Sequence of pFastBac

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Sequence of pFastBac_Dual

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Sequence of pFastBac Dual : 5238 BP; 1294 A; 1282 C; 1259 G; 1403 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 ttctctgtca cagaatgaaa atttttctgt catctcttcg ttattaatgt ttgtaattga 60

1 aagagacagt gtcttacttt taaaaagaca gtagagaagc aataattaca aacattaact 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 ctgaatatca acgcttattt gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc 120

61 gacttatagt tgcgaataaa cgtcggactt accgcttacc ctgcgcggga catcgccgcg 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct 180

121 taattcgcgc cgcccacacc accaatgcgc gtcgcactgg cgatgtgaac ggtcgcggga 180

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg 240

181 tcgcgggcga ggaaagcgaa agaagggaag gaaagagcgg tgcaagcggc cgaaaggggc 240

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga 300

241 agttcgagat ttagcccccg agggaaatcc caaggctaaa tcacgaaatg ccgtggagct 300

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt 360

301 ggggtttttt gaactaatcc cactaccaag tgcatcaccc ggtagcggga ctatctgcca 360

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg 420

361 aaaagcggga aactgcaacc tcaggtgcaa gaaattatca cctgagaaca aggtttgacc 420

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 aacaacactc aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc 480

421 ttgttgtgag ttgggataga gccagataag aaaactaaat attccctaaa acggctaaag 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat 540

481 ccggataacc aattttttac tcgactaaat tgtttttaaa ttgcgcttaa aattgtttta 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 attaacgttt acaatttcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 600

541 taattgcaaa tgttaaagtc caccgtgaaa agccccttta cacgcgcctt ggggataaac 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 660

601 aaataaaaag atttatgtaa gtttatacat aggcgagtac tctgttattg ggactattta 660

.......... .......... ........ AmpR_promoter ........ ..........

.......... .......... .......... .......... .......... ..........

661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat 720

661 cgaagttatt ataacttttt ccttctcata ctcataagtt gtaaaggcac agcgggaata 720

.......... .......... .......... .... Orf frame 2 Ampicillin ....

.......... .......... .......... .......... .......... ..........

721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt 780

721 agggaaaaaa cgccgtaaaa cggaaggaca aaaacgagtg ggtctttgcg accactttca 780

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag 840

781 ttttctacga cttctagtca acccacgtgc tcacccaatg tagcttgacc tagagttgtc 840

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa 900

841 gccattctag gaactctcaa aagcggggct tcttgcaaaa ggttactact cgtgaaaatt 900

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg 960

901 tcaagacgat acaccgcgcc ataatagggc ataactgcgg cccgttctcg ttgagccagc 960

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct 1020

961 ggcgtatgtg ataagagtct tactgaacca actcatgagt ggtcagtgtc ttttcgtaga 1020

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac 1080

1021 atgcctaccg tactgtcatt ctcttaatac gtcacgacgg tattggtact cactattgtg 1080

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca 1140

1081 acgccggttg aatgaagact gttgctagcc tcctggcttc ctcgattggc gaaaaaacgt 1140

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat 1200

1141 gttgtacccc ctagtacatt gagcggaact agcaaccctt ggcctcgact tacttcggta 1200

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... FspI(1253)

1201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact 1260

1201 tggtttgctg ctcgcactgt ggtgctacgg acatcgttac cgttgttgca acgcgtttga 1260

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc 1320

1261 taattgaccg cttgatgaat gagatcgaag ggccgttgtt aattatctga cctacctccg 1320

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga 1380

1321 cctatttcaa cgtcctggtg aagacgcgag ccgggaaggc cgaccgacca aataacgact 1380

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg 1440

1381 atttagacct cggccactcg cacccagagc gccatagtaa cgtcgtgacc ccggtctacc 1440

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg 1500

1441 attcgggagg gcatagcatc aatagatgtg ctgcccctca gtccgttgat acctacttgc 1500

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca 1560

1501 tttatctgtc tagcgactct atccacggag tgactaattc gtaaccattg acagtctggt 1560

.......... .... Orf frame 2 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta 1620

1561 tcaaatgagt atatatgaaa tctaactaaa ttttgaagta aaaattaaat tttcctagat 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca 1680

1621 ccacttctag gaaaaactat tagagtactg gttttaggga attgcactca aaagcaaggt 1680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg 1740

1681 gactcgcagt ctggggcatc ttttctagtt tcctagaaga actctaggaa aaaaagacgc 1740

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

1741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga 1800

1741 gcattagacg acgaacgttt gtttttttgg tggcgatggt cgccaccaaa caaacggcct 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa 1860

