ff LOCUS pEXP2-DEST (Expressway in vitro) 4403 bp DNA SYN 18-Sep-2007 DEFINITION pEXP2-DEST (Expressway in vitro) ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4403 /organism="pEXP2-DEST (Expressway in vitro)" /mol_type="other DNA" promoter 20..38 /label="T7_promoter" misc_feature 98..222 /label="attR1" misc_feature complement(122..222) /label="attR2" promoter 247..270 /label="lac_promoter" CDS 331..990 /label="CAT/CamR" /gene="CAT/CamR" /note="ORF frame 1" /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAF LKTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETF SSLWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNV ANMDNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGG A*" gene 331..990 /label="CAT/CamR" /gene="CAT/CamR" misc_feature 1332..1637 /label="ccdB" misc_feature 1678..1778 /label="attR2" misc_feature complement(1678..1785) /label="attR1" misc_feature 1822..1863 /label="V5" misc_feature 1873..1890 /label="6xHis" terminator 1945..1980 /label="T7_terminator" promoter 2088..2116 /label="AmpR_promoter" gene 2067..2530 /label="zeo" /gene="zeo" gene 2156..2527 /label="bleo" /gene="bleo" misc_feature 2156..2530 /label="sh_ble" CDS 2551..3411 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 1" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDESDTTMPVAMPTTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 2551..3411 /label="Ampicillin" /gene="Ampicillin" rep_origin 3566..4182 /label="pBR322_origin" ORIGIN 1 GATCTCGATC CCGCGAAATT AATACGACTC ACTATAGGGA GACCACAACG GTTTCCCTCT 61 AGAAATAATT TTGTTTAACT TTAAGAAGGA ATTATCAACA AGTTTGTACA AAAAAGCTGA 121 ACGAGAAACG TAAAATGATA TAAATATCAA TATATTAAAT TAGATTTTGC ATAAAAAACA 181 GACTACATAA TACTGTAAAA CACAACATAT CCAGTCACTA TGGCGGCCGC ATTAGGCACC 241 CCAGGCTTTA CACTTTATGC TTCCGGCTCG TATAATGTGT GGATTTTGAG TTAGGATCCG 301 GCGAGATTTT CAGGAGCTAA GGAAGCTAAA ATGGAGAAAA AAATCACTGG ATATACCACC 361 GTTGATATAT CCCAATGGCA TCGTAAAGAA CATTTTGAGG CATTTCAGTC AGTTGCTCAA 421 TGTACCTATA ACCAGACCGT TCAGCTGGAT ATTACGGCCT TTTTAAAGAC CGTAAAGAAA 481 AATAAGCACA AGTTTTATCC GGCCTTTATT CACATTCTTG CCCGCCTGAT GAATGCTCAT 541 CCGGAATTCC GTATGGCAAT GAAAGACGGT GAGCTGGTGA TATGGGATAG TGTTCACCCT 601 TGTTACACCG TTTTCCATGA GCAAACTGAA ACGTTTTCAT CGCTCTGGAG TGAATACCAC 661 GACGATTTCC GGCAGTTTCT ACACATATAT TCGCAAGATG TGGCGTGTTA CGGTGAAAAC 721 CTGGCCTATT TCCCTAAAGG GTTTATTGAG AATATGTTTT TCGTCTCAGC CAATCCCTGG 781 GTGAGTTTCA CCAGTTTTGA TTTAAACGTG GCCAATATGG ACAACTTCTT CGCCCCCGTT 841 TTCACCATGG GCAAATATTA TACGCAAGGC GACAAGGTGC TGATGCCGCT GGCGATTCAG 901 GTTCATCATG