Sequence of pEXP1-DEST_(Expressway_in

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Sequence of pEXP1-DEST_(Expressway_in_vitro)

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Sequence of pEXP1-DEST (Expressway in vitro) : 4622 BP; 1205 A; 1061 C; 1128 G; 1228 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct 60

1 ctagagctag ggcgctttaa ttatgctgag tgatatccct ctggtgttgc caaagggaga 60

.......... .......... T7_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat 120

61 tctttattaa aacaaattga aattcttcct ctatatgtat acgccccaag agtagtagta 120

.......... ....... T7_transl_en_RBS ....... .......... 6xHis

.......... NheI(136) .......... .......... .......... ..........

121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac 180

121 gtagtagtac cataccgatc gtactgacca cctgtcgttt acccagccct agacatgctg 180

6xHis Xpress_fwd_pri T7_gene10_leader T7_leader Xpress_EK

.......... .......... .......... .......... .......... ..........

181 gatgacgata aggatcatca aacaagtttg tacaaaaaag ctgaacgaga aacgtaaaat 240

181 ctactgctat tcctagtagt ttgttcaaac atgttttttc gacttgctct ttgcatttta 240

Xpress_EK .......... .......... .......... attR1 attR2

.......... .......... .......... .......... .......... ..........

241 gatataaata tcaatatatt aaattagatt ttgcataaaa aacagactac ataatactgt 300

241 ctatatttat agttatataa tttaatctaa aacgtatttt ttgtctgatg tattatgaca 300

.......... ......... attR1 attR2 ......... .......... ..........

.......... ..... NotI(328),EagI(328) ..... .......... ..........

301 aaaacacaac atatccagtc actatggcgg ccgcattagg caccccaggc tttacacttt 360

301 ttttgtgttg tataggtcag tgataccgcc ggcgtaatcc gtggggtccg aaatgtgaaa 360

......... attR1 attR2 ......... .......... .......... lac_pro

.......... .......... .......... .......... .......... ..........

361 atgcttccgg ctcgtataat gtgtggattt tgagttagga tccggcgaga ttttcaggag 420

361 tacgaaggcc gagcatatta cacacctaaa actcaatcct aggccgctct aaaagtcctc 420

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctaaggaagc taaaatggag aaaaaaatca ctggatatac caccgttgat atatcccaat 480

421 gattccttcg attttacctc tttttttagt gacctatatg gtggcaacta tatagggtta 480

.......... .......... ........ ORF 3 CAT/CamR ........ ..........

.......... .......... .......... .......... .......... ..........

481 ggcatcgtaa agaacatttt gaggcatttc agtcagttgc tcaatgtacc tataaccaga 540

481 ccgtagcatt tcttgtaaaa ctccgtaaag tcagtcaacg agttacatgg atattggtct 540

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

541 ccgttcagct ggatattacg gcctttttaa agaccgtaaa gaaaaataag cacaagtttt 600

541 ggcaagtcga cctataatgc cggaaaaatt tctggcattt ctttttattc gtgttcaaaa 600

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... EcoRI(648) ..........

601 atccggcctt tattcacatt cttgcccgcc tgatgaatgc tcatccggaa ttccgtatgg 660

601 taggccggaa ataagtgtaa gaacgggcgg actacttacg agtaggcctt aaggcatacc 660

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 caatgaaaga cggtgagctg gtgatatggg atagtgttca cccttgttac accgttttcc 720

661 gttactttct gccactcgac cactataccc tatcacaagt gggaacaatg tggcaaaagg 720

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 atgagcaaac tgaaacgttt tcatcgctct ggagtgaata ccacgacgat ttccggcagt 780

721 tactcgtttg actttgcaaa agtagcgaga cctcacttat ggtgctgcta aaggccgtca 780

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 ttctacacat atattcgcaa gatgtggcgt gttacggtga aaacctggcc tatttcccta 840

781 aagatgtgta tataagcgtt ctacaccgca caatgccact tttggaccgg ataaagggat 840

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 aagggtttat tgagaatatg tttttcgtct cagccaatcc ctgggtgagt ttcaccagtt 900

841 ttcccaaata actcttatac aaaaagcaga gtcggttagg gacccactca aagtggtcaa 900

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... NcoI(949) ..........

