Sequence of pFB-Neo

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Sequence of pFB-Neo_(ViraPort)

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Sequence of pFB-Neo (ViraPort) : 6572 BP; 1513 A; 1840 C; 1685 G; 1534 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 gaattgctag caattgctag caattgctag caattcatac cagatcaccg aaaactgtcc 60

1 cttaacgatc gttaacgatc gttaacgatc gttaagtatg gtctagtggc ttttgacagg 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tccaaatgtg tccccctcac actcccaaat tcgcgggctt ctgcctctta gaccactcta 120

61 aggtttacac agggggagtg tgagggttta agcgcccgaa gacggagaat ctggtgagat 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... SacII(154) .......... ..........

121 ccctattccc cacactcacc ggagccaaag ccgcgggaca tatacatgtg aaagacccca 180

121 gggataaggg gtgtgagtgg cctcggtttc ggcgccctgt atatgtacac tttctggggt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cctgtaggtt tggcaagcta gcttaagtaa cgccattttg caaggcatgg aaaaatacat 240

181 ggacatccaa accgttcgat cgaattcatt gcggtaaaac gttccgtacc tttttatgta 240

.......... ......... 3MoMuLV_LTR ......... 5_LTR2 ..........

.......... .......... .......... .......... .......... ..........

241 aactgagaat agaaaagttc agatcaaggt caggaacaga tggaacagct gaatatgggc 300

241 ttgactctta tcttttcaag tctagttcca gtccttgtct accttgtcga cttatacccg 300

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

301 caaagcggat atctgtggta agcagttcct gccccggctc agggccaaga acagatggaa 360

301 gtttcgccta tagacaccat tcgtcaagga cggggccgag tcccggttct tgtctacctt 360

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cagctgaata tgggccaaac aggatatctg tggtaagcag ttcctgcccc ggctcagggc 420

361 gtcgacttat acccggtttg tcctatagac accattcgtc aaggacgggg ccgagtcccg 420

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

421 caagaacaga tggtccccag atgcggtcca gccctcagca gtttctagag aaccatcaga 480

421 gttcttgtct accaggggtc tacgccaggt cgggagtcgt caaagatctc ttggtagtct 480

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

481 tgtttccagg gtgccccaag gacctgaaat gaccctgtgc cttatttgaa ctaaccaatc 540

481 acaaaggtcc cacggggttc ctggacttta ctgggacacg gaataaactt gattggttag 540

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

541 agttcgcttc tcgcttctgt tcgcgcgctt ctgctccccg agctcaataa aagagcccac 600

541 tcaagcgaag agcgaagaca agcgcgcgaa gacgaggggc tcgagttatt ttctcgggtg 600

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

601 aacccctcac tcggggcgcc agtcctccga ttgactgagt cgcccgggta cccgtgtatc 660

601 ttggggagtg agccccgcgg tcaggaggct aactgactca gcgggcccat gggcacatag 660

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

661 caataaaccc tcttgcagtt gcatccgact tgtggtctcg ctgttccttg ggagggtctc 720

661 gttatttggg agaacgtcaa cgtaggctga acaccagagc gacaaggaac cctcccagag 720

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ctctgagtga ttgactaccc gtcagcgggg gtctttcatt tgggggctcg tccgggatcg 780

721 gagactcact aactgatggg cagtcgcccc cagaaagtaa acccccgagc aggccctagc 780

...... 3MoMuLV_LTR 5_LTR2 ...... ........ psi_plus_pack2 ........

.......... .......... .......... .......... .......... ..........