CCGTCTGTGA TGGCTTCCAT GTCGGCAGAA TGCTTAATGA ATTACAACAG 961 TACTGCGATG AGTGGCAGGG CGGGGCGTAA AGATCTGGAT CCGGCTTACT AAAAGCCAGA 1021 TAACAGTATG CGTATTTGCG CGCTGATTTT TGCGGTATAA GAATATATAC TGATATGTAT 1081 ACCCGAAGTA TGTCAAAAAG AGGTGTGCTA TGAAGCAGCG TATTACAGTG ACAGTTGACA 1141 GCGACAGCTA TCAGTTGCTC AAGGCATATA TGATGTCAAT ATCTCCGGTC TGGTAAGCAC 1201 AACCATGCAG AATGAAGCCC GTCGTCTGCG TGCCGAACGC TGGAAAGCGG AAAATCAGGA 1261 AGGGATGGCT GAGGTCGCCC GGTTTATTGA AATGAACGGC TCTTTTGCTG ACGAGAACAG 1321 GGACTGGTGA AATGCAGTTT AAGGTTTACA CCTATAAAAG AGAGAGCCGT TATCGTCTGT 1381 TTGTGGATGT ACAGAGTGAT ATTATTGACA CGCCCGGGCG ACGGATGGTG ATCCCCCTGG 1441 CCAGTGCACG TCTGCTGTCA GATAAAGTCT CCCGTGAACT TTACCCGGTG GTGCATATCG 1501 GGGATGAAAG CTGGCGCATG ATGACCACCG ATATGGCCAG TGTGCCGGTC TCCGTTATCG 1561 GGGAAGAAGT GGCTGATCTC AGCCACCGCG AAAATGACAT CAAAAACGCC ATTAACCTGA 1621 TGTTCTGGGG AATATAAATG TCAGGCTCCC TTATACACAG CCAGTCTGCA GGTCGACCAT 1681 AGTGACTGGA TATGTTGTGT TTTACAGTAT TATGTAGTCT GTTTTTTATG CAAAATCTAA 1741 TTTAATATAT TGATATTTAT ATCATTTTAC GTTTCTCGTT CAGCTTTCTT GTACAAAGTG 1801 GTTGATAATT CGAAGCTTGA AGGTAAGCCT ATCCCTAACC CTCTCCTCGG TCTCGATTCT 1861 ACGCGTACCG GTCATCATCA CCATCACCAT TGAGTTTAAA CTATATAGAA TAAAAGAAGA 1921 AACCTTAGCT GAGCAATAAC TAGCATAACC CCTTGGGGCC TCTAAACGGG TCTTGAGGGG 1981 TTTTTTGCTG AAAGGAGGAA CTATATCCGG ATTAACGCTT ACAATTTAGG TGGCACTTTT 2041 CGGGGAAATG TGCGCGGAAC CCCTATTTGT TTATTTTTCT AAATACATTC AAATATGTAT 2101 CCGCTCATGA GACAATAACC CTGATAAATG CTTCAATAAT GTGAGGAGGG CCACCATGGC 2161 CAAGTTGACC AGTGCCGTTC CGGTGCTCAC CGCGCGCGAC GTCGCCGGAG CGGTCGAGTT 2221 CTGGACCGAC CGGCTCGGGT TCTCCCGGGA CTTCGTGGAG GACGACTTCG CCGGTGTGGT 2281 CCGGGACGAC GTGACCCTGT TCATCAGCGC GGTCCAGGAC CAGGTGGTGC CGGACAACAC 2341 CCTGGCCTGG GTGTGGGTGC GCGGCCTGGA CGAGCTGTAC GCCGAGTGGT CGGAGGTCGT 2401 GTCCACGAAC TTCCGGGACG CCTCCGGGCC GGCCATGACC GAGATCGGCG AGCAGCCGTG 2461 GGGGCGGGAG TTCGCCCTGC GCGACCCGGC CGGCAACTGC GTGCACTTCG TGGCCGAGGA 2521 GCAGGACTGA CACATTGAAA AAGGAAGAGT ATGAGTATTC AACATTTCCG TGTCGCCCTT 2581 ATTCCCTTTT TTGCGGCATT TTGCCTTCCT GTTTTTGCTC ACCCAGAAAC GCTGGTGAAA 2641 GTAAAAGATG CTGAAGATCA GTTGGGTGCA CGAGTGGGTT ACATCGAACT GGATCTCAAC 2701 AGCGGTAAGA TCCTTGAGAG TTTTCGCCCC GAAGAACGTT TTCCAATGAT GAGCACTTTT 2761 AAAGTTCTGC TATGTGGCGC GGTATTATCC CGTATTGACG CCGGGCAAGA GCAACTCGGT 2821 CGCCGCATAC ACTATTCTCA GAATGACTTG GTTGAGTACT CACCAGTCAC AGAAAAGCAT 2881 CTTACGGATG GCATGACAGT AAGAGAATTA TGCAGTGCTG CCATAACCAT GAGTGATAAC 2941 ACTGCGGCCA ACTTACTTCT GACAACGATC GGAGGACCGA AGGAGCTAAC CGCTTTTTTG 3001 CACAACATGG GGGATCATGT AACTCGCCTT GATCGTTGGG AACCGGAGCT