1801 agttctcgat ggttgagaaa aaggcttcca ttgaccgaag tcgtctcgcg tctatggttt 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc 1920

1861 atgacaggaa gatcacatcg gcatcaatcc ggtggtgaag ttcttgagac atcgtggcgg 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg 1980

1921 atgtatggag cgagacgatt aggacaatgg tcaccgacga cggtcaccgc tattcagcac 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac 2040

1981 agaatggccc aacctgagtt ctgctatcaa tggcctattc cgcgtcgcca gcccgacttg 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct 2100

2041 ccccccaagc acgtgtgtcg ggtcgaacct cgcttgctgg atgtggcttg actctatgga 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc 2160

2101 tgtcgcactc gtaactcttt cgcggtgcga agggcttccc tctttccgcc tgtccatagg 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg 2220

2161 ccattcgccg tcccagcctt gtcctctcgc gtgctccctc gaaggtcccc ctttgcggac 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg 2280

2221 catagaaata tcaggacagc ccaaagcggt ggagactgaa ctcgcagcta aaaacactac 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct 2340

2281 gagcagtccc cccgcctcgg ataccttttt gcggtcgttg cgccggaaaa atgccaagga 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga 2400

2341 ccggaaaacg accggaaaac gagtgtacaa gaaaggacgc aataggggac taagacacct 2400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg 2460

2401 attggcataa tggcggaaac tcactcgact atggcgagcg gcgtcggctt gctggctcgc 2460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cagcgagtca gtgagcgagg aagcggaaga gcgcctgatg cggtattttc tccttacgca 2520

2461 gtcgctcagt cactcgctcc ttcgccttct cgcggactac gccataaaag aggaatgcgt 2520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 tctgtgcggt atttcacacc gcagaccagc cgcgtaacct ggcaaaatcg gttacggttg 2580

2521 agacacgcca taaagtgtgg cgtctggtcg gcgcattgga ccgttttagc caatgccaac 2580

.......... .......... .......... .......... Tn7_att ..........

.......... .......... .......... .......... .......... ..........

2581 agtaataaat ggatgccctg cgtaagcggg tgtgggcgga caataaagtc ttaaactgaa 2640

2581 tcattattta cctacgggac gcattcgccc acacccgcct gttatttcag aatttgactt 2640

.......... Tn7_att .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 caaaatagat ctaaactatg acaataaagt cttaaactag acagaatagt tgtaaactga 2700

2641 gttttatcta gatttgatac tgttatttca gaatttgatc tgtcttatca acatttgact 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aatcagtcca gttatgctgt gaaaaagcat actggacttt tgttatggct aaagcaaact 2760

2701 ttagtcaggt caatacgaca ctttttcgta tgacctgaaa acaataccga tttcgtttga 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cttcattttc tgaagtgcaa attgcccgtc gtattaaaga ggggcgtggc caagggcatg 2820

2761 gaagtaaaag acttcacgtt taacgggcag cataatttct ccccgcaccg gttcccgtac 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gtaaagacta tattcgcggc gttgtgacaa tttaccgaac aactccgcgg ccgggaagcc 2880

2821 catttctgat ataagcgccg caacactgtt aaatggcttg ttgaggcgcc ggcccttcgg 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... ......... EcoRV(2925) .........

2881 gatctcggct tgaacgaatt gttaggtggc ggtacttggg tcgatatcaa agtgcatcac 2940

2881 ctagagccga acttgcttaa caatccaccg ccatgaaccc agctatagtt tcacgtagtg 2940

.......... .......... .......... .......... gent ..........

.......... .......... .......... .......... .......... ..........

2941 ttcttcccgt atgcccaact ttgtatagag agccactgcg ggatcgtcac cgtaatctgc 3000

2941 aagaagggca tacgggttga aacatatctc tcggtgacgc cctagcagtg gcattagacg 3000

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ttgcacgtag atcacataag caccaagcgc gttggcctca tgcttgagga gattgatgag 3060

3001 aacgtgcatc tagtgtattc gtggttcgcg caaccggagt acgaactcct ctaactactc 3060

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgcggtggca atgccctgcc tccggtgctc gccggagact gcgagatcat agatatagat 3120

3061 gcgccaccgt tacgggacgg aggccacgag cggcctctga cgctctagta tctatatcta 3120

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctcactacgc ggctgctcaa acctgggcag aacgtaagcc gcgagagcgc caacaaccgc 3180

