Sequence of pGEM-LIN28

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Sequence of pGEM-LIN28

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Sequence of pGEM-LIN28 : 3662 BP; 927 A; 920 C; 974 G; 840 T; 1 other;

.......... AatII(20) .......... .......... SacII(49) ..........

1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat 60

1 cccgcttaac ccgggctgca gcgtacgagg gccggcggta ccgccggcgc ccttaagcta 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tatcgccatg ggctccgtgt ccaaccagca gtttgcaggt ggctgcgcca aggcggcaga 120

61 atagcggtac ccgaggcaca ggttggtcgt caaacgtcca ccgacgcggt tccgccgtct 120

.......... .......... ......... Orf frame 2 ......... ..........

.......... .......... .......... Smal(155) .......... ..........

121 agaggcgccc gaggaggcgc cggaggacgc ggcccgggcg gcggacgagc ctcagctgct 180

121 tctccgcggg ctcctccgcg gcctcctgcg ccgggcccgc cgcctgctcg gagtcgacga 180

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 gcacggtgcg ggcatctgta agtggttcaa cgtgcgcatg gggttcggct tcctgtccat 240

181 cgtgccacgc ccgtagacat tcaccaagtt gcacgcgtac cccaagccga aggacaggta 240

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 gaccgcccgc gccggggtcg cgctcgaccc cccagtggat gtctttgtgc accagagtaa 300

241 ctggcgggcg cggccccagc gcgagctggg gggtcaccta cagaaacacg tggtctcatt 300

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 gctgcacatg gaagggttcc ggagcttgaa ggagggtgag gcagtggagt tcacctttaa 360

301 cgacgtgtac cttcccaagg cctcgaactt cctcccactc cgtcacctca agtggaaatt 360

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gaagtcagcc aagggtctgg aatccatccg tgtcaccgga cctggtggag tattctgtat 420

361 cttcagtcgg ttcccagacc ttaggtaggc acagtggcct ggaccacctc ataagacata 420

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 tgggagtgag aggcggccaa aaggaaagag catgcagaag cgcagatcaa aaggagacag 480

421 accctcactc tccgccggtt ttcctttctc gtacgtcttc gcgtctagtt ttcctctgtc 480

.......... ......... Orf frame 2 ......... .......... ..........

.......... Xbal(499) .......... .......... .......... ..........

481 gtgctacaac tgtggaggtc tagatcatca tgccaaggaa tgcaagctgc caccccagcc 540

481 cacgatgttg acacctccag atctagtagt acggttcctt acgttcgacg gtggggtcgg 540

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 caagaagtgc cacttctgcc agagcatcag ccatatggta gcctcatgtc cgctgaaggc 600

541 gttcttcacg gtgaagacgg tctcgtagtc ggtataccat cggagtacag gcgacttccg 600

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 ccagcagggc cctagtgcac agggaaagcc aacctacttt cgagaggaag aagaagaaat 660

601 ggtcgtcccg ggatcacgtg tccctttcgg ttggatgaaa gctctccttc ttcttcttta 660

.......... ......... Orf frame 2 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 ccacagccct accctgctcc cggaggcaca gaattgagtt taaacnaatc actagtgaat 720

661 ggtgtcggga tgggacgagg gcctccgtgt cttaactcaa atttgnttag tgatcactta 720

......... Orf frame 2 ......... .......... .......... ..........

..... PstI(735),SalI(737) ..... SacI(756) .......... ..........

721 tcgcggccgc ctgcaggtcg accatatggg agagctccca acgcgttgga tgcatagctt 780

721 agcgccggcg gacgtccagc tggtataccc tctcgagggt tgcgcaacct acgtatcgaa 780

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 gagtattcta tagtgtcacc taaatagctt ggcgtaatca tggtcatagc tgtttcctgt 840

781 ctcataagat atcacagtgg atttatcgaa ccgcattagt accagtatcg acaaaggaca 840

.......... Sp6_primer .......... ...... M13_reverse_primer ......

