Sequence of pGAPZ

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Sequence of pGAPZ_C

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Sequence of pGAPZ C : 2883 BP; 674 A; 732 C; 676 G; 801 T; 0 other;

BglII(1) .......... .......... .......... .......... ..........

1 agatcttttt tgtagaaatg tcttggtgtc ctcgtccaat caggtagcca tctctgaaat 60

1 tctagaaaaa acatctttac agaaccacag gagcaggtta gtccatcggt agagacttta 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 atctggctcc gttgcaactc cgaacgacct gctggcaacg taaaattctc cggggtaaaa 120

61 tagaccgagg caacgttgag gcttgctgga cgaccgttgc attttaagag gccccatttt 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 cttaaatgtg gagtaatgga accagaaacg tctcttccct tctctctcct tccaccgccc 180

121 gaatttacac ctcattacct tggtctttgc agagaaggga agagagagga aggtggcggg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gttaccgtcc ctaggaaatt ttactctgct ggagagcttc ttctacggcc cccttgcagc 240

181 caatggcagg gatcctttaa aatgagacga cctctcgaag aagatgccgg gggaacgtcg 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 aatgctcttc ccagcattac gttgcgggta aaacggaggt cgtgtacccg acctagcagc 300

241 ttacgagaag ggtcgtaatg caacgcccat tttgcctcca gcacatgggc tggatcgtcg 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ccagggatgg aaaagtcccg gccgtcgctg gcaataatag cgggcggacg catgtcatga 360

301 ggtccctacc ttttcagggc cggcagcgac cgttattatc gcccgcctgc gtacagtact 360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gattattgga aaccaccaga atcgaatata aaaggcgaac acctttccca attttggttt 420

361 ctaataacct ttggtggtct tagcttatat tttccgcttg tggaaagggt taaaaccaaa 420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 ctcctgaccc aaagacttta aatttaattt atttgtccct atttcaatca attgaacaac 480

421 gaggactggg tttctgaaat ttaaattaaa taaacaggga taaagttagt taacttgttg 480

.......... .......... .......... .......... pGAP_pri ..........

BstBI(485) EcoRI(495) PmlI(502) .......... .......... ..........

481 tatttcgaaa cgaggaattc acgtggccca gccggccgtc tcggatcggt acctcgagcc 540

481 ataaagcttt gctccttaag tgcaccgggt cggccggcag agcctagcca tggagctcgg 540

.......... .......... .......... .......... .......... ..........

SacII(542),NotI(545) .......... .......... .......... ..........

541 gcggcggccg ccagcttacg tagaacaaaa actcatctca gaagaggatc tgaatagcgc 600

541 cgccgccggc ggtcgaatgc atcttgtttt tgagtagagt cttctcctag acttatcgcg 600

.......... .......... .......... c_myc .......... ..........

.......... .......... .......... .......... .......... ..........

601 cgtcgaccat catcatcatc atcattgagt tttagcctta gacatgactg ttcctcagtt 660

601 gcagctggta gtagtagtag tagtaactca aaatcggaat ctgtactgac aaggagtcaa 660

.......... 6xHis .......... .......... .......... ..........

.......... .......... AgeI(683) .......... .......... ..........

661 caagttgggc acttacgaga agaccggtct tgctagattc taatcaagag gatgtcagaa 720

661 gttcaacccg tgaatgctct tctggccaga acgatctaag attagttctc ctacagtctt 720

.......... .......... .......... .......... .......... 3_AOX1_pri

.......... .......... .......... .......... .......... ..........

721 tgccatttgc ctgagagatg caggcttcat ttttgatact tttttatttg taacctatat 780

721 acggtaaacg gactctctac gtccgaagta aaaactatga aaaaataaac attggatata 780

3_AOX1_pri .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 agtataggat tttttttgtc attttgtttc ttctcgtacg agcttgctcc tgatcagcct 840

781 tcatatccta aaaaaaacag taaaacaaag aagagcatgc tcgaacgagg actagtcgga 840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 atctcgcagc tgatgaatat cttgtggtag gggtttggga aaatcattcg agtttgatgt 900

841 tagagcgtcg actacttata gaacaccatc cccaaaccct tttagtaagc tcaaactaca 900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ttttcttggt atttcccact cctcttcaga gtacagaaga ttaagtgaga ccttcgtttg 960

901 aaaagaacca taaagggtga ggagaagtct catgtcttct aattcactct ggaagcaaac 960

.......... .......... .......... .......... .......... ..........

BamHI(964) .......... .......... .......... .......... ..........

961 tgcggatccc ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga 1020

961 acgcctaggg ggtgtgtggt atcgaagttt tacaaagatg aggaaaaaat gagaaggtct 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 ttttctcgga ctccgcgcat cgccgtacca cttcaaaaca cccaagcaca gcatactaaa 1080

1021 aaaagagcct gaggcgcgta gcggcatggt gaagttttgt gggttcgtgt cgtatgattt 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 ttttccctct ttcttcctct agggtgtcgt taattacccg tactaaaggt ttggaaaaga 1140

1081 aaaagggaga aagaaggaga tcccacagca attaatgggc atgatttcca aaccttttct 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 aaaaagagac cgcctcgttt ctttttcttc gtcgaaaaag gcaataaaaa tttttatcac 1200

1141 tttttctctg gcggagcaaa gaaaaagaag cagctttttc cgttattttt aaaaatagtg 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gtttcttttt cttgaaattt ttttttttag tttttttctc tttcagtgac ctccattgat 1260

1201 caaagaaaaa gaactttaaa aaaaaaaatc aaaaaaagag aaagtcactg gaggtaacta 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 atttaagtta ataaacggtc ttcaatttct caagtttcag tttcattttt cttgttctat 1320

1261 taaattcaat tatttgccag aagttaaaga gttcaaagtc aaagtaaaaa gaacaagata 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tacaactttt tttacttctt gttcattaga aagaaagcat agcaatctaa tctaagggcg 1380

1321 atgttgaaaa aaatgaagaa caagtaatct ttctttcgta tcgttagatt agattcccgc 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga 1440

1381 cacaactgtt aattagtagc cgtatcatat agccgtatca tattatgctg ttccactcct 1440

.......... .......... EM7_pro .......... .......... ..........

