Sequence of pET_DEST42

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Sequence of pET_DEST42_Gateway

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Sequence of pET DEST42 Gateway : 7440 BP; 1825 A; 1866 C; 1955 G; 1794 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gatggtgtcc gggatctcga cgctctccct tatgcgactc ctgcattagg aagcagccca 60

1 ctaccacagg ccctagagct gcgagaggga atacgctgag gacgtaatcc ttcgtcgggt 60

.......... .......... .......... ......... tet(636-888) .........

.......... .......... .......... .......... .......... ..........

61 gtagtaggtt gaggccgttg agcaccgccg ccgcaaggaa tggtgcatgc aaggagatgg 120

61 catcatccaa ctccggcaac tcgtggcggc ggcgttcctt accacgtacg ttcctctacc 120

.......... ......... tet(636-888) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cgcccaacag tcccccggcc acggggcctg ccaccatacc cacgccgaaa caagcgctca 180

121 gcgggttgtc agggggccgg tgccccggac ggtggtatgg gtgcggcttt gttcgcgagt 180

.......... ......... tet(636-888) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tgagcccgaa gtggcgagcc cgatcttccc catcggtgat gtcggcgata ta ggcgccag 240

181 actcgggctt caccgctcgg gctagaaggg gtagccacta cagccgctat atccgcggtc 240

.......... ......... tet(636-888) ....... pBRrevBam_pri ........

.......... .......... .......... .......... .......... ..........

241 caaccgcacc tgtggcgccg gtgatgccgg ccacgatgcg tccggcgtag aggatcgaga 300

241 gttggcgtgg acaccgcggc cactacggcc ggtgctacgc aggccgcatc tcctagctct 300

.......... ......... tet(636-888) ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tctcgatccc gcgaaattaa tacgactcac tataggggaa ttgtgagcgg ataacaattc 360

301 agagctaggg cgctttaatt atgctgagtg atatcccctt aacactcgcc tattgttaag 360

.......... .......... T7_pro .......... lacO ..........

XbaI(364) .......... .......... .......... .......... ..........

361 ccctctagaa ataattttgt ttaactttaa gaaggaatat cacaagtttg tacaaaaaag 420

361 gggagatctt tattaaaaca aattgaaatt cttccttata gtgttcaaac atgttttttc 420

.......... .......... .......... .......... .......... attR1

.......... .......... .......... .......... .......... ..........

421 ctgaacgaga aacgtaaaat gatataaata tcaatatatt aaattagatt ttgcataaaa 480

421 gacttgctct ttgcatttta ctatatttat agttatataa tttaatctaa aacgtatttt 480

.......... .......... attR1 attR2 .......... ..........

.......... .......... .......... .......... NotI(528) ..........

481 aacagactac ataatactgt aaaacacaac atatccagtc actatggcgg ccgcattagg 540

481 ttgtctgatg tattatgaca ttttgtgttg tataggtcag tgataccgcc ggcgtaatcc 540

.......... ......... attR1 attR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 caccccaggc tttacacttt atgcttccgg ctcgtataat gtgtggattt tgagttagga 600

541 gtggggtccg aaatgtgaaa tacgaaggcc gagcatatta cacacctaaa actcaatcct 600

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tccggcgaga ttttcaggag ctaaggaagc taaaatggag aaaaaaatca ctggatatac 660

601 aggccgctct aaaagtcctc gattccttcg attttacctc tttttttagt gacctatatg 660

.......... .......... .......... ........ ORF 2 CAT/CamR ........

.......... .......... .......... .......... .......... ..........

661 caccgttgat atatcccaat ggcatcgtaa agaacatttt gaggcatttc agtcagttgc 720

661 gtggcaacta tatagggtta ccgtagcatt tcttgtaaaa ctccgtaaag tcagtcaacg 720

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 tcaatgtacc tataaccaga ccgttcagct ggatattacg gcctttttaa agaccgtaaa 780

721 agttacatgg atattggtct ggcaagtcga cctataatgc cggaaaaatt tctggcattt 780

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 gaaaaataag cacaagtttt atccggcctt tattcacatt cttgcccgcc tgatgaatgc 840

781 ctttttattc gtgttcaaaa taggccggaa ataagtgtaa gaacgggcgg actacttacg 840

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

EcoRI(848) .......... .......... .......... .......... ..........

