Sequence of pGAD

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Sequence of pGAD_T7

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Sequence of pGAD T7 : 7988 BP; 2284 A; 1774 C; 1696 G; 2234 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 tgcatgcctg caggtcgaga tccgggatcg aagaaatgat ggtaaatgaa ataggaaatc 60

1 acgtacggac gtccagctct aggccctagc ttctttacta ccatttactt tatcctttag 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 aaggagcatg aaggcaaaag acaaatataa gggtcgaacg aaaaataaag tgaaaagtgt 120

61 ttcctcgtac ttccgttttc tgtttatatt cccagcttgc tttttatttc acttttcaca 120

.......... .......... .......... .......... .......... ..........

.......... .......... NarI(145) .......... .......... ..........

121 tgatatgatg tatttggctt tgcggcgccg aaaaaacgag tttacgcaat tgcacaatca 180

121 actatactac ataaaccgaa acgccgcggc ttttttgctc aaatgcgtta acgtgttagt 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 tgctgactct gtggcggacc cgcgctcttg ccggcccggc gataacgctg ggcgtgaggc 240

181 acgactgaga caccgcctgg gcgcgagaac ggccgggccg ctattgcgac ccgcactccg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 tgtgcccggc ggagtttttt gcgcctgcat tttccaaggt ttaccctgcg ctaaggggcg 300

241 acacgggccg cctcaaaaaa cgcggacgta aaaggttcca aatgggacgc gattccccgc 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 agattggaga agcaataaga atgccggttg gggttgcgat gatgacgacc acgacaactg 360

301 tctaacctct tcgttattct tacggccaac cccaacgcta ctactgctgg tgctgttgac 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gtgtcattat ttaagttgcc gaaagaacct gagtgcattt gcaacatgag tatactagaa 420

361 cacagtaata aattcaacgg ctttcttgga ctcacgtaaa cgttgtactc atatgatctt 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gaatgagcca agacttgcga gacgcgagtt tgccggtggt gcgaacaata gagcgaccat 480

421 cttactcggt tctgaacgct ctgcgctcaa acggccacca cgcttgttat ctcgctggta 480

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 gaccttgaag gtgagacgcg cataaccgct agagtacttt gaagaggaaa cagcaatagg 540

481 ctggaacttc cactctgcgc gtattggcga tctcatgaaa cttctccttt gtcgttatcc 540

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 gttgctacca gtataaatag acaggtacat acaacactgg aaatggttgt ctgtttgagt 600

541 caacgatggt catatttatc tgtccatgta tgttgtgacc tttaccaaca gacaaactca 600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 acgctttcaa ttcatttggg tgtgcacttt attatgttac aatatggaag ggaactttac 660

601 tgcgaaagtt aagtaaaccc acacgtgaaa taatacaatg ttataccttc ccttgaaatg 660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 acttctccta tgcacatata ttaattaaag tccaatgcta gtagagaagg ggggtaacac 720

661 tgaagaggat acgtgtatat aattaatttc aggttacgat catctcttcc ccccattgtg 720

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ccctccgcgc tcttttccga tttttttcta aaccgtggaa tatttcggat atccttttgt 780

721 gggaggcgcg agaaaaggct aaaaaaagat ttggcacctt ataaagccta taggaaaaca 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 tgtttccggg tgtacaatat ggacttcctc ttttctggca accaaaccca tacatcggga 840

781 acaaaggccc acatgttata cctgaaggag aaaagaccgt tggtttgggt atgtagccct 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 ttcctataat accttcgttg gtctccctaa catgtaggtg gcggagggga gatatacaat 900

841 aaggatatta tggaagcaac cagagggatt gtacatccac cgcctcccct ctatatgtta 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 agaacagata ccagacaaga cataatgggc taaacaagac tacaccaatt acactgcctc 960

901 tcttgtctat ggtctgttct gtattacccg atttgttctg atgtggttaa tgtgacggag 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 attgatggtg gtacataacg aactaatact gtagccctag acttgatagc catcatcata 1020

961 taactaccac catgtattgc ttgattatga catcgggatc tgaactatcg gtagtagtat 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tcgaagtttc actacccttt ttccatttgc catctattga agtaataata ggcgcatgca 1080

1021 agcttcaaag tgatgggaaa aaggtaaacg gtagataact tcattattat ccgcgtacgt 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 acttcttttc tttttttttc ttttctctct cccccgttgt tgtctcacca tatccgcaat 1140

