Sequence of pFB-Luc

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Sequence of pFB-Luc_(ViraPort)

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Sequence of pFB-Luc (ViraPort) : 6768 BP; 1695 A; 1772 C; 1663 G; 1638 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc 60

1 cttaacgatc gttaacgatc gttaacgatc gttaagtatg gtctagtggc ttttgacagg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta 120

61 aggtttacac agggggagtg tgagggttta agcgcccgaa gacggagaat ctggtgagat 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... SacII(154) .......... ..........

121 ccctattccc cacactcacc ggagccaaag ccgcgggaca tatacatgtg aaagacccca 180

121 gggataaggg gtgtgagtgg cctcggtttc ggcgccctgt atatgtacac tttctggggt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat 240

181 ggacatccaa accgttcgat cgaattcatt gcggtaaaac gttccgtacc tttttatgta 240

.......... ......... 3MoMuLV_LTR ......... 5_LTR2 ..........

.......... .......... .......... .......... .......... ..........

241 aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc 300

241 ttgactctta tcttttcaag tctagttcca gtccttgtct accttgtcga cttatacccg 300

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

301 caaagcggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa 360

301 gtttcgccta tagacaccat tcgtcaagga cggggccgag tcccggttct tgtctacctt 360

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc 420

361 gtcgacttat acccggtttg tcctatagac accattcgtc aaggacgggg ccgagtcccg 420

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga 480

421 gttcttgtct accaggggtc tacgccaggt cgggagtcgt caaagatctc ttggtagtct 480

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc 540

481 acaaaggtcc cacggggttc ctggacttta ctgggacacg gaataaactt gattggttag 540

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

541 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac 600

541 tcaagcgaag agcgaagaca agcgcgcgaa gacgaggggc tcgagttatt ttctcgggtg 600

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc 660

601 ttggggagtg agccccgcgg tcaggaggct aactgactca gcgggcccat gggcacatag 660

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc 720

661 gttatttggg agaacgtcaa cgtaggctga acaccagagc gacaaggaac cctcccagag 720

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg 780

721 gagactcact aactgatggg cagtcgcccc cagaaagtaa acccccgagc aggccctagc 780

...... 3MoMuLV_LTR 5_LTR2 ...... ........ psi_plus_pack2 ........

.......... .......... .......... .......... .......... ..........

781 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta 840

781 cctctgggga cgggtccctg gtggctgggt ggtggccctc cattcgaccg gtcgttgaat 840

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... SpeI(897)

841 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta 900

841 agacacagac aggctaacag atcacagata ctgactaaaa tacgcggacg cagccatgat 900

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc 960

901 caatcgattg atcgagacat agaccgcctg ggcaccacct tgactgctca agccttgtgg 960

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg 1020

961 gccggcgttg ggaccctctg cagggtccct gaagcccccg gcaaaaacac cgggctggac 1020

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc ccccttagag gagggatatg 1080

1021 tcaggttttt agggctagca aaacctgaga aaccacgtgg ggggaatctc ctccctatac 1080

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 1140

1081 accaagacca tcctctgctc ttggattttg tcaagggcgg aggcagactt aaaaacgaaa 1140

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc 1200

1141 gccaaaccct ggcttcggcg cggcgcgcag aacagacgac gtcgtagcaa gacacaacag 1200

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... ApaI(1242) ..........

1201 tctgtctgac tgtgtttctg tatttgtctg aaaatatggg cccgggccag actgttacca 1260

1201 agacagactg acacaaagac ataaacagac ttttataccc gggcccggtc tgacaatggt 1260

.......... ........ psi_plus_pack2 ........ gag ORF 3 ..........

.......... .......... .......... .......... .......... ..........

1261 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcggatcgct cacaaccagt 1320

1261 gagggaattc aaactggaat ccagtgacct ttctacagct cgcctagcga gtgttggtca 1320

.......... ... psi_plus_pack2 gag ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta 1380

1321 gccatctaca gttcttctct gcaacccaat ggaagacgag acgtcttacc ggttggaaat 1380

.......... ... psi_plus_pack2 gag ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca 1440

1381 tgcagcctac cggcgctctg ccgtggaaat tggctctgga gtagtgggtc caattctagt 1440

.......... ... psi_plus_pack2 gag ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg 1500

1441 tccagaaaag tggaccgggc gtacctgtgg gtctggtcca ggggatgtag cactggaccc 1500

.......... ... psi_plus_pack2 gag ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc 1560

1501 ttcggaaccg aaaactgggg ggagggaccc agttcgggaa acatgtggga ttcggaggcg 1560

.......... ... psi_plus_pack2 gag ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc 1620

1561 gaggagaagg aggtaggcgg ggcagagagg gggaacttgg aggagcaagc tggggcggag 1620

.......... . psi_plus_pack2 gag ORF 3 MSCV_pri pLXSN_5_pri .....