841 tcatccggaa ttccgtatgg caatgaaaga cggtgagctg gtgatatggg atagtgttca 900

841 agtaggcctt aaggcatacc gttactttct gccactcgac cactataccc tatcacaagt 900

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 cccttgttac accgttttcc atgagcaaac tgaaacgttt tcatcgctct ggagtgaata 960

901 gggaacaatg tggcaaaagg tactcgtttg actttgcaaa agtagcgaga cctcacttat 960

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 ccacgacgat ttccggcagt ttctacacat atattcgcaa gatgtggcgt gttacggtga 1020

961 ggtgctgcta aaggccgtca aagatgtgta tataagcgtt ctacaccgca caatgccact 1020

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aaacctggcc tatttcccta aagggtttat tgagaatatg tttttcgtct cagccaatcc 1080

1021 tttggaccgg ataaagggat ttcccaaata actcttatac aaaaagcaga gtcggttagg 1080

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ctgggtgagt ttcaccagtt ttgatttaaa cgtggccaat atggacaact tcttcgcccc 1140

1081 gacccactca aagtggtcaa aactaaattt gcaccggtta tacctgttga agaagcgggg 1140

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

NcoI(1149) .......... .......... .......... .......... ..........

1141 cgttttcacc atgggcaaat attatacgca aggcgacaag gtgctgatgc cgctggcgat 1200

1141 gcaaaagtgg tacccgttta taatatgcgt tccgctgttc cacgactacg gcgaccgcta 1200

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tcaggttcat catgccgtct gtgatggctt ccatgtcggc agaatgctta atgaattaca 1260

1201 agtccaagta gtacggcaga cactaccgaa ggtacagccg tcttacgaat tacttaatgt 1260

.......... ........ ORF 2 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1261 acagtactgc gatgagtggc agggcggggc gtaaacgcgt ggatccggct tactaaaagc 1320

1261 tgtcatgacg ctactcaccg tcccgccccg catttgcgca cctaggccga atgattttcg 1320

........ ORF 2 CAT/CamR ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cagataacag tatgcgtatt tgcgcgctga tttttgcggt ataagaatat atactgatat 1380

1321 gtctattgtc atacgcataa acgcgcgact aaaaacgcca tattcttata tatgactata 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtatacccga agtatgtcaa aaagaggtgt gctatgaagc agcgtattac agtgacagtt 1440

1381 catatgggct tcatacagtt tttctccaca cgatacttcg tcgcataatg tcactgtcaa 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gacagcgaca gctatcagtt gctcaaggca tatatgatgt caatatctcc ggtctggtaa 1500

1441 ctgtcgctgt cgatagtcaa cgagttccgt atatactaca gttatagagg ccagaccatt 1500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gcacaaccat gcagaatgaa gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc 1560

1501 cgtgttggta cgtcttactt cgggcagcag acgcacggct tgcgaccttt cgccttttag 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aggaagggat ggctgaggtc gcccggttta ttgaaatgaa cggctctttt gctgacgaga 1620

1561 tccttcccta ccgactccag cgggccaaat aactttactt gccgagaaaa cgactgctct 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 acagggactg gtgaaatgca gtttaaggtt tacacctata aaagagagag ccgttatcgt 1680

1621 tgtccctgac cactttacgt caaattccaa atgtggatat tttctctctc ggcaatagca 1680

.......... .......... .......... ccdB .......... ..........

.......... .......... .......... SmaI(1719) .......... ..........

