Sequence of pGAPZ

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Sequence of pGAPZ_A

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Sequence of pGAPZ A : 2882 BP; 673 A; 732 C; 675 G; 802 T; 0 other;

BglII(1) .......... .......... .......... .......... ..........

1 agatcttttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca tctctgaaat 60

1 tctagaaaaa acatctttac agaaccacag gagcaggtta gtccatcggt agagacttta 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc cggggtaaaa 120

61 tagaccgagg caacgttgag gcttgctgga cgaccgttgc attttaagag gccccatttt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct tccaccgccc 180

121 gaatttacac ctcattacct tggtctttgc agagaaggga agagagagga aggtggcggg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc cccttgcagc 240

181 caatggcagg gatcctttaa aatgagacga cctctcgaag aagatgccgg gggaacgtcg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg acctagcagc 300

241 ttacgagaag ggtcgtaatg caacgcccat tttgcctcca gcacatgggc tggatcgtcg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg catgtcatga 360

301 ggtccctacc ttttcagggc cggcagcgac cgttattatc gcccgcctgc gtacagtact 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca attttggttt 420

361 ctaataacct ttggtggtct tagcttatat tttccgcttg tggaaagggt taaaaccaaa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca attgaacaac 480

421 gaggactggg tttctgaaat ttaaattaaa taaacaggga taaagttagt taacttgttg 480

.......... .......... .......... .......... pGAP_pri ..........

BstBI(485) EcoRI(495) PmlI(502) .......... .......... ..........

481 tatttcgaaa cgaggaattc acgtggccca gccggccgtc tcggatcggt acctcgagcc 540

481 ataaagcttt gctccttaag tgcaccgggt cggccggcag agcctagcca tggagctcgg 540

.......... .......... .......... .......... .......... ..........

SacII(542),NotI(545) ApaI(562) .......... .......... ..........

541 gcggcggccg ccagctttct agaacaaaaa ctcatctcag aagaggatct gaatagcgcc 600

541 cgccgccggc ggtcgaaaga tcttgttttt gagtagagtc ttctcctaga cttatcgcgg 600

.......... .......... .......... c_myc .......... ..........

.......... .......... .......... .......... .......... ..........

601 gtcgaccatc atcatcatca tcattgagtt ttagccttag acatgactgt tcctcagttc 660

601 cagctggtag tagtagtagt agtaactcaa aatcggaatc tgtactgaca aggagtcaag 660

.......... 6xHis .......... .......... .......... ..........

.......... .......... AgeI(684) .......... .......... ..........

661 aagttgggca cttacgagaa gaccggtctt gctagattct aatcaagagg atgtcagaat 720

661 ttcaacccgt gaatgctctt ctggccagaa cgatctaaga ttagttctcc tacagtctta 720

.......... .......... .......... .......... .......... 3_AOX1_pri

.......... .......... .......... .......... .......... ..........

721 gccatttgcc tgagagatgc aggcttcatt tttgatactt ttttatttgt aacctatata 780

721 cggtaaacgg actctctacg tccgaagtaa aaactatgaa aaaataaaca ttggatatat 780

3_AOX1_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gtataggatt ttttttgtca ttttgtttct tctcgtacga gcttgctcct gatcagccta 840

781 catatcctaa aaaaaacagt aaaacaaaga agagcatgct cgaacgagga ctagtcggat 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 tctcgcagct gatgaatatc ttgtggtagg ggtttgggaa aatcattcga gtttgatgtt 900

841 agagcgtcga ctacttatag aacaccatcc ccaaaccctt ttagtaagct caaactacaa 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 tttcttggta tttcccactc ctcttcagag tacagaagat taagtgagac cttcgtttgt 960

901 aaagaaccat aaagggtgag gagaagtctc atgtcttcta attcactctg gaagcaaaca 960

.......... .......... .......... .......... .......... ..........

BamHI(965) .......... .......... .......... .......... ..........

961 gcggatcccc cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat 1020

961 cgcctagggg gtgtgtggta tcgaagtttt acaaagatga ggaaaaaatg agaaggtcta 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 tttctcggac tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat 1080

1021 aaagagcctg aggcgcgtag cggcatggtg aagttttgtg ggttcgtgtc gtatgattta 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tttccctctt tcttcctcta gggtgtcgtt aattacccgt actaaaggtt tggaaaagaa 1140

1081 aaagggagaa agaaggagat cccacagcaa ttaatgggca tgatttccaa accttttctt 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aaaagagacc gcctcgtttc tttttcttcg tcgaaaaagg caataaaaat ttttatcacg 1200

1141 ttttctctgg cggagcaaag aaaaagaagc agctttttcc gttattttta aaaatagtgc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 tttctttttc ttgaaatttt tttttttagt ttttttctct ttcagtgacc tccattgata 1260

1201 aaagaaaaag aactttaaaa aaaaaaatca aaaaaagaga aagtcactgg aggtaactat 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 tttaagttaa taaacggtct tcaatttctc aagtttcagt ttcatttttc ttgttctatt 1320

1261 aaattcaatt atttgccaga agttaaagag ttcaaagtca aagtaaaaag aacaagataa 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 acaacttttt ttacttcttg ttcattagaa agaaagcata gcaatctaat ctaagggcgg 1380

1321 tgttgaaaaa aatgaagaac aagtaatctt tctttcgtat cgttagatta gattcccgcc 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 tgttgacaat taatcatcgg catagtatat cggcatagta taatacgaca aggtgaggaa 1440

1381 acaactgtta attagtagcc gtatcatata gccgtatcat attatgctgt tccactcctt 1440

.......... .......... .......... EM7_pro .......... ..........

