Sequence of pEZ-Frt-lox-DT

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Sequence of pEZ-Frt-lox-DT

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Sequence of pEZ-Frt-lox-DT : 6028 BP; 1445 A; 1587 C; 1542 G; 1454 T; 0 other;

EcoRV(5),ClaI(7) NotI(20) SacII(26) .... .......... ..........

1 tagatatcga tggccatagc ggccgcggat ccataacttc gtataatgta tgctatacga 60

1 atctatagct accggtatcg ccggcgccta ggtattgaag catattacat acgatatgct 60

.......... .......... .......... .......... loxP ..........

.......... .......... .......... .......... .......... ..........

61 agttatcctt tcgagggagc ttcaaaagcg ctctgaagtt cctatacttt ctagagaata 120

61 tcaataggaa agctccctcg aagttttcgc gagacttcaa ggatatgaaa gatctcttat 120

.......... .......... .......... .......... FRT ..........

.......... .......... .......... .......... .......... ..........

121 ggaacttcgg aataggaact tcaagatccc cctggcgaaa gggggatgtg ctgcaaggcg 180

121 ccttgaagcc ttatccttga agttctaggg ggaccgcttt ccccctacac gacgttccgc 180

.......... FRT .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 attaagttgg gtaacgccag ggttttccca gtcacgacgt tgtaaaacga cggccagtga 240

181 taattcaacc cattgcggtc ccaaaagggt cagtgctgca acattttgct gccggtcact 240

.......... .......... .......... M13_pUC_f M13_f20 ..........

.......... NdeI(255) .......... .......... .......... ..........

241 attcgagctc tcccatatgg tcgagcagtg tggttttcaa gaggaagcaa aaagcctctc 300

241 taagctcgag agggtatacc agctcgtcac accaaaagtt ctccttcgtt tttcggagag 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 cacccaggcc tggaatgttt ccacccaatg tcgagcagtg tggttttgca agaggaagca 360

301 gtgggtccgg accttacaaa ggtgggttac agctcgtcac accaaaacgt tctccttcgt 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 aaaagcctct ccacccaggc ctggaatgtt tccacccaat gtcgagcaaa ccccgcccag 420

361 ttttcggaga ggtgggtccg gaccttacaa aggtgggtta cagctcgttt ggggcgggtc 420

.......... .......... .......... .......... .......... ..........

.......... BstBI(440) .......... .......... .......... ..........

421 cgtcttgtca ttggcgaatt cgaacacgca gatgcagtcg gggcggcgcg gtcccaggtc 480

421 gcagaacagt aaccgcttaa gcttgtgcgt ctacgtcagc cccgccgcgc cagggtccag 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cacttcgcat attaaggtga cgcgtgtggc ctcgaacacc gagcgaccct gcagccaata 540

481 gtgaagcgta taattccact gcgcacaccg gagcttgtgg ctcgctggga cgtcggttat 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 tgggatcggc cattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 600

541 accctagccg gtaacttgtt ctacctaacg tgcgtccaag aggccggcga acccacctct 600

.......... .......... NeoR/KanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 660

601 ccgataagcc gatactgacc cgtgttgtct gttagccgac gagactacgg cggcacaagg 660

.......... .......... NeoR/KanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 720

661 ccgacagtcg cgtccccgcg ggccaagaaa aacagttctg gctggacagg ccacgggact 720

.......... .......... NeoR/KanR .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 780

721 tacttgacgt cctgctccgt cgcgccgata gcaccgaccg gtgctgcccg caaggaacgc 780

.......... ....... NeoR/KanR ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

781 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 840

781 gtcgacacga gctgcaacag tgacttcgcc cttccctgac cgacgataac ccgcttcacg 840

.......... ....... NeoR/KanR ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cggggcagga tctcctgtgc atctcacctt gctcctgccg agaaagtatc catcatggct 900

841 gccccgtcct agaggacacg tagagtggaa cgaggacggc tctttcatag gtagtaccga 900

.......... ....... NeoR/KanR ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

901 gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg 960

901 ctacgttacg ccgccgacgt atgcgaacta ggccgatgga cgggtaagct ggtggttcgc 960

.......... ....... NeoR/KanR ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