1081 tgaagaaaag aaaaaaaaag aaaagagaga gggggcaaca acagagtggt ataggcgtta 1140

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 gacaaaaaaa atgatggaag acactaaagg aaaaaattaa cgacaaagac agcaccaaca 1200

1141 ctgttttttt tactaccttc tgtgatttcc ttttttaatt gctgtttctg tcgtggttgt 1200

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gatgtcgttg ttccagagct gatgaggggt atctcgaagc acacgaaact ttttccttcc 1260

1201 ctacagcaac aaggtctcga ctactcccca tagagcttcg tgtgctttga aaaaggaagg 1260

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 ttcattcacg cacactactc tctaatgagc aacggtatac ggccttcctt ccagttactt 1320

1261 aagtaagtgc gtgtgatgag agattactcg ttgccatatg ccggaaggaa ggtcaatgaa 1320

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 gaatttgaaa taaaaaaaag tttgctgtct tgctatcaag tataaataga cctgcaatta 1380

1321 cttaaacttt attttttttc aaacgacaga acgatagttc atatttatct ggacgttaat 1380

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt ctttttctgc 1440

1381 aattagaaaa caaaggagca gtaacaagag caagggaaag aaggaacaaa gaaaaagacg 1440

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 acaatatttc aagctatacc aagcatacaa tcaactccaa gctttgcaaa gatggataaa 1500

1441 tgttataaag ttcgatatgg ttcgtatgtt agttgaggtt cgaaacgttt ctacctattt 1500

.......... yADH1_pro .......... .......... .......... Gal4_AD

.......... .......... .......... .......... .......... KpnI(1553)

1501 gcggaattaa ttcccgagcc tccaaaaaag aagagaaagg tcgaattggg taccgccgcc 1560

1501 cgccttaatt aagggctcgg aggttttttc ttctctttcc agcttaaccc atggcggcgg 1560

.......... ....... Gal4_AD SV40_NLS ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 aattttaatc aaagtgggaa tattgctgat agctcattgt ccttcacttt cactaacagt 1620

1561 ttaaaattag tttcaccctt ataacgacta tcgagtaaca ggaagtgaaa gtgattgtca 1620

.......... ....... Gal4_AD Gal4_AD ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 agcaacggtc cgaacctcat aacaactcaa acaaattctc aagcgctttc acaaccaatt 1680

1621 tcgttgccag gcttggagta ttgttgagtt tgtttaagag ttcgcgaaag tgttggttaa 1680

.......... ....... Gal4_AD Gal4_AD ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gcctcctcta acgttcatga taacttcatg aataatgaaa tcacggctag taaaattgat 1740

1681 cggaggagat tgcaagtact attgaagtac ttattacttt agtgccgatc attttaacta 1740

.......... ....... Gal4_AD Gal4_AD ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gatggtaata attcaaaacc actgtcacct ggttggacgg accaaactgc gtataacgcg 1800

1741 ctaccattat taagttttgg tgacagtgga ccaacctgcc tggtttgacg catattgcgc 1800

.......... ....... Gal4_AD Gal4_AD ....... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tttggaatca ctacagggat gtttaatacc actacaatgg atgatgtata taactatcta 1860

1801 aaaccttagt gatgtcccta caaattatgg tgatgttacc tactacatat attgatagat 1860

.......... ...... Gal4_AD Gal4_AD Gal4_AD_pri ...... ..........

.......... .......... .......... .......... .......... ..........

1861 ttcgatgatg aagatacccc accaaaccca aaaaaagaga tctttaatac gactcactat 1920

1861 aagctactac ttctatgggg tggtttgggt ttttttctct agaaattatg ctgagtgata 1920

....... Gal4_AD Gal4_AD ....... .......... .......... T7_pro

.......... .......... .......... .......... .......... ..........

1921 agggcgagcg ccgccatgga gtacccatac gacgtaccag attacgctca tatggccatg 1980

1921 tcccgctcgc ggcggtacct catgggtatg ctgcatggtc taatgcgagt ataccggtac 1980

.......... .......... .......... .......... .......... ..........