.... NcoI(1448),MscI(1453) .... .......... .......... ..........

1441 ctaaaccatg gccaagttga ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg 1500

1441 gatttggtac cggttcaact ggtcacggca aggccacgag tggcgcgcgc tgcagcggcc 1500

EM7_pro .......... ......... bleo sh_ble ......... ..........

.......... .......... .......... SmaI(1540) .......... ..........

1501 agcggtcgag ttctggaccg accggctcgg gttctcccgg gacttcgtgg aggacgactt 1560

1501 tcgccagctc aagacctggc tggccgagcc caagagggcc ctgaagcacc tcctgctgaa 1560

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 cgccggtgtg gtccgggacg acgtgaccct gttcatcagc gcggtccagg accaggtggt 1620

1561 gcggccacac caggccctgc tgcactggga caagtagtcg cgccaggtcc tggtccacca 1620

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gccggacaac accctggcct gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg 1680

1621 cggcctgttg tgggaccgga cccacaccca cgcgccggac ctgctcgaca tgcggctcac 1680

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 gtcggaggtc gtgtccacga acttccggga cgcctccggg ccggccatga ccgagatcgg 1740

1681 cagcctccag cacaggtgct tgaaggccct gcggaggccc ggccggtact ggctctagcc 1740

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 cgagcagccg tgggggcggg agttcgccct gcgcgacccg gccggcaact gcgtgcactt 1800

1741 gctcgtcggc acccccgccc tcaagcggga cgcgctgggc cggccgttga cgcacgtgaa 1800

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 cgtggccgag gagcaggact gacacgtccg acggcggccc acgggtccca ggcctcggag 1860

1801 gcaccggctc ctcgtcctga ctgtgcaggc tgccgccggg tgcccagggt ccggagcctc 1860

......... bleo sh_ble ......... .......... CYC1_term ..........

.......... ......... EcoRV(1888) ......... .......... ..........

1861 atccgtcccc cttttccttt gtcgatatca tgtaattagt tatgtcacgc ttacattcac 1920

1861 taggcagggg gaaaaggaaa cagctatagt acattaatca atacagtgcg aatgtaagtg 1920

.......... .......... CYC1_term .......... ... CYC1_pri pYESTrp_rev_pri

.......... .......... .......... .......... .......... ..........

1921 gccctccccc cacatccgct ctaaccgaaa aggaaggagt tagacaacct gaagtctagg 1980

1921 cgggaggggg gtgtaggcga gattggcttt tccttcctca atctgttgga cttcagatcc 1980

.......... .......... CYC1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 tccctattta tttttttata gttatgttag tattaagaac gttatttata tttcaaattt 2040

1981 agggataaat aaaaaaatat caatacaatc ataattcttg caataaatat aaagtttaaa 2040

.......... .......... CYC1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 ttcttttttt tctgtacaga cgcgtgtacg catgtaacat tatactgaaa accttgcttg 2100

2041 aagaaaaaaa agacatgtct gcgcacatgc gtacattgta atatgacttt tggaacgaac 2100

.......... .......... CYC1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 agaaggtttt gggacgctcg aaggctttaa tttgcaagct ggagaccaac atgtgagcaa 2160

2101 tcttccaaaa ccctgcgagc ttccgaaatt aaacgttcga cctctggttg tacactcgtt 2160

.......... .......... CYC1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc 2220

2161 ttccggtcgt tttccggtcc ttggcatttt tccggcgcaa cgaccgcaaa aaggtatccg 2220

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2221 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga 2280

2221 aggcgggggg actgctcgta gtgtttttag ctgcgagttc agtctccacc gctttgggct 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc 2340

2281 gtcctgatat ttctatggtc cgcaaagggg gaccttcgag ggagcacgcg agaggacaag 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt 2400

2341 gctgggacgg cgaatggcct atggacaggc ggaaagaggg aagcccttcg caccgcgaaa 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 ctcaatgctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct 2460

2401 gagttacgag tgcgacatcc atagagtcaa gccacatcca gcaagcgagg ttcgacccga 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg 2520

2461 cacacgtgct tggggggcaa gtcgggctgg cgacgcggaa taggccattg atagcagaac 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta 2580

2521 tcaggttggg ccattctgtg ctgaatagcg gtgaccgtcg tcggtgacca ttgtcctaat 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct 2640

2581 cgtctcgctc catacatccg ccacgatgtc tcaagaactt caccaccgga ttgatgccga 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa 2700

2641 tgtgatcttc ctgtcataaa ccatagacgc gagacgactt cggtcaatgg aagccttttt 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt 2760

2701 ctcaaccatc gagaactagg ccgtttgttt ggtggcgacc atcgccacca aaaaaacaaa 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta 2820

2761 cgttcgtcgt ctaatgcgcg tctttttttc ctagagttct tctaggaaac tagaaaagat 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgcatgaga 2880

2821 gccccagact gcgagtcacc ttgcttttga gtgcaattcc ctaaaaccag tacgtactct 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 tc........ .......... .......... .......... .......... .......... 2882

2881 ag........ .......... .......... .......... .......... .......... 2882

.......... .......... .......... .......... .......... ..........