Ø8¤Øp¤<ÿÓLOCUS pFLD 4409 bp DNA SYN 28-Aug-2007 DEFINITION pFLD ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..4409 /organism="pFLD" /mol_type="other DNA" misc_feature 679..720 /label="V5" misc_feature 730..747 /label="6xHis" misc_feature complement(833..853) /label="3_AOX1_primer" misc_feature 1294..1316 /label="pGEX_3_primer" promoter 1475..1503 /label="AmpR_promoter" CDS 1545..2405 /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 3" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene 1545..2405 /label="Ampicillin" /gene="Ampicillin" rep_origin 2560..3179 /label="pBR322_origin" gene 3698..4069 /label="bleo" /gene="bleo" misc_feature 3698..4072 /label="sh_ble" misc_feature complement(4154..4172) /label="CYC1_primer" misc_feature complement(4154..4172) /label="pYESTrp_rev_primer" terminator 4109..4390 /label="CYC1_terminator" ORIGIN 1 GCATGCAGGA ATCTCTGGCA CGGTGCTAAT GGTAGTTATC CAACGGAGCT GAGGTAGTCG 61 ATATATCTGG ATATGCCGCC TATAGGATAA AAACAGGAGA GGGTGAACCT TGCTTATGGC 121 TACTAGATTG TTCTTGTACT CTGAATTCTC ATTATGGGAA ACTAAACTAA TCTCATCTGT 181 GTGTTGCAGT ACTATTGAAT CGTTGTAGTA TCTACCTGGA GGGCATTCCA TGAATTAGTG 241 AGATAACAGA GTTGGGTAAC TAGAGAGAAT AATAGACGTA TGCATGATTA CTACACAACG 301 GATGTCGCAC TCTTTCCTTA GTTAAAACTA TCATCCAATC ACAAGATGCG GGCTGGAAAG 361 ACTTGCTCCC GAAGGATAAT CTTCTGCTTC TATCTCCCTT CCTCATATGG TTTCGCAGGG 421 CTCATGCCCC TTCTTCCTTC GAACTGCCCG ATGAGGAAGT CCTTAGCCTA TCAAAGAATT 481 CGGGACCATC ATCGATTTTT AGAGCCTTAC CTGATCGCAA TCAGGATTTC ACTACTCATA 541 TAAATACATC GCTCAAAGCT CCAACTTTGC TTGTTCATAC AATTCTTGAT ATTCACACAA 601 TTGTTCACGT GGCCCAGCCG GCCGTCTCGG ATCGGTACCT CGAGCCGCGG CGGCCGCCAG 661 CTTGGGCCCA ATTGGGGTGG TAAGCCTATC CCTAACCCTC TCCTCGGTCT CGATTCTACG 721 GGTGTCGACC ATCATCATCA TCATCATTGA GTTTGTAGCC TTAGACATGA CTGTTCCTCA 781 GTTCAAGTTG GGCACTTACG AGAAGACCGG TCTTGCTAGA TTCTAATCAA GAGGATGTCA 841 GAATGCCATT TGCCTGAGAG ATGCAGGCTT CATTTTTGAT ACTTTTTTAT TTGTAACCTA 901 TATAGTATAG GATTTTTTTT GTCATTTTGT TTCTTCTCGT ACGAGCTTGC TCCTGATCAG 961 CCTATCTCGC AGCTGATGAA TATCTTGTGG TAGGGGTTTG GGAAAATCAT TCGAGTTTGA 1021 TGTTTTTCTT GGTATTTCCC ACTCCTCTTC AGAGTACAGA AGATTAAGTG AGACCTTCGT 1081 TTGTGCGGAT CCGGCGCGCC GATCCGCCTG ATGCGGTATT TTCTCCTTAC GCATCTGTGC 1141 GGTATTTCAC ACCGCATATA TGGTGCACTC TCAGTACAAT CTGCTCTGAT GCCGCATAGT 1201 TAAGCCAGCC