Sequence of pGAD

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Sequence of pGAD_GH

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Sequence of pGAD GH : 8009 BP; 2290 A; 1781 C; 1694 G; 2244 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 caacttcttt tctttttttt tcttttctct ctcccccgtt gttgtctcac catatccgca 60

1 gttgaagaaa agaaaaaaaa agaaaagaga gagggggcaa caacagagtg gtataggcgt 60

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 atgacaaaaa aaatgatgga agacactaaa ggaaaaaatt aacgacaaag acagcaccaa 120

61 tactgttttt tttactacct tctgtgattt ccttttttaa ttgctgtttc tgtcgtggtt 120

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cagatgtcgt tgttccagag ctgatgaggg gtatctcgaa cacacgaaac tttttccttc 180

121 gtctacagca acaaggtctc gactactccc catagagctt gtgtgctttg aaaaaggaag 180

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 cttcattcac gcacactact ctctaatgag caacggtata cggccttcct tccagttact 240

181 gaagtaagtg cgtgtgatga gagattactc gttgccatat gccggaagga aggtcaatga 240

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgaatttgaa ataaaaaaaa gtttgccgct ttgctatcaa gtataaatag acctgcaatt 300

241 acttaaactt tatttttttt caaacggcga aacgatagtt catatttatc tggacgttaa 300

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 attaatcttt tgtttcctcg tcattgttct cgttcccttt cttccttgtt tctttttctg 360

301 taattagaaa acaaaggagc agtaacaaga gcaagggaaa gaaggaacaa agaaaaagac 360

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 cacaatattt caagctatac caagcataca atcaactcca agctttgcaa agatggataa 420

361 gtgttataaa gttcgatatg gttcgtatgt tagttgaggt tcgaaacgtt tctacctatt 420

.......... yADH1_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 agcggaatta attcccgagc ctccaaaaaa gaagagaaag gtcgaattgg gtaccgccgc 480

421 tcgccttaat taagggctcg gaggtttttt cttctctttc cagcttaacc catggcggcg 480

.......... ....... Gal4_AD SV40_NLS ....... .......... ..........

.......... .......... .......... .......... .......... ..........

481 caattttaat caaagtggga atattgctga tagctcattg tccttcactt tcactaacag 540

481 gttaaaatta gtttcaccct tataacgact atcgagtaac aggaagtgaa agtgattgtc 540

.......... .......... Gal4_AD Gal4_AD .......... ..........

.......... .......... .......... .......... .......... ..........

541 tagcaacggt ccgaacctca taacaactca aacaaattct caagcgcttt cacaaccaat 600

541 atcgttgcca ggcttggagt attgttgagt ttgtttaaga gttcgcgaaa gtgttggtta 600

.......... ....... Gal4_AD Gal4_AD ....... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tgcctcctct aacgttcatg ataacttcat gaataatgaa atcacggcta gtaaaattga 660

601 acggaggaga ttgcaagtac tattgaagta cttattactt tagtgccgat cattttaact 660

.......... ....... Gal4_AD Gal4_AD ....... .......... ..........

.......... .......... .......... .......... .......... ..........

661 tgatggtaat aattcaaaac cactgtcacc tggttggacg gaccaaactg cgtataacgc 720

661 actaccatta ttaagttttg gtgacagtgg accaacctgc ctggtttgac gcatattgcg 720

.......... ....... Gal4_AD Gal4_AD ....... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gtttggaatc actacaggga tgtttaatac cactacaatg gatgatgtat ataactatct 780

721 caaaccttag tgatgtccct acaaattatg gtgatgttac ctactacata tattgataga 780

.......... ...... Gal4_AD Gal4_AD Gal4_AD_pri ...... ..........

.......... .......... .......... .......... SpeI(829) ..........

781 attcgatgat gaagataccc caccaaaccc aaaaaaagag atcctagaac tagtggatcc 840

781 taagctacta cttctatggg gtggtttggg tttttttctc taggatcttg atcacctagg 840

....... Gal4_AD Gal4_AD ....... .......... .......... ..........

SmaI843) EcoRI(853) .......... SalI(880) XhoI(886) ApaI(899)

841 cccgggctgc aggaattcga tatcaagctt atcgataccg tcgacctcga gggggggccc 900

841 gggcccgacg tccttaagct atagttcgaa tagctatggc agctggagct cccccccggg 900

.......... .......... .......... ...... pBluescriptKS_pri ......

