Sequence of pET_11

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Sequence of pET_11_c

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Sequence of pET 11 c : 5675 BP; 1299 A; 1549 C; 1495 G; 1332 T; 0 other;

.......... ..... ClaI(24),HindIII(29) ..... .......... ..........

1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 60

1 aagagtacaa actgtcgaat agtagctatt cgaaattacg ccatcaaata gtgtcaattt 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 120

61 aacgattgcg tcagtccgtg gcacatactt tagattgtta cgcgagtagc agtaggagcc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 180

121 gtggcagtgg gacctacgac atccgtatcc gaaccaatac ggccatgacg gcccggagaa 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 240

181 cgccctatag gcctatatca aggaggaaag tcgttttttg gggagttctg ggcaaatctc 240

.......... .......... .......... T7_term .......... ..........

.......... .......... .......... .......... .......... ..........

241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 300

241 cggggttccc caatacgatc aataacgagt cgccaccgtc gtcggttgag tcgaaggaaa 300

.......... ... T7_term T7_Terminal_pri ... .......... ..........

.......... BamHI(319) .......... .......... NheI(350) ..........

301 cgggctttgt tagcagccgg atccgaccca tttgctgtcc accagtcatg ctagccatat 360

301 gcccgaaaca atcgtcggcc taggctgggt aaacgacagg tggtcagtac gatcggtata 360

T7_term .......... .... T7_gene10_leader T7_leader Xpress_fwd_pri

.......... .......... .......... .......... .......... ..........

361 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca 420

361 catatagagg aagaatttca atttgtttta ataaagatct ccccttaaca ataggcgagt 420

....... T7_transl_en_RBS ....... .......... lacO ..........

.......... .......... .......... .......... BglII(461) ..........

421 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc 480

421 gttaagggga tatcactcag cataattaaa gcgccctagc tctagagcta ggagatgcgg 480

.......... T7_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 540

481 cctgcgtagc accggccgta gtggccgcgg tgtccacgcc aacgaccgcg gatatagcgg 540

.......... .......... .......... .......... ........ pBRrevBam_pri

.......... .......... .......... .......... .......... ..........

541 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 600

541 ctgtagtggc taccccttct agcccgagcg gtgaagcccg agtactcgcg aacaaagccg 600

pBRrevBam_pri ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca 660

601 cacccatacc accgtccggg gcaccggccc cctgacaacc cgcggtagag gaacgtacgt 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg 720

661 ggtaaggaac gccgccgcca cgagttgccg gagttggatg atgacccgac gaaggattac 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt 780

721 gtcctcagcg tattccctct cgcagctcta gggcctgtgg tagcttaccg cgttttggaa 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc 840

781 agcgccatac cgtactatcg cgggccttct ctcagttaag tcccaccact tacactttgg 840

.......... .......... .......... .......... .......... lacI

.......... .......... .......... .......... .......... ..........

841 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 900

841 tcattgcaat atgctacagc gtctcatacg gccacagaga atagtctggc aaagggcgca 900

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 960

901 ccacttggtc cggtcggtgc aaagacgctt ttgcgccctt tttcaccttc gccgctaccg 960

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 1020

961 cctcgactta atgtaagggt tggcgcaccg tgttgttgac cgcccgtttg tcagcaacga 1020

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 1080

1021 ctaaccgcaa cggtggaggt cagaccggga cgtgcgcggc agcgtttaac agcgccgcta 1080

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 1140

1081 atttagagcg cggctagttg acccacggtc gcaccaccac agctaccatc ttgcttcgcc 1140

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... BclI(1197)

1141 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 1200

1141 gcagcttcgg acatttcgcc gccacgtgtt agaagagcgc gttgcgcagt cacccgacta 1200

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 1260

1201 gtaattgata ggcgacctac tggtcctacg gtaacgacac cttcgacgga cgtgattaca 1260

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 1320

1261 aggccgcaat aaagaactac agagactggt ctgtgggtag ttgtcataat aaaagagggt 1320

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1380

1321 acttctgcca tgcgctgacc cgcacctcgt agaccagcgt aacccagtgg tcgtttagcg 1380

.......... .......... lacI ORF 2 .......... .......... ..........

.......... ApaI(1394) .......... .......... .......... ..........

1381 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1440

1381 cgacaatcgc ccgggtaatt caagacagag ccgcgcagac gcagaccgac cgaccgtatt 1440

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1500

1441 tatagagtga gcgttagttt aagtcggcta tcgccttgcc cttccgctga cctcacggta 1500

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1560

1501 caggccaaaa gttgtttggt acgtttacga cttactcccg tagcaagggt gacgctacga 1560

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1620

1561 ccaacggttg ctagtctacc gcgacccgcg ttacgcgcgg taatggctca ggcccgacgc 1620

.......... .......... lacI ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1680

1621 gcaaccacgc ctatagagcc atcaccctat gctgctatgg cttctgtcga gtacaatata 1680

.......... .......... lacI ORF 2 .......... .......... ..........

HpaI(1689) .......... .......... .......... .......... ..........

