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pEZ-Frt-lox-DT
Gene/insert name: pEZ-Frt-lox-DT
Insert size (bp): 3100
Species of gene(s): DNA derived from yeast
E. Coli
transposon Tn5
Cosynebacterium
Vector backbone: pBSII KS (Search Vector Database)
Backbone manufacturer: Stratagene
Type of vector: Cre/Lox
Backbone size (bp): 6028
Cloning site 5': Cla I
Site destroyed during cloning: No
Cloning site 3': Don't Know
Site destroyed during cloning: Yes
5' Sequencing primer: T7/M13 (List of Sequencing Primers)
3' Sequencing primer: T7/M13
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Selectable markers: Neomycin,Diphtheriatoxin A
If you did not originally clone this gene, from whom and where did you receive the plasmid used to derive this plasmid: Mark Ansel, CBR Institute for Biomedical Research
Plasmid Provided In: DH5a
Principal Investigator: Klaus Rajewsky
Comments: based on pEasyFlox. Vector for conditional gene targeting