781 ggagacccct gcccagggac caccgaccca ccaccgggag gtaagctggc cagcaactta 840

781 cctctgggga cgggtccctg gtggctgggt ggtggccctc cattcgaccg gtcgttgaat 840

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... SpeI(897)

841 tctgtgtctg tccgattgtc tagtgtctat gactgatttt atgcgcctgc gtcggtacta 900

841 agacacagac aggctaacag atcacagata ctgactaaaa tacgcggacg cagccatgat 900

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

901 gttagctaac tagctctgta tctggcggac ccgtggtgga actgacgagt tcggaacacc 960

901 caatcgattg atcgagacat agaccgcctg ggcaccacct tgactgctca agccttgtgg 960

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

961 cggccgcaac cctgggagac gtcccaggga cttcgggggc cgtttttgtg gcccgacctg 1020

961 gccggcgttg ggaccctctg cagggtccct gaagcccccg gcaaaaacac cgggctggac 1020

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1021 agtccaaaaa tcccgatcgt tttggactct ttggtgcacc ccccttagag gagggatatg 1080

1021 tcaggttttt agggctagca aaacctgaga aaccacgtgg ggggaatctc ctccctatac 1080

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tggttctggt aggagacgag aacctaaaac agttcccgcc tccgtctgaa tttttgcttt 1140

1081 accaagacca tcctctgctc ttggattttg tcaagggcgg aggcagactt aaaaacgaaa 1140

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1141 cggtttggga ccgaagccgc gccgcgcgtc ttgtctgctg cagcatcgtt ctgtgttgtc 1200

1141 gccaaaccct ggcttcggcg cggcgcgcag aacagacgac gtcgtagcaa gacacaacag 1200

.......... ........ psi_plus_pack2 ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tctgtctgac tgtgtttctg tatttgtctg aaaatatggg cccgggccag actgttacca 1260

1201 agacagactg acacaaagac ataaacagac ttttataccc gggcccggtc tgacaatggt 1260

.......... ........ psi_plus_pack2 ........ gag ORF 3 ..........

.......... .......... .......... .......... .......... ..........

1261 ctcccttaag tttgacctta ggtcactgga aagatgtcga gcagatcgct cacaaccagt 1320

1261 gagggaattc aaactggaat ccagtgacct ttctacagct cgtctagcga gtgttggtca 1320

.......... ... psi_plus_pack2 gag ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 cggtagatgt caagaagaga cgttgggtta ccttctgctc tgcagaatgg ccaaccttta 1380

1321 gccatctaca gttcttctct gcaacccaat ggaagacgag acgtcttacc ggttggaaat 1380

.......... ... psi_plus_pack2 gag ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 acgtcggatg gccgcgagac ggcaccttta accgagacct catcacccag gttaagatca 1440

1381 tgcagcctac cggcgctctg ccgtggaaat tggctctgga gtagtgggtc caattctagt 1440

.......... ... psi_plus_pack2 gag ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 aggtcttttc acctggcccg catggacacc cagaccaggt cccctacatc gtgacctggg 1500

1441 tccagaaaag tggaccgggc gtacctgtgg gtctggtcca ggggatgtag cactggaccc 1500

.......... ... psi_plus_pack2 gag ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aagccttggc ttttgacccc cctccctggg tcaagccctt tgtacaccct aagcctccgc 1560

1501 ttcggaaccg aaaactgggg ggagggaccc agttcgggaa acatgtggga ttcggaggcg 1560

.......... ... psi_plus_pack2 gag ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 ctcctcttcc tccatccgcc ccgtctctcc cccttgaacc tcctcgttcg accccgcctc 1620

1561 gaggagaagg aggtaggcgg ggcagagagg gggaacttgg aggagcaagc tggggcggag 1620

.......... . psi_plus_pack2 gag ORF 3 MSCV_pri pLXSN_5_pri .....

.......... .......... .......... .......... ......... BglII(1678)

1621 gatcctccct ttatccagcc ctcactcctt ctctaggcgc ccccatatgg ccatatgaga 1680

1621 ctaggaggga aataggtcgg gagtgaggaa gagatccgcg ggggtatacc ggtatactct 1680

.. pBABE_5_pri psi_plus_pack2 gag ORF 3 .. .......... ..........

.......... .......... .......... .......... .......... ..........