961 aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat 1020

961 tttgtagcgt agctcgctcg tgcatgagcc taccttcggc cagaacagct agtcctacta 1020

.......... ....... NeoR/KanR ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ctggacgaag agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc 1080

1021 gacctgcttc tcgtagtccc cgagcgcggt cggcttgaca agcggtccga gttccgcgcg 1080

.......... ....... NeoR/KanR ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg 1140

1081 tacgggctgc cgctcctaga gcagcactgg gtaccgctac ggacgaacgg cttatagtac 1140

.......... ....... NeoR/KanR ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc 1200

1141 caccttttac cggcgaaaag acctaagtag ctgacaccgg ccgacccaca ccgcctggcg 1200

.......... ....... NeoR/KanR ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tatcaggaca tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct 1260

1201 atagtcctgt atcgcaaccg atgggcacta taacgacttc tcgaaccgcc gcttacccga 1260

.......... ....... NeoR/KanR ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat 1320

1261 ctggcgaagg agcacgaaat gccatagcgg cgagggctaa gcgtcgcgta gcggaagata 1320

.......... ....... NeoR/KanR ORF 1 ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cgccttcttg acgagttctt ctgaggggat cggcaataaa aagacagaat aaaacgcacg 1380

1321 gcggaagaac tgctcaagaa gactccccta gccgttattt ttctgtctta ttttgcgtgc 1380

....... NeoR/KanR ORF 1 ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ggtgttgggt cgtttgttcg gatccgtcga gggagcttca aaagcgctct gaagttccta 1440

1381 ccacaaccca gcaaacaagc ctaggcagct ccctcgaagt tttcgcgaga cttcaaggat 1440

.......... .......... .......... .......... .......... FRT

.......... .......... .......... .......... .......... ..........

1441 tactttctag agaataggaa cttcggaata ggaacttcaa gatccccctg gcgaaagggg 1500

1441 atgaaagatc tcttatcctt gaagccttat ccttgaagtt ctagggggac cgctttcccc 1500

.......... FRT .......... .......... .......... ..........

SalI(1507) .......... .......... ........ HindIII(1547) ........

1501 gatgtggtcg acataacttc gtataatgta tgctatacga agttataagc ttgcggccgg 1560

1501 ctacaccagc tgtattgaag catattacat acgatatgct tcaatattcg aacgccggcc 1560

.......... .......... loxP .......... .......... ..........

XhoI(1568) .......... .......... .......... .......... ..........

1561 ccgccacctc gagccccagc tggttctttc cgcctcagaa gccatagagc ccaccgcatc 1620

1561 ggcggtggag ctcggggtcg accaagaaag gcggagtctt cggtatctcg ggtggcgtag 1620

.......... .......... .......... .......... ......... bGH_PA_term

.......... .......... .......... .......... .......... ..........

1621 cccagcatgc ctgctattgt cttcccaatc ctcccccttg ctgtcctgcc ccaccccacc 1680

1621 gggtcgtacg gacgataaca gaagggttag gagggggaac gacaggacgg ggtggggtgg 1680

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ccccagaata gaatgacacc tactcagaca atgcgatgca atttcctcat tttattagga 1740

1681 ggggtcttat cttactgtgg atgagtctgt tacgctacgt taaaggagta aaataatcct 1740

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 aaggacagtg ggagtggcac cttccagggt caaggaaggc acgggggagg ggcaaacaac 1800

1741 ttcctgtcac cctcaccgtg gaaggtccca gttccttccg tgccccctcc ccgtttgttg 1800

.......... ......... bGH_PA_term ......... .......... ..........

.......... .......... .......... BclI(1834) .......... ..........

1801 agatggctgg caactagaag gcacagtcga ggctgatcag cgagctctag gatctgcatt 1860

1801 tctaccgacc gttgatcttc cgtgtcagct ccgactagtc gctcgagatc ctagacgtaa 1860

......... bGH_PA_term BGH_r ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccaccactgc tcccattcat cagttccata ggttggaatc taaaatacac aaacaattag 1920

1861 ggtggtgacg agggtaagta gtcaaggtat ccaaccttag attttatgtg tttgttaatc 1920

.......... .......... .......... ........ SV40_3_splice ........

.......... .......... .......... .......... .......... ..........