3121 gagtgatgcg ccgacgagtt tggacccgtc ttgcattcgg cgctctcgcg gttgttggcg 3180

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 ttcttggtcg aaggcagcaa gcgcgatgaa tgtcttacta cggagcaagt tcccgaggta 3240

3181 aagaaccagc ttccgtcgtt cgcgctactt acagaatgat gcctcgttca agggctccat 3240

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 atcggagtcc ggctgatgtt gggagtaggt ggctacgtct ccgaactcac gaccgaaaag 3300

3241 tagcctcagg ccgactacaa ccctcatcca ccgatgcaga ggcttgagtg ctggcttttc 3300

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 atcaagagca gcccgcatgg atttgacttg gtcagggccg agcctacatg tgcgaatgat 3360

3301 tagttctcgt cgggcgtacc taaactgaac cagtcccggc tcggatgtac acgcttacta 3360

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 gcccatactt gagccaccta actttgtttt agggcgactg ccctgctgcg taacatcgtt 3420

3361 cgggtatgaa ctcggtggat tgaaacaaaa tcccgctgac gggacgacgc attgtagcaa 3420

.......... .......... gent .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 gctgctgcgt aacatcgttg ctgctccata acatcaaaca tcgacccacg gcgtaacgcg 3480

3421 cgacgacgca ttgtagcaac gacgaggtat tgtagtttgt agctgggtgc cgcattgcgc 3480

gent .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 cttgctgctt ggatgcccga ggcatagact gtacaaaaaa acagtcataa caagccatga 3540

3481 gaacgacgaa cctacgggct ccgtatctga catgtttttt tgtcagtatt gttcggtact 3540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 aaaccgccac tgcgccgtta ccaccgctgc gttcggtcaa ggttctggac cagttgcgtg 3600

3541 tttggcggtg acgcggcaat ggtggcgacg caagccagtt ccaagacctg gtcaacgcac 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 agcgcatacg ctacttgcat tacagtttac gaaccgaaca ggcttatgtc aactgggttc 3660

3601 tcgcgtatgc gatgaacgta atgtcaaatg cttggcttgt ccgaatacag ttgacccaag 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gtgccttcat ccgtttccac ggtgtgcgtc acccggcaac cttgggcagc agcgaagtcg 3720

3661 cacggaagta ggcaaaggtg ccacacgcag tgggccgttg gaacccgtcg tcgcttcagc 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 aggcatttct gtcctggctg gcgaacgagc gcaaggtttc ggtctccacg catcgtcagg 3780

3721 tccgtaaaga caggaccgac cgcttgctcg cgttccaaag ccagaggtgc gtagcagtcc 3780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cattggcggc cttgctgttc ttctacggca aggtgctgtg cacggatctg ccctggcttc 3840

3781 gtaaccgccg gaacgacaag aagatgccgt tccacgacac gtgcctagac gggaccgaag 3840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 aggagatcgg tagacctcgg ccgtcgcggc gcttgccggt ggtgctgacc ccggatgaag 3900

3841 tcctctagcc atctggagcc ggcagcgccg cgaacggcca ccacgactgg ggcctacttc 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 tggttcgcat cctcggtttt ctggaaggcg agcatcgttt gttcgcccag gactctagct 3960

3901 accaagcgta ggagccaaaa gaccttccgc tcgtagcaaa caagcgggtc ctgagatcga 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 atagttctag tggttggcct acgtacccgt agtggctatg gcagggcttg ccgccccgac 4020

3961 tatcaagatc accaaccgga tgcatgggca tcaccgatac cgtcccgaac ggcggggctg 4020

.......... .......... .......... ....... TK_PA_terminator .......

.......... .......... .......... .......... .......... ..........

4021 gttggctgcg agccctgggc cttcacccga acttgggggt tggggtgggg aaaaggaaga 4080

4021 caaccgacgc tcgggacccg gaagtgggct tgaaccccca accccacccc ttttccttct 4080

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aacgcgggcg tattggtccc aatggggtct cggtggggta tcgacagagt gccagccctg 4140

4081 ttgcgcccgc ataaccaggg ttaccccaga gccaccccat agctgtctca cggtcgggac 4140

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ggaccgaacc ccgcgtttat gaacaaacga cccaacaccc gtgcgtttta ttctgtcttt 4200

4141 cctggcttgg ggcgcaaata cttgtttgct gggttgtggg cacgcaaaat aagacagaaa 4200

.......... ....... TK_PA_terminator ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 ttattgccgt catagcgcgg gttccttccg gtattgtctc cttccgtgtt tcagttagcc 4260

4201 aataacggca gtatcgcgcc caaggaaggc cataacagag gaaggcacaa agtcaatcgg 4260

.......... ....... TK_PA_terminator ....... .......... ..........