.......... .......... .......... .......... .......... ..........

901 ggtacccaat tcgccctata gtgagtcgta ttacaattca ctggccgtcg ttttacaacg 960

901 ccatgggtta agcgggatat cactcagcat aatgttaagt gaccggcagc aaaatgttgc 960

.......... .......... T7_pro ... M13_forward20_pri M13_pUC_fwd_pri

.......... .......... .......... .......... .......... ..........

961 tcgtgactgg gaaaaccctg atctatgaat cgtagatact gaaaaacccc gcaagttcac 1020

961 agcactgacc cttttgggac tagatactta gcatctatga ctttttgggg cgttcaagtg 1020

M13_pUC_fwd_pri .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttcaactgtg catcgtgcac catctcaatt tctttcattt atacatcgtt ttgccttctt 1080

1021 aagttgacac gtagcacgtg gtagagttaa agaaagtaaa tatgtagcaa aacggaagaa 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... MscI(1115) .......... ..........

1081 ttatgtaact atactcctct aagtttcaat cttggccatg taacctctga tctatagaat 1140

1081 aatacattga tatgaggaga ttcaaagtta gaaccggtac attggagact agatatctta 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 tttttaaatg actagaatta atgcccatct tttttttgga cctaaattct tcatgaaaat 1200

1141 aaaaatttac tgatcttaat tacgggtaga aaaaaaacct ggatttaaga agtactttta 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 atattacgag ggcttattca gaagctttgg acttcttcgc cagaggtttg gtcaagtctc 1260

1201 tataatgctc ccgaataagt cttcgaaacc tgaagaagcg gtctccaaac cagttcagag 1260

.......... .......... .......... .. yADH2_term pJG4_5_rev_pri ..

.......... .......... .......... .......... .......... ..........

1261 caatcaaggt tgtcggcttg tctaccttgc cagaaattta cgaaaagatg gaaaagggtc 1320

1261 gttagttcca acagccgaac agatggaacg gtctttaaat gcttttctac cttttcccag 1320

.......... .......... yADH2_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 aaatcgttgg tagatacgtt gttgacactt ctaaataagc gaatttctta t gatttatga 1380

1321 tttagcaacc atctatgcaa caactgtgaa gatttattcg cttaaagaat actaaatact 1380

.......... .......... yADH2_term .......... yADH1_term ..........

.......... .......... .......... .......... .......... ..........

1381 tttttattat taaataagtt ataaaaaaaa taagtgtata caaattttaa agtgactctt 1440

1381 aaaaataata atttattcaa tatttttttt attcacatat gtttaaaatt tcactgagaa 1440

.......... .... yADH2_term yADH1_term . pHybLex_rev_pri .......

.......... .......... .......... .......... .......... ..........

1441 aggttttaaa acgaaaattc ttattcttga gtaactcttt cctgtaggtc aggttgcttt 1500

1441 tccaaaattt tgcttttaag aataagaact cattgagaaa ggacatccag tccaacgaaa 1500

.......... .... yADH2_term yADH1_term .... .......... ..........

.......... .......... .......... .......... .......... ..........

1501 ctcaggtata gcatgaggtc gctcttattg accacacctc taccggccgg tcgaaattcc 1560

1501 gagtccatat cgtactccag cgagaataac tggtgtggag atggccggcc agctttaagg 1560

.......... yADH1_term yADH2_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cctaccctat gaacatattc cattttgtaa tttcgtgtcg tttctattat gaatttcatt 1620

1561 ggatgggata cttgtataag gtaaaacatt aaagcacagc aaagataata cttaaagtaa 1620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tataaagttt atgtacaaat atcataaaaa aagagaatct ttttaagcaa ggattttctt 1680

1621 atatttcaaa tacatgttta tagtattttt ttctcttaga aaaattcgtt cctaaaagaa 1680

.......... .......... .......... .......... ..... ORF 1 LEU2(variant)

.......... .......... .......... .......... .......... ..........

