Sequence of pGBK

This sequnce graph is created using BVTech Plasmid, which is DNA sequence analysis and plasmid drawing software for Windows PCs.You can use it to plan your DNA cloning, draw high quality plasmid maps, analyse your DNA sequencing data, align sequences, and much more.

Free to try, $39.98 to buy

Payments will be processed by PayPal. PayPal is the safer, easier way to pay.

Sequence of pGBK_T7

Download the sequence and plasmid map, and then use BVTech Plasmid to view and edit the file.

If you do not have BVTech Plasmid, download the latest version of BVTech Plasmid here.

View the plasmid map on line

Sequence of pGBK T7 : 7304 BP; 1976 A; 1650 C; 1739 G; 1939 T; 0 other;

.......... .......... .......... .......... .......... ..........

1 cggtgcgggc ctcttcgcta ttacgccaga tccttttgtt gtttccgggt gtacaatatg 60

1 gccacgcccg gagaagcgat aatgcggtct aggaaaacaa caaaggccca catgttatac 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 gacttcctct tttctggcaa ccaaacccat acatcgggat tcctataata ccttcgttgg 120

61 ctgaaggaga aaagaccgtt ggtttgggta tgtagcccta aggatattat ggaagcaacc 120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

121 tctccctaac atgtaggtgg cggaggggag atatacaata gaacagatac cagacaagac 180

121 agagggattg tacatccacc gcctcccctc tatatgttat cttgtctatg gtctgttctg 180

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ataatgggct aaacaagact acaccaatta cactgcctca ttgatggtgg tacataacga 240

181 tattacccga tttgttctga tgtggttaat gtgacggagt aactaccacc atgtattgct 240

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 actaatactg tagccctaga cttgatagcc atcatcatat cgaagtttca ctaccctttt 300

241 tgattatgac atcgggatct gaactatcgg tagtagtata gcttcaaagt gatgggaaaa 300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 tccatttgcc atctattgaa gtaataatag gcgcatgcaa cttcttttct ttttttttct 360

301 aggtaaacgg tagataactt cattattatc cgcgtacgtt gaagaaaaga aaaaaaaaga 360

.......... .......... .......... .......... yADH1_pro ..........

.......... .......... .......... .......... .......... ..........

361 tttctctctc ccccgttgtt gtctcaccat atccgcaatg acaaaaaaaa tgatggaaga 420

361 aaagagagag ggggcaacaa cagagtggta taggcgttac tgtttttttt actaccttct 420

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 cactaaagga aaaaattaac gacaaagaca gcaccaacag atgtcgttgt tccagagctg 480

421 gtgatttcct tttttaattg ctgtttctgt cgtggttgtc tacagcaaca aggtctcgac 480

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 atgaggggta tctcgaagca cacgaaactt tttccttcct tcattcacgc acactactct 540

481 tactccccat agagcttcgt gtgctttgaa aaaggaagga agtaagtgcg tgtgatgaga 540

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 ctaatgagca acggtatacg gccttccttc cagttacttg aatttgaaat aaaaaaagtt 600

541 gattactcgt tgccatatgc cggaaggaag gtcaatgaac ttaaacttta tttttttcaa 600

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 tgctgtcttg ctatcaagta taaatagacc tgcaattatt aatcttttgt ttcctcgtca 660

601 acgacagaac gatagttcat atttatctgg acgttaataa ttagaaaaca aaggagcagt 660

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ttgttctcgt tccctttctt ccttgtttct ttttctgcac aatatttcaa gctataccaa 720

661 aacaagagca agggaaagaa ggaacaaaga aaaagacgtg ttataaagtt cgatatggtt 720

.......... .......... yADH1_pro .......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 gcatacaatc aactccaagc ttgaagcaag cctcctgaaa gatgaagcta ctgtcttcta 780

721 cgtatgttag ttgaggttcg aacttcgttc ggaggacttt ctacttcgat gacagaagat 780

yADH1_pro .......... .......... .......... .......... Gal4_DBD

.......... .......... .......... .......... .......... ..........