1681 ctgtttgtgg atgtacagag tgatattatt gacacgcccg ggcgacggat ggtgatcccc 1740

1681 gacaaacacc tacatgtctc actataataa ctgtgcgggc ccgctgccta ccactagggg 1740

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctggccagtg cacgtctgct gtcagataaa gtctcccgtg aactttaccc ggtggtgcat 1800

1741 gaccggtcac gtgcagacga cagtctattt cagagggcac ttgaaatggg ccaccacgta 1800

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atcggggatg aaagctggcg catgatgacc accgatatgg ccagtgtgcc ggtctccgtt 1860

1801 tagcccctac tttcgaccgc gtactactgg tggctatacc ggtcacacgg ccagaggcaa 1860

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 atcggggaag aagtggctga tctcagccac cgcgaaaatg acatcaaaaa cgccattaac 1920

1861 tagccccttc ttcaccgact agagtcggtg gcgcttttac tgtagttttt gcggtaattg 1920

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ctgatgttct ggggaatata aatgtcaggc tccgttatac acagccagtc tgcaggtcga 1980

1921 gactacaaga ccccttatat ttacagtccg aggcaatatg tgtcggtcag acgtccagct 1980

.......... ccdB .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 ccatagtgac tggatatgtt gtgttttaca gtattatgta gtctgttttt tatgcaaaat 2040

1981 ggtatcactg acctatacaa cacaaaatgt cataatacat cagacaaaaa atacgtttta 2040

.......... .......... ......... attR2 attR1 ......... ..........

.......... .......... .......... .......... .......... ..........

2041 ctaatttaat atattgatat ttatatcatt ttacgtttct cgttcagctt tcttgtacaa 2100

2041 gattaaatta tataactata aatatagtaa aatgcaaaga gcaagtcgaa agaacatgtt 2100

.......... ......... attR2 attR1 ......... .......... ..........

......... BstBI(2114) ......... .......... .......... ..........

2101 agtggtgatc aattcgaagc ttgaaggtaa gcctatccct aaccctctcc tcggtctcga 2160

2101 tcaccactag ttaagcttcg aacttccatt cggataggga ttgggagagg agccagagct 2160

.......... .......... .......... .......... V5 ..........

.......... AgeI(2171) .......... .......... .......... ..........

2161 ttctacgcgt accggtcatc atcaccatca ccattgagtt tgatccggct gctaacaaag 2220

2161 aagatgcgca tggccagtag tagtggtagt ggtaactcaa actaggccga cgattgtttc 2220

.......... .......... 6xHis .......... .......... T7_term

.......... .......... .......... .......... .......... ..........

2221 cccgaaagga agctgagttg gctgctgcca ccgctgagca ataactagca taaccccttg 2280

2221 gggctttcct tcgactcaac cgacgacggt ggcgactcgt tattgatcgt attggggaac 2280

.......... ....... T7_term T7_Terminal_pri ....... ..........

.......... .......... .......... .......... .......... ..........

2281 gggcctctaa acgggtcttg aggggttttt tgctgaaagg aggaactata tccggatatc 2340

2281 cccggagatt tgcccagaac tccccaaaaa acgactttcc tccttgatat aggcctatag 2340

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccgcaagagg cccggcagta ccggcataac caagcctatg cctacagcat ccagggtgac 2400

2341 ggcgttctcc gggccgtcat ggccgtattg gttcggatac ggatgtcgta ggtcccactg 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ggtgccgagg atgacgatga gcgcattgtt agatttcata cacggtgcct gactgcgtta 2460

2401 ccacggctcc tactgctact cgcgtaacaa tctaaagtat gtgccacgga ctgacgcaat 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gcaatttaac tgtgataaac taccgcatta aagcttatcg atgataagct gtcaaacatg 2520

2461 cgttaaattg acactatttg atggcgtaat ttcgaatagc tactattcga cagtttgtac 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agaattaatt cttgaagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc 2580

2521 tcttaattaa gaacttctgc tttcccggag cactatgcgg ataaaaatat ccaattacag 2580

.......... .......... .......... .......... .......... ..........

.......... ......... AatII(2605) ......... .......... ..........

2581 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc 2640

2581 tactattatt accaaagaat ctgcagtcca ccgtgaaaag cccctttaca cgcgccttgg 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc 2700

2641 ggataaacaa ataaaaagat ttatgtaagt ttatacatag gcgagtactc tgttattggg 2700

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

2701 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc 2760

2701 actatttacg aagttattat aactttttcc ttctcatact cataagttgt aaaggcacag 2760

.......... .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

2761 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg 2820

2761 cgggaataag ggaaaaaacg ccgtaaaacg gaaggacaaa aacgagtggg tctttgcgac 2820

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat 2880

2821 cactttcatt ttctacgact tctagtcaac ccacgtgctc acccaatgta gcttgaccta 2880

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc 2940

2881 gagttgtcgc cattctagga actctcaaaa gcggggcttc ttgcaaaagg ttactactcg 2940

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 acttttaaag ttctgctatg tggcgcggta ttatcccgtg ttgacgccgg gcaagagcaa 3000