1681 aacttcttcg gcgacagcat caccgacttc ggtggtactg ttggaaccac ctaaatcacc 1740

1681 ttgaagaagc cgctgtcgta gtggctgaag ccaccatgac aaccttggtg gatttagtgg 1740

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 agttctgata cctgcatcca aaaccttttt aactgcatct tcaatggcct taccttcttc 1800

1741 tcaagactat ggacgtaggt tttggaaaaa ttgacgtaga agttaccgga atggaagaag 1800

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aggcaagttc aatgacaatt tcaacatcat tgcagcagac aagatagtgg cgatagggtc 1860

1801 tccgttcaag ttactgttaa agttgtagta acgtcgtctg ttctatcacc gctatcccag 1860

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aaccttattc tttggcaaat ctggagcaga accgtggcat ggttcgtaca aaccaaatgc 1920

1861 ttggaataag aaaccgttta gacctcgtct tggcaccgta ccaagcatgt ttggtttacg 1920

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 ggtgttcttg tctggcaaag aggccaagga cgcagatggc aacaaaccca aggaacctgg 1980

1921 ccacaagaac agaccgtttc tccggttcct gcgtctaccg ttgtttgggt tccttggacc 1980

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gataacggag gcttcatcgg agatgatatc accaaacatg ttgctggtga ttataatacc 2040

1981 ctattgcctc cgaagtagcc tctactatag tggtttgtac aacgaccact aatattatgg 2040

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 atttaggtgg gttgggttct taactaggat catggcggca gaatcaatca attgatgttg 2100

2041 taaatccacc caacccaaga attgatccta gtaccgccgt cttagttagt taactacaac 2100

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 aaccttcaat gtaggaaatt cgttcttgat ggtttcctcc acagtttttc tccataatct 2160

2101 ttggaagtta catcctttaa gcaagaacta ccaaaggagg tgtcaaaaag aggtattaga 2160

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tgaagaggcc aaaacattag ctttatccaa ggaccaaata ggcaatggtg gctcatgttg 2220

2161 acttctccgg ttttgtaatc gaaataggtt cctggtttat ccgttaccac cgagtacaac 2220

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 tagggccatg aaagcggcca ttcttgtgat tctttgcact tctggaacgg tgtattgttc 2280

2221 atcccggtac tttcgccggt aagaacacta agaaacgtga agaccttgcc acataacaag 2280

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 actatcccaa gcgacaccat caccatcgtc ttcctttctc ttaccaaagt aaatacctcc 2340

2281 tgatagggtt cgctgtggta gtggtagcag aaggaaagag aatggtttca tttatggagg 2340

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cactaattct ctgacaacaa cgaagtcagt acctttagca aattgtggct tgattggaga 2400

2341 gtgattaaga gactgttgtt gcttcagtca tggaaatcgt ttaacaccga actaacctct 2400

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... ......... AflII(2438) ......... ..........

2401 taagtctaaa agagagtcgg atgcaaagtt acatggtctt aagttggcgt acaattgaag 2460

2401 attcagattt tctctcagcc tacgtttcaa tgtaccagaa ttcaaccgca tgttaacttc 2460

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 ttctttacgg atttttagta aaccttgttc aggtctaaca ctacctgtac cccatttagg 2520

2461 aagaaatgcc taaaaatcat ttggaacaag tccagattgt gatggacatg gggtaaatcc 2520

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 accacccaca gcacctaaca aaacggcatc aaccttcttg gaggcttcca gcgcctcatc 2580

2521 tggtgggtgt cgtggattgt tttgccgtag ttggaagaac ctccgaaggt cgcggagtag 2580

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 tggaagtggg acacctgtag catcgatagc agcaccacca attaaatgat tttcgaaatc 2640

2581 accttcaccc tgtggacatc gtagctatcg tcgtggtggt taatttacta aaagctttag 2640

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gaacttgaca ttggaacgaa catcagaaat agctttaaga accttaatgg cttcggctgt 2700

2641 cttgaactgt aaccttgctt gtagtcttta tcgaaattct tggaattacc gaagccgaca 2700

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gatttcttga ccaacgtggt cacctggcaa aacgacgatc ttcttagggg cagacattag 2760

2701 ctaaagaact ggttgcacca gtggaccgtt ttgctgctag aagaatcccc gtctgtaatc 2760

.......... ..... ORF 1 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 aatggtatat ccttgaaata tatatatata ttgctgaaat gtaaaaggta agaaaagtta 2820

2761 ttaccatata ggaactttat atatatatat aacgacttta cattttccat tcttttcaat 2820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gaaagtaaga cgattgctaa ccacctattg gaaaaaacaa taggtcctta aataatattg 2880

