Sequence of pEYFP-1

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Sequence of pEYFP-1

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Sequence of pEYFP-1 : 4151 BP; 992 A; 1142 C; 1105 G; 912 T; 0 other;

.......... ..... BglII(27),XhoI(31) SacI(38),HindIII(40) EcoRI(47)

1 tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg 60

1 atcaataatg atcgcgatgg cctgagtcta gagctcgagt tcgaagctta agacgtcagc 60

.......... .......... .......... .......... .......... ..........

KpnI,SacII0),ApaI(75),SmaI(76),BamHI(78) AgeI(84) ....

61 acggtaccgc gggcccggga tccaccggtc gccaccatgg tgagcaaggg cgaggagctg 120

61 tgccatggcg cccgggccct aggtggccag cggtggtacc actcgttccc gctcctcgac 120

.......... .......... .......... ......... d2EYFP ORF 1 .........

.......... .......... .......... .......... .......... ..........

121 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc 180

121 aagtggcccc accacgggta ggaccagctc gacctgccgc tgcatttgcc ggtgttcaag 180

.......... ......... d2EYFP ORF 1 EGFP_N_pri ......... ..........

.......... .......... .......... .......... .......... ..........

181 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc 240

181 tcgcacaggc cgctcccgct cccgctacgg tggatgccgt tcgactggga cttcaagtag 240

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccacctt cggctacggc 300

241 acgtggtggc cgttcgacgg gcacgggacc gggtgggagc actggtggaa gccgatgccg 300

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ctgcagtgct tcgcccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc 360

301 gacgtcacga agcgggcgat ggggctggtg tacttcgtcg tgctgaagaa gttcaggcgg 360

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 atgcccgaag gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag 420

361 tacgggcttc cgatgcaggt cctcgcgtgg tagaagaagt tcctgctgcc gttgatgttc 420

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 acccgcgccg aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc 480

421 tgggcgcggc tccacttcaa gctcccgctg tgggaccact tggcgtagct cgacttcccg 480

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atcgacttca aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc 540

481 tagctgaagt tcctcctgcc gttgtaggac cccgtgttcg acctcatgtt gatgttgtcg 540

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cacaacgtct atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc 600

541 gtgttgcaga tatagtaccg gctgttcgtc ttcttgccgt agttccactt gaagttctag 600

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 cgccacaaca tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc 660

601 gcggtgttgt agctcctgcc gtcgcacgtc gagcggctgg tgatggtcgt cttgtggggg 660

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca gtccgccctg 720

661 tagccgctgc cggggcacga cgacgggctg ttggtgatgg actcgatggt caggcgggac 720

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc 780

721 tcgtttctgg ggttgctctt cgcgctagtg taccaggacg acctcaagca ctggcggcgg 780

.......... ......... d2EYFP ORF 1 EGFP_C_pri ......... ..........

.......... .......... .......... NotI(819) .......... ..........

781 gggatcactc tcggcatgga cgagctgtac aagtaaagcg gccgcgactc tagatcataa 840

781 ccctagtgag agccgtacct gctcgacatg ttcatttcgc cggcgctgag atctagtatt 840

......... d2EYFP ORF 1 ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tcagccatac cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc 900

841 agtcggtatg gtgtaaacat ctccaaaatg aacgaaattt tttggagggt gtggaggggg 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... HpaI(938) .......... ..........

901 tgaacctgaa acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata 960

901 acttggactt tgtattttac ttacgttaac aacaacaatt gaacaaataa cgtcgaatat 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc 1020

961 taccaatgtt tatttcgtta tcgtagtgtt taaagtgttt atttcgtaaa aaaagtgacg 1020

.......... .......... .......... .......... .......... ..........

.......... .......... ......... AflII(1057) ......... ..........