GAATGAAGCC 3061 ATACCAAACG ACGAGAGTGA CACCACGATG CCTGTAGCAA TGCCAACAAC GTTGCGCAAA 3121 CTATTAACTG GCGAACTACT TACTCTAGCT TCCCGGCAAC AATTAATAGA CTGGATGGAG 3181 GCGGATAAAG TTGCAGGACC ACTTCTGCGC TCGGCCCTTC CGGCTGGCTG GTTTATTGCT 3241 GATAAATCTG GAGCCGGTGA GCGTGGGTCT CGCGGTATCA TTGCAGCACT GGGGCCAGAT 3301 GGTAAGCCCT CCCGTATCGT AGTTATCTAC ACGACGGGGA GTCAGGCAAC TATGGATGAA 3361 CGAAATAGAC AGATCGCTGA GATAGGTGCC TCACTGATTA AGCATTGGTA ACTGTCAGAC 3421 CAAGTTTACT CATATATACT TTAGATTGAT TTAAAACTTC ATTTTTAATT TAAAAGGATC 3481 TAGGTGAAGA TCCTTTTTGA TAATCTCATG ACCAAAATCC CTTAACGTGA GTTTTCGTTC 3541 CACTGAGCGT CAGACCCCGT AGAAAAGATC AAAGGATCTT CTTGAGATCC TTTTTTTCTG 3601 CGCGTAATCT GCTGCTTGCA AACAAAAAAA CGCGCTACCA GCGGTGGTTT GTTTGCCGGA 3661 TCAAGAGCTA CCAACTCTTT TTCCGAAGGT AACTGGCTTC AGCAGAGCGC AGATACCAAA 3721 TACTGTTCTT CTAGTGTAGC CGTAGTTAGG CCACCACTTC AAGAACTCTG TAGCACCGCC 3781 TACATACCTC GCTCTGCTAA TCCTGTTACC AGTGGCTGCT GCCAGTGGCG ATAAGTCGTG 3841 TCTTACCGGG TTGGACTCAA GACGATAGTT ACCGGATAAG GCGCAGCGGT CGGGCTGAAC 3901 GGGGGGTTCG TGCACACAGC CCAGCTTGGA GCGAACGACC TACACCGAAC TGAGATACCT 3961 ACAGCGTGAG CTATGAGAAA GCGCCACGCT TCCCGAAGGG AGAAAGGCGG ACAGGTATCC 4021 GGTAAGCGGC AGGGTCGGAA CAGGAGACGC ACGAGGGAGC TTCCAGGGGG AAACGCCTGG 4081 TATCTTTATA GTCCTGTCGG GTTTCGCCAC CTCTGACTTG AGCGTCGATT TTTGTGATGC 4141 TCGTCAGGGG GGCGGAGCCT ATGGAAAAAC GCCAGCAACG CGGCCTTTTT ACGGTTCCTG 4201 GCCTTTTGCT GGCCTTTTGC TCACATGTTC TTTCCTGCGT TATCCCCTGA TTCTGTGGAT 4261 AACCGTATTA CCGCCTTTGA GTGAGCTGAT ACCGCTCGCC GCAGCCGAAC GACCGAGCGC 4321 AGCGAGTCAG TGAGCGAGGA AGCGGAAGAG CGCCCAATAC GCAAACCGCC TCTCCCCGCG 4381 CGTTGGCCGA TTCATTAATG CAG //0"pEXP2-DEST (Expressway in vitro) "Times New RomanfC0&T7_pro"Times New RomanfC0battR1"Times New RomanfCɆ0zattR2"Times New Romanf0lac_pro"Times New RomanfC0KORF 1"Times New RomanfC0KCAT/CamR"Times New RomanfCɆ04eccdB"Times New Romanf0attR2"Times New RomanfC0attR1"Times New RomanfC0GV5"Times New RomanfCɆ0Qb6xHis"Times New Romanf0T7_term"Times New RomanfC0 zeo"Times New RomanfC0(DAmpR_pro"Times New RomanfCɆ0l bleo"Times New Romanf0l sh_ble"Times New Roman*fC0 S ORF 1"Times New RomanfC0 S  Ampicillin"Times New RomanfCɆ0 V pBR322_origin"Times New Roman fXbaI"Times New Roman:0w fNotI"Times New Roman0 . fPvuII(444),EcoRI(544)"Times New Roman0LRWW fBglII"Times New Roman0\ZXQ_ f/PstI(1670),SalI(1672),BstBI(1810),HindIII(1813)"Times New Roman0d%k,q3q3o fAgeI"Times New RomanK *00K5S9[[Yj fAatII"Times New Roman0fpzz fFspI"Times New Roman+ 0 ftest"Times New Roman0 } ftest"Times New RomanA0M4I+- ftest"Times New Roman0cy||