1681 tcttatatgg ggcacccccg ccccttgtaa acttccctga ccctgacatg acaagagtta 1740

1681 agaatatacc ccgtgggggc ggggaacatt tgaagggact gggactgtac tgttctcaat 1740

.......... ... psi_plus_pack2 gag ORF 3 ... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctaacagccc ctctctccaa gctcacttac aggctctcta cttagtccag cacgaagtct 1800

1741 gattgtcggg gagagaggtt cgagtgaatg tccgagagat gaatcaggtc gtgcttcaga 1800

.......... ..... psi_plus_pack2 ORF 3 ..... .......... ..........

.......... .......... .......... .......... AgeI(1841) ..........

1801 ggagacctct ggcggcagcc taccaagaac aactggaccg accggtggta cctcaccctt 1860

1801 cctctggaga ccgccgtcgg atggttcttg ttgacctggc tggccaccat ggagtgggaa 1860

.......... ..... psi_plus_pack2 ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 accgagtcgg cgacacagtg tgggtccgcc gacaccagac taagaaccta gaacctcgct 1920

1861 tggctcagcc gctgtgtcac acccaggcgg ctgtggtctg attcttggat cttggagcga 1920

.......... ..... psi_plus_pack2 ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggaaaggacc ttacacagtc ctgctgacca cccccaccgc cctcaaagta gacggcatcg 1980

1921 cctttcctgg aatgtgtcag gacgactggt gggggtggcg ggagtttcat ctgccgtagc 1980

.......... ..... psi_plus_pack2 ORF 3 ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cagcttggat acacgccgcc cacgtgaagg ctgccgaccc cgggggtgga ccatcctcta 2040

1981 gtcgaaccta tgtgcggcgg gtgcacttcc gacggctggg gcccccacct ggtaggagat 2040

..... pBMN_5_pri psi_plus_pack2 ORF 3 ..... .......... ..........

... SalI EcoRI2064) BamHI(2070),XhoI(2075) NotI(2081) ...

2041 gactgccgga tcgaattgtc gacgaattcg gatcctcgag cggccgcgat ccggttattt 2100

2041 ctgacggcct agcttaacag ctgcttaagc ctaggagctc gccggcgcta ggccaataaa 2100

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg 2160

2101 aggtggtata acggcagaaa accgttacac tcccgggcct ttggaccggg acagaagaac 2160

.......... .......... ORF 3 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc 2220

2161 tgctcgtaag gatccccaga aaggggagag cggtttcctt acgttccaga caacttacag 2220

ORF 3 .......... .......... .......... .......... ..........

.......... ........ HindIII(2243) ........ .......... ..........

2221 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt 2280

2221 cacttccttc gtcaaggaga ccttcgaaga acttctgttt gttgcagaca tcgctgggaa 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta 2340

2281 acgtccgtcg ccttgggggg tggaccgctg tccacggaga cgccggtttt cggtgcacat 2340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg 2400

2341 attctatgtg gacgtttccg ccgtgttggg gtcacggtgc aacactcaac ctatcaacac 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag 2460

2401 ctttctcagt ttaccgagag gagttcgcat aagttgttcc ccgacttcct acgggtcttc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag 2520

2461 catggggtaa cataccctag actagacccc ggagccacgt gtacgaaatg tacacaaatc 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tcgaggttaa aaaacgtcta ggccccccga accacgggga cgtggttttc ctttgaaaaa 2580

2521 agctccaatt ttttgcagat ccggggggct tggtgcccct gcaccaaaag gaaacttttt 2580

.......... .......... .......... .......... .......... ..........

ClaI(2586) .......... .......... .......... .......... ..........

2581 cacgatcgat aatatggaac aaaaacttat ttctgaagaa gacttggaca ccaaactttc 2640

2581 gtgctagcta ttataccttg tttttgaata aagacttctt ctgaacctgt ggtttgaaag 2640

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 ctgccgctcg atttctccac ccaggtgaac tcctccctca cctccccgac ggggcgaggc 2700

2641 gacggcgagc taaagaggtg ggtccacttg aggagggagt ggaggggctg ccccgctccg 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tccatggctg ccccctcgct gcacccgtcc ctggtgccac gcggttccat gggatcgttt 2760