1921 aatcagtagt ttaacacatt atacacttaa aaattttata tttaccttag agctttaaat 1980

1921 ttagtcatca aattgtgtaa tatgtgaatt tttaaaatat aaatggaatc tcgaaattta 1980

........ SV40_3_splice ........ SV40_int .......... ORF 2

.......... .......... .......... .......... .......... ..........

1981 ctctgtaggt agtttgtcca attatgtcac accacagaag taaggttcct tcacaaagag 2040

1981 gagacatcca tcaaacaggt taatacagtg tggtgtcttc attccaagga agtgtttctc 2040

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 atcgcctgac acgatttcct gcacaggctt gagccatata ctcatacatc gcatcttggc 2100

2041 tagcggactg tgctaaagga cgtgtccgaa ctcggtatat gagtatgtag cgtagaaccg 2100

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 cacgttttcc acgggtttca aaattaatct caagttctac gcttaacgct ttcgcctgtt 2160

2101 gtgcaaaagg tgcccaaagt tttaattaga gttcaagatg cgaattgcga aagcggacaa 2160

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 cccagttatt aatatattca acgctagaac tcccctcagc gaagggaagg ctgagcacta 2220

2161 gggtcaataa ttatataagt tgcgatcttg aggggagtcg cttcccttcc gactcgtgat 2220

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cacgcgaagc accatcaccg aaccttttga taaactcttc cgttccgact tgctccatca 2280

2221 gtgcgcttcg tggtagtggc ttggaaaact atttgagaag gcaaggctga acgaggtagt 2280

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acggttcagt gagacttaaa cctaactctt tcttaatagt ttcggcatta tccactttta 2340

2281 tgccaagtca ctctgaattt ggattgagaa agaattatca aagccgtaat aggtgaaaat 2340

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 gtgcgagaac cttcgtcagt cctggatacg tcactttgac cacgcctcca gcttttccag 2400

2341 cacgctcttg gaagcagtca ggacctatgc agtgaaactg gtgcggaggt cgaaaaggtc 2400

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 agagcgggtt ttcattatct acagagtatc ccgcagcgtc gtatttattg tcggtactat 2460

2401 tctcgcccaa aagtaataga tgtctcatag ggcgtcgcag cataaataac agccatgata 2460

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aaaacccttt ccaatcatcg tcataatttc cttgtgtacc agattttggc ttttgtatac 2520

2461 ttttgggaaa ggttagtagc agtattaaag gaacacatgg tctaaaaccg aaaacatatg 2520

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ctttttgaat ggaatctaca taaccaggtt tagtcccgtg gtacgaagaa aagttttcca 2580

2521 gaaaaactta ccttagatgt attggtccaa atcagggcac catgcttctt ttcaaaaggt 2580

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tcacaaaaga tttagaagaa tcaacaacat catcaggatc catggcgagg acctgcaggt 2640

2581 agtgttttct aaatcttctt agttgttgta gtagtcctag gtaccgctcc tggacgtcca 2640

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 cgaaaggccc ggagatgagg aagaggagaa cagcgcggca gacgtgcgct tttgaagcgt 2700

2641 gctttccggg cctctactcc ttctcctctt gtcgcgccgt ctgcacgcga aaacttcgca 2700

.......... .......... ORF 2 .......... .......... mPGK_F

.......... .......... .......... .......... .......... ..........

2701 gcagaatgcc gggcctccgg aggaccttcg ggcgcccgcc ccgcccctga gcccgcccct 2760

2701 cgtcttacgg cccggaggcc tcctggaagc ccgcgggcgg ggcggggact cgggcgggga 2760

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gagcccgccc ccggacccac cccttcccag cctctgagcc cagaaagcga aggagcaaag 2820

2761 ctcgggcggg ggcctgggtg gggaagggtc ggagactcgg gtctttcgct tcctcgtttc 2820

.......... .......... ORF 2 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ctgctattgg ccgctgcccc aaaggcctac ccgcttccat tgctcagcgg tgctgtccat 2880

2821 gacgataacc ggcgacgggg tttccggatg ggcgaaggta acgagtcgcc acgacaggta 2880

.......... ORF 2 .......... .......... .......... ..........

SpeI(2890) .......... .......... .......... .......... ..........