EcoRI SmaI ClaI BamHI(2018) XhoI(2030),SacI(2031)

1981 gaggccagtg aattccaccc gggtgggcat cgatacggga tccatcgagc tcgagctgca 2040

1981 ctccggtcac ttaaggtggg cccacccgta gctatgccct aggtagctcg agctcgacgt 2040

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 gatgaatcgt agatactgaa aaaccccgca agttcacttc aactgtgcat cgtgcaccat 2100

2041 ctacttagca tctatgactt tttggggcgt tcaagtgaag ttgacacgta gcacgtggta 2100

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctcaatttct ttcatttata catcgttttg ccttctttta tgtaactata ctcctctaag 2160

2101 gagttaaaga aagtaaatat gtagcaaaac ggaagaaaat acattgatat gaggagattc 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 tttcaatctt ggccatgtaa cctctgatct atagaatttt ttaaatgact agaattaatg 2220

2161 aaagttagaa ccggtacatt ggagactaga tatcttaaaa aatttactga tcttaattac 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 cccatctttt ttttggacct aaattcttca tgaaaatata ttacgagggc ttattcagaa 2280

2221 gggtagaaaa aaaacctgga tttaagaagt acttttatat aatgctcccg aataagtctt 2280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 gctttggact tcttcgccag aggtttggtc aagtctccaa tcaaggttgt cggcttgtct 2340

2281 cgaaacctga agaagcggtc tccaaaccag ttcagaggtt agttccaaca gccgaacaga 2340

.......... .. yADH2_term pJG4_5_rev_pri .. .......... ..........

.......... .......... .......... .......... .......... ..........

2341 accttgccag aaatttacga aaagatggaa aagggtcaaa tcgttggtag atacgttgtt 2400

2341 tggaacggtc tttaaatgct tttctacctt ttcccagttt agcaaccatc tatgcaacaa 2400

.......... .......... yADH2_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gacacttcta aataagcgaa tttcttatga tttatgattt ttattattaa ataagttata 2460

2401 ctgtgaagat ttattcgctt aaagaatact aaatactaaa aataataatt tattcaatat 2460

.......... .......... yADH2_term yADH1_term .......... ..........

.......... .......... .......... .......... .......... ..........

2461 aaaaaaataa gtgtatacaa attttaaagt gactcttagg ttttaaaacg aaaattctta 2520

2461 tttttttatt cacatatgtt taaaatttca ctgagaatcc aaaattttgc ttttaagaat 2520

...... yADH2_term yADH1_term pHybLex_rev_pri ...... ..........

.......... .......... .......... .......... .......... ..........

2521 ttcttgagta actctttcct gtaggtcagg ttgctttctc aggtatagca tgaggtcgct 2580

2521 aagaactcat tgagaaagga catccagtcc aacgaaagag tccatatcgt actccagcga 2580

.......... .... yADH2_term yADH1_term .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cttattgacc acacctctac cggccggtcg aaattcccct accctatgaa catattccat 2640

2581 gaataactgg tgtggagatg gccggccagc tttaagggga tgggatactt gtataaggta 2640

yADH1_term yADH2_term .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tttgtaattt cgtgtcgttt ctattatgaa tttcatttat aaagtttatg tacaaatatc 2700

2641 aaacattaaa gcacagcaaa gataatactt aaagtaaata tttcaaatac atgtttatag 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 ataaaaaaag agaatctttt taagcaagga ttttcttaac ttcttcggcg acagcatcac 2760

2701 tatttttttc tcttagaaaa attcgttcct aaaagaattg aagaagccgc tgtcgtagtg 2760

.......... .......... ..... ORF 2 LEU2(variant) ..... ..........

.......... .......... .......... .......... .......... ..........

2761 cgacttcggt ggtactgttg gaaccaccta aatcaccagt tctgatacct gcatccaaaa 2820

2761 gctgaagcca ccatgacaac cttggtggat ttagtggtca agactatgga cgtaggtttt 2820

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cctttttaac tgcatcttca atggccttac cttcttcagg caagttcaat gacaatttca 2880

2821 ggaaaaattg acgtagaagt taccggaatg gaagaagtcc gttcaagtta ctgttaaagt 2880

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 acatcattgc agcagacaag atagtggcga tagggttgac cttattcttt ggcaaatctg 2940

2881 tgtagtaacg tcgtctgttc tatcaccgct atcccaactg gaataagaaa ccgtttagac 2940

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 gagcagaacc gtggcatggt tcgtacaaac caaatgcggt gttcttgtct ggcaaagagg 3000