2701 aggtaccgac gggggagcga cgtgggcagg gaccacggtg cgccaaggta ccctagcaaa 2760

.......... .......... .......... ORF 1 .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cgcatgattg aacaagatgg attgcacgca ggttctccgg ccgcttgggt ggagaggcta 2820

2761 gcgtactaac ttgttctacc taacgtgcgt ccaagaggcc ggcgaaccca cctctccgat 2820

.......... .......... ORF 1 Neomycin .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ttcggctatg actgggcaca acagacaatc ggctgctctg atgccgccgt gttccggctg 2880

2821 aagccgatac tgacccgtgt tgtctgttag ccgacgagac tacggcggca caaggccgac 2880

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa 2940

2881 agtcgcgtcc ccgcgggcca agaaaaacag ttctggctgg acaggccacg ggacttactt 2940

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct 3000

2941 gacgtcctgc tccgtcgcgc cgatagcacc gaccggtgct gcccgcaagg aacgcgtcga 3000

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gtgctcgacg ttgtcactga agcgggaagg gactggctgc tattgggcga agtgccgggg 3060

3001 cacgagctgc aacagtgact tcgcccttcc ctgaccgacg ataacccgct tcacggcccc 3060

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3061 caggatctcc tgtcatctca ccttgctcct gccgagaaag tatccatcat ggctgatgca 3120

3061 gtcctagagg acagtagagt ggaacgagga cggctctttc ataggtagta ccgactacgt 3120

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3121 atgcggcggc tgcatacgct tgatccggct acctgcccat tcgaccacca agcgaaacat 3180

3121 tacgccgccg acgtatgcga actaggccga tggacgggta agctggtggt tcgctttgta 3180

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3181 cgcatcgagc gagcacgtac tcggatggaa gccggtcttg tcgatcagga tgatctggac 3240

3181 gcgtagctcg ctcgtgcatg agcctacctt cggccagaac agctagtcct actagacctg 3240

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3241 gaagagcatc aggggctcgc gccagccgaa ctgttcgcca ggctcaaggc gcgcatgccc 3300

3241 cttctcgtag tccccgagcg cggtcggctt gacaagcggt ccgagttccg cgcgtacggg 3300

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gacggcgagg atctcgtcgt gacccatggc gatgcctgct tgccgaatat catggtggaa 3360

3301 ctgccgctcc tagagcagca ctgggtaccg ctacggacga acggcttata gtaccacctt 3360

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3361 aatggccgct tttctggatt catcgactgt ggccggctgg gtgtggcgga ccgctatcag 3420

3361 ttaccggcga aaagacctaa gtagctgaca ccggccgacc cacaccgcct ggcgatagtc 3420

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gacatagcgt tggctacccg tgatattgct gaagagcttg gcggcgaatg ggctgaccgc 3480

3421 ctgtatcgca accgatgggc actataacga cttctcgaac cgccgcttac ccgactggcg 3480

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ttcctcgtgc tttacggtat cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt 3540

3481 aaggagcacg aaatgccata gcggcgaggg ctaagcgtcg cgtagcggaa gatagcggaa 3540

.......... ........ ORF 1 Neomycin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cttgacgagt tcttctgagc gggactctgg ggttcgataa aataaaagat tttatttagt 3600

3541 gaactgctca agaagactcg ccctgagacc ccaagctatt ttattttcta aaataaatca 3600

ORF 1 Neomycin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 ctccagaaaa aggggggaat gaaagacccc acctgtaggt ttggcaagct agcttaagta 3660

3601 gaggtctttt tcccccctta ctttctgggg tggacatcca aaccgttcga tcgaattcat 3660

.......... .......... ......... 3MoMuLV_LTR ......... ..........

.......... .......... .......... .......... .......... ..........