2821 ctttcattct gctaacgatt ggtggataac cttttttgtt atccaggaat ttattataac 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tcaacttcaa gtattgtgat gcaagcattt agtcatgaac gcttctctat tctatatgaa 2940

2881 agttgaagtt cataacacta cgttcgtaaa tcagtacttg cgaagagata agatatactt 2940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 aagccggttc cggcctctca cctttccttt ttctcccaat ttttcagttg aaaaaggtat 3000

2941 ttcggccaag gccggagagt ggaaaggaaa aagagggtta aaaagtcaac tttttccata 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 atgcgtcagg cgacctctga aattaacaaa aaatttccag tcatcgaatt tgattctgtg 3060

3001 tacgcagtcc gctggagact ttaattgttt tttaaaggtc agtagcttaa actaagacac 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 cgatagcgcc cctgtgtgtt ctcgttatgt tgaggaaaaa aataatggtt gctaagagat 3120

3061 gctatcgcgg ggacacacaa gagcaataca actccttttt ttattaccaa cgattctcta 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 tcgaactctt gcatcttacg atacctgagt attcccacag ttggggatct cgactctagc 3180

3121 agcttgagaa cgtagaatgc tatggactca taagggtgtc aacccctaga gctgagatcg 3180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tagaggatca attcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc 3240

3181 atctcctagt taagcattag taccagtatc gacaaaggac acactttaac aataggcgag 3240

.......... ....... M13_reverse_pri .... M13_pUC_rev_pri .......

.......... .......... .......... .......... .......... ..........

3241 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga 3300

3241 tgttaaggtg tgttgtatgc tcggccttcg tatttcacat ttcggacccc acggattact 3300

.......... .......... lac_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg 3360

3301 cactcgattg agtgtaatta acgcaacgcg agtgacgggc gaaaggtcag ccctttggac 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tcgtgccagc tgataacttc gtataatgta tgctatacga agttattagg tctgaagagg 3420

3361 agcacggtcg actattgaag catattacat acgatatgct tcaataatcc agacttctcc 3420

.......... .......... loxP .......... .......... ..........

.......... NheI(3438) .......... NotI(3460) .......... ..........

3421 agtttacgtc cagccaagct agcttggctg caggtcgagc ggccgcgatc cggaaccctt 3480

3421 tcaaatgcag gtcggttcga tcgaaccgac gtccagctcg ccggcgctag gccttgggaa 3480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 aatataactt cgtataatgt atgctatacg aagttatcag ctgcattaat gaatcggcca 3540

3481 ttatattgaa gcatattaca tacgatatgc ttcaatagtc gacgtaatta cttagccggt 3540

.......... loxP .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc 3600

3541 tgcgcgcccc tctccgccaa acgcataacc cgcgagaagg cgaaggagcg agtgactgag 3600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg 3660

3601 cgacgcgagc cagcaagccg acgccgctcg ccatagtcga gtgagtttcc gccattatgc 3660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa 3720

3661 caataggtgt cttagtcccc tattgcgtcc tttcttgtac actcgttttc cggtcgtttt 3720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga 3780

3721 ccggtccttg gcatttttcc ggcgcaacga ccgcaaaaag gtatccgagg cggggggact 3780

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

3781 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag 3840

3781 gctcgtagtg tttttagctg cgagttcagt ctccaccgct ttgggctgtc ctgatatttc 3840

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3841 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct 3900

3841 tatggtccgc aaagggggac cttcgaggga gcacgcgaga ggacaaggct gggacggcga 3900

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3901 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg 3960

3901 atggcctatg gacaggcgga aagagggaag cccttcgcac cgcgaaagag tatcgagtgc 3960

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

3961 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc 4020

3961 gacatccata gagtcaagcc acatccagca agcgaggttc gacccgacac acgtgcttgg 4020

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt 4080

4021 ggggcaagtc gggctggcga cgcggaatag gccattgata gcagaactca ggttgggcca 4080

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4081 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta 4140

4081 ttctgtgctg aatagcggtg accgtcgtcg gtgaccattg tcctaatcgt ctcgctccat 4140

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4141 tgtaggcggt gctaacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga 4200

4141 acatccgcca cgattgtctc aagaacttca ccaccggatt gatgccgatg tgatcttcct 4200

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4201 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct 4260

4201 gtcataaacc atagacgcga gacgacttcg gtcaatggaa gcctttttct caaccatcga 4260

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4261 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga 4320