.......... .......... .......... .......... ......... BglII(1678)

1621 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga 1680

1621 ctaggaggga aataggtcgg gagtgaggaa gagatccgcg ggggtatacc ggtatactct 1680

.. pBABE_5_pri psi_plus_pack2 gag ORF 3 .. .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta 1740

1681 agaatatacc ccgtgggggc ggggaacatt tgaagggact gggactgtac tgttctcaat 1740

.......... ... psi_plus_pack2 gag ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct 1800

1741 gattgtcggg gagagaggtt cgagtgaatg tccgagagat gaatcaggtc gtgcttcaga 1800

.......... ..... psi_plus_pack2 ORF 3 ..... .......... ..........

.......... .......... .......... AgeI(1840) .......... ..........

1801 ggagacctct ggcggcacgt accaagaaca actggaccga ccggtggtac ctcaccctta 1860

1801 cctctggaga ccgccgtgca tggttcttgt tgacctggct ggccaccatg gagtgggaat 1860

.......... ..... psi_plus_pack2 ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 ccgagtcggc gacacagtgt gggtccgccg acaccagact aagaacctag aacctcgctg 1920

1861 ggctcagccg ctgtgtcaca cccaggcggc tgtggtctga ttcttggatc ttggagcgac 1920

.......... ..... psi_plus_pack2 ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gaaaggacct tacacagtcc tgctgaccac ccccaccgcc ctcaaagtag acggcatcgc 1980

1921 ctttcctgga atgtgtcagg acgactggtg ggggtggcgg gagtttcatc tgccgtagcg 1980

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 agcttggata cacgccgccc acgtgaaggc tgccgacccc gggggtggac catcctctag 2040

1981 tcgaacctat gtgcggcggg tgcacttccg acggctgggg cccccacctg gtaggagatc 2040

pBMN_5_pri ........ psi_plus_pack2 ........ .......... ..........

.......... .... SalI(2057),NcoI(2062) .... .......... ..........

2041 actgccggat cgaattgtcg accatggaag acgccaaaaa cataaagaaa g gcccggcgc 2100

2041 tgacggccta gcttaacagc tggtaccttc tgcggttttt gtatttcttt ccgggccgcg 2100

.......... .......... .......... ... firefly_Luciferase ORF 3 ...

.......... .......... .......... .......... .......... ..........

2101 cattctatcc tctagaggat ggaaccgctg gagagcaact gcataaggct atgaagagat 2160

2101 gtaagatagg agatctccta ccttggcgac ctctcgttga cgtattccga tacttctcta 2160

.......... .. firefly_Luciferase ORF 3 LucNrev_pri .. ..........

.......... .......... .......... .......... .......... ..........

2161 acgccctggt tcctggaaca attgctttta cagatgcaca tatcgaggtg aacatcacgt 2220

2161 tgcgggacca aggaccttgt taacgaaaat gtctacgtgt atagctccac ttgtagtgca 2220

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 acgcggaata cttcgaaatg tccgttcggt tggcagaagc tatgaaacga tatgggctga 2280

2221 tgcgccttat gaagctttac aggcaagcca accgtcttcg atactttgct atacccgact 2280

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 atacaaatca cagaatcgtc gtatgcagtg aaaactctct tcaattcttt atgccggtgt 2340

2281 tatgtttagt gtcttagcag catacgtcac ttttgagaga agttaagaaa tacggccaca 2340

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 tgggcgcgtt atttatcgga gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg 2400

2341 acccgcgcaa taaatagcct caacgtcaac gcgggcgctt gctgtaaata ttacttgcac 2400

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 aattgctcaa cagtatgaac atttcgcagc ctaccgtagt gtttgtttcc aaaaaggggt 2460

