v5LOCUS pGAD GH 8009 bp DNA SYN 29-Aug-2007 DEFINITION pGAD GH ACCESSION KEYWORDS SOURCE ORGANISM other sequences; artificial sequences; vectors. FEATURES Location/Qualifiers source 1..8009 /organism="pGAD GH" /mol_type="other DNA" promoter 1..397 /label="yADH1_promoter" misc_feature 422..478 /label="SV40_NLS" misc_feature 413..820 /label="Gal4_AD" misc_feature 482..820 /label="Gal4_AD" misc_feature 746..763 /label="Gal4_AD_primer" misc_feature complement(874..890) /label="pBluescriptKS_primer" promoter complement(915..933) /label="T7_promoter" misc_feature complement(940..956) /label="M13_forward20_primer" misc_feature complement(949..971) /label="M13_pUC_fwd_primer" misc_feature complement(1238..1261) /label="pJG4_5_rev_primer" terminator 1222..1548 /label="yADH2_terminator" misc_feature complement(1414..1438) /label="pHybLex_rev_primer" terminator 1359..1538 /label="yADH1_terminator" CDS complement(1663..2757) /label="LEU2(variant)" /gene="LEU2(variant)" /note="ORF frame 1" /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENH LIGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQL YANLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSE QYTVPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLK VQHQLIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPD KNTAFGLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLD AGIRTGDLGGSNSTTEVGDAVAEEVKKILA*" gene complement(1663..2757) /label="LEU2(variant)" /gene="LEU2(variant)" misc_feature complement(3200..3218) /label="M13_reverse_primer" misc_feature complement(3217..3239) /label="M13_pUC_rev_primer" promoter complement(3253..3282) /label="lac_promoter" misc_feature complement(3373..3406) /label="loxP" misc_feature complement(3484..3517) /label="loxP" rep_origin complement(3742..4362) /label="pBR322_origin" CDS complement(4517..5377) /label="Ampicillin" /gene="Ampicillin" /note="ORF frame 2" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW*" gene complement(4517..5377) /label="Ampicillin" /gene="Ampicillin" promoter complement(5419..5447) /label="AmpR_promoter" misc_feature complement(5606..5628) /label="pGEX_3_primer" rep_origin 5764..6928 /label="2micron2_origin" ORIGIN 1 CAACTTCTTT TCTTTTTTTT TCTTTTCTCT CTCCCCCGTT GTTGTCTCAC CATATCCGCA 61 ATGACAAAAA AAATGATGGA AGACACTAAA GGAAAAAATT AACGACAAAG ACAGCACCAA 121 CAGATGTCGT TGTTCCAGAG CTGATGAGGG GTATCTCGAA CACACGAAAC TTTTTCCTTC 181 CTTCATTCAC GCACACTACT CTCTAATGAG CAACGGTATA CGGCCTTCCT TCCAGTTACT 241 TGAATTTGAA ATAAAAAAAA GTTTGCCGCT TTGCTATCAA GTATAAATAG ACCTGCAATT 