781 tcgaacaagc atgcgatatt tgccgactta aaaagctcaa gtgctccaaa gaaaaaccga 840

781 agcttgttcg tacgctataa acggctgaat ttttcgagtt cacgaggttt ctttttggct 840

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 agtgcgccaa gtgtctgaag aacaactggg agtgtcgcta ctctcccaaa accaaaaggt 900

841 tcacgcggtt cacagacttc ttgttgaccc tcacagcgat gagagggttt tggttttcca 900

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 ctccgctgac tagggcacat ctgacagaag tggaatcaag gctagaaaga ctggaacagc 960

901 gaggcgactg atcccgtgta gactgtcttc accttagttc cgatctttct gaccttgtcg 960

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tatttctact gatttttcct cgagaagacc ttgacatgat tttgaaaatg gattctttac 1020

961 ataaagatga ctaaaaagga gctcttctgg aactgtacta aaacttttac ctaagaaatg 1020

.......... .......... Gal4_DBD .......... .......... ..........

.......... HpaI(1039) .......... .......... .......... ..........

1021 aggatataaa agcattgtta acaggattat ttgtacaaga taatgtgaat aaagatgccg 1080

1021 tcctatattt tcgtaacaat tgtcctaata aacatgttct attacactta tttctacggc 1080

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 tcacagatag attggcttca gtggagactg atatgcctct aacattgaga cagcatagaa 1140

1081 agtgtctatc taaccgaagt cacctctgac tatacggaga ttgtaactct gtcgtatctt 1140

.......... .......... Gal4_DBD .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 taagtgcgac atcatcatcg gaagagagta gtaacaaagg tcaaagacag ttgactgtat 1200

1141 attcacgctg tagtagtagc cttctctcat cattgtttcc agtttctgtc aactgacata 1200

.......... ....... Gal4_DBD Gal4_N_term_pri ....... ..........

.......... .......... .......... .......... .......... ..........

1201 cgccggaatt tgtaatacga ctcactatag ggcgagccgc catcatggag gagcagaagc 1260

1201 gcggccttaa acattatgct gagtgatatc ccgctcggcg gtagtacctc ctcgtcttcg 1260

.......... .......... T7_pro .......... .......... ..........

....... NdeI NcoI1288) EcoRI(1299) SmaI(1306),BamHI(1309)

1261 tgatctcaga ggaggacctg catatggcca tggaggccga attcccgggg atccgtcgac 1320

1261 actagagtct cctcctggac gtataccggt acctccggct taagggcccc taggcagctg 1320

.......... .......... .......... .......... .......... ..........

PstI(1325),NotI(1327) ........ .......... .......... ..........

1321 ctgcagcggc cgcataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt 1380

1321 gacgtcgccg gcgtattgat cgtattgggg aaccccggag atttgcccag aactccccaa 1380

.......... .......... .......... T7_term .......... ..........

.......... .......... .......... .......... .......... ..........

1381 ttttgcgcgc ttgcagccaa gctaattccg ggcgaatttc ttatgattta tgatttttat 1440

1381 aaaacgcgcg aacgtcggtt cgattaaggc ccgcttaaag aatactaaat actaaaaata 1440

.......... .......... .......... .......... yADH1_term ..........

.......... .......... .......... .......... .......... ..........

1441 tattaaataa gttataaaaa aaataagtgt atacaaattt taaagtgact cttaggtttt 1500

1441 ataatttatt caatattttt tttattcaca tatgtttaaa atttcactga gaatccaaaa 1500

.......... ....... yADH1_term pHybLex_rev_pri ....... ..........

.......... .......... .......... .......... .......... ..........

1501 aaaacgaaaa ttcttattct tgagtaactc tttcctgtag gtcaggttgc tttctcaggt 1560

1501 ttttgctttt aagaataaga actcattgag aaaggacatc cagtccaacg aaagagtcca 1560

.......... .......... yADH1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1561 atagcatgag gtcgctctta ttgaccacac ctctaccggc atgcaagctt ggcgtaatca 1620

1561 tatcgtactc cagcgagaat aactggtgtg gagatggccg tacgttcgaa ccgcattagt 1620

.......... .......... yADH1_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tggtcatagc tgtttcctgt gtgaaattgt tatccgctca caattccaca caacatacga 1680

1621 accagtatcg acaaaggaca cactttaaca ataggcgagt gttaaggtgt gttgtatgct 1680

M13_reverse_pri . M13_pUC_rev_pri ....... .......... lac_pro

.......... .......... .......... .......... .......... ..........