3661 acgccatttt gcaaggcatg gaaaaataca taactgagaa tagagaagtt cagatcaagg 3720

3661 tgcggtaaaa cgttccgtac ctttttatgt attgactctt atctcttcaa gtctagttcc 3720

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 tcaggaacag atggaacagc tgaatatggg ccaaacagga tatctgtggt aagcagttcc 3780

3721 agtccttgtc taccttgtcg acttataccc ggtttgtcct atagacacca ttcgtcaagg 3780

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 tgccccggct cagggccaag aacagatgga acagctgaat atgggccaaa caggatatct 3840

3781 acggggccga gtcccggttc ttgtctacct tgtcgactta tacccggttt gtcctataga 3840

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gtggtaagca gttcctgccc cggctcaggg ccaagaacag atggtcccca gatgcggtcc 3900

3841 caccattcgt caaggacggg gccgagtccc ggttcttgtc taccaggggt ctacgccagg 3900

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agccctcagc agtttctaga gaaccatcag atgtttccag ggtgccccaa ggacctgaaa 3960

3901 tcgggagtcg tcaaagatct cttggtagtc tacaaaggtc ccacggggtt cctggacttt 3960

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 tgaccctgtg ccttatttga actaaccaat cagttcgctt ctcgcttctg ttcgcgcgct 4020

3961 actgggacac ggaataaact tgattggtta gtcaagcgaa gagcgaagac aagcgcgcga 4020

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tctgctcccc gagctcaata aaagagccca caacccctca ctcggggcgc cagtcctccg 4080

4021 agacgagggg ctcgagttat tttctcgggt gttggggagt gagccccgcg gtcaggaggc 4080

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 attgactgag tcgcccgggt acccgtgtat ccaataaacc ctcttgcagt tgcatccgac 4140

4081 taactgactc agcgggccca tgggcacata ggttatttgg gagaacgtca acgtaggctg 4140

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ttgtggtctc gctgttcctt gggagggtct cctctgagtg attgactacc cgtcagcggg 4200

4141 aacaccagag cgacaaggaa ccctcccaga ggagactcac taactgatgg gcagtcgccc 4200

.......... ...... 3MoMuLV_LTR 5_LTR2 ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ggtctttcat ttgggggctc gtccgggatc gggagacccc tgcccaggga ccaccgaccc 4260

4201 ccagaaagta aacccccgag caggccctag ccctctgggg acgggtccct ggtggctggg 4260

3MoMuLV_LTR 5_LTR2 .. .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 accaccggga ggtaagctgg ctgcctcgcg cgtttcggtg atgacggtga aaacctctga 4320

4261 tggtggccct ccattcgacc gacggagcgc gcaaagccac tactgccact tttggagact 4320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa 4380

4321 gtgtacgtcg agggcctctg ccagtgtcga acagacattc gcctacggcc ctcgtctgtt 4380

pGEX_3_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat gacccagtca 4440

4381 cgggcagtcc cgcgcagtcg cccacaaccg cccacagccc cgcgtcggta ctgggtcagt 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag attgtactga 4500

4441 gcatcgctat cgcctcacat atgaccgaat tgatacgccg tagtctcgtc taacatgact 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa taccgcatca 4560

4501 ctcacgtggt atacgccaca ctttatggcg tgtctacgca ttcctctttt atggcgtagt 4560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag 4620

4561 ccgcgagaag gcgaaggagc gagtgactga gcgacgcgag ccagcaagcc gacgccgctc 4620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag 4680

4621 gccatagtcg agtgagtttc cgccattatg ccaataggtg tcttagtccc ctattgcgtc 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc 4740

4681 ctttcttgta cactcgtttt ccggtcgttt tccggtcctt ggcatttttc cggcgcaacg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc 4800

4741 accgcaaaaa ggtatccgag gcggggggac tgctcgtagt gtttttagct gcgagttcag 4800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4801 agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc 4860

4801 tctccaccgc tttgggctgt cctgatattt ctatggtccg caaaggggga ccttcgaggg 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt 4920

4861 agcacgcgag aggacaaggc tgggacggcg aatggcctat ggacaggcgg aaagagggaa 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg 4980

4921 gcccttcgca ccgcgaaaga gtatcgagtg cgacatccat agagtcaagc cacatccagc 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat 5040

4981 aagcgaggtt cgacccgaca cacgtgcttg gggggcaagt cgggctggcg acgcggaata 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag 5100