.......... .......... .......... .......... .......... ..........

841 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa 900

841 cactttaaca ataggcgagt gttaaggtgt gttgtatgct cggccttcgt atttcacatt 900

M13_pUC_rev_primer .. .......... ......... lac_promoter .........

.......... .......... .......... .......... .......... ..........

901 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc 960

901 tcggacccca cggattactc actcgattga gtgtaattaa cgcaacgcga gtgacgggcg 960

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

961 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag 1020

961 aaaggtcagc cctttggaca gcacggtcga cgtaattact tagccggttg cgcgcccctc 1020

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1021 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt 1080

1021 tccgccaaac gcataacccg cgagaaggcg aaggagcgag tgactgagcg acgcgagcca 1080

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1081 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga 1140

1081 gcaagccgac gccgctcgcc atagtcgagt gagtttccgc cattatgcca ataggtgtct 1140

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1141 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg 1200

1141 tagtccccta ttgcgtcctt tcttgtacac tcgttttccg gtcgttttcc ggtccttggc 1200

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa 1260

1201 atttttccgg cgcaacgacc gcaaaaaggt atccgaggcg gggggactgc tcgtagtgtt 1260

.......... .......... ........ pBR322_origin ........ ..........

.......... .......... .......... .......... .......... ..........

1261 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt 1320

1261 tttagctgcg agttcagtct ccaccgcttt gggctgtcct gatatttcta tggtccgcaa 1320

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1321 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct 1380

1321 agggggacct tcgagggagc acgcgagagg acaaggctgg gacggcgaat ggcctatgga 1380

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1381 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct 1440

1381 caggcggaaa gagggaagcc cttcgcaccg cgaaagagta tcgagtgcga catccataga 1440

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1441 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc 1500

1441 gtcaagccac atccagcaag cgaggttcga cccgacacac gtgcttgggg ggcaagtcgg 1500

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1501 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt 1560

1501 gctggcgacg cggaataggc cattgatagc agaactcagg ttgggccatt ctgtgctgaa 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc 1620

1561 tagcggtgac cgtcgtcggt gaccattgtc ctaatcgtct cgctccatac atccgccacg 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat 1680

1621 atgtctcaag aacttcacca ccggattgat gccgatgtga tcttcttgtc ataaaccata 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa 1740

1681 gacgcgagac gacttcggtc aatggaagcc tttttctcaa ccatcgagaa ctaggccgtt 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa 1800

1741 tgtttggtgg cgaccatcgc caccaaaaaa acaaacgttc gtcgtctaat gcgcgtcttt 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga 1860

1801 ttttcctaga gttcttctag gaaactagaa aagatgcccc agactgcgag tcaccttgct 1860

........ pBR322_origin ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1861 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 1920

1861 tttgagtgca attccctaaa accagtactc taatagtttt tcctagaagt ggatctagga 1920

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1921 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 1980

1921 aaatttaatt tttacttcaa aatttagtta gatttcatat atactcattt gaaccagact 1980

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1981 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 2040

1981 gtcaatggtt acgaattagt cactccgtgg atagagtcgc tagacagata aagcaagtag 2040

.......... .......... .... Orf frame 1 Ampicillin .... ..........

.......... .......... .......... .......... .......... ..........

2041 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 2100

2041 gtatcaacgg actgaggggc agcacatcta ttgatgctat gccctcccga atggtagacc 2100

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2101 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 2160

2101 ggggtcacga cgttactatg gcgctctggg tgcgagtggc cgaggtctaa atagtcgtta 2160

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 2220

2161 tttggtcggt cggccttccc ggctcgcgtc ttcaccagga cgttgaaata ggcggaggta 2220

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 2280

2221 ggtcagataa ttaacaacgg cccttcgatc tcattcatca agcggtcaat tatcaaacgc 2280

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2281 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 2340