301 ATTAATCTTT TGTTTCCTCG TCATTGTTCT CGTTCCCTTT CTTCCTTGTT TCTTTTTCTG 361 CACAATATTT CAAGCTATAC CAAGCATACA ATCAACTCCA AGCTTTGCAA AGATGGATAA 421 AGCGGAATTA ATTCCCGAGC CTCCAAAAAA GAAGAGAAAG GTCGAATTGG GTACCGCCGC 481 CAATTTTAAT CAAAGTGGGA ATATTGCTGA TAGCTCATTG TCCTTCACTT TCACTAACAG 541 TAGCAACGGT CCGAACCTCA TAACAACTCA AACAAATTCT CAAGCGCTTT CACAACCAAT 601 TGCCTCCTCT AACGTTCATG ATAACTTCAT GAATAATGAA ATCACGGCTA GTAAAATTGA 661 TGATGGTAAT AATTCAAAAC CACTGTCACC TGGTTGGACG GACCAAACTG CGTATAACGC 721 GTTTGGAATC ACTACAGGGA TGTTTAATAC CACTACAATG GATGATGTAT ATAACTATCT 781 ATTCGATGAT GAAGATACCC CACCAAACCC AAAAAAAGAG ATCCTAGAAC TAGTGGATCC 841 CCCGGGCTGC AGGAATTCGA TATCAAGCTT ATCGATACCG TCGACCTCGA GGGGGGGCCC 901 GGTACCCAAT TCGCCCTATA GTGAGTCGTA TTACAATTCA CTGGCCGTCG TTTTACAACG 961 TCGTGACTGG GAAAACCCTG ATCTATGAAT CGTAGATACT GAAAAACCCC GCAAGTTCAC 1021 TTCAACTGTG CATCGTGCAC CATCTCAATT TCTTTCATTT ATACATCGTT TTGCCTTCTT 1081 TTATGTAACT ATACTCCTCT AAGTTTCAAT CTTGGCCATG TAACCTCTGA TCTATAGAAT 1141 TTTTTAAATG ACTAGAATTA ATGCCCATCT TTTTTTTGGA CCTAAATTCT TCATGAAAAT 1201 ATATTACGAG GGCTTATTCA GAAGCTTTGG ACTTCTTCGC CAGAGGTTTG GTCAAGTCTC 1261 CAATCAAGGT TGTCGGCTTG TCTACCTTGC CAGAAATTTA CGAAAAGATG GAAAAGGGTC 1321 AAATCGTTGG TAGATACGTT GTTGACACTT CTAAATAAGC GAATTTCTTA TGATTTATGA 1381 TTTTTATTAT TAAATAAGTT ATAAAAAAAA TAAGTGTATA CAAATTTTAA AGTGACTCTT 1441 AGGTTTTAAA ACGAAAATTC TTATTCTTGA GTAACTCTTT CCTGTAGGTC AGGTTGCTTT 1501 CTCAGGTATA GCATGAGGTC GCTCTTATTG ACCACACCTC TACCGGCCGG TCGAAATTCC 1561 CCTACCCTAT GAACATATTC CATTTTGTAA TTTCGTGTCG TTTCTATTAT GAATTTCATT 1621 TATAAAGTTT ATGTACAAAT ATCATAAAAA AAGAGAATCT TTTTAAGCAA GGATTTTCTT 1681 AACTTCTTCG GCGACAGCAT CACCGACTTC GGTGGTACTG TTGGAACCAC CTAAATCACC 1741 AGTTCTGATA CCTGCATCCA AAACCTTTTT AACTGCATCT TCAATGGCCT TACCTTCTTC 1801 AGGCAAGTTC AATGACAATT TCAACATCAT TGCAGCAGAC AAGATAGTGG CGATAGGGTC 1861 AACCTTATTC TTTGGCAAAT CTGGAGCAGA ACCGTGGCAT GGTTCGTACA AACCAAATGC 1921 GGTGTTCTTG TCTGGCAAAG AGGCCAAGGA CGCAGATGGC AACAAACCCA AGGAACCTGG 1981 GATAACGGAG GCTTCATCGG AGATGATATC ACCAAACATG TTGCTGGTGA TTATAATACC 2041 ATTTAGGTGG GTTGGGTTCT TAACTAGGAT CATGGCGGCA GAATCAATCA ATTGATGTTG 2101 AACCTTCAAT GTAGGAAATT CGTTCTTGAT GGTTTCCTCC ACAGTTTTTC TCCATAATCT 2161 TGAAGAGGCC AAAACATTAG CTTTATCCAA GGACCAAATA GGCAATGGTG GCTCATGTTG 2221 TAGGGCCATG AAAGCGGCCA TTCTTGTGAT TCTTTGCACT TCTGGAACGG TGTATTGTTC 2281 ACTATCCCAA GCGACACCAT CACCATCGTC TTCCTTTCTC TTACCAAAGT AAATACCTCC 2341 CACTAATTCT CTGACAACAA CGAAGTCAGT ACCTTTAGCA AATTGTGGCT TGATTGGAGA 2401 TAAGTCTAAA AGAGAGTCGG ATGCAAAGTT