1681 gccggaagca taaagtgtaa agcctggggt gcctaatgag tgaggtaact cacattaatt 1740

1681 cggccttcgt atttcacatt tcggacccca cggattactc actccattga gtgtaattaa 1740

.......... lac_pro .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1741 gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt cgtgccagct gcattaatga 1800

1741 cgcaacgcga gtgacgggcg aaaggtcagc cctttggaca gcacggtcga cgtaattact 1800

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1801 atcggccaac gcgcggggag aggcggtttg cgtattgggc gctcttccgc ttcctcgctc 1860

1801 tagccggttg cgcgcccctc tccgccaaac gcataacccg cgagaaggcg aaggagcgag 1860

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg 1920

1861 tgactgagcg acgcgagcca gcaagccgac gccgctcgcc atagtcgagt gagtttccgc 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg agcaaaaggc 1980

1921 cattatgcca ataggtgtct tagtccccta ttgcgtcctt tcttgtacac tcgttttccg 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc 2040

1981 gtcgttttcc ggtccttggc atttttccgg cgcaacgacc gcaaaaaggt atccgaggcg 2040

.......... .......... .......... ........ pBR322_origin ........

.......... .......... .......... .......... .......... ..........

2041 ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga 2100

2041 gggggactgc tcgtagtgtt tttagctgcg agttcagtct ccaccgcttt gggctgtcct 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc 2160

2101 gatatttcta tggtccgcaa agggggacct tcgagggagc acgcgagagg acaaggctgg 2160

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat 2220

2161 gacggcgaat ggcctatgga caggcggaaa gagggaagcc cttcgcaccg cgaaagagta 2220

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2221 agctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg 2280

2221 tcgagtgcga catccataga gtcaagccac atccagcaag cgaggttcga cccgacacac 2280

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2281 cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc 2340

2281 gtgcttgggg ggcaagtcgg gctggcgacg cggaataggc cattgatagc agaactcagg 2340

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2341 aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga 2400

2341 ttgggccatt ctgtgctgaa tagcggtgac cgtcgtcggt gaccattgtc ctaatcgtct 2400

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact 2460

2401 cgctccatac atccgccacg atgtctcaag aacttcacca ccggattgat gccgatgtga 2460

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2461 agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt 2520

2461 tcttcctgtc ataaaccata gacgcgagac gacttcggtc aatggaagcc tttttctcaa 2520

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag 2580

2521 ccatcgagaa ctaggccgtt tgtttggtgg cgaccatcgc caccaaaaaa acaaacgttc 2580

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2581 cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg 2640

2581 gtcgtctaat gcgcgtcttt ttttcctaga gttcttctag gaaactagaa aagatgcccc 2640

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2641 tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa 2700

2641 agactgcgag tcaccttgct tttgagtgca attccctaaa accagtactc taatagtttt 2700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata 2760

2701 tcctagaagt ggatctagga aaatttaatt tttacttcaa aatttagtta gatttcatat 2760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 tatgagtaac ctgaggctat ggcagggcct gccgccccga cgttggctgc gagccctggg 2820

2761 atactcattg gactccgata ccgtcccgga cggcggggct gcaaccgacg ctcgggaccc 2820

.......... .......... .......... TK_PA_term .......... ..........

.......... .......... .......... .......... .......... ..........

2821 ccttcacccg aacttggggg gtggggtggg gaaaaggaag aaacgcgggc gtattggccc 2880

2821 ggaagtgggc ttgaaccccc caccccaccc cttttccttc tttgcgcccg cataaccggg 2880

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 caatggggtc tcggtggggt atcgacagag tgccagccct gggaccgaac cccgcgttta 2940

2881 gttaccccag agccacccca tagctgtctc acggtcggga ccctggcttg gggcgcaaat 2940

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 tgaacaaacg acccaacacc gtgcgtttta ttctgtcttt ttattgccgt catagcgcgg 3000

2941 acttgtttgc tgggttgtgg cacgcaaaat aagacagaaa aataacggca gtatcgcgcc 3000

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gttccttccg gtattgtctc cttccgtgtt tcagttagcc tccccctagg gtgggcgaag 3060

3001 caaggaaggc cataacagag gaaggcacaa agtcaatcgg agggggatcc cacccgcttc 3060

.......... .......... TK_PA_term .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aactccagca tgagatcccc gcgctggagg atcatccagc cggcgtcccg gaaaacgatt 3120

3061 ttgaggtcgt actctagggg cgcgacctcc tagtaggtcg gccgcagggc cttttgctaa 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ccgaagccca acctttcata gaaggcggcg gtggaatcga aatctcgtga tggcaggttg 3180

3121 ggcttcgggt tggaaagtat cttccgccgc caccttagct ttagagcact accgtccaac 3180

.......... .......... .......... .......... .......... ..........