901 ttgatttaaa cgtggccaat atggacaact tcttcgcccc cgttttcacc atgggcaaat 960

901 aactaaattt gcaccggtta tacctgttga agaagcgggg gcaaaagtgg tacccgttta 960

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 attatacgca aggcgacaag gtgctgatgc cgctggcgat tcaggttcat catgccgtct 1020

961 taatatgcgt tccgctgttc cacgactacg gcgaccgcta agtccaagta gtacggcaga 1020

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 gtgatggctt ccatgtcggc agaatgctta atgaattaca acagtactgc gatgagtggc 1080

1021 cactaccgaa ggtacagccg tcttacgaat tacttaatgt tgtcatgacg ctactcaccg 1080

.......... ........ ORF 3 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 agggcggggc gtaatctaga ggatccggct tactaaaagc cagataacag tatgcgtatt 1140

1081 tcccgccccg cattagatct cctaggccga atgattttcg gtctattgtc atacgcataa 1140

ORF 3 CAT/CamR ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tgcgcgctga tttttgcggt ataagaatat atactgatat gtatacccga agtatgtcaa 1200

1141 acgcgcgact aaaaacgcca tattcttata tatgactata catatgggct tcatacagtt 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aaagaggtgt gctatgaagc agcgtattac agtgacagtt gacagcgaca gctatcagtt 1260

1201 tttctccaca cgatacttcg tcgcataatg tcactgtcaa ctgtcgctgt cgatagtcaa 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gctcaaggca tatatgatgt caatatctcc ggtctggtaa gcacaaccat gcagaatgaa 1320

1261 cgagttccgt atatactaca gttatagagg ccagaccatt cgtgttggta cgtcttactt 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gcccgtcgtc tgcgtgccga acgctggaaa gcggaaaatc aggaagggat ggctgaggtc 1380

1321 cgggcagcag acgcacggct tgcgaccttt cgccttttag tccttcccta ccgactccag 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gcccggttta ttgaaatgaa cggctctttt gctgacgaga acagggactg gtgaaatgca 1440

1381 cgggccaaat aactttactt gccgagaaaa cgactgctct tgtccctgac cactttacgt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gtttaaggtt tacacctata aaagagagag ccgttatcgt ctgtttgtgg atgtacagag 1500

1441 caaattccaa atgtggatat tttctctctc ggcaatagca gacaaacacc tacatgtctc 1500

.......... .......... ccdB .......... .......... ..........

.......... SmaI(1519) .......... .......... .......... ..........

1501 tgatattatt gacacgcccg ggcgacggat ggtgatcccc ctggccagtg cacgtctgct 1560

1501 actataataa ctgtgcgggc ccgctgccta ccactagggg gaccggtcac gtgcagacga 1560

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gtcagataaa gtctcccgtg aactttaccc ggtggtgcat atcggggatg aaagctggcg 1620

1561 cagtctattt cagagggcac ttgaaatggg ccaccacgta tagcccctac tttcgaccgc 1620

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 catgatgacc accgatatgg ccagtgtgcc ggtctccgtt atcggggaag aagtggctga 1680

1621 gtactactgg tggctatacc ggtcacacgg ccagaggcaa tagccccttc ttcaccgact 1680

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata 1740

1681 agagtcggtg gcgcttttac tgtagttttt gcggtaattg gactacaaga ccccttatat 1740

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aatgtcaggc tcccttatac acagccagtc tgcaggtcga ccatagtgac tggatatgtt 1800

1741 ttacagtccg agggaatatg tgtcggtcag acgtccagct ggtatcactg acctatacaa 1800

.......... .......... .......... .......... ......... attR2 attR1

.......... .......... .......... .......... .......... ..........