ACATGGTCTT AAGTTGGCGT ACAATTGAAG 2461 TTCTTTACGG ATTTTTAGTA AACCTTGTTC AGGTCTAACA CTACCTGTAC CCCATTTAGG 2521 ACCACCCACA GCACCTAACA AAACGGCATC AACCTTCTTG GAGGCTTCCA GCGCCTCATC 2581 TGGAAGTGGG ACACCTGTAG CATCGATAGC AGCACCACCA ATTAAATGAT TTTCGAAATC 2641 GAACTTGACA TTGGAACGAA CATCAGAAAT AGCTTTAAGA ACCTTAATGG CTTCGGCTGT 2701 GATTTCTTGA CCAACGTGGT CACCTGGCAA AACGACGATC TTCTTAGGGG CAGACATTAG 2761 AATGGTATAT CCTTGAAATA TATATATATA TTGCTGAAAT GTAAAAGGTA AGAAAAGTTA 2821 GAAAGTAAGA CGATTGCTAA CCACCTATTG GAAAAAACAA TAGGTCCTTA AATAATATTG 2881 TCAACTTCAA GTATTGTGAT GCAAGCATTT AGTCATGAAC GCTTCTCTAT TCTATATGAA 2941 AAGCCGGTTC CGGCCTCTCA CCTTTCCTTT TTCTCCCAAT TTTTCAGTTG AAAAAGGTAT 3001 ATGCGTCAGG CGACCTCTGA AATTAACAAA AAATTTCCAG TCATCGAATT TGATTCTGTG 3061 CGATAGCGCC CCTGTGTGTT CTCGTTATGT TGAGGAAAAA AATAATGGTT GCTAAGAGAT 3121 TCGAACTCTT GCATCTTACG ATACCTGAGT ATTCCCACAG TTGGGGATCT CGACTCTAGC 3181 TAGAGGATCA ATTCGTAATC ATGGTCATAG CTGTTTCCTG TGTGAAATTG TTATCCGCTC 3241 ACAATTCCAC ACAACATACG AGCCGGAAGC ATAAAGTGTA AAGCCTGGGG TGCCTAATGA 3301 GTGAGCTAAC TCACATTAAT TGCGTTGCGC TCACTGCCCG CTTTCCAGTC GGGAAACCTG 3361 TCGTGCCAGC TGATAACTTC GTATAATGTA TGCTATACGA AGTTATTAGG TCTGAAGAGG 3421 AGTTTACGTC CAGCCAAGCT AGCTTGGCTG CAGGTCGAGC GGCCGCGATC CGGAACCCTT 3481 AATATAACTT CGTATAATGT ATGCTATACG AAGTTATCAG CTGCATTAAT GAATCGGCCA 3541 ACGCGCGGGG AGAGGCGGTT TGCGTATTGG GCGCTCTTCC GCTTCCTCGC TCACTGACTC 3601 GCTGCGCTCG GTCGTTCGGC TGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 3661 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 3721 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 3781 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 3841 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 3901 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 3961 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 4021 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 4081 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 4141 TGTAGGCGGT GCTAACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGGA 4201 CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT 4261 CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA 4321 TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG 4381 CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT 4441 TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT 4501 AAACTTGGTC TGACAGTTAC CAATGCTTAA TCAGTGAGGC ACCTATCTCA GCGATCTGTC 4561 TATTTCGTTC ATCCATAGTT GCCTGACTCC CCGTCGTGTA GATAACTACG ATACGGGAGG 4621 GCTTACCATC TGGCCCCAGT GCTGCAATGA TACCGCGAGA CCCACGCTCA