2941 ctcgtcttgg caccgtacca agcatgtttg gtttacgcca caagaacaga ccgtttctcc 3000

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 ccaaggacgc agatggcaac aaacccaagg aacctgggat aacggaggct tcatcggaga 3060

3001 ggttcctgcg tctaccgttg tttgggttcc ttggacccta ttgcctccga agtagcctct 3060

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 tgatatcacc aaacatgttg ctggtgatta taataccatt taggtgggtt gggttcttaa 3120

3061 actatagtgg tttgtacaac gaccactaat attatggtaa atccacccaa cccaagaatt 3120

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ctaggatcat ggcggcagaa tcaatcaatt gatgttgaac cttcaatgta ggaaattcgt 3180

3121 gatcctagta ccgccgtctt agttagttaa ctacaacttg gaagttacat cctttaagca 3180

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3181 tcttgatggt ttcctccaca gtttttctcc ataatcttga agaggccaaa acattagctt 3240

3181 agaactacca aaggaggtgt caaaaagagg tattagaact tctccggttt tgtaatcgaa 3240

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tatccaagga ccaaataggc aatggtggct catgttgtag ggccatgaaa gcggccattc 3300

3241 ataggttcct ggtttatccg ttaccaccga gtacaacatc ccggtacttt cgccggtaag 3300

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 ttgtgattct ttgcacttct ggaacggtgt attgttcact atcccaagcg acaccatcac 3360

3301 aacactaaga aacgtgaaga ccttgccaca taacaagtga tagggttcgc tgtggtagtg 3360

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 catcgtcttc ctttctctta ccaaagtaaa tacctcccac taattctctg acaacaacga 3420

3361 gtagcagaag gaaagagaat ggtttcattt atggagggtg attaagagac tgttgttgct 3420

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agtcagtacc tttagcaaat tgtggcttga ttggagataa gtctaaaaga gagtcggatg 3480

3421 tcagtcatgg aaatcgttta acaccgaact aacctctatt cagattttct ctcagcctac 3480

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

......... AflII(3495) ......... .......... .......... ..........

3481 caaagttaca tggtcttaag ttggcgtaca attgaagttc tttacggatt tttagtaaac 3540

3481 gtttcaatgt accagaattc aaccgcatgt taacttcaag aaatgcctaa aaatcatttg 3540

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 cttgttcagg tctaacacta cctgtacccc atttaggacc acccacagca cctaacaaaa 3600

3541 gaacaagtcc agattgtgat ggacatgggg taaatcctgg tgggtgtcgt ggattgtttt 3600

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 cggcatcaac cttcttggag gcttccagcg cctcatctgg aagtgggaca cctgtagcgt 3660

3601 gccgtagttg gaagaacctc cgaaggtcgc ggagtagacc ttcaccctgt ggacatcgca 3660

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 cgatagcagc accaccaatt aaatgatttt cgaaatcgaa cttgacattg gaacgaacat 3720

3661 gctatcgtcg tggtggttaa tttactaaaa gctttagctt gaactgtaac cttgcttgta 3720

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cagaaatagc tttaagaacc ttaatggctt cggctgtgat ttcttgacca acgtggtcac 3780

3721 gtctttatcg aaattcttgg aattaccgaa gccgacacta aagaactggt tgcaccagtg 3780

.......... ..... ORF 2 LEU2(variant) ..... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 ctggcaaaac gacgatcttc ttaggggcag acattagaat ggtatatcct tgaaatatat 3840

3781 gaccgttttg ctgctagaag aatccccgtc tgtaatctta ccatatagga actttatata 3840

..... ORF 2 LEU2(variant) ..... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 atatatattg ctgaaatgta aaaggtaaga aaagttagaa agtaagacga ttgctaacca 3900

3841 tatatataac gactttacat tttccattct tttcaatctt tcattctgct aacgattggt 3900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 cctattggaa aaaacaatag gtccttaaat aatattgtca acttcaagta ttgtgatgca 3960

3901 ggataacctt ttttgttatc caggaattta ttataacagt tgaagttcat aacactacgt 3960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agcatttagt catgaacgct tctctattct atatgaaaag ccggttccgg cgctctcacc 4020

3961 tcgtaaatca gtacttgcga agagataaga tatacttttc ggccaaggcc gcgagagtgg 4020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 tttccttttt ctcccaattt ttcagttgaa aaaggtatat gcgtcaggcg acctctgaaa 4080