1801 gtgttttaca gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat 1860

1801 cacaaaatgt cataatacat cagacaaaaa atacgtttta gattaaatta tataactata 1860

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... .......... ........ HindIII(1914)

1861 ttatatcatt ttacgtttct cgttcagctt tcttgtacaa agtggttcga tcgaagcttg 1920

1861 aatatagtaa aatgcaaaga gcaagtcgaa agaacatgtt tcaccaagct agcttcgaac 1920

......... attR2 attR1 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 atccggctgc taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat 1980

1921 taggccgacg attgtttcgg gctttccttc gactcaaccg acgacggtgg cgactcgtta 1980

.......... .......... .......... T7_term ....... T7_Terminal_pri

.......... .......... .......... .......... .......... ..........

1981 aactagcata accccttggg gcctctaaac gggtcttgag gggttttttg ctgaaaggag 2040

1981 ttgatcgtat tggggaaccc cggagatttg cccagaactc cccaaaaaac gactttcctc 2040

.......... .......... T7_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gaactatatc cggatctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca 2100

2041 cttgatatag gcctagaccg cattatcgct tctccgggcg tggctagcgg gaagggttgt 2100

T7_term .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg 2160

2101 caacgcgtcg gacttaccgc ttaccctgcg cgggacatcg ccgcgtaatt cgcgccgccc 2160

.......... .......... .......... .......... .......... f1_origin

.......... .......... .......... .......... .......... ..........

2161 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt 2220

2161 acaccaccaa tgcgcgtcgc actggcgatg tgaacggtcg cgggatcgcg ggcgaggaaa 2220

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 2280

2221 gcgaaagaag ggaaggaaag agcggtgcaa gcggccgaaa ggggcagttc gagatttagc 2280

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ggggctccct ttagggttcc gatttagagc tttacggcac ctcgaccgca aaaaacttga 2340

2281 ccccgaggga aatcccaagg ctaaatctcg aaatgccgtg gagctggcgt tttttgaact 2340

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 2400

2341 aaacccacta ccaagtgcat cacccggtag cgggactatc tgccaaaaag cgggaaactg 2400

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 2460

2401 caacctcagg tgcaagaaat tatcacctga gaacaaggtt tgaccttgtt gtgagttggg 2460

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tatcgcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa 2520

2461 atagcgccag ataagaaaac taaatattcc ctaaaacggc taaagccgga taaccaattt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 aaatgagctg atttaacaaa tatttaacgc gaattttaac aaaatattaa cgtttacaat 2580

2521 tttactcgac taaattgttt ataaattgcg cttaaaattg ttttataatt gcaaatgtta 2580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatacagg 2640

2581 aagcggacta cgccataaaa gaggaatgcg tagacacgcc ataaagtgtg gcgtatgtcc 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc 2700

2641 accgtgaaaa gcccctttac acgcgccttg gggataaaca aataaaaaga tttatgtaag 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag 2760

2701 tttatacata ggcgagtact ctgttattgg gactatttac gaagttatta taactttttc 2760

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg 2820

2761 cttctcatac tcataagttg taaaggcaca gcgggaataa gggaaaaaac gccgtaaaac 2820

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

2821 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt 2880

2821 ggaaggacaa aaacgagtgg gtctttgcga ccactttcat tttctacgac ttctagtcaa 2880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt 2940

2881 cccacgtgct cacccaatgt agcttgacct agagttgtcg ccattctagg aactctcaaa 2940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt 3000

2941 agcggggctt cttgcaaaag gttactactc gtgaaaattt caagacgata caccgcgcca 3000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa 3060

3001 taatagggca taactgcggc ccgttctcgt tgagccagcg gcgtatgtga taagagtctt 3060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag 3120