CCGACACCCG CCAACACCCG CTGACGCGCC CTGACGGGCT TGTCTGCTCC 1261 CGGCATCCGC TTACAGACAA GCTGTGACCG TCTCCGGGAG CTGCATGTGT CAGAGGTTTT 1321 CACCGTCATC ACCGAAACGC GCGAGACGAA AGGGCCTCGT GATACGCCTA TTTTTATAGG 1381 TTAATGTCAT GATAATAATG GTTTCTTAGA CGTCAGGTGG CACTTTTCGG GGAAATGTGC 1441 GCGGAACCCC TATTTGTTTA TTTTTCTAAA TACATTCAAA TATGTATCCG CTCATGAGAC 1501 AATAACCCTG ATAAATGCTT CAATAATATT GAAAAAGGAA GAGTATGAGT ATTCAACATT 1561 TCCGTGTCGC CCTTATTCCC TTTTTTGCGG CATTTTGCCT TCCTGTTTTT GCTCACCCAG 1621 AAACGCTGGT GAAAGTAAAA GATGCTGAAG ATCAGTTGGG TGCACGAGTG GGTTACATCG 1681 AACTGGATCT CAACAGCGGT AAGATCCTTG AGAGTTTTCG CCCCGAAGAA CGTTTTCCAA 1741 TGATGAGCAC TTTTAAAGTT CTGCTATGTG GCGCGGTATT ATCCCGTATT GACGCCGGGC 1801 AAGAGCAACT CGGTCGCCGC ATACACTATT CTCAGAATGA CTTGGTTGAG TACTCACCAG 1861 TCACAGAAAA GCATCTTACG GATGGCATGA CAGTAAGAGA ATTATGCAGT GCTGCCATAA 1921 CCATGAGTGA TAACACTGCG GCCAACTTAC TTCTGACAAC GATCGGAGGA CCGAAGGAGC 1981 TAACCGCTTT TTTGCACAAC ATGGGGGATC ATGTAACTCG CCTTGATCGT TGGGAACCGG 2041 AGCTGAATGA AGCCATACCA AACGACGAGC GTGACACCAC GATGCCTGTA GCAATGGCAA 2101 CAACGTTGCG CAAACTATTA ACTGGCGAAC TACTTACTCT AGCTTCCCGG CAACAATTAA 2161 TAGACTGGAT GGAGGCGGAT AAAGTTGCAG GACCACTTCT GCGCTCGGCC CTTCCGGCTG 2221 GCTGGTTTAT TGCTGATAAA TCTGGAGCCG GTGAGCGTGG GTCTCGCGGT ATCATTGCAG 2281 CACTGGGGCC AGATGGTAAG CCCTCCCGTA TCGTAGTTAT CTACACGACG GGGAGTCAGG 2341 CAACTATGGA TGAACGAAAT AGACAGATCG CTGAGATAGG TGCCTCACTG ATTAAGCATT 2401 GGTAACTGTC AGACCAAGTT TACTCATATA TACTTTAGAT TGATTTAAAA CTTCATTTTT 2461 AATTTAAAAG GATCTAGGTG AAGATCCTTT TTGATAATCT CATGACCAAA ATCCCTTAAC 2521 GTGAGTTTTC GTTCCACTGA GCGTCAGACC CCGTAGAAAA GATCAAAGGA TCTTCTTGAG 2581 ATCCTTTTTT TCTGCGCGTA ATCTGCTGCT TGCAAACAAA AAAACCACCG CTACCAGCGG 2641 TGGTTTGTTT GCCGGATCAA GAGCTACCAA CTCTTTTTCC GAAGGTAACT GGCTTCAGCA 2701 GAGCGCAGAT ACCAAATACT GTTCTTCTAG TGTAGCCGTA GTTAGGCCAC CACTTCAAGA 2761 ACTCTGTAGC ACCGCCTACA TACCTCGCTC TGCTAATCCT GTTACCAGTG GCTGCTGCCA 2821 GTGGCGATAA GTCGTGTCTT ACCGGGTTGG ACTCAAGACG ATAGTTACCG GATAAGGCGC 2881 AGCGGTCGGG CTGAACGGGG GGTTCGTGCA CACAGCCCAG CTTGGAGCGA ACGACCTACA 2941 CCGAACTGAG ATACCTACAG CGTGAGCTAT GAGAAAGCGC CACGCTTCCC GAAGGGAGAA 3001 AGGCGGACAG GTATCCGGTA AGCGGCAGGG TCGGAACAGG AGAGCGCACG AGGGAGCTTC 3061 CAGGGGGAAA CGCCTGGTAT CTTTATAGTC CTGTCGGGTT TCGCCACCTC TGACTTGAGC 3121 GTCGATTTTT GTGATGCTCG