1681 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1740

1681 gggcggcaat tggtggtagt ttgtcctaaa agcggacgac cccgtttggt cgcacctggc 1740

.......... .......... lacI ORF 2 ORF 3 .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1800

1741 gaacgacgtt gagagagtcc cggtccgcca cttcccgtta gtcgacaacg ggcagagtga 1800

.......... ....... lacI ORF 2 ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1860

1801 ccacttttct ttttggtggg accgcgggtt atgcgtttgg cggagagggg cgcgcaaccg 1860

.......... ....... lacI ORF 2 ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 1920

1861 gctaagtaat tacgtcgacc gtgctgtcca aagggctgac ctttcgcccg tcactcgcgt 1920

.......... ....... lacI ORF 2 ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga 1980

1921 tgcgttaatt acattcaatc gagtgagtaa tccgtggccc tagagctggc tacgggaact 1980

.......... .......... ORF 3 .......... .......... ORF 3

.......... .......... .......... .......... .......... ..........

1981 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 2040

1981 ctcggaagtt gggtcagtcg aggaaggcca cccgcgcccc gtactgatag cagcggcgtg 2040

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca 2100

2041 aatactgaca gaagaaatag tacgttgagc atcctgtcca cggccgtcgc gagacccagt 2100

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat 2160

2101 aaaagccgct cctggcgaaa gcgacctcgc gctgctacta gccggacagc gaacgccata 2160

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg 2220

2161 agccttagaa cgtgcgggag cgagttcgga agcagtgacc agggcggtgg tttgcaaagc 2220

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg 2280

2221 cgctcttcgt ccggtaatag cggccgtacc gccggctgcg cgacccgatg cagaacgacc 2280

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

NruI(2286) .......... .......... .......... .......... ..........

2281 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca 2340

2281 gcaagcgctg cgctccgacc taccggaagg ggtaatacta agaagagcga aggccgccgt 2340

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac 2400

2341 agccctacgg gcgcaacgtc cggtacgaca ggtccgtcca tctactgctg gtagtccctg 2400

.......... .. ORF 3 ORF 3 tet(1191-576) .. .......... ..........

.......... .......... .......... .......... .......... ..........

2401 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg 2460

2401 tcgaagttcc tagcgagcgc cgagaatggt cggattgaag ctagtgacct ggcgactagc 2460

.......... ..... ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg 2520

2461 agtgccgcta aatacggcgg agccgctcgt gtaccttgcc caaccgtacc taacatccgc 2520

.......... ..... ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct 2580

2521 ggcgggatat ggaacagacg gaggggcgca acgcagcgcc acgtacctcg gcccggtgga 2580

.......... ..... ORF 3 tet(1191-576) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc 2640

2581 gctggactta ccttcggccg ccgtggagcg attgcctaag tggtgaggtt cttaacctcg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat 2700

2641 gttagttaag aacgcctctt gacacttacg cgtttggttg ggaaccgtct tgtataggta 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... MscI(2758)

2701 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc 2760

2701 gcgcaggcgg tagaggtcgt cggcgtgcgc cgcgtagagc ccgtcgcaac ccaggaccgg 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct 2820

2761 tgcccacgcg tactagcacg aggacagcaa ctcctgggcc gatccgaccg ccccaacgga 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa 2880

2821 atgaccaatc gtcttactta gtggctatgc gctcgcttgc acttcgctga cgacgacgtt 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct 2940

2881 ttgcagacgc tggactcgtt gttgtactta ccagaagcca aaggcacaaa gcatttcaga 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg 3000

2941 cctttgcgcc ttcagtcgcg ggacgtggta atacaaggcc tagacgtagc gtcctacgac 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt 3060

3001 gaccgatggg acaccttgtg gatgtagaca taattgcttc gcgaccgtaa ctgggactca 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag 3120

3061 ctaaaaagag accagggcgg cgtaggtatg gcggtcaaca aatgggagtg ttgcaaggtc 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg 3180

3121 attggcccgt acaagtagta gtcattgggc atagcactcg taggagagag caaagtagcc 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga 3240

3181 atagtaatgg gggtacttgt ctttaggggg aatgtgcctc cgtagtcact ggtttgtcct 3240

.......... .......... .......... .......... .......... ROP

.......... .......... .......... .......... .......... ..........

3241 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa 3300

3241 tttttggcgg gaattgtacc gggcgaaata gtcttcggtc tgtaattgcg aagacctctt 3300

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc 3360

3301 tgagttgctc gacctgcgcc tacttgtccg tctgtagaca cttagcgaag tgctggtgcg 3360

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 3420

3361 actactcgaa atggcgtcga cggagcgcgc aaagccacta ctgccacttt tggagactgt 3420

.......... .......... ROP .......... .......... pGEX_3_pri

.......... .......... .......... .......... .......... ..........