2881 ctgcacgaga ctagtgagac gtgctacttc catttgtcac gtcctgcacg acgcgagctg 2940

2881 gacgtgctct gatcactctg cacgatgaag gtaaacagtg caggacgtgc tgcgctcgac 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cggggcgggg gggaacttcc tgactagggg aggagtagaa ggtggcgcga aggggccacc 3000

2941 gccccgcccc cccttgaagg actgatcccc tcctcatctt ccaccgcgct tccccggtgg 3000

.......... .......... .......... .......... .......... ..........

.......... .......... AgeI(3025) .......... .......... ..........

3001 aaagaacgga gccggttggc gcctaccggt ggatgtggaa tgtgtgcgag gccagaggcc 3060

3001 tttcttgcct cggccaaccg cggatggcca cctacacctt acacacgctc cggtctccgg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 acttgtgtag cgccaagtgc ccagcggggc tgctaaagcg catgctccag actgccttgg 3120

3061 tgaacacatc gcggttcacg ggtcgccccg acgatttcgc gtacgaggtc tgacggaacc 3120

.......... .......... .......... MSCV_r .......... ..........

.......... .......... .......... .......... .......... ..........

3121 gaaaagcgcc tcccctaccc ggtagaattc gatgtggagc tccaattcgc cctatagtga 3180

3121 cttttcgcgg aggggatggg ccatcttaag ctacacctcg aggttaagcg ggatatcact 3180

.......... .......... .......... .......... .......... T7

.......... .......... .......... .......... .......... ..........

3181 gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg 3240

3181 cagcataatg cgcgcgagtg accggcagca aaatgttgca gcactgaccc ttttgggacc 3240

T7 .......... M13_f20 M13_pUC_f lacZ_a ..........

.......... .......... .......... .......... .......... ..........

3241 cgttacccaa cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga 3300

3241 gcaatgggtt gaattagcgg aacgtcgtgt agggggaaag cggtcgaccg cattatcgct 3300

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 agaggcccgc accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc 3360

3301 tctccgggcg tggctagcgg gaagggttgt caacgcgtcg gacttaccgc ttaccctgcg 3360

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac 3420

3361 cgggacatcg ccgcgtaatt cgcgccgccc acaccaccaa tgcgcgtcgc actggcgatg 3420

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

3421 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt 3480

3421 tgaacggtcg cgggatcgcg ggcgaggaaa gcgaaagaag ggaaggaaag agcggtgcaa 3480

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc 3540

3481 gcggccgaaa ggggcagttc gagatttagc ccccgaggga aatcccaagg ctaaatcacg 3540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc 3600

3541 aaatgccgtg gagctggggt tttttgaact aatcccacta ccaagtgcat cacccggtag 3600

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact 3660

3601 cgggactatc tgccaaaaag cgggaaactg caacctcagg tgcaagaaat tatcacctga 3660

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg 3720

3661 gaacaaggtt tgaccttgtt gtgagttggg atagagccag ataagaaaac taaatattcc 3720

f1_origin .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc 3780

3721 ctaaaacggc taaagccgga taaccaattt tttactcgac taaattgttt ttaaattgcg 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gaattttaac aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc 3840

3781 cttaaaattg ttttataatt gcgaatgtta aatccaccgt gaaaagcccc tttacacgcg 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa 3900

3841 ccttggggat aaacaaataa aaagatttat gtaagtttat acataggcga gtactctgtt 3900

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

3901 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc 3960

3901 attgggacta tttacgaagt tattataact ttttccttct catactcata agttgtaaag 3960

.......... .......... .......... .......... ....... ORF 1 Ampicillin

.......... .......... .......... .......... .......... ..........