4021 aaaggaaaaa gagggttaaa aagtcaactt tttccatata cgcagtccgc tggagacttt 4080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4081 ttaacaaaaa atttccagtc atcgaatttg attctgtgcg atagcgcccc tgtgtgttct 4140

4081 aattgttttt taaaggtcag tagcttaaac taagacacgc tatcgcgggg acacacaaga 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 cgttatgttg aggaaaaaaa taatggttgc taagagattc gaactcttgc atcttacgat 4200

4141 gcaatacaac tccttttttt attaccaacg attctctaag cttgagaacg tagaatgcta 4200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4201 acctgagtat tcccacagtt ggggatctcg actctagcta gaggatcaat tcgtaatcat 4260

4201 tggactcata agggtgtcaa cccctagagc tgagatcgat ctcctagtta agcattagta 4260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 4320

4261 ccagtatcga caaaggacac actttaacaa taggcgagtg ttaaggtgtg ttgtatgctc 4320

M13_reverse_pri . M13_pUC_rev_pri ....... .......... lac_pro

.......... .......... .......... .......... .......... ..........

4321 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 4380

4321 ggccttcgta tttcacattt cggaccccac ggattactca ctcgattgag tgtaattaac 4380

lac_pro .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg ataacttcgt 4440

4381 gcaacgcgag tgacgggcga aaggtcagcc ctttggacag cacggtcgac tattgaagca 4440

.......... .......... .......... .......... .......... loxP

.......... .......... .......... .......... .......... ..........

4441 ataatgtatg ctatacgaag ttattaggtc tgaagaggag tttacgtcca gccaagctag 4500

4441 tattacatac gatatgcttc aataatccag acttctcctc aaatgcaggt cggttcgatc 4500

.......... loxP .......... .......... .......... ..........

.......... NotI(4518) .......... .......... .......... ..........

4501 cttggctgca ggtcgagcgg ccgcgatccg gaacccttaa tataacttcg tataatgtat 4560

4501 gaaccgacgt ccagctcgcc ggcgctaggc cttgggaatt atattgaagc atattacata 4560

.......... .......... .......... .......... .......... loxP

.......... .......... .......... .......... .......... ..........

4561 gctatacgaa gttatcagct gcattaatga atcggccaac gcgcggggag aggcggtttg 4620

4561 cgatatgctt caatagtcga cgtaattact tagccggttg cgcgcccctc tccgccaaac 4620

loxP .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg 4680

4621 gcataacccg cgagaaggcg aaggagcgag tgactgagcg acgcgagcca gcaagccgac 4680

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat 4740

4681 gccgctcgcc atagtcgagt gagtttccgc cattatgcca ataggtgtct tagtccccta 4740

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc 4800

4741 ttgcgtcctt tcttgtacac tcgttttccg gtcgttttcc ggtccttggc atttttccgg 4800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc 4860

4801 cgcaacgacc gcaaaaaggt atccgaggcg gggggactgc tcgtagtgtt tttagctgcg 4860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4861 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga 4920

4861 agttcagtct ccaccgcttt gggctgtcct gatatttcta tggtccgcaa agggggacct 4920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4921 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt 4980

4921 tcgagggagc acgcgagagg acaaggctgg gacggcgaat ggcctatgga caggcggaaa 4980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

4981 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg 5040

4981 gagggaagcc cttcgcaccg cgaaagagta tcgagtgcga catccataga gtcaagccac 5040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5041 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc 5100

5041 atccagcaag cgaggttcga cccgacacac gtgcttgggg ggcaagtcgg gctggcgacg 5100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5101 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg 5160

5101 cggaataggc cattgatagc agaactcagg ttgggccatt ctgtgctgaa tagcggtgac 5160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5161 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc 5220

5161 cgtcgtcggt gaccattgtc ctaatcgtct cgctccatac atccgccacg atgtctcaag 5220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5221 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg 5280

5221 aacttcacca ccggattgat gccgatgtga tcttcctgtc ataaaccata gacgcgagac 5280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5281 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc 5340

5281 gacttcggtc aatggaagcc tttttctcaa ccatcgagaa ctaggccgtt tgtttggtgg 5340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct 5400

5341 cgaccatcgc caccaaaaaa acaaacgttc gtcgtctaat gcgcgtcttt ttttcctaga 5400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

