Sequence of pEYFP-N1

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Sequence of pEYFP-N1

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Sequence of pEYFP-N1 : 4733 BP; 1138 A; 1285 C; 1246 G; 1064 T; 0 other;

AseI(7) .......... .......... .......... .......... ..........

1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg 60

1 atcaataatt atcattagtt aatgccccag taatcaagta tcgggtatat acctcaaggc 60

.......... .......... ....... CMV_immearly_pro ....... ..........

.......... .......... .......... .......... .......... ..........

61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc < font color="#000000" style="font-size:10px" face="Courier">cccgcccatt 120

61 gcaatgtatt gaatgccatt taccgggcgg accgactggc gggttgctgg gggcgggtaa 120

.......... ....... CMV_immearly_pro CAG_enh ....... ..........

.......... .......... .......... .......... .......... ..........

121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca 180

121 ctgcagttat tactgcatac aagggtatca ttgcggttat ccctgaaagg taactgcagt 180

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... NdeI(234)

181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc 240

181 tacccacctc ataaatgcca tttgacgggt gaaccgtcat gtagttcaca tagtatacgg 240

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta 300

241 ttcatgcggg ggataactgc agttactgcc atttaccggg cggaccgtaa tacgggtcat 300

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac 360

301 gtactggaat accctgaaag gatgaaccgt catgtagatg cataatcagt agcgataatg 360

.......... ... CAG_enh CMV_immearly_pro ... .......... ..........

.......... .......... .......... .......... .......... ..........

361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg 420

361 gtaccactac gccaaaaccg tcatgtagtt acccgcacct atcgccaaac tgagtgcccc 420

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg 480

421 taaaggttca gaggtggggt aactgcagtt accctcaaac aaaaccgtgg ttttagttgc 480

.......... ....... CMV_immearly_pro ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt 540

481 cctgaaaggt tttacagcat tgttgaggcg gggtaactgc gtttacccgc catccgcaca 540

.......... ....... CMV_immearly_pro .. CMV_fwd_pri CMV_pro .....

.......... .......... .......... .......... .......... ..........

541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta 600

541 tgccaccctc cagatatatt cgtctcgacc aaatcacttg gcagtctagg cgatcgcgat 600

....... CMV_immearly_pro CMV_pro ....... .......... ..........

BglII,XhoI SacI,HindIII(622) EcoRI(629) SalI(639)

601 ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg 660

601 ggcctgagtc tagagctcga gttcgaagct taagacgtca gctgccatgg cgcccgggcc 660

.......... .......... .......... .......... .......... ..........

AgeI(666) .......... .......... .......... .......... ..........

661 gatccaccgg tcgccaccat ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc 720

661 ctaggtggcc agcggtggta ccactcgttc ccgctcctcg acaagtggcc ccaccacggg 720

.......... .......... ......... d2EYFP ORF 1 ......... ..........

.......... .......... .......... .......... .......... ..........

721 atcctggtcg agctggacgg cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggc 780

721 taggaccagc tcgacctgcc gctgcatttg ccggtgttca agtcgcacag gccgctcccg 780

......... EGFP_N_pri d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gagggcgatg ccacctacgg caagctgacc ctgaagttca tctgcaccac cggcaagctg 840

781 ctcccgctac ggtggatgcc gttcgactgg gacttcaagt agacgtggtg gccgttcgac 840

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cccgtgccct ggcccaccct cgtgaccacc ttcggctacg gcctgcagtg cttcgcccgc 900

841 gggcacggga ccgggtggga gcactggtgg aagccgatgc cggacgtcac gaagcgggcg 900

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 taccccgacc acatgaagca gcacgacttc ttcaagtccg ccatgcccga aggctacgtc 960

901 atggggctgg tgtacttcgt cgtgctgaag aagttcaggc ggtacgggct tccgatgcag 960

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 caggagcgca ccatcttctt caaggacgac ggcaactaca agacccgcgc cgaggtgaag 1020

961 gtcctcgcgt ggtagaagaa gttcctgctg ccgttgatgt tctgggcgcg gctccacttc 1020

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttcgagggcg acaccctggt gaaccgcatc gagctgaagg gcatcgactt caaggaggac 1080