.......... ......... BstBI(3203) ......... .......... ..........

3181 ggcgtcgctt ggtcggttca tttcgaaccc cagagtcccg ctcagaagaa ctcgtcaaga 3240

3181 ccgcagcgaa ccagccaagt aaagcttggg gtctcagggc gagtcttctt gagcagttct 3240

.......... .......... .......... .......... ... ORF 3 Neomycin(variant)

.......... .......... .......... .......... .......... ..........

3241 aggcgataga aggcgatgcg ctgcgaatcg ggagcggcga taccgtaaag cacgaggaag 3300

3241 tccgctatct tccgctacgc gacgcttagc cctcgccgct atggcatttc gtgctccttc 3300

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 cggtcagccc attcgccgcc aagctcttca gcaatatcac gggtagccaa cgctatgtcc 3360

3301 gccagtcggg taagcggcgg ttcgagaagt cgttatagtg cccatcggtt gcgatacagg 3360

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 tgatagcggt ccgccacacc cagccggcca cagtcgatga atccagaaaa gcggccattt 3420

3361 actatcgcca ggcggtgtgg gtcggccggt gtcagctact taggtctttt cgccggtaaa 3420

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 tccaccatga tattcggcaa gcaggcatcg tcatgggtca cgacgagatc ctcgccgtcg 3480

3421 aggtggtact ataagccgtt cgtccgtagc agtacccagt gctgctctag gagcggcagc 3480

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ggcatgctcg ccttgagcct ggcgaacagt tcggctggcg cgagcccctg atgctcttcg 3540

3481 ccgtacgagc ggaactcgga ccgcttgtca agccgaccgc gctcggggac tacgagaagc 3540

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 tccagatcat cctgatcgac aagaccggct tccatccgag tacgtgctcg ctcgatgcga 3600

3541 aggtctagta ggactagctg ttctggccga aggtaggctc atgcacgagc gagctacgct 3600

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 tgtttcgctt ggtggtcgaa tgggcaggta gccggatcaa gcgtatgcag ccgccgcatt 3660

3601 acaaagcgaa ccaccagctt acccgtccat cggcctagtt cgcatacgtc ggcggcgtaa 3660

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gcatcagcca tgatggatac tttctcggca ggagcaaggt gagatgacag gagatcctgc 3720

3661 cgtagtcggt actacctatg aaagagccgt cctcgttcca ctctactgtc ctctaggacg 3720

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 cccggcactt cgcccaatag cagccagtcc cttcccgctt cagtgacaac gtcgagcaca 3780

3721 gggccgtgaa gcgggttatc gtcggtcagg gaagggcgaa gtcactgttg cagctcgtgt 3780

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 gctgcgcaag gaacgcccgt cgtggccagc cacgatagcc gcgctgcctc gtcttgcagt 3840

3781 cgacgcgttc cttgcgggca gcaccggtcg gtgctatcgg cgcgacggag cagaacgtca 3840

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... NarI(3885) ..........

3841 tcattcaggg caccggacag gtcggtcttg acaaaaagaa ccgggcgccc ctgcgctgac 3900

3841 agtaagtccc gtggcctgtc cagccagaac tgtttttctt ggcccgcggg gacgcgactg 3900

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3901 agccggaaca cggcggcatc agagcagccg attgtctgtt gtgcccagtc atagccgaat 3960

3901 tcggccttgt gccgccgtag tctcgtcggc taacagacaa cacgggtcag tatcggctta 3960

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

3961 agcctctcca cccaagcggc cggagaacct gcgtgcaatc catcttgttc aatcatactc 4020

3961 tcggagaggt gggttcgccg gcctcttgga cgcacgttag gtagaacaag ttagtatgag 4020

.......... ... ORF 3 Neomycin(variant) ... .......... ..........

.......... .......... .......... .......... .......... ..........

4021 ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag cggatacata 4080

4021 aaggaaaaag ttataataac ttcgtaaata gtcccaataa cagagtactc gcctatgtat 4080

.......... .......... .......... .......... AmpR_pro ..........

