Sequence of pEXP2-DEST_(Expressway_in

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Sequence of pEXP2-DEST_(Expressway_in_vitro)

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Sequence of pEXP2-DEST (Expressway in vitro) : 4403 BP; 1140 A; 1022 C; 1114 G; 1127 T; 0 other;

.......... .......... .......... .......... .......... XbaI(58)

1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct 60

1 ctagagctag ggcgctttaa ttatgctgag tgatatccct ctggtgttgc caaagggaga 60

.......... .......... T7_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 agaaataatt ttgtttaact ttaagaagga attatcaaca agtttgtaca aaaaagctga 120

61 tctttattaa aacaaattga aattcttcct taatagttgt tcaaacatgt tttttcgact 120

.......... .......... .......... .......... attR1 ..........

.......... .......... .......... .......... .......... ..........

121 acgagaaacg taaaatgata taaatatcaa tatattaaat tagattttgc ataaaaaaca 180

121 tgctctttgc attttactat atttatagtt atataattta atctaaaacg tattttttgt 180

.......... .......... attR1 attR2 .......... ..........

.......... .......... .......... .......... NotI(224) ..........

181 gactacataa tactgtaaaa cacaacatat ccagtcacta tggcggccgc attaggcacc 240

181 ctgatgtatt atgacatttt gtgttgtata ggtcagtgat accgccggcg taatccgtgg 240

.......... ......... attR1 attR2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 ccaggcttta cactttatgc ttccggctcg tataatgtgt ggattttgag ttaggatccg 300

241 ggtccgaaat gtgaaatacg aaggccgagc atattacaca cctaaaactc aatcctaggc 300

.......... lac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gcgagatttt caggagctaa ggaagctaaa atggagaaaa aaatcactgg atataccacc 360

301 cgctctaaaa gtcctcgatt ccttcgattt tacctctttt tttagtgacc tatatggtgg 360

.......... .......... .......... ........ ORF 1 CAT/CamR ........

.......... .......... .......... .......... .......... ..........

361 gttgatatat cccaatggca tcgtaaagaa cattttgagg catttcagtc agttgctcaa 420

361 caactatata gggttaccgt agcatttctt gtaaaactcc gtaaagtcag tcaacgagtt 420

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

421 tgtacctata accagaccgt tcagctggat attacggcct ttttaaagac cgtaaagaaa 480

421 acatggatat tggtctggca agtcgaccta taatgccgga aaaatttctg gcatttcttt 480

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

481 aataagcaca agttttatcc ggcctttatt cacattcttg cccgcctgat gaatgctcat 540

481 ttattcgtgt tcaaaatagg ccggaaataa gtgtaagaac gggcggacta cttacgagta 540

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

EcoRI(544) .......... .......... .......... .......... ..........

541 ccggaattcc gtatggcaat gaaagacggt gagctggtga tatgggatag tgttcaccct 600

541 ggccttaagg cataccgtta ctttctgcca ctcgaccact ataccctatc acaagtggga 600

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

601 tgttacaccg ttttccatga gcaaactgaa acgttttcat cgctctggag tgaataccac 660

601 acaatgtggc aaaaggtact cgtttgactt tgcaaaagta gcgagacctc acttatggtg 660

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

661 gacgatttcc ggcagtttct acacatatat tcgcaagatg tggcgtgtta cggtgaaaac 720

661 ctgctaaagg ccgtcaaaga tgtgtatata agcgttctac accgcacaat gccacttttg 720

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctggcctatt tccctaaagg gtttattgag aatatgtttt tcgtctcagc caatccctgg 780

721 gaccggataa agggatttcc caaataactc ttatacaaaa agcagagtcg gttagggacc 780

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

781 gtgagtttca ccagttttga tttaaacgtg gccaatatgg acaacttctt cgcccccgtt 840

781 cactcaaagt ggtcaaaact aaatttgcac cggttatacc tgttgaagaa gcgggggcaa 840

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttcaccatgg gcaaatatta tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag 900

841 aagtggtacc cgtttataat atgcgttccg ctgttccacg actacggcga ccgctaagtc 900

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 gttcatcatg ccgtctgtga tggcttccat gtcggcagaa tgcttaatga attacaacag 960

901 caagtagtac ggcagacact accgaaggta cagccgtctt acgaattact taatgttgtc 960

.......... ........ ORF 1 CAT/CamR ........ .......... ..........