5041 ggccattgat agcagaactc aggttgggcc attctgtgct gaatagcggt gaccgtcgtc 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt 5160

5101 ggtgaccatt gtcctaatcg tctcgctcca tacatccgcc acgatgtctc aagaacttca 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc 5220

5161 ccaccggatt gatgccgatg tgatcttcct gtcataaacc atagacgcga gacgacttcg 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta 5280

5221 gtcaatggaa gcctttttct caaccatcga gaactaggcc gtttgtttgg tggcgaccat 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag 5340

5281 cgccaccaaa aaaacaaacg ttcgtcgtct aatgcgcgtc tttttttcct agagttcttc 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga 5400

5341 taggaaacta gaaaagatgc cccagactgc gagtcacctt gcttttgagt gcaattccct 5400

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa 5460

5401 aaaaccagta ctctaatagt ttttcctaga agtggatcta ggaaaattta atttttactt 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa 5520

5461 caaaatttag ttagatttca tatatactca tttgaaccag actgtcaatg gttacgaatt 5520

.......... .......... .......... .......... ....... ORF 2 Ampicillin

.......... .......... .......... .......... .......... ..........

5521 tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc 5580

5521 agtcactccg tggatagagt cgctagacag ataaagcaag taggtatcaa cggactgagg 5580

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga 5640

5581 ggcagcacat ctattgatgc tatgccctcc cgaatggtag accggggtca cgacgttact 5640

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa 5700

5641 atggcgctct gggtgcgagt ggccgaggtc taaatagtcg ttatttggtc ggtcggcctt 5700

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... AseI(5752)

5701 gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt 5760

5701 cccggctcgc gtcttcacca ggacgttgaa ataggcggag gtaggtcaga taattaacaa 5760

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttgccattg 5820

5761 cggcccttcg atctcattca tcaagcggtc aattatcaaa cgcgttgcaa caacggtaac 5820

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ctgcaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc 5880

5821 gacgtccgta gcaccacagt gcgagcagca aaccataccg aagtaagtcg aggccaaggg 5880

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg 5940

5881 ttgctagttc cgctcaatgt actagggggt acaacacgtt ttttcgccaa tcgaggaagc 5940

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag 6000

5941 caggaggcta gcaacagtct tcattcaacc ggcgtcacaa tagtgagtac caataccgtc 6000

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt 6060

6001 gtgacgtatt aagagaatga cagtacggta ggcattctac gaaaagacac tgaccactca 6060

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 actcaaccaa gtcattctga gaatagtgta tgcggcgacc gagttgctct tgcccggcgt 6120

6061 tgagttggtt cagtaagact cttatcacat acgccgctgg ctcaacgaga acgggccgca 6120

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 caacacggga taataccgcg ccacatagca gaactttaaa agtgctcatc attggaaaac 6180

6121 gttgtgccct attatggcgc ggtgtatcgt cttgaaattt tcacgagtag taaccttttg 6180

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac 6240

6181 caagaagccc cgcttttgag agttcctaga atggcgacaa ctctaggtca agctacattg 6240

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag 6300

6241 ggtgagcacg tgggttgact agaagtcgta gaaaatgaaa gtggtcgcaa agacccactc 6300

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa 6360

6301 gtttttgtcc ttccgtttta cggcgttttt tcccttattc ccgctgtgcc tttacaactt 6360

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga 6420

6361 atgagtatga gaaggaaaaa gttataataa cttcgtaaat agtcccaata acagagtact 6420

.......... .......... .......... .......... .......... AmpR_pro

.......... .......... .......... .......... .......... ..........

6421 gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc 6480

6421 cgcctatgta taaacttaca taaatctttt tatttgttta tccccaaggc gcgtgtaaag 6480

AmpR_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa 6540

6481 gggcttttca cggtggactg cagattcttt ggtaataata gtactgtaat tggatatttt 6540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 ataggcgtat cacgaggccc tttcgtcttc aa........ .......... .......... 6572

6541 tatccgcata gtgctccggg aaagcagaag tt........ .......... .......... 6572

.......... .......... .......... .......... .......... ..........