.......... .......... .......... .......... .......... ..........

4081 tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg 4140

4081 aaacttacat aaatcttttt atttgtttat ccccaaggcg cgtgtaaagg ggcttttcac 4140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

4141 ccacctgaac gaagcatctg tgcttcattt tgtagaacaa aaatgcaacg cgagagcgct 4200

4141 ggtggacttg cttcgtagac acgaagtaaa acatcttgtt tttacgttgc gctctcgcga 4200

.......... .......... ....... 2micron2_origin ....... ..........

.......... .......... .......... .......... .......... ..........

4201 aatttttcaa acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgaaagc 4260

4201 ttaaaaagtt tgtttcttag actcgacgta aaaatgtctt gtctttacgt tgcgctttcg 4260

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4261 gctattttac caacgaagaa tctgtgcttc atttttgtaa aacaaaaatg caacgcgaga 4320

4261 cgataaaatg gttgcttctt agacacgaag taaaaacatt ttgtttttac gttgcgctct 4320

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4321 gcgctaattt ttcaaacaaa gaatctgagc tgcattttta cagaacagaa atgcaacgcg 4380

4321 cgcgattaaa aagtttgttt cttagactcg acgtaaaaat gtcttgtctt tacgttgcgc 4380

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4381 agagcgctat tttaccaaca aagaatctat acttcttttt tgttctacaa aaatgcatcc 4440

4381 tctcgcgata aaatggttgt ttcttagata tgaagaaaaa acaagatgtt tttacgtagg 4440

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4441 cgagagcgct atttttctaa caaagcatct tagattactt tttttctcct ttgtgcgctc 4500

4441 gctctcgcga taaaaagatt gtttcgtaga atctaatgaa aaaaagagga aacacgcgag 4500

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4501 tataatgcag tctcttgata actttttgca ctgtaggtcc gttaaggtta gaagaaggct 4560

4501 atattacgtc agagaactat tgaaaaacgt gacatccagg caattccaat cttcttccga 4560

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4561 actttggtgt ctattttctc ttccataaaa aaagcctgac tccacttccc gcgtttactg 4620

4561 tgaaaccaca gataaaagag aaggtatttt tttcggactg aggtgaaggg cgcaaatgac 4620

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4621 attactagcg aagctgcggg tgcatttttt caagataaag gcatccccga ttatattcta 4680

4621 taatgatcgc ttcgacgccc acgtaaaaaa gttctatttc cgtaggggct aatataagat 4680

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4681 taccgatgtg gattgcgcat actttgtgaa cagaaagtga tagcgttgat gattcttcat 4740

4681 atggctacac ctaacgcgta tgaaacactt gtctttcact atcgcaacta ctaagaagta 4740

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4741 tggtcagaaa attatgaacg gtttcttcta ttttgtctct atatactacg tataggaaat 4800

4741 accagtcttt taatacttgc caaagaagat aaaacagaga tatatgatgc atatccttta 4800

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4801 gtttacattt tcgtattgtt ttcgattcac tctatgaata gttcttacta caattttttt 4860

4801 caaatgtaaa agcataacaa aagctaagtg agatacttat caagaatgat gttaaaaaaa 4860

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4861 gtctaaagag taatactaga gataaacata aaaaatgtag aggtcgagtt tagatgcaag 4920

4861 cagatttctc attatgatct ctatttgtat tttttacatc tccagctcaa atctacgttc 4920

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4921 ttcaaggagc gaaaggtgga tgggtaggtt atatagggat atagcacaga gatatatagc 4980

4921 aagttcctcg ctttccacct acccatccaa tatatcccta tatcgtgtct ctatatatcg 4980

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

4981 aaagagatac ttttgagcaa tgtttgtgga agcggtattc gcaatatttt agtagctcgt 5040

4981 tttctctatg aaaactcgtt acaaacacct tcgccataag cgttataaaa tcatcgagca 5040

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5041 tacagtccgg tgcgtttttg gttttttgaa agtgcgtctt cagagcgctt ttggttttca 5100

5041 atgtcaggcc acgcaaaaac caaaaaactt tcacgcagaa gtctcgcgaa aaccaaaagt 5100

.......... ....... 2micron2_origin ....... .......... ..........

.......... .......... .......... .......... .......... ..........