3061 actgaaccaa ctcatgagtg gtcagtgtct tttcgtagaa tgcctaccgt actgtcattc 3120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac 3180

3121 tcttaatacg tcacgacggt attggtactc actattgtga cgccggttga atgaagactg 3180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac 3240

3181 ttgctagcct cctggcttcc tcgattggcg aaaaaacgtg ttgtaccccc tagtacattg 3240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agagtgacac 3300

3241 agcggaacta gcaacccttg gcctcgactt acttcggtat ggtttgctgc tctcactgtg 3300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cacgatgcct gtagcaatgc caacaacgtt gcgcaaacta ttaactggcg aactacttac 3360

3301 gtgctacgga catcgttacg gttgttgcaa cgcgtttgat aattgaccgc ttgatgaatg 3360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact 3420

3361 agatcgaagg gccgttgtta attatctgac ctacctccgc ctatttcaac gtcctggtga 3420

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg 3480

3421 agacgcgagc cgggaaggcc gaccgaccaa ataacgacta tttagacctc ggccactcgc 3480

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagcgctccc gtatcgtagt 3540

3481 acccagagcg ccatagtaac gtcgtgaccc cggtctacca ttcgcgaggg catagcatca 3540

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat 3600

3541 atagatgtgc tgcccctcag tccgttgata cctacttgct ttatctgtct agcgactcta 3600

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta 3660

3601 tccacggagt gactaattcg taaccattga cagtctggtt caaatgagta tatatgaaat 3660

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa 3720

3661 ctaactaaat tttgaagtaa aaattaaatt ttcctagatc cacttctagg aaaaactatt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga 3780

3721 agagtactgg ttttagggaa ttgcactcaa aagcaaggtg actcgcagtc tggggcatct 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac 3840

3781 tttctagttt cctagaagaa ctctaggaaa aaaagacgcg cattagacga cgaacgtttg 3840

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3841 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt 3900

3841 tttttttggt ggcgatggtc gccaccaaac aaacggccta gttctcgatg gttgagaaaa 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc 3960

3901 aggcttccat tgaccgaagt cgtctcgcgt ctatggttta tgacaggaag atcacatcgg 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat 4020

3961 catcaatccg gtggtgaagt tcttgagaca tcgtggcgga tgtatggagc gagacgatta 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag 4080

4021 ggacaatggt caccgacgac ggtcaccgct attcagcaca gaatggccca acctgagttc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc 4140

4081 tgctatcaat ggcctattcc gcgtcgccag cccgacttgc cccccaagca cgtgtgtcgg 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag 4200

4141 gtcgaacctc gcttgctgga tgtggcttga ctctatggat gtcgcactcg atactctttc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac 4260

4201 gcggtgcgaa gggcttccct ctttccgcct gtccataggc cattcgccgt cccagccttg 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg 4320

4261 tcctctcgcg tgctccctcg aaggtccccc tttgcggacc atagaaatat caggacagcc 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct 4380

4321 caaagcggtg gagactgaac tcgcagctaa aaacactacg agcagtcccc ccgcctcgga 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 atggaaaaac gccagcaacg cggccttttt acggttcctg ggcttttgct ggccttttgc 4440

4381 tacctttttg cggtcgttgc gccggaaaaa tgccaaggac ccgaaaacga ccggaaaacg 4440

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga 4500

4441 agtgtacaag aaaggacgca ataggggact aagacaccta ttggcataat ggcggaaact 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga 4560

4501 cactcgacta tggcgagcgg cgtcggcttg ctggctcgcg tcgctcagtc actcgctcct 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg 4620

4561 tcgccttctc gcgggttatg cgtttggcgg agaggggcgc gcaaccggct aagtaattac 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 ca........ .......... .......... .......... .......... .......... 4622

4621 gt........ .......... .......... .......... .......... .......... 4622

.......... .......... .......... .......... .......... ..........