2401 ttaacgagtt gtcatacttg taaagcgtcg gatggcatca caaacaaagg tttttcccca 2460

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgcaaaaaat tttgaacgtg caaaaaaaat taccaataat ccagaaaatt attatcatgg 2520

2461 acgtttttta aaacttgcac gtttttttta atggttatta ggtcttttaa taatagtacc 2520

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 attctaaaac ggattaccag ggatttcagt cgatgtacac gttcgtcaca tctcatctac 2580

2521 taagattttg cctaatggtc cctaaagtca gctacatgtg caagcagtgt agagtagatg 2580

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ctcccggttt taatgaatac gattttgtac cagagtcctt tgatcgtgac aaaacaattg 2640

2581 gagggccaaa attacttatg ctaaaacatg gtctcaggaa actagcactg ttttgttaac 2640

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

......... EcoRI(2651) ......... .......... .......... ..........

2641 cactgataat gaattcctct ggatctactg ggttacctaa gggtgtggcc cttccgcata 2700

2641 gtgactatta cttaaggaga cctagatgac ccaatggatt cccacaccgg gaaggcgtat 2700

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gaactgcctg cgtcagattc tcgcatgcca gagatcctat ttttggcaat caaatcattc 2760

2701 cttgacggac gcagtctaag agcgtacggt ctctaggata aaaaccgtta gtttagtaag 2760

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cggatactgc gattttaagt gttgttccat tccatcacgg ttttggaatg tttactacac 2820

2761 gcctatgacg ctaaaattca caacaaggta aggtagtgcc aaaaccttac aaatgatgtg 2820

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 tcggatattt gatatgtgga tttcgagtcg tcttaatgta tagatttgaa gaagagctgt 2880

2821 agcctataaa ctatacacct aaagctcagc agaattacat atctaaactt cttctcgaca 2880

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 ttttacgatc ccttcaggat tacaaaattc aaagtgcgtt gctagtacca accctatttt 2940

2881 aaaatgctag ggaagtccta atgttttaag tttcacgcaa cgatcatggt tgggataaaa 2940

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 cattcttcgc caaaagcact ctgattgaca aatacgattt atctaattta cacgaaattg 3000

2941 gtaagaagcg gttttcgtga gactaactgt ttatgctaaa tagattaaat gtgctttaac 3000

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 cttctggggg cgcacctctt tcgaaagaag tcggggaagc ggttgcaaaa cgcttccatc 3060

3001 gaagaccccc gcgtggagaa agctttcttc agccccttcg ccaacgtttt gcgaaggtag 3060

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 ttccagggat acgacaagga tatgggctca ctgagactac atcagctatt ctgattacac 3120

3061 aaggtcccta tgctgttcct atacccgagt gactctgatg tagtcgataa gactaatgtg 3120

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccgaggggga tgataaaccg ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg 3180

3121 ggctccccct actatttggc ccgcgccagc catttcaaca aggtaaaaaa cttcgcttcc 3180

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttgtggatct ggataccggg aaaacgctgg gcgttaatca gagaggcgaa ttatgtgtca 3240

3181 aacacctaga cctatggccc ttttgcgacc cgcaattagt ctctccgctt aatacacagt 3240

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gaggacctat gattatgtcc ggttatgtaa acaatccgga agcgaccaac gccttgattg 3300

3241 ctcctggata ctaatacagg ccaatacatt tgttaggcct tcgctggttg cggaactaac 3300

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 acaaggatgg atggctacat tctggagaca tagcttactg ggacgaagac gaacacttct 3360

3301 tgttcctacc taccgatgta agacctctgt atcgaatgac cctgcttctg cttgtgaaga 3360

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcatagttga ccgcttgaag tctttaatta aatacaaagg atatcaggtg gcccccgctg 3420

3361 agtatcaact ggcgaacttc agaaattaat ttatgtttcc tatagtccac cgggggcgac 3420

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

ClaI(3429) .......... .......... .......... .......... ..........