5101 aaagcgctct gaagttccta tactttctag agaataggaa cttcggaata ggaacttcaa 5160

5101 tttcgcgaga cttcaaggat atgaaagatc tcttatcctt gaagccttat ccttgaagtt 5160

2micron2_origin .... .......... FRT .......... ..........

.......... .......... .......... .......... .......... ..........

5161 agcgtttccg aaaacgagcg cttccgaaaa tgcaacgcga gctgcgcaca tacagctcac 5220

5161 tcgcaaaggc ttttgctcgc gaaggctttt acgttgcgct cgacgcgtgt atgtcgagtg 5220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5221 tgttcacgtc gcacctatat ctgcgtgttg cctgtatata tatatacatg agaagaacgg 5280

5221 acaagtgcag cgtggatata gacgcacaac ggacatatat atatatgtac tcttcttgcc 5280

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5281 catagtgcgt gtttatgctt aaatgcgtac ttatatgcgt ctatttatgt aggatgaaag 5340

5281 gtatcacgca caaatacgaa tttacgcatg aatatacgca gataaataca tcctactttc 5340

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5341 gtagtctagt acctcctgtg atattatccc attccatgcg gggtatcgta tgcttccttc 5400

5341 catcagatca tggaggacac tataataggg taaggtacgc cccatagcat acgaaggaag 5400

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5401 agcactaccc tttagctgtt ctatatgctg ccactcctca attggattag tctcatcctt 5460

5401 tcgtgatggg aaatcgacaa gatatacgac ggtgaggagt taacctaatc agagtaggaa 5460

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5461 caatgctatc atttcctttg atattggatc atattaagaa accattatta tcatgacatt 5520

5461 gttacgatag taaaggaaac tataacctag tataattctt tggtaataat agtactgtaa 5520

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5521 aacctataaa aataggcgta tcacgaggcc ctttcgtctc gcgcgtttcg gtgatgacgg 5580

5521 ttggatattt ttatccgcat agtgctccgg gaaagcagag cgcgcaaagc cactactgcc 5580

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5581 tgaaaacctc tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc 5640

5581 acttttggag actgtgtacg tcgagggcct ctgccagtgt cgaacagaca ttcgcctacg 5640

.......... pGEX_3_pri .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5641 cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct 5700

5641 gccctcgtct gttcgggcag tcccgcgcag tcgcccacaa ccgcccacag ccccgaccga 5700

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5701 taactatgcg gcatcagagc agattgtact gagagtgcac catagatcaa cgacattact 5760

5701 attgatacgc cgtagtctcg tctaacatga ctctcacgtg gtatctagtt gctgtaatga 5760

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5761 atatatataa tataggaagc atttaataga acagcatcgt aatatatgtg tactttgcag 5820

5761 tatatatatt atatccttcg taaattatct tgtcgtagca ttatatacac atgaaacgtc 5820

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5821 ttatgacgcc agatggcagt agtggaagat attctttatt gaaaaatagc ttgtcacctt 5880

5821 aatactgcgg tctaccgtca tcaccttcta taagaaataa ctttttatcg aacagtggaa 5880

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

5881 acgtacaatc ttgatccgga gcttttcttt ttttgccgat taagaattaa ttcggtcgaa 5940

5881 tgcatgttag aactaggcct cgaaaagaaa aaaacggcta attcttaatt aagccagctt 5940

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... PmlI(5990) ..........

5941 aaaagaaaag gagagggcca agagggaggg cattggtgac tattgagcac gtgagtatac 6000

5941 ttttcttttc ctctcccggt tctccctccc gtaaccactg ataactcgtg cactcatatg 6000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6001 gtgattaagc acacaaaggc agcttggagt atgtctgtta ttaatttcac aggtagttct 6060

6001 cactaattcg tgtgtttccg tcgaacctca tacagacaat aattaaagtg tccatcaaga 6060

.......... .......... .......... .......... ORF 1 TRP1 ..........

.......... .......... .......... .......... .......... ..........