.......... .......... .......... BglII(991) .......... ..........

961 tactgcgatg agtggcaggg cggggcgtaa agatctggat ccggcttact aaaagccaga 1020

961 atgacgctac tcaccgtccc gccccgcatt tctagaccta ggccgaatga ttttcggtct 1020

........ ORF 1 CAT/CamR ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 taacagtatg cgtatttgcg cgctgatttt tgcggtataa gaatatatac tgatatgtat 1080

1021 attgtcatac gcataaacgc gcgactaaaa acgccatatt cttatatatg actatacata 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 acccgaagta tgtcaaaaag aggtgtgcta tgaagcagcg tattacagtg acagttgaca 1140

1081 tgggcttcat acagtttttc tccacacgat acttcgtcgc ataatgtcac tgtcaactgt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gcgacagcta tcagttgctc aaggcatata tgatgtcaat atctccggtc tggtaagcac 1200

1141 cgctgtcgat agtcaacgag ttccgtatat actacagtta tagaggccag accattcgtg 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 aaccatgcag aatgaagccc gtcgtctgcg tgccgaacgc tggaaagcgg aaaatcagga 1260

1201 ttggtacgtc ttacttcggg cagcagacgc acggcttgcg acctttcgcc ttttagtcct 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 agggatggct gaggtcgccc ggtttattga aatgaacggc tcttttgctg acgagaacag 1320

1261 tccctaccga ctccagcggg ccaaataact ttacttgccg agaaaacgac tgctcttgtc 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggactggtga aatgcagttt aaggtttaca cctataaaag agagagccgt tatcgtctgt 1380

1321 cctgaccact ttacgtcaaa ttccaaatgt ggatattttc tctctcggca atagcagaca 1380

.......... .......... .......... ccdB .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ttgtggatgt acagagtgat attattgaca cgcccgggcg acggatggtg atccccctgg 1440

1381 aacacctaca tgtctcacta taataactgt gcgggcccgc tgcctaccac tagggggacc 1440

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 ccagtgcacg tctgctgtca gataaagtct cccgtgaact ttacccggtg gtgcatatcg 1500

1441 ggtcacgtgc agacgacagt ctatttcaga gggcacttga aatgggccac cacgtatagc 1500

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 gggatgaaag ctggcgcatg atgaccaccg atatggccag tgtgccggtc tccgttatcg 1560

1501 ccctactttc gaccgcgtac tactggtggc tataccggtc acacggccag aggcaatagc 1560

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gggaagaagt ggctgatctc agccaccgcg aaaatgacat caaaaacgcc attaacctga 1620

1561 cccttcttca ccgactagag tcggtggcgc ttttactgta gtttttgcgg taattggact 1620

.......... .......... ccdB .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgttctgggg aatataaatg tcaggctccc ttatacacag ccagtctgca ggtcgaccat 1680

1621 acaagacccc ttatatttac agtccgaggg aatatgtgtc ggtcagacgt ccagctggta 1680

ccdB .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 agtgactgga tatgttgtgt tttacagtat tatgtagtct gttttttatg caaaatctaa 1740

1681 tcactgacct atacaacaca aaatgtcata atacatcaga caaaaaatac gttttagatt 1740

.......... ......... attR2 attR1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 tttaatatat tgatatttat atcattttac gtttctcgtt cagctttctt gtacaaagtg 1800

1741 aaattatata actataaata tagtaaaatg caaagagcaa gtcgaaagaa catgtttcac 1800

.......... attR2 attR1 .......... .......... ..........

.. BstBI(1810),HindIII(1813) .. .......... .......... ..........