.......... KpnI(4278) .... PvuII(4295),NheI(4298) NcoI(4305) ....

4261 tcccccatct cccggtaccg catgctatgc atcagctgct agcaccatgg ctcgagatcc 4320

4261 agggggtaga gggccatggc gtacgatacg tagtcgacga tcgtggtacc gagctctagg 4320

.......... .......... .......... .......... .......... ..........

SmaI(4321) .......... .......... .......... .......... ..........

4321 cgggtgatca agtcttcgtc gagtgattgt aaataaaatg taatttacag tatagtattt 4380

4321 gcccactagt tcagaagcag ctcactaaca tttattttac attaaatgtc atatcataaa 4380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 taattaatat acaaatgatt tgataataat tcttatttaa ctataatata ttgtgttggg 4440

4381 attaattata tgtttactaa actattatta agaataaatt gatattatat aacacaaccc 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 ttgaattaaa ggtccgtata ctccggaata ttaatagatc atggagataa ttaaaatgat 4500

4441 aacttaattt ccaggcatat gaggccttat aattatctag tacctctatt aattttacta 4500

.......... .......... .......... ......... PH_promoter .........

.......... .......... .......... .......... .......... ..........

4501 aaccatctcg caaataaata agtattttac tgttttcgta acagttttgt aataaaaaaa 4560

4501 ttggtagagc gtttatttat tcataaaatg acaaaagcat tgtcaaaaca ttattttttt 4560

polyhedrin_fwd_primer PH_promoter ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 cctataaata ttccggatta ttcataccgt cccaccatcg ggcgcggatc ccggtccgaa 4620

4561 ggatatttat aaggcctaat aagtatggca gggtggtagc ccgcgcctag ggccaggctt 4620

.......... .......... .......... .......... .......... ..........

EcoRI StuI SalI) SacI(4654),SpeI(4656) NotI(4664)

4621 gcgcgcggaa ttcaaaggcc tacgtcgacg agctcactag tcgcggccgc tttcgaatct 4680

4621 cgcgcgcctt aagtttccgg atgcagctgc tcgagtgatc agcgccggcg aaagcttaga 4680

.......... .......... .......... .......... .......... ..........

PstI(4690) HindIII(4699) ...... .......... .......... ..........

4681 agagcctgca gtctcgacaa gcttgtcgag aagtactaga ggatcataat cagccatacc 4740

4681 tctcggacgt cagagctgtt cgaacagctc ttcatgatct cctagtatta gtcggtatgg 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa 4800

4741 tgtaaacatc tccaaaatga acgaaatttt ttggagggtg tggaggggga cttggacttt 4800

.......... .......... .......... .......... .......... ..........

.......... .......... HpaI(4827) .......... .......... ..........

4801 cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa 4860

4801 gtattttact tacgttaaca acaacaattg aacaaataac gtcgaatatt accaatgttt 4860

.......... .......... .......... ...... SV40_PA_terminator ......

.......... .......... .......... .......... .......... ..........

4861 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt 4920

4861 atttcgttat cgtagtgttt aaagtgttta tttcgtaaaa aaagtgacgt aagatcaaca 4920

.......... ...... SV40_PA_terminator ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgatcact gcttgagcct 4980

4921 ccaaacaggt ttgagtagtt acatagaata gtacagacct agactagtga cgaactcgga 4980

EBV_rev_primer .. SV40_PA_terminator ...... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aggagatccg aaccagataa gtgaaatcta gttccaaact attttgtcat ttttaatttt 5040

4981 tcctctaggc ttggtctatt cactttagat caaggtttga taaaacagta aaaattaaaa 5040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 cgtattagct tacgacgcta cacccagttc ccatctattt tgtcactctt ccctaaataa 5100

5041 gcataatcga atgctgcgat gtgggtcaag ggtagataaa acagtgagaa gggatttatt 5100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tccttaaaaa ctccatttcc acccctccca gttcccaact attttgtccg cccacagcgg 5160

5101 aggaattttt gaggtaaagg tggggagggt caagggttga taaaacaggc gggtgtcgcc 5160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 ggcatttttc ttcctgttat gtttttaatc aaacatcctg ccaactccat gtgacaaacc 5220

5161 ccgtaaaaag aaggacaata caaaaattag tttgtaggac ggttgaggta cactgtttgg 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 gtcatcttcg gctacttt.. .......... .......... .......... .......... 5238

5221 cagtagaagc cgatgaaa.. .......... .......... .......... .......... 5238

.......... .......... .......... .......... .......... ..........