CCGGCTCCAG 4681 ATTTATCAGC AATAAACCAG CCAGCCGGAA GGGCCGAGCG CAGAAGTGGT CCTGCAACTT 4741 TATCCGCCTC CATCCAGTCT ATTAATTGTT GCCGGGAAGC TAGAGTAAGT AGTTCGCCAG 4801 TTAATAGTTT GCGCAACGTT GTTGCCATTG CTACAGGCAT CGTGGTGTCA CGCTCGTCGT 4861 TTGGTATGGC TTCATTCAGC TCCGGTTCCC AACGATCAAG GCGAGTTACA TGATCCCCCA 4921 TGTTGTGCAA AAAAGCGGTT AGCTCCTTCG GTCCTCCGAT CGTTGTCAGA AGTAAGTTGG 4981 CCGCAGTGTT ATCACTCATG GTTATGGCAG CACTGCATAA TTCTCTTACT GTCATGCCAT 5041 CCGTAAGATG CTTTTCTGTG ACTGGTGAGT ACTCAACCAA GTCATTCTGA GAATAGTGTA 5101 TGCGGCGACC GAGTTGCTCT TGCCCGGCGT CAATACGGGA TAATACCGCG CCACATAGCA 5161 GAACTTTAAA AGTGCTCATC ATTGGAAAAC GTTCTTCGGG GCGAAAACTC TCAAGGATCT 5221 TACCGCTGTT GAGATCCAGT TCGATGTAAC CCACTCGTGC ACCCAACTGA TCTTCAGCAT 5281 CTTTTACTTT CACCAGCGTT TCTGGGTGAG CAAAAACAGG AAGGCAAAAT GCCGCAAAAA 5341 AGGGAATAAG GGCGACACGG AAATGTTGAA TACTCATACT CTTCCTTTTT CAATATTATT 5401 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 5461 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GTCTAAGAAA 5521 CCATTATTAT CATGACATTA ACCTATAAAA ATAGGCGTAT CACGAGGCCC TTTCGTCTCG 5581 CGCGTTTCGG TGATGACGGT GAAAACCTCT GACACATGCA GCTCCCGGAG ACGGTCACAG 5641 CTTGTCTGTA AGCGGATGCC GGGAGCAGAC AAGCCCGTCA GGGCGCGTCA GCGGGTGTTG 5701 GCGGGTGTCG GGGCTGGCTT AACTATGCGG CATCAGAGCA GATTGTACTG AGAGTGCACC 5761 ATAACGCATT TAAGCATAAA CACGCACTAT GCCGTTCTTC TCATGTATAT ATATATACAG 5821 GCAACACGCA GATATAGGTG CGACGTGAAC AGTGAGCTGT ATGTGCGCAG CTCGCGTTGC 5881 ATTTTCGGAA GCGCTCGTTT TCGGAAACGC TTTGAAGTTC CTATTCCGAA GTTCCTATTC 5941 TCTAGCTAGA AAGTATAGGA ACTTCAGAGC GCTTTTGAAA ACCAAAAGCG CTCTGAAGAC 6001 GCACTTTCAA AAAACCAAAA ACGCACCGGA CTGTAACGAG CTACTAAAAT ATTGCGAATA 6061 CCGCTTCCAC AAACATTGCT CAAAAGTATC TCTTTGCTAT ATATCTCTGT GCTATATCCC 6121 TATATAACCT ACCCATCCAC CTTTCGCTCC TTGAACTTGC ATCTAAACTC GACCTCTACA 6181 TTTTTTATGT TTATCTCTAG TATTACTCTT TAGACAAAAA AATTGTAGTA AGAACTATTC 6241 ATAGAGTGAA TCGAAAACAA TACGAAAATG TAAACATTTC CTATACGTAG TATATAGAGA 6301 CAAAATAGAA GAAACCGTTC ATAATTTTCT GACCAATGAA GAATCATCAA CGCTATCACT 6361 TTCTGTTCAC AAAGTATGCG CAATCCACAT CGGTATAGAA TATAATCGGG GATGCCTTTA 6421 TCTTGAAAAA ATGCACCCGC AGCTTCGCTA GTAATCAGTA AACGCGGGAA GTGGAGTCAG 6481 GCTTTTTTTA TGGAAGAGAA AATAGACACC AAAGTAGCCT TCTTCTAACC TTAACGGACC 6541 TACAGTGCAA AAAGTTATCA AGAGACTGCA TTATAGAGCG CACAAAGGAG AAAAAAAGTA 6601 ATCTAAGATG CTTTGTTAGA AAAATAGCGC TCTCGGGATG CATTTTTGTA GAACAAAAAA 6661 GAAGTATAGA TTCTTTGTTG GTAAAATAGC GCTCTCGCGT TGCATTTCTG TTCTGTAAAA 6721 ATGCAGCTCA GATTCTTTGT TTGAAAAATT AGCGCTCTCG CGTTGCATTT TTGTTTTACA 6781 AAAATGAAGC ACAGATTCTT CGTTGGTAAA