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pGEM-LIN28
Gene/insert name: Homo sapiens lin-28 homolog (C. elegans)
Alternative names: LIN28
Insert size (bp): 600
GenBank/Entrez ID of insert: NM_024674
Gene/insert aliases: LIN28, CSDD1, LIN-28, LIN28A, ZCCHC1, FLJ12457
Species of gene(s): H. sapiens (human)
Vector backbone: pGEM-T Easy (Search Vector Database)
Backbone manufacturer: Promega
Type of vector: Bacterial expression
Backbone size (bp): 3662
5' Sequencing primer: T7 (List of Sequencing Primers)
3' Sequencing primer: M13 Reverse
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: James A Thomson
Comments: This plasmid contains the Lin28 sequence that was cloned into the lentivirus plasmid and used in cell reprogramming.
Article: Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu J et al. (Science. 2007 Nov 20. ():. Pubmed)