4261 gaactaggcc gtttgtttgg tggcgaccat cgccaccaaa aaaacaaacg ttcgtcgtct 4320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4321 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg 4380

4321 aatgcgcgtc tttttttcct agagttcttc taggaaacta gaaaagatgc cccagactgc 4380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4381 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct 4440

4381 gagtcacctt gcttttgagt gcaattccct aaaaccagta ctctaatagt ttttcctaga 4440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt 4500

4441 agtggatcta ggaaaattta atttttactt caaaatttag ttagatttca tatatactca 4500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc 4560

4501 tttgaaccag actgtcaatg gttacgaatt agtcactccg tggatagagt cgctagacag 4560

.......... .......... ....... ORF 2 Ampicillin ....... ..........

.......... .......... .......... .......... .......... ..........

4561 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg 4620

4561 ataaagcaag taggtatcaa cggactgagg ggcagcacat ctattgatgc tatgccctcc 4620

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag 4680

4621 cgaatggtag accggggtca cgacgttact atggcgctct gggtgcgagt ggccgaggtc 4680

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt 4740

4681 taaatagtcg ttatttggtc ggtcggcctt cccggctcgc gtcttcacca ggacgttgaa 4740

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag 4800

4741 ataggcggag gtaggtcaga taattaacaa cggcccttcg atctcattca tcaagcggtc 4800

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt 4860

4801 aattatcaaa cgcgttgcaa caacggtaac gatgtccgta gcaccacagt gcgagcagca 4860

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca 4920

4861 aaccataccg aagtaagtcg aggccaaggg ttgctagttc cgctcaatgt actagggggt 4920

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg 4980

4921 acaacacgtt ttttcgccaa tcgaggaagc caggaggcta gcaacagtct tcattcaacc 4980

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat 5040

4981 ggcgtcacaa tagtgagtac caataccgtc gtgacgtatt aagagaatga cagtacggta 5040

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta 5100

5041 ggcattctac gaaaagacac tgaccactca tgagttggtt cagtaagact cttatcacat 5100

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca 5160

5101 acgccgctgg ctcaacgaga acgggccgca gttatgccct attatggcgc ggtgtatcgt 5160

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct 5220

5161 cttgaaattt tcacgagtag taaccttttg caagaagccc cgcttttgag agttcctaga 5220

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat 5280

5221 atggcgacaa ctctaggtca agctacattg ggtgagcacg tgggttgact agaagtcgta 5280

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa 5340

5281 gaaaatgaaa gtggtcgcaa agacccactc gtttttgtcc ttccgtttta cggcgttttt 5340

.......... ....... ORF 2 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt 5400

5341 tcccttattc ccgctgtgcc tttacaactt atgagtatga gaaggaaaaa gttataataa 5400

....... ORF 2 Ampicillin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa 5460

5401 cttcgtaaat agtcccaata acagagtact cgcctatgta taaacttaca taaatctttt 5460

.......... .......... .......... AmpR_pro .......... ..........

.......... .......... .......... .......... ......... AatII(5512)

5461 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa 5520

5461 tatttgttta tccccaaggc gcgtgtaaag gggcttttca cggtggactg cagattcttt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg 5580

5521 ggtaataata gtactgtaat tggatatttt tatccgcata gtgctccggg aaagcagagc 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag 5640

5581 gcgcaaagcc actactgcca cttttggaga ctgtgtacgt cgagggcctc tgccagtgtc 5640

.......... .......... .......... pGEX_3_pri .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg 5700

5641 gaacagacat tcgcctacgg ccctcgtctg ttcgggcagt cccgcgcagt cgcccacaac 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc 5760

5701 cgcccacagc cccgaccgaa ttgatacgcc gtagtctcgt ctaacatgac tctcacgtgg 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 ataacgcatt taagcataaa cacgcactat gccgttcttc tcatgtatat atatatacag 5820

5761 tattgcgtaa attcgtattt gtgcgtgata cggcaagaag agtacatata tatatatgtc 5820

.......... .......... ....... 2micron2_origin ....... ..........

.......... .......... .......... .......... .......... ..........