2941 tgaaaatttc aagacgatac accgcgccat aatagggcac aactgcggcc cgttctcgtt 3000

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa 3060

3001 gagccagcgg cgtatgtgat aagagtctta ctgaaccaac tcatgagtgg tcagtgtctt 3060

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt 3120

3061 ttcgtagaat gcctaccgta ctgtcattct cttaatacgt cacgacggta ttggtactca 3120

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct 3180

3121 ctattgtgac gccggttgaa tgaagactgt tgctagcctc ctggcttcct cgattggcga 3180

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat 3240

3181 aaaaacgtgt tgtaccccct agtacattga gcggaactag caacccttgg cctcgactta 3240

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gaagccatac caaacgacga gcgtgacacc acgatgcctg cagcaatggc aacaacgttg 3300

3241 cttcggtatg gtttgctgct cgcactgtgg tgctacggac gtcgttaccg ttgttgcaac 3300

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg 3360

3301 gcgtttgata attgaccgct tgatgaatga gatcgaaggg ccgttgttaa ttatctgacc 3360

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt 3420

3361 tacctccgcc tatttcaacg tcctggtgaa gacgcgagcc gggaaggccg accgaccaaa 3420

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg 3480

3421 taacgactat ttagacctcg gccactcgca cccagagcgc catagtaacg tcgtgacccc 3480

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg 3540

3481 ggtctaccat tcgggagggc atagcatcaa tagatgtgct gcccctcagt ccgttgatac 3540

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg 3600

3541 ctacttgctt tatctgtcta gcgactctat ccacggagtg actaattcgt aaccattgac 3600

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 3660

3601 agtctggttc aaatgagtat atatgaaatc taactaaatt ttgaagtaaa aattaaattt 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 3720

3661 tcctagatcc acttctagga aaaactatta gagtactggt tttagggaat tgcactcaaa 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 3780

3721 agcaaggtga ctcgcagtct ggggcatctt ttctagtttc ctagaagaac tctaggaaaa 3780

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3781 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 3840

3781 aaagacgcgc attagacgac gaacgtttgt ttttttggtg gcgatggtcg ccaccaaaca 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 3900

3841 aacggcctag ttctcgatgg ttgagaaaaa ggcttccatt gaccgaagtc gtctcgcgtc 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta 3960

3901 tatggtttat gacaggaaga tcacatcggc atcaatccgg tggtgaagtt cttgagacat 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 4020

3961 cgtggcggat gtatggagcg agacgattag gacaatggtc accgacgacg gtcaccgcta 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 4080

4021 ttcagcacag aatggcccaa cctgagttct gctatcaatg gcctattccg cgtcgccagc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 4140

4081 ccgacttgcc ccccaagcac gtgtgtcggg tcgaacctcg cttgctggat gtggcttgac 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 4200

4141 tctatggatg tcgcactcga tactctttcg cggtgcgaag ggcttccctc tttccgcctg 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 4260

4201 tccataggcc attcgccgtc ccagccttgt cctctcgcgt gctccctcga aggtccccct 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 4320

4261 ttgcggacca tagaaatatc aggacagccc aaagcggtgg agactgaact cgcagctaaa 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 4380

4321 aacactacga gcagtccccc cgcctcggat acctttttgc ggtcgttgcg ccggaaaaat 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 4440

4381 gccaaggacc ggaaaacgac cggaaaacga gtgtacaaga aaggacgcaa taggggacta 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg 4500

4441 agacacctat tggcataatg gcggaaactc actcgactat ggcgagcggc gtcggcttgc 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcctgatgcg gtattttctc 4560

4501 tggctcgcgt cgctcagtca ctcgctcctt cgccttctcg cggactacgc cataaaagag 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cttacgcatc tgtgcggtat ttcacaccgc atatatggtg cactctcagt acaatctgct 4620