1801 gttgataatt cgaagcttga aggtaagcct atccctaacc ctctcctcgg tctcgattct 1860

1801 caactattaa gcttcgaact tccattcgga tagggattgg gagaggagcc agagctaaga 1860

.......... .......... .......... .......... V5 ..........

AgeI(1867) .......... .......... .......... .......... ..........

1861 acgcgtaccg gtcatcatca ccatcaccat tgagtttaaa ctatatagaa taaaagaaga 1920

1861 tgcgcatggc cagtagtagt ggtagtggta actcaaattt gatatatctt attttcttct 1920

.......... .......... 6xHis .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 aaccttagct gagcaataac tagcataacc ccttggggcc tctaaacggg tcttgagggg 1980

1921 ttggaatcga ctcgttattg atcgtattgg ggaaccccgg agatttgccc agaactcccc 1980

.......... .......... .......... .......... T7_term ..........

.......... .......... .......... .......... .......... ..........

1981 ttttttgctg aaaggaggaa ctatatccgg attaacgctt acaatttagg tggcactttt 2040

1981 aaaaaacgac tttcctcctt gatataggcc taattgcgaa tgttaaatcc accgtgaaaa 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat 2100

2041 gcccctttac acgcgccttg gggataaaca aataaaaaga tttatgtaag tttatacata 2100

.......... .......... .......... .......... zeo AmpR_pro

.......... .......... .......... .......... .......... ..........

2101 ccgctcatga gacaataacc ctgataaatg cttcaataat gtgaggaggg ccaccatggc 2160

2101 ggcgagtact ctgttattgg gactatttac gaagttatta cactcctccc ggtggtaccg 2160

AmpR_pro .......... zeo .......... .......... ..........

.......... .......... .......... ......... AatII(2202) .........

2161 caagttgacc agtgccgttc cggtgctcac cgcgcgcgac gtcgccggag cggtcgagtt 2220

2161 gttcaactgg tcacggcaag gccacgagtg gcgcgcgctg cagcggcctc gccagctcaa 2220

.......... ....... zeo bleo sh_ble ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ctggaccgac cggctcgggt tctcccggga cttcgtggag gacgacttcg ccggtgtggt 2280

2221 gacctggctg gccgagccca agagggccct gaagcacctc ctgctgaagc ggccacacca 2280

.......... ....... zeo bleo sh_ble ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ccgggacgac gtgaccctgt tcatcagcgc ggtccaggac caggtggtgc cggacaacac 2340

2281 ggccctgctg cactgggaca agtagtcgcg ccaggtcctg gtccaccacg gcctgttgtg 2340

.......... ....... zeo bleo sh_ble ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cctggcctgg gtgtgggtgc gcggcctgga cgagctgtac gccgagtggt cggaggtcgt 2400

2341 ggaccggacc cacacccacg cgccggacct gctcgacatg cggctcacca gcctccagca 2400

.......... ....... zeo bleo sh_ble ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gtccacgaac ttccgggacg cctccgggcc ggccatgacc gagatcggcg agcagccgtg 2460

2401 caggtgcttg aaggccctgc ggaggcccgg ccggtactgg ctctagccgc tcgtcggcac 2460

.......... ....... zeo bleo sh_ble ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ggggcgggag ttcgccctgc gcgacccggc cggcaactgc gtgcacttcg tggccgagga 2520

2461 ccccgccctc aagcgggacg cgctgggccg gccgttgacg cacgtgaagc accggctcct 2520

.......... ....... zeo bleo sh_ble ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gcaggactga cacattgaaa aaggaagagt atgagtattc aacatttccg t gtcgccctt 2580

2521 cgtcctgact gtgtaacttt ttccttctca tactcataag ttgtaaaggc acagcgggaa 2580

zeo sh_ble .......... .......... ....... ORF 1 Ampicillin .......

.......... .......... .......... .......... .......... ..........