5821 gcaacacgca gatataggtg cgacgtgaac agtgagctgt atgtgcgcag ctcgcgttgc 5880

5821 cgttgtgcgt ctatatccac gctgcacttg tcactcgaca tacacgcgtc gagcgcaacg 5880

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 attttcggaa gcgctcgttt tcggaaacgc tttgaagttc ctattccgaa gttcctattc 5940

5881 taaaagcctt cgcgagcaaa agcctttgcg aaacttcaag gataaggctt caaggataag 5940

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 tctagctaga aagtatagga acttcagagc gcttttgaaa accaaaagcg ctctgaagac 6000

5941 agatcgatct ttcatatcct tgaagtctcg cgaaaacttt tggttttcgc gagacttctg 6000

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gcactttcaa aaaaccaaaa acgcaccgga ctgtaacgag ctactaaaat attgcgaata 6060

6001 cgtgaaagtt ttttggtttt tgcgtggcct gacattgctc gatgatttta taacgcttat 6060

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 ccgcttccac aaacattgct caaaagtatc tctttgctat atatctctgt gctatatccc 6120

6061 ggcgaaggtg tttgtaacga gttttcatag agaaacgata tatagagaca cgatataggg 6120

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tatataacct acccatccac ctttcgctcc ttgaacttgc atctaaactc gacctctaca 6180

6121 atatattgga tgggtaggtg gaaagcgagg aacttgaacg tagatttgag ctggagatgt 6180

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ttttttatgt ttatctctag tattactctt tagacaaaaa aattgtagta agaactattc 6240

6181 aaaaaataca aatagagatc ataatgagaa atctgttttt ttaacatcat tcttgataag 6240

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 atagagtgaa tcgaaaacaa tacgaaaatg taaacatttc ctatacgtag tatatagaga 6300

6241 tatctcactt agcttttgtt atgcttttac atttgtaaag gatatgcatc atatatctct 6300

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 caaaatagaa gaaaccgttc ataattttct gaccaatgaa gaatcatcaa cgctatcact 6360

6301 gttttatctt ctttggcaag tattaaaaga ctggttactt cttagtagtt gcgatagtga 6360

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ttctgttcac aaagtatgcg caatccacat cggtatagaa tataatcggg gatgccttta 6420

6361 aagacaagtg tttcatacgc gttaggtgta gccatatctt atattagccc ctacggaaat 6420

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 tcttgaaaaa atgcacccgc agcttcgcta gtaatcagta aacgcgggaa gtggagtcag 6480

6421 agaacttttt tacgtgggcg tcgaagcgat cattagtcat ttgcgccctt cacctcagtc 6480

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 gcttttttta tggaagagaa aatagacacc aaagtagcct tcttctaacc ttaacggacc 6540

6481 cgaaaaaaat accttctctt ttatctgtgg tttcatcgga agaagattgg aattgcctgg 6540

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 tacagtgcaa aaagttatca agagactgca ttatagagcg cacaaaggag aaaaaaagta 6600

6541 atgtcacgtt tttcaatagt tctctgacgt aatatctcgc gtgtttcctc tttttttcat 6600

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 atctaagatg ctttgttaga aaaatagcgc tctcgggatg catttttgta gaacaaaaaa 6660

6601 tagattctac gaaacaatct ttttatcgcg agagccctac gtaaaaacat cttgtttttt 6660

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gaagtataga ttctttgttg gtaaaatagc gctctcgcgt tgcatttctg ttctgtaaaa 6720

6661 cttcatatct aagaaacaac cattttatcg cgagagcgca acgtaaagac aagacatttt 6720

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 atgcagctca gattctttgt ttgaaaaatt agcgctctcg cgttgcattt ttgttttaca 6780

6721 tacgtcgagt ctaagaaaca aactttttaa tcgcgagagc gcaacgtaaa aacaaaatgt 6780

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 aaaatgaagc acagattctt cgttggtaaa atagcgcttt cgcgttgcat ttctgttctg 6840

6781 ttttacttcg tgtctaagaa gcaaccattt tatcgcgaaa gcgcaacgta aagacaagac 6840

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 taaaaatgca gctcagattc tttgtttgaa aaattagcgc tctcgcgttg catttttgtt 6900

6841 atttttacgt cgagtctaag aaacaaactt tttaatcgcg agagcgcaac gtaaaaacaa 6900

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 ctacaaaatg aagcacagat gcttcgttgc ttgcatgcct gcaggtcgag atccgggatc 6960