5401 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt 5460

5401 gttcttctag gaaactagaa aagatgcccc agactgcgag tcaccttgct tttgagtgca 5460

pBR322_origin ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa 5520

5461 attccctaaa accagtactc taatagtttt tcctagaagt ggatctagga aaatttaatt 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa 5580

5521 tttacttcaa aatttagtta gatttcatat atactcattt gaaccagact gtcaatggtt 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc 5640

5581 acgaattagt cactccgtgg atagagtcgc tagacagata aagcaagtag gtatcaacgg 5640

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct 5700

5641 actgaggggc agcacatcta ttgatgctat gccctcccga atggtagacc ggggtcacga 5700

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca 5760

5701 cgttactatg gcgctctggg tgcgagtggc cgaggtctaa atagtcgtta tttggtcggt 5760

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt 5820

5761 cggccttccc ggctcgcgtc ttcaccagga cgttgaaata ggcggaggta ggtcagataa 5820

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt 5880

5821 ttaacaacgg cccttcgatc tcattcatca agcggtcaat tatcaaacgc gttgcaacaa 5880

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc 5940

5881 cggtaacgat gtccgtagca ccacagtgcg agcagcaaac cataccgaag taagtcgagg 5940

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5941 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc 6000

5941 ccaagggttg ctagttccgc tcaatgtact agggggtaca acacgttttt tcgccaatcg 6000

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt 6060

6001 aggaagccag gaggctagca acagtcttca ttcaaccggc gtcacaatag tgagtaccaa 6060

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6061 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact 6120

6061 taccgtcgtg acgtattaag agaatgacag tacggtaggc attctacgaa aagacactga 6120

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc 6180

6121 ccactcatga gttggttcag taagactctt atcacatacg ccgctggctc aacgagaacg 6180

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt 6240

6181 ggccgcagtt atgccctatt atggcgcggt gtatcgtctt gaaattttca cgagtagtaa 6240

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg 6300

6241 ccttttgcaa gaagccccgc ttttgagagt tcctagaatg gcgacaactc taggtcaagc 6300

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6301 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct 6360

6301 tacattgggt gagcacgtgg gttgactaga agtcgtagaa aatgaaagtg gtcgcaaaga 6360

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa 6420

6361 cccactcgtt tttgtccttc cgttttacgg cgttttttcc cttattcccg ctgtgccttt 6420

.......... ....... ORF 3 Ampicillin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt 6480

6421 acaacttatg agtatgagaa ggaaaaagtt ataataactt cgtaaatagt cccaataaca 6480

ORF 3 Ampicillin .... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc 6540

6481 gagtactcgc ctatgtataa acttacataa atctttttat ttgtttatcc ccaaggcgcg 6540

.......... AmpR_pro .......... .......... .......... ..........

.......... ......... AatII(6569) ......... .......... ..........

6541 acatttcccc gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc 6600

6541 tgtaaagggg cttttcacgg tggactgcag attctttggt aataatagta ctgtaattgg 6600

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 tataaaaata ggcgtatcac gaggcccttt cgtctcgcgc gtttcggtga tgacggtgaa 6660

6601 atatttttat ccgcatagtg ctccgggaaa gcagagcgcg caaagccact actgccactt 6660

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg 6720

6661 ttggagactg tgtacgtcga gggcctctgc cagtgtcgaa cagacattcg cctacggccc 6720

.......... pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg ctggcttaac 6780

6721 tcgtctgttc gggcagtccc gcgcagtcgc ccacaaccgc ccacagcccc gaccgaattg 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 tatgcggcat cagagcagat tgtactgaga gtgcaccata acgcatttaa gcataaacac 6840

6781 atacgccgta gtctcgtcta acatgactct cacgtggtat tgcgtaaatt cgtatttgtg 6840

.......... .......... .......... ....... 2micron2_origin .......

.......... .......... .......... .......... .......... ..........