3961 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa 4020

3961 gcacagcggg aataagggaa aaaacgccgt aaaacggaag gacaaaaacg agtgggtctt 4020

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa 4080

4021 tgcgaccact ttcattttct acgacttcta gtcaacccac gtgctcaccc aatgtagctt 4080

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg 4140

4081 gacctagagt tgtcgccatt ctaggaactc tcaaaagcgg ggcttcttgc aaaaggttac 4140

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa 4200

4141 tactcgtgaa aatttcaaga cgatacaccg cgccataata gggcataact gcggcccgtt 4200

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc 4260

4201 ctcgttgagc cagcggcgta tgtgataaga gtcttactga accaactcat gagtggtcag 4260

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc 4320

4261 tgtcttttcg tagaatgcct accgtactgt cattctctta atacgtcacg acggtattgg 4320

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta 4380

4321 tactcactat tgtgacgccg gttgaatgaa gactgttgct agcctcctgg cttcctcgat 4380

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag 4440

4381 tggcgaaaaa acgtgttgta ccccctagta cattgagcgg aactagcaac ccttggcctc 4440

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca 4500

4441 gacttacttc ggtatggttt gctgctcgca ctgtggtgct acggacatcg ttaccgttgt 4500

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata 4560

4501 tgcaacgcgt ttgataattg accgcttgat gaatgagatc gaagggccgt tgttaattat 4560

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc 4620

4561 ctgacctacc tccgcctatt tcaacgtcct ggtgaagacg cgagccggga aggccgaccg 4620

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca 4680

4621 accaaataac gactatttag acctcggcca ctcgcaccca gagcgccata gtaacgtcgt 4680

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca 4740

4681 gaccccggtc taccattcgg gagggcatag catcaataga tgtgctgccc ctcagtccgt 4740

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg 4800

4741 tgatacctac ttgctttatc tgtctagcga ctctatccac ggagtgacta attcgtaacc 4800

.......... ....... ORF 1 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa 4860

4801 attgacagtc tggttcaaat gagtatatat gaaatctaac taaattttga agtaaaaatt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt 4920

4861 aaattttcct agatccactt ctaggaaaaa ctattagagt actggtttta gggaattgca 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat 4980

4921 ctcaaaagca aggtgactcg cagtctgggg catcttttct agtttcctag aagaactcta 4980

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

4981 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg 5040

4981 ggaaaaaaag acgcgcatta gacgacgaac gtttgttttt ttggtggcga tggtcgccac 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga 5100

5041 caaacaaacg gcctagttct cgatggttga gaaaaaggct tccattgacc gaagtcgtct 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac 5160

5101 cgcgtctatg gtttatgaca ggaagatcac atcggcatca atccggtggt gaagttcttg 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt 5220

5161 agacatcgtg gcggatgtat ggagcgagac gattaggaca atggtcaccg acgacggtca 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag 5280

5221 ccgctattca gcacagaatg gcccaacctg agttctgcta tcaatggcct attccgcgtc 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc 5340

5281 gccagcccga cttgcccccc aagcacgtgt gtcgggtcga acctcgcttg ctggatgtgg 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag 5400

5341 cttgactcta tggatgtcgc actcgatact ctttcgcggt gcgaagggct tccctctttc 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca 5460

5401 cgcctgtcca taggccattc gccgtcccag ccttgtcctc tcgcgtgctc cctcgaaggt 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt 5520

5461 ccccctttgc ggaccataga aatatcagga cagcccaaag cggtggagac tgaactcgca 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc 5580

5521 gctaaaaaca ctacgagcag tccccccgcc tcggatacct ttttgcggtc gttgcgccgg 5580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc 5640

5581 aaaaatgcca aggaccggaa aacgaccgga aaacgagtgt acaagaaagg acgcaatagg 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc 5700

5641 ggactaagac acctattggc ataatggcgg aaactcactc gactatggcg agcggcgtcg 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa 5760

5701 gcttgctggc tcgcgtcgct cagtcactcg ctccttcgcc ttctcgcggg ttatgcgttt 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac 5820

5761 ggcggagagg ggcgcgcaac cggctaagta attacgtcga ccgtgctgtc caaagggctg 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc 5880

5821 acctttcgcc cgtcactcgc gttgcgttaa ttacactcaa tcgagtgagt aatccgtggg 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa 5940

5881 gtccgaaatg tgaaatacga aggccgagca tacaacacac cttaacactc gcctattgtt 5940

.......... lac .......... .......... .......... M13_pUC_r

.......... .......... .......... .......... .......... ..........

5941 tttcacacag gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa 6000

5941 aaagtgtgtc ctttgtcgat actggtacta atgcggttcg cgcgttaatt gggagtgatt 6000

M13_pUC_r M13_r .......... .......... .......... T3

......... BglII(6020) ......... .......... .......... ..........

6001 agggaacaaa agctggtcga gatctagc.. .......... .......... .......... 6028

6001 tcccttgttt tcgaccagct ctagatcg.. .......... .......... .......... 6028

T3 .......... .......... .......... .......... ..........