Sequence of pGEM-Nanog

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Sequence of pGEM-Nanog

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Sequence of pGEM-Nanog : 3941 BP; 975 A; 944 C; 1024 G; 998 T; 0 other;

...... ApaI(14) AatII(20) ...... .......... SacII(49) ..........

1 gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggcggccgcg ggaattcgat 60

1 cccgcttaac ccgggctgca gcgtacgagg gccggcggta ccgccggcgc ccttaagcta 60

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

61 tcatcttcac acgtcttcag gttgcatgtt catggagtag tttaggaata aatccatggt 120

61 agtagaagtg tgcagaagtc caacgtacaa gtacctcatc aaatccttat ttaggtacca 120

.......... .......... ......... Orf frame 1 ......... ..........

.......... .......... .......... .......... .......... ..........

121 ttgtggagta ctaaaatacc tagtggtctg ctgtattaca ttaaggcctt ccccagcagc 180

121 aacacctcat gattttatgg atcaccagac gacataatgt aattccggaa ggggtcgtcg 180

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

181 ttccaaggca gcctccaagt cactggcagg agaatttggc tggaactgca tgcaggactg 240

181 aaggttccgt cggaggttca gtgaccgtcc tcttaaaccg accttgacgt acgtcctgac 240

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

241 cagagattcc tctccacagt tatagaaggg actgttccag gcctgattgt tccaggattg 300

241 gtctctaagg agaggtgtca atatcttccc tgacaaggtc cggactaaca aggtcctaac 300

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

301 ggtgcaccag gtctgagtgt tccaggagtg gttgctccag gactggatgt tctgggtctg 360

301 ccacgtggtc cagactcaca aggtcctcac caacgaggtc ctgacctaca agacccagac 360

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

361 gttgctccag gttgaattgt tccaggtctg gttgctccac attggaaggt tcccagtcgg 420

361 caacgaggtc caacttaaca aggtccagac caacgaggtg taaccttcca agggtcagcc 420

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

421 gttcaccagg catccctggt ggtaggaaga gtaaaggctg gggtaggtag gtgctgaggc 480

421 caagtggtcc gtagggacca ccatccttct catttccgac cccatccatc cacgactccg 480

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

481 cttctgcgtc acaccattgc tattcttcgg ccagttgttt ttctgccacc tcttagattt 540

481 gaagacgcag tgtggtaacg ataagaagcc ggtcaacaaa aagacggtgg agaatctaaa 540

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

541 cattctctgg ttctggaacc aggtcttcac ctgtttgtag ctgaggttca ggatgttgga 600

541 gtaagagacc aagaccttgg tccagaagtg gacaaacatc gactccaagt cctacaacct 600

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

601 gagttcttgc atctgctgga ggctgaggta tttctgtctc tgaaatctat cattgagtac 660

601 ctcaagaacg tagacgacct ccgactccat aaagacagag actttagata gtaactcatg 660

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

661 acacagctgg gtggaagaga acacagttct ggtcttctgt ttcttgaccg ggaccttgtc 720

661 tgtgtcgacc caccttctct tgtgtcaaga ccagaagaca aagaactggc cctggaacag 720

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

721 ttcctttttt gcgacactct tctctgcaga agtgggttgt ttgcctttgg gactggtgga 780

721 aaggaaaaaa cgctgtgaga agagacgtct tcacccaaca aacggaaacc ctgaccacct 780

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

781 agaatcaggg ctgtcctgaa taagcagatc catggaggaa ggaagaggag agacagtctc 840

781 tcttagtccc gacaggactt attcgtctag gtacctcctt ccttctcctc tctgtcagag 840

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

841 cgtgtgaggc atctcagcag aagacatttg caaggatgga tagttttctt caggcccaca 900

841 gcacactccg tagagtcgtc ttctgtaaac gttcctacct atcaaaagaa gtccgggtgt 900

.......... ......... Orf frame 1 ......... .......... ..........

.......... .......... .......... .......... .......... ..........

901 aatcacaggc ataggtgaag attctttaca gtcggatgct tcaaagcaag gcaagctttg 960

901 ttagtgtccg tatccacttc taagaaatgt cagcctacga agtttcgttc cgttcgaaac 960

.......... ......... Orf frame 1 ......... .......... ..........