6061 ggtccattgg tgaaagtttg cggcttgcag agcacagagg ccgcagaatg tgctctagat 6120

6061 ccaggtaacc actttcaaac gccgaacgtc tcgtgtctcc ggcgtcttac acgagatcta 6120

.......... .......... ORF 1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6121 tccgatgctg acttgctggg tattatatgt gtgcccaata gaaagagaac aattgacccg 6180

6121 aggctacgac tgaacgaccc ataatataca cacgggttat ctttctcttg ttaactgggc 6180

.......... .......... ORF 1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6181 gttattgcaa ggaaaatttc aagtcttgta aaagcatata aaaatagttc aggcactccg 6240

6181 caataacgtt ccttttaaag ttcagaacat tttcgtatat ttttatcaag tccgtgaggc 6240

.......... .......... ORF 1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6241 aaatacttgg ttggcgtgtt tcgtaatcaa cctaaggagg atgttttggc tctggtcaat 6300

6241 tttatgaacc aaccgcacaa agcattagtt ggattcctcc tacaaaaccg agaccagtta 6300

.......... .......... ORF 1 TRP1 .......... .......... ..........

......... EcoRV(6315) ......... .......... .......... ..........

6301 gattacggca ttgatatcgt ccaactgcat ggagatgagt cgtggcaaga ataccaagag 6360

6301 ctaatgccgt aactatagca ggttgacgta cctctactca gcaccgttct tatggttctc 6360

.......... .......... ORF 1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6361 ttcctcggtt tgccagttat taaaagactc gtatttccaa aagactgcaa catactactc 6420

6361 aaggagccaa acggtcaata attttctgag cataaaggtt ttctgacgtt gtatgatgag 6420

.......... .......... ORF 1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6421 agtgcagctt cacagaaacc tcattcgttt attcccttgt ttgattcaga agcaggtggg 6480

6421 tcacgtcgaa gtgtctttgg agtaagcaaa taagggaaca aactaagtct tcgtccaccc 6480

.......... .......... ORF 1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6481 acaggtgaac ttttggattg gaactcgatt tctgactggg ttggaaggca agagagcccc 6540

6481 tgtccacttg aaaacctaac cttgagctaa agactgaccc aaccttccgt tctctcgggg 6540

.......... .......... ORF 1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6541 gaaagcttac attttatgtt agctggtgga ctgacgccag aaaatgttgg tgatgcgctt 6600

6541 ctttcgaatg taaaatacaa tcgaccacct gactgcggtc ttttacaacc actacgcgaa 6600

.......... .......... ORF 1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6601 agattaaatg gcgttattgg tgttgatgta agcggaggtg tggagacaaa tggtgtaaaa 6660

6601 tctaatttac cgcaataacc acaactacat tcgcctccac acctctgttt accacatttt 6660

.......... .......... ORF 1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6661 gactctaaca aaatagcaaa tttcgtcaaa aatgctaaga aataggttat tactgagtag 6720

6661 ctgagattgt tttatcgttt aaagcagttt ttacgattct ttatccaata atgactcatc 6720

.......... .......... ORF 1 TRP1 .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6721 tatttattta agtattgttt gtgcacttgc cgatctatgc ggtgtgaaat accgcacaga 6780

6721 ataaataaat tcataacaaa cacgtgaacg gctagatacg ccacacttta tggcgtgtct 6780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6781 tgcgtaagga gaaaataccg catcaggaaa ttgtaaacgt taatattttg ttaaaattcg 6840

6781 acgcattcct cttttatggc gtagtccttt aacatttgca attataaaac aattttaagc 6840

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6841 cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc 6900

6841 gcaatttaaa aacaatttag tcgagtaaaa aattggttat ccggctttag ccgttttagg 6900

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

6901 cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga 6960

6901 gaatatttag ttttcttatc tggctctatc ccaactcaca acaaggtcaa accttgttct 6960

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

6961 gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg 7020

6961 caggtgataa tttcttgcac ctgaggttgc agtttcccgc tttttggcag atagtcccgc 7020

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7021 atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag 7080

7021 taccgggtga tgcacttggt agtgggatta gttcaaaaaa ccccagctcc acggcatttc 7080

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7081 cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga 7140

7081 gtgatttagc cttgggattt ccctcggggg ctaaatctcg aactgcccct ttcggccgct 7140

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7141 acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg 7200

7141 tgcaccgctc tttccttccc ttctttcgct ttcctcgccc gcgatcccgc gaccgttcac 7200

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7201 tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg 7260

7201 atcgccagtg cgacgcgcat tggtggtgtg ggcggcgcga attacgcggc gatgtcccgc 7260

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

7261 cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgat...... .......... 7304

7261 gcaggtaagc ggtaagtccg acgcgttgac aacccttccc gcta...... .......... 7304

.......... .......... .......... .......... .......... ..........