ORF 1 Ampicillin .... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

5521 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt 5580

5521 tcgcctatgt ataaacttac ataaatcttt ttatttgttt atccccaagg cgcgtgtaaa 5580

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ccccgaaaag tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa 5640

5581 ggggcttttc acggtggact gcagattctt tggtaataat agtactgtaa ttggatattt 5640

.......... .......... .......... .......... .......... ..........

.......... .......... ......... EcoRI(5675) ......... ..........

5641 aataggcgta tcacgaggcc ctttcgtctt caagaa.... .......... .......... 5676

5641 ttatccgcat agtgctccgg gaaagcagaa gttctt.... .......... .......... 5676

.......... .......... .......... .......... .......... ..........