.......... BamHI(971) SpeI(990) .......... .......... ..........

961 gggacaagct ggatccacac tcatgaatca ctagtgaatt cgcggccgcc tgcaggtcga 1020

961 ccctgttcga cctaggtgtg agtacttagt gatcacttaa gcgccggcgg acgtccagct 1020

......... Orf frame 1 ......... .......... .......... ..........

NdeI(1023) SacI(1035) .......... .......... .......... ..........

1021 ccatatggga gagctcccaa cgcgttggat gcatagcttg agtattctat agtgtcacct 1080

1021 ggtataccct ctcgagggtt gcgcaaccta cgtatcgaac tcataagata tcacagtgga 1080

.......... .......... .......... .......... .......... Sp6_primer

.......... .......... .......... .......... .......... ..........

1081 aaatagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac 1140

1081 tttatcgaac cgcattagta ccagtatcga caaaggacac actttaacaa taggcgagtg 1140

.......... ...... M13_reverse_primer .. M13_pUC_rev_primer ......

.......... .......... .......... .......... .......... ..........

1141 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt 1200

1141 ttaaggtgtg ttgtatgctc ggccttcgta tttcacattt cggaccccac ggattactca 1200

.......... ......... lac_promoter ......... .......... ..........

.......... .......... .......... .......... .......... ..........

1201 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc 1260

1201 ctcgattgag tgtaattaac gcaacgcgag tgacgggcga aaggtcagcc ctttggacag 1260

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1261 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg 1320

1261 cacggtcgac gtaattactt agccggttgc gcgcccctct ccgccaaacg cataacccgc 1320

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1321 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt 1380

1321 gagaaggcga aggagcgagt gactgagcga cgcgagccag caagccgacg ccgctcgcca 1380

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1381 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa 1440

1381 tagtcgagtg agtttccgcc attatgccaa taggtgtctt agtcccctat tgcgtccttt 1440

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

1441 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc 1500

1441 cttgtacact cgttttccgg tcgttttccg gtccttggca tttttccggc gcaacgaccg 1500

.......... .......... .......... .......... ........ pBR322_origin

.......... .......... .......... .......... .......... ..........

1501 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag 1560

1501 caaaaaggta tccgaggcgg ggggactgct cgtagtgttt ttagctgcga gttcagtctc 1560

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1561 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt 1620

1561 caccgctttg ggctgtcctg atatttctat ggtccgcaaa gggggacctt cgagggagca 1620

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1621 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg 1680

1621 cgcgagagga caaggctggg acggcgaatg gcctatggac aggcggaaag agggaagccc 1680

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1681 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg 1740

1681 ttcgcaccgc gaaagagtat cgagtgcgac atccatagag tcaagccaca tccagcaagc 1740

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1741 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg 1800

1741 gaggttcgac ccgacacacg tgcttggggg gcaagtcggg ctggcgacgc ggaataggcc 1800

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1801 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac 1860

1801 attgatagca gaactcaggt tgggccattc tgtgctgaat agcggtgacc gtcgtcggtg 1860

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1861 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg 1920

1861 accattgtcc taatcgtctc gctccataca tccgccacga tgtctcaaga acttcaccac 1920

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1921 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt 1980

1921 cggattgatg ccgatgtgat cttcttgtca taaaccatag acgcgagacg acttcggtca 1980

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

1981 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg 2040

1981 atggaagcct ttttctcaac catcgagaac taggccgttt gtttggtggc gaccatcgcc 2040

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2041 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc 2100

2041 accaaaaaaa caaacgttcg tcgtctaatg cgcgtctttt tttcctagag ttcttctagg 2100

.......... ........ pBR322_origin ........ .......... ..........

.......... .......... .......... .......... .......... ..........

2101 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt 2160

2101 aaactagaaa agatgcccca gactgcgagt caccttgctt ttgagtgcaa ttccctaaaa 2160

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2161 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt 2220

2161 ccagtactct aatagttttt cctagaagtg gatctaggaa aatttaattt ttacttcaaa 2220

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

2221 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag 2280

2221 atttagttag atttcatata tactcatttg aaccagactg tcaatggtta cgaattagtc 2280

.......... .......... .......... .......... .... Orf frame 1 Ampicillin

.......... .......... .......... .......... .......... ..........