TCAGGGGGGC GGAGCCTATG GAAAAACGCC AGCAACGCGG 3181 CCTTTTTACG GTTCCTGGCC TTTTGCTGGC CTTTTGCTCA CACACCATAG CTTCAAAATG 3241 TTTCTACTCC TTTTTTACTC TTCCAGATTT TCTCGGACTC CGCGCATCGC CGTACCACTT 3301 CAAAACACCC AAGCACAGCA TACTAAATTT CCCCTCTTTC TTCCTCTAGG GTGTCGTTAA 3361 TTACCCGTAC TAAAGGTTTG GAAAAGAAAA AAGAGACCGC CTCGTTTCTT TTTCTTCGTC 3421 GAAAAAGGCA ATAAAAATTT TTATCACGTT TCTTTTTCTT GAAAATTTTT TTTTTTGATT 3481 TTTTTCTCTT TCGATGACCT CCCATTGATA TTTAAGTTAA TAAACGGTCT TCAATTTCTC 3541 AAGTTTCAGT TTCATTTTTC TTGTTCTATT ACAACTTTTT TTACTTCTTG CTCATTAGAA 3601 AGAAAGCATA GCAATCTAAT CTAAGGGCGG CGTTGACAAT TAATCATCGG CATAGTATAT 3661 CGGCATAGTA TAATACGACA AGGTGAGGAA CTAAACCATG GCCAAGTTGA CCAGTGCCGT 3721 TCCGGTGCTC ACCGCGCGCG ACGTCGCCGG AGCGGTCGAG TTCTGGACCG ACCGGCTCGG 3781 GTTCTCCCGG GACTTCGTGG AGGACGACTT CGCCGGTGTG GTCCGGGACG ACGTGACCCT 3841 GTTCATCAGC GCGGTCCAGG ACCAGGTGGT GCCGGACAAC ACCCTGGCCT GGGTGTGGGT 3901 GCGCGGCCTG GACGAGCTGT ACGCCGAGTG GTCGGAGGTC GTGTCCACGA ACTTCCGGGA 3961 CGCCTCCGGG CCGGCCATGA CCGAGATCGG CGAGCAGCCG TGGGGGCAGG AGTTCGCCCT 4021 GCGCGACCCG GCCGGCAACT GCGTGCACTT CGTGGCCGAG GAGCAGGACT GACACGTCCG 4081 ACGCGGCCCG ACGGGTCCGA GGCCTCGGAG ATCCGTCCCC CTTTTCCTTT GTCGATATCA 4141 TGTAATTAGT TATGTCACGC TTACATTCAC GCCCTCCCCC CACATCCGCT CTAACCGAAA 4201 AGGAAGGAGT TAGACAACCT GAAGTCTAGG TCCCTATTTA TTTTTTTATA GTTATGTTAG 4261 TATTAAGAAC GTTATTTATA TTTCAAATTT TTCTTTTTTT TCTGTACAGA CGCGTGTACG 4321 CATGTAACAT TATACTGAAA ACCTTGCTTG AGAAGGTTTT GGGACGCTCG AAGGCTTTAA 4381 TTTGCAAGCT GAATTGCGGC CGCAGATCT //9pFLDðÿÿÿ"Times New RomanÍÍÍÍØp¤<ÿCÍÍÍÍ9§ÐŽV5óÿÿÿ"Times New RomanèÍÍÍÍØp¤<†CÍÍÍÍ9ÚëŽ6xHisóÿÿÿ"Times New RomanèÍÍÍÍØp¤<CɆÍÍÍÍ9AUŒ 3_AOX1_prióÿÿÿ"Times New RomanèÍÍÍÍØp¤<†ÉÍÍÍÍ9$Ž pGEX_3_prióÿÿÿ"Times New RomanèÍÍÍÍØp¤<ÉCÍÍÍÍ9ÃߎAmpR_proóÿÿÿ"Times New RomanèÍÍÍÍØp¤<†CÍÍÍÍ9 e ŽORF 3óÿÿÿ"Times New RomanèÍÍÍÍØp¤<CɆÍÍÍÍ9 e Ž Ampicillinóÿÿÿ"Times New RomanèÍÍÍÍØp¤<†ÉÍÍÍÍ9 k Ž pBR322_originóÿÿÿ"Times New RomanèÍÍÍÍØp¤<ÉCÍÍÍÍ9råŽbleoóÿÿÿ"Times New RomanèÍÍÍÍØp¤<†CÍÍÍÍ9rèŽsh_bleóÿÿÿ"Times New RomanèÍÍÍÍØp¤<ÉCÍÍÍÍ9 &Ž CYC1_termóÿÿÿ"Times New RomanèÍÍÍÍØp¤<CɆÍÍÍÍ9:LŒCYC1_prióÿÿÿ"Times New RomanèÍÍÍÍØp¤<†ÉÍÍÍÍ9:LŒpYESTrp_rev_prióÿÿÿ"Times New Romanè* ÍÍÍÍØp¤<ÍÍÍÍFspIóÿÿÿ"Times New Roman=9Þ9ßCØOšOØ^ ÍÍÍÍØp¤<ÍÍÍÍBglIIóÿÿÿ"Times New 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