2281 gttgcaacaa cggtaacgat gtccgtagca ccacagtgcg agcagcaaac cataccgaag 2340

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 2400

2341 taagtcgagg ccaagggttg ctagttccgc tcaatgtact agggggtaca acacgttttt 2400

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 2460

2401 tcgccaatcg aggaagccag gaggctagca acagtcttca ttcaaccggc gtcacaatag 2460

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 2520

2461 tgagtaccaa taccgtcgtg acgtattaag agaatgacag tacggtaggc attctacgaa 2520

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 2580

2521 aagacactga ccactcatga gttggttcag taagactctt atcacatacg ccgctggctc 2580

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 2640

2581 aacgagaacg ggccgcagtt atgccctatt atggcgcggt gtatcgtctt gaaattttca 2640

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 2700

2641 cgagtagtaa ccttttgcaa gaagccccgc ttttgagagt tcctagaatg gcgacaactc 2700

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 2760

2701 taggtcaagc tacattgggt gagcacgtgg gttgactaga agtcgtagaa aatgaaagtg 2760

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 2820

2761 gtcgcaaaga cccactcgtt tttgtccttc cgttttacgg cgttttttcc cttattcccg 2820

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 2880

2821 ctgtgccttt acaacttatg agtatgagaa ggaaaaagtt ataataactt cgtaaatagt 2880

.... Orf frame 1 Ampicillin .... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 2940

2881 cccaataaca gagtactcgc ctatgtataa acttacataa atctttttat ttgtttatcc 2940

........ AmpR_promoter ........ .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ggttccgcgc acatttcccc gaaaagtgcc acctgatgcg gtgtgaaata ccgcacagat 3000

2941 ccaaggcgcg tgtaaagggg cttttcacgg tggactacgc cacactttat ggcgtgtcta 3000

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt taaaattcgc 3060

3001 cgcattcctc ttttatggcg tagtccttta acattcgcaa ttataaaaca attttaagcg 3060

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg gcaaaatccc 3120

3061 caatttaaaa acaatttagt cgagtaaaaa attggttatc cggctttagc cgttttaggg 3120

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt ggaacaagag 3180

3121 aatatttagt tttcttatct ggctctatcc caactcacaa caaggtcaaa ccttgttctc 3180

.......... .......... .......... .......... f1_origin ..........

.......... .......... .......... .......... .......... ..........

3181 tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct atcagggcga 3240

3181 aggtgataat ttcttgcacc tgaggttgca gtttcccgct ttttggcaga tagtcccgct 3240

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt gccgtaaagc 3300

3241 accgggtgat gcacttggta gtgggattag ttcaaaaaac cccagctcca cggcatttcg 3300

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa agccggcgaa 3360

3301 tgatttagcc ttgggatttc cctcgggggc taaatctcga actgcccctt tcggccgctt 3360

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc tggcaagtgt 3420

3361 gcaccgctct ttccttccct tctttcgctt tcctcgcccg cgatcccgcg accgttcaca 3420

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc tacagggcgc 3480

3421 tcgccagtgc gacgcgcatt ggtggtgtgg gcggcgcgaa ttacgcggcg atgtcccgcg 3480

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3481 gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg 3540

3481 caggtaagcg gtaagtccga cgcgttgaca acccttcccg ctagccacgc ccggagaagc 3540

.......... .......... .......... lacZ_a .......... ..........

.......... .......... .......... .......... .......... ..........

3541 ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg ggtaacgcca 3600

3541 gataatgcgg tcgaccgctt tccccctaca cgacgttccg ctaattcaac ccattgcggt 3600

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata cgactcacta 3660

3601 cccaaaaggg tcagtgctgc aacattttgc tgccggtcac ttaacattat gctgagtgat 3660

. M13_pUC_fwd_primer lacZ_a M13_forward20_primer . ..........

.......... .......... .......... .......... .......... ..........

3661 ta........ .......... .......... .......... .......... .......... 3662

3661 at........ .......... .......... .......... .......... .......... 3662

.......... .......... .......... .......... .......... ..........