ATAGCGCTTT CGCGTTGCAT TTCTGTTCTG 6841 TAAAAATGCA GCTCAGATTC TTTGTTTGAA AAATTAGCGC TCTCGCGTTG CATTTTTGTT 6901 CTACAAAATG AAGCACAGAT GCTTCGTTGC TTGCATGCCT GCAGGTCGAG ATCCGGGATC 6961 GAAGAAATGA TGGTAAATGA AATAGGAAAT CAAGGAGCAT GAAGGCAAAA GACAAATATA 7021 AGGGTCGAAC GAAAAATAAA GTGAAAAGTG TTGATATGAT GTATTTGGCT TTGCGGCGCC 7081 GAAAAAACGA GTTTACGCAA TTGCACAATC ATGCTGACTC TGTGGCGGAC CCGCGCTCTT 7141 GCCGGCCCGG CGATAACGCT GGGCGTGAGG CTGTGCCCGG CGGAGTTTTT TGCGCCTGCA 7201 TTTTCCAAGG TTTACCCTGC GCTAAGGGGC GAGATTGGAG AAGCAATAAG AATGCCGGTT 7261 GGGGTTGCGA TGATGACGAC CACGACAACT GGTGTCATTA TTTAAGTTGC CGAAAGAACC 7321 TGAGTGCATT TGCAACATGA GTATACTAGA AGAATGAGCC AAGACTTGCG AGACGCGAGT 7381 TTGCCGGTGG TGCGAACAAT AGAGCGACCA TGACCTTGAA GGTGAGACGC GCATAACCGC 7441 TAGAGTACTT TGAAGAGGAA ACAGCAATAG GGTTGCTACC AGTATAAATA GACAGGTACA 7501 TACAACACTG GAAATGGTTG TCTGTTTGAG TACGCTTTCA ATTCATTTGG GTGTGCACTT 7561 TATTATGTTA CAATATGGAA GGGAACTTTA CACTTCTCCT ATGCACATAT ATTAATTAAA 7621 GTCCAATGCT AGTAGAGAAG GGGGGTAACA CCCCTCCGCG CTCTTTTCCG ATTTTTTTCT 7681 AAACCGTGGA ATATTTCGGA TATCCTTTTG TTGTTTCCGG GTGTACAATA TGGACTTCCT 7741 CTTTTCTGGC AACCAAACCC ATACATCGGG ATTCCTATAA TACCTTCGTT GGTCTCCCTA 7801 ACATGTAGGT GGCGGAGGGG AGATATACAA TAGAACAGAT ACCAGACAAG ACATAATGGG 7861 CTAAACAAGA CTACACCAAT TACACTGCCT CATTGATGGT GGTACATAAC GAACTAATAC 7921 TGTAGCCCTA GACTTGATAG CCATCATCAT ATCGAAGTTT CACTACCCTT TTTCCATTTG 7981 CCATCTATTG AAGTAATAAT AGGCGCATG // @ pGAD GH "Times New Roman"C@ yADH1_pro"Times New RomanCɆ@4Gal4_AD"Times New RomanC@SV40_NLS"Times New Roman@4Gal4_AD"Times New RomanC@ Gal4_AD_pri"Times New Roman*C@jzpBluescriptKS_pri"Times New RomanCɆ@T7_pro"Times New Roman@M13_forward20_pri"Times New RomanC@M13_pUC_fwd_pri"Times New RomanCɆ@  yADH2_term"Times New RomanC@pJG4_5_rev_pri"Times New RomanC@O yADH1_term"Times New Roman@pHybLex_rev_pri"Times New Roman*C@ ORF 1"Times New RomanCɆ@  LEU2(variant)"Times New Roman@ M13_reverse_pri"Times New RomanC@ M13_pUC_rev_pri"Times New RomanC@ lac_pro"Times New RomanCɆ@- N loxP"Times New Roman@ loxP"Times New RomanC@  pBR322_origin"Times New RomanC@ORF 2"Times New RomanCɆ@ Ampicillin"Times New Roman(@+GAmpR_pro"Times New RomanC@ pGEX_3_pri"Times New RomanC@2micron2_origin"Times New Roman GSpeI(829),BamHI(835),SmaI(843),EcoRI(853),SalI(880),XhoI(886),ApaI(899)"Times New Roman=@'-33{z MscI"Times New Roman[@MT[ [  AflII"Times New Roman @v NheI(3438),NotI(3460)"Times New Romann @zW AatII"Times New Roman @ \ NarI"Times New Roman@ test"Times New Romanx T@c-"?II test"Times New Roman #@4sUCHC