1021 aagctcccgc tgtgggacca cttggcgtag ctcgacttcc cgtagctgaa gttcctcctg 1080

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ggcaacatcc tggggcacaa gctggagtac aactacaaca gccacaacgt ctatatcatg 1140

1081 ccgttgtagg accccgtgtt cgacctcatg ttgatgttgt cggtgttgca gatatagtac 1140

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gccgacaagc agaagaacgg catcaaggtg aacttcaaga tccgccacaa catcgaggac 1200

1141 cggctgttcg tcttcttgcc gtagttccac ttgaagttct aggcggtgtt gtagctcctg 1200

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 ggcagcgtgc agctcgccga ccactaccag cagaacaccc ccatcggcga cggccccgtg 1260

1201 ccgtcgcacg tcgagcggct ggtgatggtc gtcttgtggg ggtagccgct gccggggcac 1260

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ctgctgcccg acaaccacta cctgagctac cagtccgccc tgagcaaaga ccccaacgag 1320

1261 gacgacgggc tgttggtgat ggactcgatg gtcaggcggg actcgtttct ggggttgctc 1320

.......... ......... d2EYFP ORF 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aagcgcgatc acatggtcct gctggagttc gtgaccgccg ccgggatcac tctcggcatg 1380

1321 ttcgcgctag tgtaccagga cgacctcaag cactggcggc ggccctagtg agagccgtac 1380

.......... ... d2EYFP ORF 1 EGFP_C_pri ... .......... ..........

.......... .......... NotI(1401) .......... .......... ..........

1381 gacgagctgt acaagtaaag cggccgcgac tctagatcat aatcagccat accacatttg 1440

1381 ctgctcgaca tgttcatttc gccggcgctg agatctagta ttagtcggta tggtgtaaac 1440

ORF 1 .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa 1500

1441 atctccaaaa tgaacgaaat tttttggagg gtgtggaggg ggacttggac tttgtatttt 1500

.......... .......... .......... .......... .......... ..........

.......... HpaI(1520) .......... .......... .......... ..........

1501 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca 1560

1501 acttacgtta acaacaacaa ttgaacaaat aacgtcgaat attaccaatg tttatttcgt 1560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt 1620

1561 tatcgtagtg tttaaagtgt ttatttcgta aaaaaagtga cgtaagatca acaccaaaca 1620

.......... .......... .......... .......... ......... EBV_rev_pri

......... AflII(1639) ......... .......... .......... ..........

1621 ccaaactcat caatgtatct taaggcgtaa attgtaagcg ttaatatttt gttaaaattc 1680

1621 ggtttgagta gttacataga attccgcatt taacattcgc aattataaaa caattttaag 1680

EBV_rev_pri ........ .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcgttaaatt tttgttaaat cagctcattt tttaaccaat aggccgaaat cggcaaaatc 1740

1681 cgcaatttaa aaacaattta gtcgagtaaa aaattggtta tccggcttta gccgttttag 1740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ccttataaat caaaagaata gaccgagata gggttgagtg ttgttccagt ttggaacaag 1800

1741 ggaatattta gttttcttat ctggctctat cccaactcac aacaaggtca aaccttgttc 1800

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

1801 agtccactat taaagaacgt ggactccaac gtcaaagggc gaaaaaccgt ctatcagggc 1860

1801 tcaggtgata atttcttgca cctgaggttg cagtttcccg ctttttggca gatagtcccg 1860

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 gatggcccac tacgtgaacc atcaccctaa tcaagttttt tggggtcgag gtgccgtaaa 1920

1861 ctaccgggtg atgcacttgg tagtgggatt agttcaaaaa accccagctc cacggcattt 1920

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gcactaaatc ggaaccctaa agggagcccc cgatttagag cttgacgggg aaagccggcg 1980

1921 cgtgatttag ccttgggatt tccctcgggg gctaaatctc gaactgcccc tttcggccgc 1980

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 aacgtggcga gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt 2040

1981 ttgcaccgct ctttccttcc cttctttcgc tttcctcgcc cgcgatcccg cgaccgttca 2040

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gtagcggtca cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc 2100