6841 gcactatgcc gttcttctca tgtatatata tatacaggca acacgcagat ataggtgcga 6900

6841 cgtgatacgg caagaagagt acatatatat atatgtccgt tgtgcgtcta tatccacgct 6900

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 cgtgaacagt gagctgtatg tgcgcagctc gcgttgcatt ttcggaagcg ctcgttttcg 6960

6901 gcacttgtca ctcgacatac acgcgtcgag cgcaacgtaa aagccttcgc gagcaaaagc 6960

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

6961 gaaacgcttt gaagttccta ttccgaagtt cctattctct agctagaaag tataggaact 7020

6961 ctttgcgaaa cttcaaggat aaggcttcaa ggataagaga tcgatctttc atatccttga 7020

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 tcagagcgct tttgaaaacc aaaagcgctc tgaagacgca ctttcaaaaa accaaaaacg 7080

7021 agtctcgcga aaacttttgg ttttcgcgag acttctgcgt gaaagttttt tggtttttgc 7080

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 caccggactg taacgagcta ctaaaatatt gcgaataccg cttccacaaa cattgctcaa 7140

7081 gtggcctgac attgctcgat gattttataa cgcttatggc gaaggtgttt gtaacgagtt 7140

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 aagtatctct ttgctatata tctctgtgct atatccctat ataacctacc catccacctt 7200

7141 ttcatagaga aacgatatat agagacacga tatagggata tattggatgg gtaggtggaa 7200

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 tcgctccttg aacttgcatc taaactcgac ctctacattt tttatgttta tctctagtat 7260

7201 agcgaggaac ttgaacgtag atttgagctg gagatgtaaa aaatacaaat agagatcata 7260

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 tactctttag acaaaaaaat tgtagtaaga actattcata gagtgaatcg aaaacaatac 7320

7261 atgagaaatc tgttttttta acatcattct tgataagtat ctcacttagc ttttgttatg 7320

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7321 gaaaatgtaa acatttccta tacgtagtat atagagacaa aatagaagaa accgttcata 7380

7321 cttttacatt tgtaaaggat atgcatcata tatctctgtt ttatcttctt tggcaagtat 7380

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7381 attttctgac caatgaagaa tcatcaacgc tatcactttc tgttcacaaa gtatgcgcaa 7440

7381 taaaagactg gttacttctt agtagttgcg atagtgaaag acaagtgttt catacgcgtt 7440

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7441 tccacatcgg tatagaatat aatcggggat gcctttatct tgaaaaaatg cacccgcagc 7500

7441 aggtgtagcc atatcttata ttagccccta cggaaataga acttttttac gtgggcgtcg 7500

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7501 ttcgctagta atcagtaaac gcgggaagtg gagtcaggct ttttttatgg aagagaaaat 7560

7501 aagcgatcat tagtcatttg cgcccttcac ctcagtccga aaaaaatacc ttctctttta 7560

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7561 agacaccaaa gtagccttct tctaacctta acggacctac agtgcaaaaa gttatcaaga 7620

7561 tctgtggttt catcggaaga agattggaat tgcctggatg tcacgttttt caatagttct 7620

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7621 gactgcatta tagagcgcac aaaggagaaa aaaagtaatc taagatgctt tgttagaaaa 7680

7621 ctgacgtaat atctcgcgtg tttcctcttt ttttcattag attctacgaa acaatctttt 7680

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7681 atagcgctct cgggatgcat ttttgtagaa caaaaaagaa gtatagattc tttgttggta 7740

7681 tatcgcgaga gccctacgta aaaacatctt gttttttctt catatctaag aaacaaccat 7740

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7741 aaatagcgct ctcgcgttgc atttctgttc tgtaaaaatg cagctcagat tctttgtttg 7800

7741 tttatcgcga gagcgcaacg taaagacaag acatttttac gtcgagtcta agaaacaaac 7800

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7801 aaaaattagc gctctcgcgt tgcatttttg ttttacaaaa atgaagcaca gattcttcgt 7860

7801 tttttaatcg cgagagcgca acgtaaaaac aaaatgtttt tacttcgtgt ctaagaagca 7860

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7861 tggtaaaata gcgctttcgc gttgcatttc tgttctgtaa aaatgcagct cagattcttt 7920

7861 accattttat cgcgaaagcg caacgtaaag acaagacatt tttacgtcga gtctaagaaa 7920

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7921 gtttgaaaaa ttagcgctct cgcgttgcat ttttgttcta caaaatgaag cacagatgct 7980

7921 caaacttttt aatcgcgaga gcgcaacgta aaaacaagat gttttacttc gtgtctacga 7980

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

7981 tcgttgct.. .......... .......... .......... .......... .......... 7988

7981 agcaacga.. .......... .......... .......... .......... .......... 7988

.......... .......... .......... .......... .......... ..........