6901 gatgttttac ttcgtgtcta cgaagcaacg aacgtacgga cgtccagctc taggccctag 6960

....... 2micron2_origin ....... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gaagaaatga tggtaaatga aataggaaat caaggagcat gaaggcaaaa gacaaatata 7020

6961 cttctttact accatttact ttatccttta gttcctcgta cttccgtttt ctgtttatat 7020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... NarI(7076)

7021 agggtcgaac gaaaaataaa gtgaaaagtg ttgatatgat gtatttggct ttgcggcgcc 7080

7021 tcccagcttg ctttttattt cacttttcac aactatacta cataaaccga aacgccgcgg 7080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 gaaaaaacga gtttacgcaa ttgcacaatc atgctgactc tgtggcggac ccgcgctctt 7140

7081 cttttttgct caaatgcgtt aacgtgttag tacgactgag acaccgcctg ggcgcgagaa 7140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 gccggcccgg cgataacgct gggcgtgagg ctgtgcccgg cggagttttt tgcgcctgca 7200

7141 cggccgggcc gctattgcga cccgcactcc gacacgggcc gcctcaaaaa acgcggacgt 7200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 ttttccaagg tttaccctgc gctaaggggc gagattggag aagcaataag aatgccggtt 7260

7201 aaaaggttcc aaatgggacg cgattccccg ctctaacctc ttcgttattc ttacggccaa 7260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 ggggttgcga tgatgacgac cacgacaact ggtgtcatta tttaagttgc cgaaagaacc 7320

7261 ccccaacgct actactgctg gtgctgttga ccacagtaat aaattcaacg gctttcttgg 7320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 tgagtgcatt tgcaacatga gtatactaga agaatgagcc aagacttgcg agacgcgagt 7380

7321 actcacgtaa acgttgtact catatgatct tcttactcgg ttctgaacgc tctgcgctca 7380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 ttgccggtgg tgcgaacaat agagcgacca tgaccttgaa ggtgagacgc gcataaccgc 7440

7381 aacggccacc acgcttgtta tctcgctggt actggaactt ccactctgcg cgtattggcg 7440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 tagagtactt tgaagaggaa acagcaatag ggttgctacc agtataaata gacaggtaca 7500

7441 atctcatgaa acttctcctt tgtcgttatc ccaacgatgg tcatatttat ctgtccatgt 7500

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 tacaacactg gaaatggttg tctgtttgag tacgctttca attcatttgg gtgtgcactt 7560

7501 atgttgtgac ctttaccaac agacaaactc atgcgaaagt taagtaaacc cacacgtgaa 7560

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 tattatgtta caatatggaa gggaacttta cacttctcct atgcacatat attaattaaa 7620

7561 ataatacaat gttatacctt cccttgaaat gtgaagagga tacgtgtata taattaattt 7620

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 gtccaatgct agtagagaag gggggtaaca cccctccgcg ctcttttccg atttttttct 7680

7621 caggttacga tcatctcttc cccccattgt ggggaggcgc gagaaaaggc taaaaaaaga 7680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 aaaccgtgga atatttcgga tatccttttg ttgtttccgg gtgtacaata tggacttcct 7740

7681 tttggcacct tataaagcct ataggaaaac aacaaaggcc cacatgttat acctgaagga 7740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 cttttctggc aaccaaaccc atacatcggg attcctataa taccttcgtt ggtctcccta 7800

7741 gaaaagaccg ttggtttggg tatgtagccc taaggatatt atggaagcaa ccagagggat 7800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 acatgtaggt ggcggagggg agatatacaa tagaacagat accagacaag acataatggg 7860

7801 tgtacatcca ccgcctcccc tctatatgtt atcttgtcta tggtctgttc tgtattaccc 7860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 ctaaacaaga ctacaccaat tacactgcct cattgatggt ggtacataac gaactaatac 7920

7861 gatttgttct gatgtggtta atgtgacgga gtaactacca ccatgtattg cttgattatg 7920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 tgtagcccta gacttgatag ccatcatcat atcgaagttt cactaccctt tttccatttg 7980

7921 acatcgggat ctgaactatc ggtagtagta tagcttcaaa gtgatgggaa aaaggtaaac 7980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 ccatctattg aagtaataat aggcgcatg. .......... .......... .......... 8009

7981 ggtagataac ttcattatta tccgcgtac. .......... .......... .......... 8009

.......... .......... .......... .......... .......... ..........