spsp>pGEM-Nanog plasmid sequence 3941 bp gggcgaattgggcccgacgtcgcatgctcccggccgccatggcggccgcgggaattcgattcatcttcacacgtcttcag gttgcatgttcatggagtagtttaggaataaatccatggtttgtggagtactaaaatacctagtggtctgctgtattaca ttaaggccttccccagcagcttccaaggcagcctccaagtcactggcaggagaatttggctggaactgcatgcaggactg cagagattcctctccacagttatagaagggactgttccaggcctgattgttccaggattgggtgcaccaggtctgagtgt tccaggagtggttgctccaggactggatgttctgggtctggttgctccaggttgaattgttccaggtctggttgctccac attggaaggttcccagtcgggttcaccaggcatccctggtggtaggaagagtaaaggctggggtaggtaggtgctgaggc cttctgcgtcacaccattgctattcttcggccagttgtttttctgccacctcttagatttcattctctggttctggaacc aggtcttcacctgtttgtagctgaggttcaggatgttggagagttcttgcatctgctggaggctgaggtatttctgtctc tgaaatctatcattgagtacacacagctgggtggaagagaacacagttctggtcttctgtttcttgaccgggaccttgtc ttccttttttgcgacactcttctctgcagaagtgggttgtttgcctttgggactggtggaagaatcagggctgtcctgaa taagcagatccatggaggaaggaagaggagagacagtctccgtgtgaggcatctcagcagaagacatttgcaaggatgga tagttttcttcaggcccacaaatcacaggcataggtgaagattctttacagtcggatgcttcaaagcaaggcaagctttg gggacaagctggatccacactcatgaatcactagtgaattcgcggccgcctgcaggtcgaccatatgggagagctcccaa cgcgttggatgcatagcttgagtattctatagtgtcacctaaatagcttggcgtaatcatggtcatagctgtttcctgtg tgaaattgttatccgctcacaattccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagt gagctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaa tcggccaacgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactcgctgcgctcggtc gttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaa gaacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcc cccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgttt ccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggg aagcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgc acgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgactta tcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtg gcctaactacggctacactagaagaacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttg gtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaa aaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggatttt ggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatat atgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatcc atagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgatacc gcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctg caactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgc aacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacg atcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagta agttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttt tctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaat acgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaa ggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcacc agcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatact catactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtattt agaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgatgcggtgtgaaataccgcacagatg cgtaaggagaaaataccgcatcaggaaattgtaagcgttaatattttgttaaaattcgcgttaaatttttgttaaatcag ctcattttttaaccaataggccgaaatcggcaaaatcccttataaatcaaaagaatagaccgagatagggttgagtgttg ttccagtttggaacaagagtccactattaaagaacgtggactccaacgtcaaagggcgaaaaaccgtctatcagggcgat ggcccactacgtgaaccatcaccctaatcaagttttttggggtcgaggtgccgtaaagcactaaatcggaaccctaaagg gagcccccgatttagagcttgacggggaaagccggcgaacgtggcgagaaaggaagggaagaaagcgaaaggagcgggcg ctagggcgctggcaagtgtagcggtcacgctgcgcgtaaccaccacacccgccgcgcttaatgcgccgctacagggcgcg tccattcgccattcaggctgcgcaactgttgggaagggcgatcggtgcgggcctcttcgctattacgccagctggcgaaa gggggatgtgctgcaaggcgattaagttgggtaacgccagggttttcccagtcacgacgttgtaaaacgacggccagtga attgtaatacgactcactata e pGEM-Nanog "Times New RomanspCeC Orf frame 1"Times New RomanspCeU T7_promoter"Times New RomanspCe+< Sp6_primer"Times New RomanspCɆeJ\M13_reverse_primer"Times New Romanspe[qM13_pUC_rev_primer"Times New RomanspCe lac_promoter"Times New RomanspCe< pBR322_origin"Times New RomanspCɆe3  Orf frame 1"Times New Romanspe3  Ampicillin"Times New RomanspCe] y  AmpR_promoter"Times New RomanspCen  f1_origin"Times New RomanspCɆeQlacZ_a"Times New Romanspe/EM13_pUC_fwd_primer"Times New RomanspCe>NM13_forward20_primer"Times New Roman) spApaI(14), AatII(20), SacII(49) "Times New Roman0e9Lp_hnXn+X spGHindIII(953), BamHI(971), SpeI(990), SalI(1016), NdeI(1023), SacI(1035)"Times New RomaneF3FGFGs sptest"Times New Romangertz=\a sptest"Times New RomanDe:p//a