3421 aattggaatc gatattgtta caacacccca acatcttcga cgcgggcgtg gcaggtcttc 3480

3421 ttaaccttag ctataacaat gttgtggggt tgtagaagct gcgcccgcac cgtccagaag 3480

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ccgacgatga cgccggtgaa cttcccgccg ccgttgttgt tttggagcac ggaaagacga 3540

3481 ggctgctact gcggccactt gaagggcggc ggcaacaaca aaacctcgtg cctttctgct 3540

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tgacggaaaa agagatcgtg gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc 3600

3541 actgcctttt tctctagcac ctaatgcagc ggtcagttca ttgttggcgc tttttcaacg 3600

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gcggaggagt tgtgtttgtg gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa 3660

3601 cgcctcctca acacaaacac ctgcttcatg gctttccaga atggcctttt gagctgcgtt 3660

.......... ... firefly_Luciferase ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gaaaaatcag agagatcctc ataaaggcca agaagggcgg aaagtccaaa ttgtaatgag 3720

3661 ctttttagtc tctctaggag tatttccggt tcttcccgcc tttcaggttt aacattactc 3720

...... firefly_Luciferase ORF 3 ...... .......... ..........

XhoI(3725) NotI(3731) .......... .......... .......... ..........

3721 gatcctcgag cggccgcgat ccgagttctt ctgagcggga ctctggggtt cgataaaata 3780

3721 ctaggagctc gccggcgcta ggctcaagaa gactcgccct gagaccccaa gctattttat 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 aaagatttta tttagtctcc agaaaaaggg gggaatgaaa gaccccacct gtaggtttgg 3840

3781 tttctaaaat aaatcagagg tctttttccc cccttacttt ctggggtgga catccaaacc 3840

.......... .......... .......... ......... 3MoMuLV_LTR .........

.......... .......... .......... .......... .......... ..........

3841 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga 3900

3841 gttcgatcga attcattgcg gtaaaacgtt ccgtaccttt ttatgtattg actcttatct 3900

.......... ......... 3MoMuLV_LTR 5_LTR2 ......... ..........

.......... .......... .......... .......... .......... ..........

3901 gaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc 3960

3901 cttcaagtct agttccagtc cttgtctacc ttgtcgactt atacccggtt tgtcctatag 3960

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg 4020

3961 acaccattcg tcaaggacgg ggccgagtcc cggttcttgt ctaccttgtc gacttatacc 4020

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg 4080

4021 cggtttgtcc tatagacacc attcgtcaag gacggggccg agtcccggtt cttgtctacc 4080

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg 4140

4081 aggggtctac gccaggtcgg gagtcgtcaa agatctcttg gtagtctaca aaggtcccac 4140

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg 4200

4141 ggggttcctg gactttactg ggacacggaa taaacttgat tggttagtca agcgaagagc 4200

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg 4260

4201 gaagacaagc gcgcgaagac gaggggctcg agttattttc tcgggtgttg gggagtgagc 4260

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gggcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct 4320

4261 cccgcggtca ggaggctaac tgactcagcg ggcccatggg cacataggtt atttgggaga 4320

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg 4380

4321 acgtcaacgt aggctgaaca ccagagcgac aaggaaccct cccagaggag actcactaac 4380

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc 4440

4381 tgatgggcag tcgcccccag aaagtaaacc cccgagcagg ccctagccct ctggggacgg 4440

...... 3MoMuLV_LTR 5_LTR2 ...... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cagggaccac cgacccacca ccgggaggta agctggctgc ctcgcgcgtt tcggtgatga 4500

4441 gtccctggtg gctgggtggt ggccctccat tcgaccgacg gagcgcgcaa agccactact 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 cggtgaaaac ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga 4560

4501 gccacttttg gagactgtgt acgtcgaggg cctctgccag tgtcgaacag acattcgcct 4560

.......... .......... pGEX_3_pri .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc 4620

4561 acggccctcg tctgttcggg cagtcccgcg cagtcgccca caaccgccca cagccccgcg 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta tgcggcatca 4680

4621 tcggtactgg gtcagtgcat cgctatcgcc tcacatatga ccgaattgat acgccgtagt 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg 4740

4681 ctcgtctaac atgactctca cgtggtatac gccacacttt atggcgtgtc tacgcattcc 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc 4800

4741 tcttttatgg cgtagtccgc gagaaggcga aggagcgagt gactgagcga cgcgagccag 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 4860

4801 caagccgacg ccgctcgcca tagtcgagtg agtttccgcc attatgccaa taggtgtctt 4860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 4920

4861 agtcccctat tgcgtccttt cttgtacact cgttttccgg tcgttttccg gtccttggca 4920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 4980

