)*LOCUS pET 11 c 5675 bp DNA SYN 11-Sep-2007 DEFINITION pET 11 c ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..5675 /organism="pET 11 c" /mol_type="other DNA" terminator complement(188..316) /label="T7_terminator" misc_feature 256..274 /label="T7_Terminal_primer" misc_feature complement(326..358) /label="T7_gene10_leader" misc_feature complement(326..358) /label="T7_leader" misc_feature complement(341..359) /label="Xpress_fwd_primer" misc_feature complement(367..383) /label="T7_transl_en_RBS" misc_feature complement(401..428) /label="lacO" promoter complement(428..446) /label="T7_promoter" misc_feature complement(530..549) /label="pBRrevBam_primer" misc_feature 824..1915 /label="lacI" CDS 956..1915 /label="ORF frame 2" /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*" CDS complement(1698..2393) /label="ORF frame 3" /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSRSRCLMSELTYINCVAL TARFPVGKPVVPAALMNRPTRGERRFAYWAPGWFFFSPVRRATADCPSPPGPERVAAS GPRWFAPAGENPV*" CDS 1971..2588 /label="ORF frame 3" /translation="MPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVI FGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVL LAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITG PLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*" gene complement(1974..2588) /label="tet(1191-576)" /gene="tet(1191-576)" misc_feature 3227..3418 /label="ROP" misc_feature complement(3412..3434) /label="pGEX_3_primer" rep_origin complement(3833..4452) /label="pBR322_origin" CDS complement(4607..5467) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4607..5467) /label="Ampicillin" /gene="Ampicillin" promoter complement(5509..5537) /label="AmpR_promoter" ORIGIN 1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 301 cgggctttgt tagcagccgg atccgaccca tttgctgtcc accagtcatg ctagccatat 361 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca 421 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc 481 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 541 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 601 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca 661 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg 721 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt 781 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc 841 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 901 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 961 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 1021 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 1081 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 1141 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 1201 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 1261 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 1321 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1381 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1441 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1501 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1561 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1621 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1681 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1741 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1801 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1861 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 1921 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga 1981 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 2041 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca 2101 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat 2161 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg 2221 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg 2281 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca 2341 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac 2401 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg 2461 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg 2521 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct 2581 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc 2641 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat 2701 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc 2761 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct 2821 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa 2881 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct 2941 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg 3001 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt 3061 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag 3121 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg 3181 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga 3241 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa 3301 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc 3361 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 3541 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 3601 gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 3661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 3721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 3781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 3841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 3901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 3961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 4021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 4081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 4141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 4201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 4261 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 4321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 4381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 4441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 4501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 4561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 4621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 4681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 4741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 4801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 4861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 4921 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 4981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 5041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 5101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 5161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 5221 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 5281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 5341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 5401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 5461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 5521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 5581 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 5641 ataggcgtat cacgaggccc tttcgtcttc aagaa //D pET 11 c "Times New RomanCD<T7_term"Times New RomanCDT7_Terminal_pri"Times New RomanCɆDFfT7_gene10_leader"Times New RomanDFf T7_leader"Times New RomanCDUgXpress_fwd_pri"Times New Roman*CDoT7_transl_en_RBS"Times New RomanCɆDlacO"Times New RomanDT7_pro"Times New RomanCD% pBRrevBam_pri"Times New RomanCD8{lacI"Times New RomanCɆD{ORF 2"Times New RomanDY ORF 3"Times New Roman*CD ORF 3"Times New RomanCD  tet(1191-576)"Times New RomanCɆD Z ROP"Times New RomanDT j  pGEX_3_pri"Times New RomanCDd pBR322_origin"Times New RomanCD[ORF 2"Times New RomanCɆD[ Ampicillin"Times New Roman(DAmpR_pro"Times New Roman ClaI(24),HindIII(29)"Times New RomanD{ 3BamHI(319),NheI(350),NdeI(357),XbaI(395),BglII(461)"Times New Roman? 3Da BclI"Times New RomanD|yr ApaI"Times New Romanr D HpaI"Times New RomanD EagI(2251),NruI(2286)"Times New RomanDCpIxOO MscI"Times New Roman D PstI"Times New Roman=D$ AatII(5602),EcoRI(5673)"Times New RomanDs test"Times New RomanpD x