4561 gaatgcgtag acacgccata aagtgtggcg tatataccac gtgagagtca tgttagacga 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ctgatgccgc atagttaagc cagtatacac tccgctatcg ctacgtgact gggtcatggc 4680

4621 gactacggcg tatcaattcg gtcatatgtg aggcgatagc gatgcactga cccagtaccg 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tgcgccccga cacccgccaa cacccgctga cgcgccctga cgggcttgtc tgctcccggc 4740

4681 acgcggggct gtgggcggtt gtgggcgact gcgcgggact gcccgaacag acgagggccg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 atccgcttac agacaagctg tgaccgtctc cgggagctgc atgtgtcaga ggttttcacc 4800

4741 taggcgaatg tctgttcgac actggcagag gccctcgacg tacacagtct ccaaaagtgg 4800

.......... .......... .......... .......... pGEX_3_pri ROP

.......... .......... .......... .......... .......... ..........

4801 gtcatcaccg aaacgcgcga ggcagctgcg gtaaagctca tcagcgtggt cgtgaagcga 4860

4801 cagtagtggc tttgcgcgct ccgtcgacgc catttcgagt agtcgcacca gcacttcgct 4860

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttcacagatg tctgcctgtt catccgcgtc cagctcgttg agtttctcca gaagcgttaa 4920

4861 aagtgtctac agacggacaa gtaggcgcag gtcgagcaac tcaaagaggt cttcgcaatt 4920

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgtctggctt ctgataaagc gggccatgtt aagggcggtt ttttcctgtt tggtcactga 4980

4921 acagaccgaa gactatttcg cccggtacaa ttcccgccaa aaaaggacaa accagtgact 4980

.......... .......... ROP .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 tgcctccgtg taagggggat ttctgttcat gggggtaatg ataccgatga aacgagagag 5040

4981 acggaggcac attcccccta aagacaagta cccccattac tatggctact ttgctctctc 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gatgctcacg atacgggtta ctgatgatga acatgcccgg ttactggaac gttgtgaggg 5100

5041 ctacgagtgc tatgcccaat gactactact tgtacgggcc aatgaccttg caacactccc 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 taaacaactg gcggtatgga tgcggcggga ccagagaaaa atcactcagg gtcaatgcca 5160

5101 atttgttgac cgccatacct acgccgccct ggtctctttt tagtgagtcc cagttacggt 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gcgcttcgtt aatacagatg taggtgttcc acagggtagc cagcagcatc ctgcgatgca 5220

5161 cgcgaagcaa ttatgtctac atccacaagg tgtcccatcg gtcgtcgtag gacgctacgt 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gatccggaac ataatggtgc agggcgctga cttccgcgtt tccagacttt acgaaacacg 5280

5221 ctaggccttg tattaccacg tcccgcgact gaaggcgcaa aggtctgaaa tgctttgtgc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gaaaccgaag accattcatg ttgttgctca ggtcgcagac gttttgcagc agcagtcgct 5340

5281 ctttggcttc tggtaagtac aacaacgagt ccagcgtctg caaaacgtcg tcgtcagcga 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 tcacgttcgc tcgcgtatcg gtgattcatt ctgctaacca gtaaggcaac cccgccagcc 5400

5341 agtgcaagcg agcgcatagc cactaagtaa gacgattggt cattccgttg gggcggtcgg 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tagccgggtc ctcaacgaca ggagcacgat catgcgcacc cgtggccagg acccaacgct 5460

5401 atcggcccag gagttgctgt cctcgtgcta gtacgcgtgg gcaccggtcc tgggttgcga 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gcccgagatg cgccgcgtgc ggctgctgga gatggcggac gcgatggata tgttctgcca 5520

5461 cgggctctac gcggcgcacg ccgacgacct ctaccgcctg cgctacctat acaagacggt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 agggttggtt tgcgcattca cagttctccg caagaattga ttggctccaa ttcttggagt 5580

5521 tcccaaccaa acgcgtaagt gtcaagaggc gttcttaact aaccgaggtt aagaacctca 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ggtgaatccg ttagcgaggt gccgccggct tccattcagg tcgaggtggc ccggctccat 5640

5581 ccacttaggc aatcgctcca cggcggccga aggtaagtcc agctccaccg ggccgaggta 5640

.......... .......... .......... ....... tet(0-612) ORF 3 .......