2041 catcgccagt gcgacgcgca ttggtggtgt gggcggcgcg aattacgcgg cgatgtcccg 2100

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gcgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa 2160

2101 cgcagtccac cgtgaaaagc ccctttacac gcgccttggg gataaacaaa taaaaagatt 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat 2220

2161 tatgtaagtt tatacatagg cgagtactct gttattggga ctatttacga agttattata 2220

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tgaaaaagga agagtcctga ggcggaaaga accagctgtg gaatgtgtgt cagttagggt 2280

2221 actttttcct tctcaggact ccgcctttct tggtcgacac cttacacaca gtcaatccca 2280

.......... .......... .......... ......... pBABE_3_pri SV40_enh

.......... .......... .......... .......... .......... ..........

2281 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2340

2281 cacctttcag gggtccgagg ggtcgtccgt cttcatacgt ttcgtacgta gagttaatca 2340

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cagcaaccag gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc 2400

2341 gtcgttggtc cacacctttc aggggtccga ggggtcgtcc gtcttcatac gtttcgtacg 2400

.......... ...... SV40_enh SV40_pro ...... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 atctcaatta gtcagcaacc atagtcccgc ccctaactcc gcccatcccg cccctaactc 2460

2401 tagagttaat cagtcgttgg tatcagggcg gggattgagg cgggtagggc ggggattgag 2460

.......... ...... SV40_enh SV40_pro ...... ......... SV40_origin

.......... .......... .......... .......... .......... ..........

2461 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg 2520

2461 gcgggtcaag gcgggtaaga ggcggggtac cgactgatta aaaaaaataa atacgtctcc 2520

..... SV40_enh SV40_pro SV40_origin ..... ........ SV40pro_F_pri

.......... .......... .......... .......... .......... ..........

2521 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc 2580

2521 ggctccggcg gagccggaga ctcgataagg tcttcatcac tcctccgaaa aaacctccgg 2580

.......... SV40_pro .......... .......... .......... ..........

.......... ClaI(2597) .......... .......... .......... ..........

2581 taggcttttg caaagatcga tcaagagaca ggatgaggat cgtttcgcat gattgaacaa 2640

2581 atccgaaaac gtttctagct agttctctgt cctactccta gcaaagcgta ctaacttgtt 2640

.......... .......... .......... .......... ........ ORF 1 Neomycin

.......... .......... .......... .......... .......... ..........

2641 gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg ctatgactgg 2700

2641 ctacctaacg tgcgtccaag aggccggcga acccacctct ccgataagcc gatactgacc 2700

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc gcaggggcgc 2760

2701 cgtgttgtct gttagccgac gagactacgg cggcacaagg ccgacagtcg cgtccccgcg 2760

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca agacgaggca 2820

2761 ggccaagaaa aacagttctg gctggacagg ccacgggact tacttgacgt tctgctccgt 2820

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... MscI(2839) .......... FspI(2859) .......... ..........

2821 gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct cgacgttgtc 2880

2821 cgcgccgata gcaccgaccg gtgctgcccg caaggaacgc gtcgacacga gctgcaacag 2880

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga tctcctgtca 2940

2881 tgacttcgcc cttccctgac cgacgataac ccgcttcacg gccccgtcct agaggacagt 2940

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg gcggctgcat 3000

2941 agagtggaac gaggacggct ctttcatagg tagtaccgac tacgttacgc cgccgacgta 3000

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat cgagcgagca 3060

3001 tgcgaactag gccgatggac gggtaagctg gtggttcgct ttgtagcgta gctcgctcgt 3060

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga gcatcagggg 3120

3061 gcatgagcct accttcggcc agaacagcta gtcctactag acctgcttct cgtagtcccc 3120

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca tgcccgacgg cgaggatctc 3180