1021 attctagttg tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt 1080

1021 taagatcaac accaaacagg tttgagtagt tacatagaat tccgcattta acattcgcaa 1080

......... EBV_rev_pri ......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 aatattttgt taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag 1140

1081 ttataaaaca attttaagcg caatttaaaa acaatttagt cgagtaaaaa attggttatc 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gccgaaatcg gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt 1200

1141 cggctttagc cgttttaggg aatatttagt tttcttatct ggctctatcc caactcacaa 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gttccagttt ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga 1260

1201 caaggtcaaa ccttgttctc aggtgataat ttcttgcacc tgaggttgca gtttcccgct 1260

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 aaaaccgtct atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg 1320

1261 ttttggcaga tagtcccgct accgggtgat gcacttggta gtgggattag ttcaaaaaac 1320

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gggtcgaggt gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct 1380

1321 cccagctcca cggcatttcg tgatttagcc ttgggatttc cctcgggggc taaatctcga 1380

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgacggggaa agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc 1440

1381 actgcccctt tcggccgctt gcaccgctct ttccttccct tctttcgctt tcctcgcccg 1440

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gctagggcgc tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt 1500

1441 cgatcccgcg accgttcaca tcgccagtgc gacgcgcatt ggtggtgtgg gcggcgcgaa 1500

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aatgcgccgc tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct 1560

1501 ttacgcggcg atgtcccgcg cagtccaccg tgaaaagccc ctttacacgc gccttgggga 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga 1620

1561 taaacaaata aaaagattta tgtaagttta tacataggcg agtactctgt tattgggact 1620

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... .......... ..........

1621 taaatgcttc aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga 1680

1621 atttacgaag ttattataac tttttccttc tcaggactcc gcctttcttg gtcgacacct 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 atgtgtgtca gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa 1740

1681 tacacacagt caatcccaca cctttcaggg gtccgagggg tcgtccgtct tcatacgttt 1740

pBABE_3_pri ........ ...... SV40_enh SV40_pro ...... ..........

.......... .......... .......... .......... .......... ..........

1741 gcatgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca 1800

1741 cgtacgtaga gttaatcagt cgttggtcca cacctttcag gggtccgagg ggtcgtccgt 1800

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 gaagtatgca aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc 1860

1801 cttcatacgt ttcgtacgta gagttaatca gtcgttggta tcagggcggg gattgaggcg 1860

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt 1920

1861 ggtagggcgg ggattgaggc gggtcaaggc gggtaagagg cggggtaccg actgattaaa 1920

......... SV40_enh SV40_pro SV40_origin ......... ..........

.......... .......... .......... .......... .......... ..........

1921 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag 1980

1921 aaaaataaat acgtctccgg ctccggcgga gccggagact cgataaggtc ttcatcactc 1980

SV40_origin SV40pro_F_pri SV40_pro .... .......... ..........

.......... StuI(1996) .......... ClaI(2015) .......... ..........

1981 gaggcttttt tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg 2040

1981 ctccgaaaaa acctccggat ccgaaaacgt ttctagctag ttctctgtcc tactcctagc 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tttcgcatga ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg 2100

2041 aaagcgtact aacttgttct acctaacgtg cgtccaagag gccggcgaac ccacctctcc 2100

.......... .......... ........ ORF 1 Neomycin ........ ..........

.......... .......... .......... .......... .......... ..........

2101 ctattcggct atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg 2160

2101 gataagccga tactgacccg tgttgtctgt tagccgacga gactacggcg gcacaaggcc 2160

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ctgtcagcgc aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat 2220

2161 gacagtcgcg tccccgcggg ccaagaaaaa cagttctggc tggacaggcc acgggactta 2220

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... MscI(2257) .......... FspI(2277)

2221 gaactgcaag acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca 2280

2221 cttgacgttc tgctccgtcg cgccgatagc accgaccggt gctgcccgca aggaacgcgt 2280

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gctgtgctcg acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg 2340

2281 cgacacgagc tgcaacagtg acttcgccct tccctgaccg acgataaccc gcttcacggc 2340

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gggcaggatc tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat 2400

2341 cccgtcctag aggacagtag agtggaacga ggacggctct ttcataggta gtaccgacta 2400

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcaatgcggc ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa 2460

2401 cgttacgccg ccgacgtatg cgaactaggc cgatggacgg gtaagctggt ggttcgcttt 2460

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 catcgcatcg agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg 2520

2461 gtagcgtagc tcgctcgtgc atgagcctac cttcggccag aacagctagt cctactagac 2520

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gacgaagagc atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg 2580