.... NcoI(1447),MscI(1452) .... .......... .......... ..........

1441 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg 1500

1441 tgatttggta ccggttcaac tggtcacggc aaggccacga gtggcgcgcg ctgcagcggc 1500

.......... .......... ......... bleo sh_ble ......... ..........

.......... .......... .......... SmaI(1539) .......... ..........

1501 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact 1560

1501 ctcgccagct caagacctgg ctggccgagc ccaagagggc cctgaagcac ctcctgctga 1560

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg 1620

1561 agcggccaca ccaggccctg ctgcactggg acaagtagtc gcgccaggtc ctggtccacc 1620

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt 1680

1621 acggcctgtt gtgggaccgg acccacaccc acgcgccgga cctgctcgac atgcggctca 1680

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg 1740

1681 ccagcctcca gcacaggtgc ttgaaggccc tgcggaggcc cggccggtac tggctctagc 1740

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact 1800

1741 cgctcgtcgg cacccccgcc ctcaagcggg acgcgctggg ccggccgttg acgcacgtga 1800

.......... ......... bleo sh_ble ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 tcgtggccga ggagcaggac tgacacgtcc gacggcggcc cacgggtccc a ggcctcgga 1860

1801 agcaccggct cctcgtcctg actgtgcagg ctgccgccgg gtgcccaggg tccggagcct 1860

bleo sh_ble .......... .......... CYC1_term ..........

.......... ......... EcoRV(1887) ......... .......... ..........

1861 gatccgtccc ccttttcctt tgtcgatatc atgtaattag ttatgtcacg cttacattca 1920

1861 ctaggcaggg ggaaaaggaa acagctatag tacattaatc aatacagtgc gaatgtaagt 1920

.......... .......... CYC1_term .......... ... CYC1_pri pYESTrp_rev_pri

.......... .......... .......... .......... .......... ..........

1921 cgccctcccc ccacatccgc tctaaccgaa aaggaaggag ttagacaacc tgaagtctag 1980

1921 gcgggagggg ggtgtaggcg agattggctt ttccttcctc aatctgttgg acttcagatc 1980

.......... .......... CYC1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 gtccctattt atttttttat agttatgtta gtattaagaa cgttatttat atttcaaatt 2040

1981 cagggataaa taaaaaaata tcaatacaat cataattctt gcaataaata taaagtttaa 2040

.......... .......... CYC1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tttctttttt ttctgtacag acgcgtgtac gcatgtaaca ttatactgaa aaccttgctt 2100

2041 aaagaaaaaa aagacatgtc tgcgcacatg cgtacattgt aatatgactt ttggaacgaa 2100

.......... .......... CYC1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 gagaaggttt tgggacgctc gaaggcttta atttgcaagc tggagaccaa catgtgagca 2160

2101 ctcttccaaa accctgcgag cttccgaaat taaacgttcg acctctggtt gtacactcgt 2160

.......... .......... CYC1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg 2220

2161 tttccggtcg ttttccggtc cttggcattt ttccggcgca acgaccgcaa aaaggtatcc 2220

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2221 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg 2280

2221 gaggcggggg gactgctcgt agtgttttta gctgcgagtt cagtctccac cgctttgggc 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt 2340

2281 tgtcctgata tttctatggt ccgcaaaggg ggaccttcga gggagcacgc gagaggacaa 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt 2400

2341 ggctgggacg gcgaatggcc tatggacagg cggaaagagg gaagcccttc gcaccgcgaa 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 tctcaatgct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc 2460

2401 agagttacga gtgcgacatc catagagtca agccacatcc agcaagcgag gttcgacccg 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt 2520

2461 acacacgtgc ttggggggca agtcgggctg gcgacgcgga ataggccatt gatagcagaa 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt 2580

2521 ctcaggttgg gccattctgt gctgaatagc ggtgaccgtc gtcggtgacc attgtcctaa 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc 2640

2581 tcgtctcgct ccatacatcc gccacgatgt ctcaagaact tcaccaccgg attgatgccg 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tacactagaa ggacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa 2700

2641 atgtgatctt cctgtcataa accatagacg cgagacgact tcggtcaatg gaagcctttt 2700

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2701 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt 2760

2701 tctcaaccat cgagaactag gccgtttgtt tggtggcgac catcgccacc aaaaaaacaa 2760

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct 2820

2761 acgttcgtcg tctaatgcgc gtcttttttt cctagagttc ttctaggaaa ctagaaaaga 2820

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2821 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgcatgag 2880

2821 tgccccagac tgcgagtcac cttgcttttg agtgcaattc cctaaaacca gtacgtactc 2880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 atc....... .......... .......... .......... .......... .......... 2883

2881 tag....... .......... .......... .......... .......... .......... 2883

.......... .......... .......... .......... .......... ..........