3121 gagcgcggtc ggcttgacaa gcggtccgag ttccgctcgt acgggctgcc gctcctagag 3180

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg ccgcttttct 3240

3181 cagcactggg taccgctacg gacgaacggc ttatagtacc accttttacc ggcgaaaaga 3240

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat agcgttggct 3300

3241 cctaagtagc tgacaccggc cgacccacac cgcctggcga tagtcctgta tcgcaaccga 3300

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct cgtgctttac 3360

3301 tgggcactat aacgacttct cgaaccgccg cttacccgac tggcgaagga gcacgaaatg 3360

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga cgagttcttc 3420

3361 ccatagcggc gagggctaag cgtcgcgtag cggaagatag cggaagaact gctcaagaag 3420

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tgagcgggac tctggggttc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 3480

3421 actcgccctg agaccccaag ctttactggc tggttcgctg cgggttggac ggtagtgctc 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 3540

3481 taaagctaag gtggcggcgg aagatacttt ccaacccgaa gccttagcaa aaggccctgc 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccctaggg 3600

3541 ggccgaccta ctaggaggtc gcgcccctag agtacgacct caagaagcgg gtgggatccc 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ggaggctaac tgaaacacgg aaggagacaa taccggaagg aacccgcgct atgacggcaa 3660

3601 cctccgattg actttgtgcc ttcctctgtt atggccttcc ttgggcgcga tactgccgtt 3660

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 taaaaagaca gaataaaacg cacggtgttg ggtcgtttgt tcataaacgc ggggttcggt 3720

3661 atttttctgt cttattttgc gtgccacaac ccagcaaaca agtatttgcg ccccaagcca 3720

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cccagggctg gcactctgtc gataccccac cgagacccca ttggggccaa tacgcccgcg 3780

3721 gggtcccgac cgtgagacag ctatggggtg gctctggggt aaccccggtt atgcgggcgc 3780

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tttcttcctt ttccccaccc caccccccaa gttcgggtga aggcccaggg ctcgcagcca 3840

3781 aaagaaggaa aaggggtggg gtggggggtt caagcccact tccgggtccc gagcgtcggt 3840

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 acgtcggggc ggcaggccct gccatagcct caggttactc atatatactt tagattgatt 3900

3841 tgcagccccg ccgtccggga cggtatcgga gtccaatgag tatatatgaa atctaactaa 3900

.......... TK_PA_term .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga 3960

3901 attttgaagt aaaaattaaa ttttcctaga tccacttcta ggaaaaacta ttagagtact 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca 4020

3961 ggttttaggg aattgcactc aaaagcaagg tgactcgcag tctggggcat cttttctagt 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac 4080

4021 ttcctagaag aactctagga aaaaaagacg cgcattagac gacgaacgtt tgtttttttg 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg 4140

4081 gtggcgatgg tcgccaccaa acaaacggcc tagttctcga tggttgagaa aaaggcttcc 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag 4200

4141 attgaccgaa gtcgtctcgc gtctatggtt tatgacagga agatcacatc ggcatcaatc 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac 4260

4201 cggtggtgaa gttcttgaga catcgtggcg gatgtatgga gcgagacgat taggacaatg 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt 4320

4261 gtcaccgacg acggtcaccg ctattcagca cagaatggcc caacctgagt tctgctatca 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... ......... ApaLI(4361) .........

4321 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg 4380

4321 atggcctatt ccgcgtcgcc agcccgactt gccccccaag cacgtgtgtc gggtcgaacc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc 4440

4381 tcgcttgctg gatgtggctt gactctatgg atgtcgcact cgatactctt tcgcggtgcg 4440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc 4500

4441 aagggcttcc ctctttccgc ctgtccatag gccattcgcc gtcccagcct tgtcctctcg 4500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc 4560

4501 cgtgctccct cgaaggtccc cctttgcgga ccatagaaat atcaggacag cccaaagcgg 4560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4561 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa 4620

4561 tggagactga actcgcagct aaaaacacta cgagcagtcc ccccgcctcg gatacctttt 4620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4621 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt 4680

4621 tgcggtcgtt gcgccggaaa aatgccaagg accggaaaac gaccggaaaa cgagtgtaca 4680

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccatg cat....... 4733

4681 agaaaggacg caatagggga ctaagacacc tattggcata atggcggtac gta....... 4733

.......... .......... .......... .......... .......... ..........