2581 attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa 2640

2581 taagggaaaa aacgccgtaa aacggaagga caaaaacgag tgggtctttg cgaccacttt 2640

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac 2700

2641 cattttctac gacttctagt caacccacgt gctcacccaa tgtagcttga cctagagttg 2700

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt 2760

2701 tcgccattct aggaactctc aaaagcgggg cttcttgcaa aaggttacta ctcgtgaaaa 2760

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt 2820

2761 tttcaagacg atacaccgcg ccataatagg gcataactgc ggcccgttct cgttgagcca 2820

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat 2880

2821 gcggcgtatg tgataagagt cttactgaac caactcatga gtggtcagtg tcttttcgta 2880

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac 2940

2881 gaatgcctac cgtactgtca ttctcttaat acgtcacgac ggtattggta ctcactattg 2940

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg 3000

2941 tgacgccggt tgaatgaaga ctgttgctag cctcctggct tcctcgattg gcgaaaaaac 3000

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc 3060

3001 gtgttgtacc ccctagtaca ttgagcggaa ctagcaaccc ttggcctcga cttacttcgg 3060

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... FspI(3115)

3061 ataccaaacg acgagagtga caccacgatg cctgtagcaa tgccaacaac gttgcgcaaa 3120

3061 tatggtttgc tgctctcact gtggtgctac ggacatcgtt acggttgttg caacgcgttt 3120

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctattaactg gcgaactact tactctagct tcccggcaac aattaataga ctggatggag 3180

3121 gataattgac cgcttgatga atgagatcga agggccgttg ttaattatct gacctacctc 3180

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct 3240

3181 cgcctatttc aacgtcctgg tgaagacgcg agccgggaag gccgaccgac caaataacga 3240

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat 3300

3241 ctatttagac ctcggccact cgcacccaga gcgccatagt aacgtcgtga ccccggtcta 3300

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa 3360

3301 ccattcggga gggcatagca tcaatagatg tgctgcccct cagtccgttg atacctactt 3360

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac 3420

3361 gctttatctg tctagcgact ctatccacgg agtgactaat tcgtaaccat tgacagtctg 3420

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 caagtttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 3480

3421 gttcaaatga gtatatatga aatctaacta aattttgaag taaaaattaa attttcctag 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 3540

3481 atccacttct aggaaaaact attagagtac tggttttagg gaattgcact caaaagcaag 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 3600

3541 gtgactcgca gtctggggca tcttttctag tttcctagaa gaactctagg aaaaaaagac 3600

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3601 cgcgtaatct gctgcttgca aacaaaaaaa cgcgctacca gcggtggttt gtttgccgga 3660

3601 gcgcattaga cgacgaacgt ttgttttttt gcgcgatggt cgccaccaaa caaacggcct 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 3720

3661 agttctcgat ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 3780

3721 atgacaagaa gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 3840

3781 atgtatggag cgagacgatt aggacaatgg tcaccgacga cggtcaccgc tattcagcac 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 3900

3841 agaatggccc aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 3960

3901 ccccccaagc acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg actctatgga 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 4020

3961 tgtcgcactc gatactcttt cgcggtgcga agggcttccc tctttccgcc tgtccatagg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggtaagcggc agggtcggaa caggagacgc acgagggagc ttccaggggg aaacgcctgg 4080

4021 ccattcgccg tcccagcctt gtcctctgcg tgctccctcg aaggtccccc tttgcggacc 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc 4140

4081 atagaaatat caggacagcc caaagcggtg gagactgaac tcgcagctaa aaacactacg 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg 4200

4141 agcagtcccc ccgcctcgga tacctttttg cggtcgttgc gccggaaaaa tgccaaggac 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat 4260

4201 cggaaaacga ccggaaaacg agtgtacaag aaaggacgca ataggggact aagacaccta 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc 4320

4261 ttggcataat ggcggaaact cactcgacta tggcgagcgg cgtcggcttg ctggctcgcg 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg 4380

4321 tcgctcagtc actcgctcct tcgccttctc gcgggttatg cgtttggcgg agaggggcgc 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgttggccga ttcattaatg cag....... .......... .......... .......... 4403

4381 gcaaccggct aagtaattac gtc....... .......... .......... .......... 4403

.......... .......... .......... .......... .......... ..........