4921 tttttccggc gcaacgaccg caaaaaggta tccgaggcgg ggggactgct cgtagtgttt 4980

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

4981 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 5040

4981 ttagctgcga gttcagtctc caccgctttg ggctgtcctg atatttctat ggtccgcaaa 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 5100

5041 gggggacctt cgagggagca cgcgagagga caaggctggg acggcgaatg gcctatggac 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 5160

5101 aggcggaaag agggaagccc ttcgcaccgc gaaagagtat cgagtgcgac atccatagag 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 5220

5161 tcaagccaca tccagcaagc gaggttcgac ccgacacacg tgcttggggg gcaagtcggg 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta 5280

5221 ctggcgacgc ggaataggcc attgatagca gaactcaggt tgggccattc tgtgctgaat 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 5340

5281 agcggtgacc gtcgtcggtg accattgtcc taatcgtctc gctccataca tccgccacga 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc 5400

5341 tgtctcaaga acttcaccac cggattgatg ccgatgtgat cttcctgtca taaaccatag 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 5460

5401 acgcgagacg acttcggtca atggaagcct ttttctcaac catcgagaac taggccgttt 5460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5461 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 5520

5461 gtttggtggc gaccatcgcc accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt 5520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 5580

5521 tttcctagag ttcttctagg aaactagaaa agatgcccca gactgcgagt caccttgctt 5580

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt 5640

5581 ttgagtgcaa ttccctaaaa ccagtactct aatagttttt cctagaagtg gatctaggaa 5640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac 5700

5641 aatttaattt ttacttcaaa atttagttag atttcatata tactcatttg aaccagactg 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc 5760

5701 tcaatggtta cgaattagtc actccgtgga tagagtcgct agacagataa agcaagtagg 5760

.......... .......... ....... ORF 3 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

5761 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc 5820

5761 tatcaacgga ctgaggggca gcacatctat tgatgctatg ccctcccgaa tggtagaccg 5820

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata 5880

5821 gggtcacgac gttactatgg cgctctgggt gcgagtggcc gaggtctaaa tagtcgttat 5880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc 5940

5881 ttggtcggtc ggccttcccg gctcgcgtct tcaccaggac gttgaaatag gcggaggtag 5940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

AseI(5948) .......... .......... .......... .......... FspI(5998)

5941 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc 6000

5941 gtcagataat taacaacggc ccttcgatct cattcatcaa gcggtcaatt atcaaacgcg 6000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca 6060

6001 ttgcaacaac ggtaacgacg tccgtagcac cacagtgcga gcagcaaacc ataccgaagt 6060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa 6120

6061 aagtcgaggc caagggttgc tagttccgct caatgtacta gggggtacaa cacgtttttt 6120

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca 6180

6121 cgccaatcga ggaagccagg aggctagcaa cagtcttcat tcaaccggcg tcacaatagt 6180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt 6240

6181 gagtaccaat accgtcgtga cgtattaaga gaatgacagt acggtaggca ttctacgaaa 6240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt 6300

6241 agacactgac cactcatgag ttggttcagt aagactctta tcacatacgc cgctggctca 6300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg 6360

6301 acgagaacgg gccgcagttg tgccctatta tggcgcggtg tatcgtcttg aaattttcac 6360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga 6420

6361 gagtagtaac cttttgcaag aagccccgct tttgagagtt cctagaatgg cgacaactct 6420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc 6480

6421 aggtcaagct acattgggtg agcacgtggg ttgactagaa gtcgtagaaa atgaaagtgg 6480

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg 6540

6481 tcgcaaagac ccactcgttt ttgtccttcc gttttacggc gttttttccc ttattcccgc 6540

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag 6600

6541 tgtgccttta caacttatga gtatgagaag gaaaaagtta taataacttc gtaaatagtc 6600

....... ORF 3 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg 6660

6601 ccaataacag agtactcgcc tatgtataaa cttacataaa tctttttatt tgtttatccc 6660

.......... AmpR_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg 6720

6661 caaggcgcgt gtaaaggggc ttttcacggt ggactgcaga ttctttggta ataatagtac 6720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaa.. .......... 6768

6721 tgtaattgga tatttttatc cgcatagtgc tccgggaaag cagaagtt.. .......... 6768

.......... .......... .......... .......... .......... ..........