.......... .......... .......... .......... .......... ..........

5641 gcaccgcgac gcaacgcggg gaggcagaca aggtataggg cggcgcctac aatccatgcc 5700

5641 cgtggcgctg cgttgcgccc ctccgtctgt tccatatccc gccgcggatg ttaggtacgg 5700

.......... ....... tet(0-612) ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 aacccgttcc atgtgctcgc cgaggcggca taaatcgccg tgacgatcag cggtccagtg 5760

5701 ttgggcaagg tacacgagcg gctccgccgt atttagcggc actgctagtc gccaggtcac 5760

.......... ....... tet(0-612) ORF 3 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 atcgaagtta ggctggtaag agccgcgagc gatccttgaa gctgtccctg atggtcgtca 5820

5761 tagcttcaat ccgaccattc tcggcgctcg ctaggaactt cgacagggac taccagcagt 5820

.......... ....... tet(0-612) ORF 3 ....... .......... ORF 3

.......... .......... .......... .......... .......... ..........

5821 tctacctgcc tggacagcat ggcctgcaac gcgggcatcc cgatgccgcc ggaagcgaga 5880

5821 agatggacgg acctgtcgta ccggacgttg cgcccgtagg gctacggcgg ccttcgctct 5880

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... .......... NruI(5917) .......... ..........

5881 agaatcataa tggggaaggc catccagcct cgcgtcgcga acgccagcaa gacgtagccc 5940

5881 tcttagtatt accccttccg gtaggtcgga gcgcagcgct tgcggtcgtt ctgcatcggg 5940

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 agcgcgtcgg ccgccatgcc ggcgataatg gcctgcttct cgccgaaacg tttggtggcg 6000

5941 tcgcgcagcc ggcggtacgg ccgctattac cggacgaaga gcggctttgc aaaccaccgc 6000

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 ggaccagtga cgaaggcttg agcgagggcg tgcaagattc cgaataccgc aagcgacagg 6060

6001 cctggtcact gcttccgaac tcgctcccgc acgttctaag gcttatggcg ttcgctgtcc 6060

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ccgatcatcg tcgcgctcca gcgaaagcgg tcctcgccga aaatgaccca gagcgctgcc 6120

6061 ggctagtagc agcgcgaggt cgctttcgcc aggagcggct tttactgggt ctcgcgacgg 6120

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ggcacctgtc ctacgagttg catgataaag aagacagtca taagtgcggc gacgatagtc 6180

6121 ccgtggacag gatgctcaac gtactatttc ttctgtcagt attcacgccg ctgctatcag 6180

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 atgccccgcg cccaccggaa ggagctgact gggttgaagg ctctcaaggg catcggtcga 6240

6181 tacggggcgc gggtggcctt cctcgactga cccaacttcc gagagttccc gtagccagct 6240

.......... .... tet(0-612) ORF 3 ORF 3 .... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 gatcccggtg cctaatgagt gagctaactt acattaattg cgttgcgctc actgcccgct 6300

6241 ctagggccac ggattactca ctcgattgaa tgtaattaac gcaacgcgag tgacgggcga 6300

.......... .......... ORF 3 .......... .......... ORF 1 lacI

.......... .......... .......... .......... .......... ..........

6301 ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg cgcggggaga 6360

6301 aaggtcagcc ctttggacag cacggtcgac gtaattactt agccggttgc gcgcccctct 6360

.......... ....... ORF 3 ORF 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ggcggtttgc gtattgggcg ccagggtggt ttttcttttc accagtgaga cgggcaacag 6420

6361 ccgccaaacg cataacccgc ggtcccacca aaaagaaaag tggtcactct gcccgttgtc 6420

.......... ....... ORF 3 ORF 1 lacI ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 ctgattgccc ttcaccgcct ggccctgaga gagttgcagc aagcggtcca cgctggtttg 6480

6421 gactaacggg aagtggcgga ccgggactct ctcaacgtcg ttcgccaggt gcgaccaaac 6480

.......... ....... ORF 3 ORF 1 lacI ....... .......... ..........