3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 3480

3421 gtacgtcgag ggcctctgcc agtgtcgaac agacattcgc ctacggccct cgtctgttcg 3480

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 3540

3481 ggcagtcccg cgcagtcgcc cacaaccgcc cacagccccg cgtcggtact gggtcagtgc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 3600

3541 atcgctatcg cctcacatat gaccgaattg atacgccgta gtctcgtcta acatgactct 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gtgcaccata tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 3660

3601 cacgtggtat atacgccaca ctttatggcg tgtctacgca ttcctctttt atggcgtagt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 3720

3661 ccgcgagaag gcgaaggagc gagtgactga gcgacgcgag ccagcaagcc gacgccgctc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 3780

3721 gccatagtcg agtgagtttc cgccattatg ccaataggtg tcttagtccc ctattgcgtc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 3840

3781 ctttcttgta cactcgtttt ccggtcgttt tccggtcctt ggcatttttc cggcgcaacg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 3900

3841 accgcaaaaa ggtatccgag gcggggggac tgctcgtagt gtttttagct gcgagttcag 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 3960

3901 tctccaccgc tttgggctgt cctgatattt ctatggtccg caaaggggga ccttcgaggg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 4020

3961 agcacgcgag aggacaaggc tgggacggcg aatggcctat ggacaggcgg aaagagggaa 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 4080

4021 gcccttcgca ccgcgaaaga gtatcgagtg cgacatccat agagtcaagc cacatccagc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 4140

4081 aagcgaggtt cgacccgaca cacgtgcttg gggggcaagt cgggctggcg acgcggaata 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 4200

4141 ggccattgat agcagaactc aggttgggcc attctgtgct gaatagcggt gaccgtcgtc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 4260

4201 ggtgaccatt gtcctaatcg tctcgctcca tacatccgcc acgatgtctc aagaacttca 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 4320

4261 ccaccggatt gatgccgatg tgatcttcct gtcataaacc atagacgcga gacgacttcg 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 4380

4321 gtcaatggaa gcctttttct caaccatcga gaactaggcc gtttgtttgg tggcgaccat 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 4440

4381 cgccaccaaa aaaacaaacg ttcgtcgtct aatgcgcgtc tttttttcct agagttcttc 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 4500

4441 taggaaacta gaaaagatgc cccagactgc gagtcacctt gcttttgagt gcaattccct 4500

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 4560

4501 aaaaccagta ctctaatagt ttttcctaga agtggatcta ggaaaattta atttttactt 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 4620

4561 caaaatttag ttagatttca tatatactca tttgaaccag actgtcaatg gttacgaatt 4620

.......... .......... .......... .......... ....... ORF 2 Ampicillin

.......... .......... .......... .......... .......... ..........

4621 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 4680

4621 agtcactccg tggatagagt cgctagacag ataaagcaag taggtatcaa cggactgagg 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 4740

4681 ggcagcacat ctattgatgc tatgccctcc cgaatggtag accggggtca cgacgttact 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 4800

4741 atggcgctct gggtgcgagt ggccgaggtc taaatagtcg ttatttggtc ggtcggcctt 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 4860

4801 cccggctcgc gtcttcacca ggacgttgaa ataggcggag gtaggtcaga taattaacaa 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 4920

4861 cggcccttcg atctcattca tcaagcggtc aattatcaaa cgcgttgcaa caacggtaac 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

PstI(4925) .......... .......... .......... .......... ..........

4921 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 4980

4921 gacgtccgta gcaccacagt gcgagcagca aaccataccg aagtaagtcg aggccaaggg 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 5040

4981 ttgctagttc cgctcaatgt actagggggt acaacacgtt ttttcgccaa tcgaggaagc 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 5100

5041 caggaggcta gcaacagtct tcattcaacc ggcgtcacaa tagtgagtac caataccgtc 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 5160

5101 gtgacgtatt aagagaatga cagtacggta ggcattctac gaaaagacac tgaccactca 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 5220

5161 tgagttggtt cagtaagact cttatcacat acgccgctgg ctcaacgaga acgggccgca 5220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 5280

5221 gttgtgccct attatggcgc ggtgtatcgt cttgaaattt tcacgagtag taaccttttg 5280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 5340

5281 caagaagccc cgcttttgag agttcctaga atggcgacaa ctctaggtca agctacattg 5340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 5400

5341 ggtgagcacg tgggttgact agaagtcgta gaaaatgaaa gtggtcgcaa agacccactc 5400

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 5460

5401 gtttttgtcc ttccgtttta cggcgttttt tcccttattc ccgctgtgcc tttacaactt 5460

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 5520

5461 atgagtatga gaaggaaaaa gttataataa cttcgtaaat agtcccaata acagagtact 5520

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

5521 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 5580

5521 cgcctatgta taaacttaca taaatctttt tatttgttta tccccaaggc gcgtgtaaag 5580

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 5640

5581 gggcttttca cggtggactg cagattcttt ggtaataata gtactgtaat tggatatttt 5640

.......... .......... .......... .......... .......... ..........

.......... .......... ......... EcoRI(5673) ......... ..........

5641 ataggcgtat cacgaggccc tttcgtcttc aagaa..... .......... .......... 5675

5641 tatccgcata gtgctccggg aaagcagaag ttctt..... .......... .......... 5675

.......... .......... .......... .......... .......... ..........