2521 ctgcttctcg tagtccccga gcgcggtcgg cttgacaagc ggtccgagtt ccgctcgtac 2580

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cccgacggcg aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg 2640

2581 gggctgccgc tcctagagca gcactgggta ccgctacgga cgaacggctt atagtaccac 2640

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gaaaatggcc gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat 2700

2641 cttttaccgg cgaaaagacc taagtagctg acaccggccg acccacaccg cctggcgata 2700

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 caggacatag cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac 2760

2701 gtcctgtatc gcaaccgatg ggcactataa cgacttctcg aaccgccgct tacccgactg 2760

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgcttcctcg tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc 2820

2761 gcgaaggagc acgaaatgcc atagcggcga gggctaagcg tcgcgtagcg gaagatagcg 2820

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cttcttgacg agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc 2880

2821 gaagaactgc tcaagaagac tcgccctgag accccaagct ttactggctg gttcgctgcg 2880

Neomycin ORF 1 .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ccaacctgcc atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg 2940

2881 ggttggacgg tagtgctcta aagctaaggt ggcggcggaa gatactttcc aacccgaagc 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt 3000

2941 cttagcaaaa ggccctgcgg ccgacctact aggaggtcgc gcccctagag tacgacctca 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcttcgccca ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa 3060

3001 agaagcgggt gggatccccc tccgattgac tttgtgcctt cctctgttat ggccttcctt 3060

.......... .......... .......... TK_PA_term .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cccgcgctat gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc 3120

3061 gggcgcgata ctgccgttat ttttctgtct tattttgcgt gccacaaccc agcaaacaag 3120

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ataaacgcgg ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt 3180

3121 tatttgcgcc ccaagccagg gtcccgaccg tgagacagct atggggtggc tctggggtaa 3180

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ggggccaata cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag 3240

3181 ccccggttat gcgggcgcaa agaaggaaaa ggggtggggt ggggggttca agcccacttc 3240

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gcccagggct cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat 3300

3241 cgggtcccga gcgtcggttg cagccccgcc gtccgggacg gtatcggagt ccaatgagta 3300

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc 3360

3301 tatatgaaat ctaactaaat tttgaagtaa aaattaaatt ttcctagatc cacttctagg 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag 3420

3361 aaaaactatt agagtactgg ttttagggaa ttgcactcaa aagcaaggtg actcgcagtc 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct 3480

3421 tggggcatct tttctagttt cctagaagaa ctctaggaaa aaaagacgcg cattagacga 3480

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

3481 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac 3540

3481 cgaacgtttg tttttttggt ggcgatggtc gccaccaaac aaacggccta gttctcgatg 3540

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgtccttc 3600

3541 gttgagaaaa aggcttccat tgaccgaagt cgtctcgcgt ctatggttta tgacaggaag 3600

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg 3660

3601 atcacatcgg catcaatccg gtggtgaagt tcttgagaca tcgtggcgga tgtatggagc 3660

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3661 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt 3720

3661 gagacgatta ggacaatggt caccgacgac ggtcaccgct attcagcaca gaatggccca 3720

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... ......... ApaLI(3779)

3721 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt 3780

3721 acctgagttc tgctatcaat ggcctattcc gcgtcgccag cccgacttgc cccccaagca 3780

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc 3840

3781 cgtgtgtcgg gtcgaacctc gcttgctgga tgtggcttga ctctatggat gtcgcactcg 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca 3900

3841 atactctttc gcggtgcgaa gggcttccct ctttccgcct gtccataggc cattcgccgt 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata 3960

3901 cccagccttg tcctctcgcg tgctccctcg aaggtccccc tttgcggacc atagaaatat 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg 4020

3961 caggacagcc caaagcggtg gagactgaac tcgcagctaa aaacactacg agcagtcccc 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct 4080

4021 ccgcctcgga tacctttttg cggtcgttgc gccggaaaaa tgccaaggac cggaaaacga 4080

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta 4140

4081 ccggaaaacg agtgtacaag aaaggacgca ataggggact aagacaccta ttggcataat 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ccgccatgca t......... .......... .......... .......... .......... 4151

4141 ggcggtacgt a......... .......... .......... .......... .......... 4151

.......... .......... .......... .......... .......... ..........