.......... .......... .......... HpaI(6514) .......... ..........

6481 ccccagcagg cgaaaatcct gtttgatggt ggttaacggc gggatataac atgagctgtc 6540

6481 ggggtcgtcc gcttttagga caaactacca ccaattgccg ccctatattg tactcgacag 6540

.......... ORF 3 ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ttcggtatcg tcgtatccca ctaccgagat atccgcacca acgcgcagcc cggactcggt 6600

6541 aagccatagc agcatagggt gatggctcta taggcgtggt tgcgcgtcgg gcctgagcca 6600

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 aatggcgcgc attgcgccca gcgccatctg atcgttggca accagcatcg cagtgggaac 6660

6601 ttaccgcgcg taacgcgggt cgcggtagac tagcaaccgt tggtcgtagc gtcacccttg 6660

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gatgccctca ttcagcattt gcatggtttg ttgaaaaccg gacatggcac tccagtcgcc 6720

6661 ctacgggagt aagtcgtaaa cgtaccaaac aacttttggc ctgtaccgtg aggtcagcgg 6720

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 ttcccgttcc gctatcggct gaatttgatt gcgagtgaga tatttatgcc agccagccag 6780

6721 aagggcaagg cgatagccga cttaaactaa cgctcactct ataaatacgg tcggtcggtc 6780

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... ApaI(6813) .......... ..........

6781 acgcagacgc gccgagacag aacttaatgg gcccgctaac agcgcgattt gctggtgacc 6840

6781 tgcgtctgcg cggctctgtc ttgaattacc cgggcgattg tcgcgctaaa cgaccactgg 6840

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 caatgcgacc agatgctcca cgcccagtcg cgtaccgtct tcatgggaga aaataatact 6900

6841 gttacgctgg tctacgaggt gcgggtcagc gcatggcaga agtaccctct tttattatga 6900

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 gttgatgggt gtctggtcag agacatcaag aaataacgcc ggaacattag tgcaggcagc 6960

6901 caactaccca cagaccagtc tctgtagttc tttattgcgg ccttgtaatc acgtccgtcg 6960

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 ttccacagca atggcatcct ggtcatccag cggatagtta atgatcagcc cactgacgcg 7020

6961 aaggtgtcgt taccgtagga ccagtaggtc gcctatcaat tactagtcgg gtgactgcgc 7020

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 ttgcgcgaga agattgtgca ccgccgcttt acaggcttcg acgccgcttc gttctaccat 7080

7021 aacgcgctct tctaacacgt ggcggcgaaa tgtccgaagc tgcggcgaag caagatggta 7080

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 cgacaccacc acgctggcac ccagttgatc ggcgcgagat ttaatcgccg cgacaatttg 7140

7081 gctgtggtgg tgcgaccgtg ggtcaactag ccgcgctcta aattagcggc gctgttaaac 7140

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 cgacggcgcg tgcagggcca gactggaggt ggcaacgcca atcagcaacg actgtttgcc 7200

7141 gctgccgcgc acgtcccggt ctgacctcca ccgttgcggt tagtcgttgc tgacaaacgg 7200

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 cgccagttgt tgtgccacgc ggttgggaat gtaattcagc tccgccatcg ccgcttccac 7260

7201 gcggtcaaca acacggtgcg ccaaccctta cattaagtcg aggcggtagc ggcgaaggtg 7260

.......... .......... ORF 1 lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tttttcccgc gttttcgcag aaacgtggct ggcctggttc accacgcggg aaacggtctg 7320

7261 aaaaagggcg caaaagcgtc tttgcaccga ccggaccaag tggtgcgccc tttgccagac 7320

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 ataagagaca ccggcatact ctgcgacatc gtataacgtt actggtttca cattcaccac 7380

7321 tattctctgt ggccgtatga gacgctgtag catattgcaa tgaccaaagt gtaagtggtg 7380

.......... .......... lacI .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 cctgaattga ctctcttccg ggcgctatca tgccataccg cgaaaggttt tgcgccattc 7440

7381 ggacttaact gagagaaggc ccgcgatagt acggtatggc gctttccaaa acgcggtaag 7440

.......... .......... .......... .......... .......... ..........