2281 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt 2340

2281 actccgtgga tagagtcgct agacagataa agcaagtagg tatcaacgga ctgaggggca 2340

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2341 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc 2400

2341 gcacatctat tgatgctatg ccctcccgaa tggtagaccg gggtcacgac gttactatgg 2400

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2401 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc 2460

2401 cgctctgggt gcgagtggcc gaggtctaaa tagtcgttat ttggtcggtc ggccttcccg 2460

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2461 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg 2520

2461 gctcgcgtct tcaccaggac gttgaaatag gcggaggtag gtcagataat taacaacggc 2520

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2521 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac 2580

2521 ccttcgatct cattcatcaa gcggtcaatt atcaaacgcg ttgcaacaac ggtaacgatg 2580

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2581 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg 2640

2581 tccgtagcac cacagtgcga gcagcaaacc ataccgaagt aagtcgaggc caagggttgc 2640

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2641 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc 2700

2641 tagttccgct caatgtacta gggggtacaa cacgtttttt cgccaatcga ggaagccagg 2700

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2701 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact 2760

2701 aggctagcaa cagtcttcat tcaaccggcg tcacaatagt gagtaccaat accgtcgtga 2760

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2761 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc 2820

2761 cgtattaaga gaatgacagt acggtaggca ttctacgaaa agacactgac cactcatgag 2820

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2821 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat 2880

2821 ttggttcagt aagactctta tcacatacgc cgctggctca acgagaacgg gccgcagtta 2880

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2881 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc 2940

2881 tgccctatta tggcgcggtg tatcgtcttg aaattttcac gagtagtaac cttttgcaag 2940

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

2941 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac 3000

2941 aagccccgct tttgagagtt cctagaatgg cgacaactct aggtcaagct acattgggtg 3000

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3001 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa 3060

3001 agcacgtggg ttgactagaa gtcgtagaaa atgaaagtgg tcgcaaagac ccactcgttt 3060

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3061 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact 3120

3061 ttgtccttcc gttttacggc gttttttccc ttattcccgc tgtgccttta caacttatga 3120

.......... .... Orf frame 1 Ampicillin .... .......... ..........

.......... .......... .......... .......... .......... ..........

3121 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg 3180

3121 gtatgagaag gaaaaagtta taataacttc gtaaatagtc ccaataacag agtactcgcc 3180

.......... .......... .......... .......... ........ AmpR_promoter

.......... .......... .......... .......... .......... ..........

3181 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg 3240

3181 tatgtataaa cttacataaa tctttttatt tgtttatccc caaggcgcgt gtaaaggggc 3240

AmpR_promoter ...... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3241 aaaagtgcca cctgatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca 3300

3241 ttttcacggt ggactacgcc acactttatg gcgtgtctac gcattcctct tttatggcgt 3300

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3301 tcaggaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt gttaaatcag 3360

3301 agtcctttaa cattcgcaat tataaaacaa ttttaagcgc aatttaaaaa caatttagtc 3360

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3361 ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa aagaatagac 3420

3361 gagtaaaaaa ttggttatcc ggctttagcc gttttaggga atatttagtt ttcttatctg 3420

.......... .......... .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3421 cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa agaacgtgga 3480

3421 gctctatccc aactcacaac aaggtcaaac cttgttctca ggtgataatt tcttgcacct 3480

.......... .......... .......... f1_origin .......... ..........

.......... .......... .......... .......... .......... ..........

3481 ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac gtgaaccatc 3540

3481 gaggttgcag tttcccgctt tttggcagat agtcccgcta ccgggtgatg cacttggtag 3540

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3541 accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg 3600

3541 tgggattagt tcaaaaaacc ccagctccac ggcatttcgt gatttagcct tgggatttcc 3600

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3601 gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa aggaagggaa 3660

3601 ctcgggggct aaatctcgaa ctgccccttt cggccgcttg caccgctctt tccttccctt 3660

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3661 gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac 3720

3661 ctttcgcttt cctcgcccgc gatcccgcga ccgttcacat cgccagtgcg acgcgcattg 3720

.......... .......... f1_origin .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3721 caccacaccc gccgcgctta atgcgccgct acagggcgcg tccattcgcc attcaggctg 3780

3721 gtggtgtggg cggcgcgaat tacgcggcga tgtcccgcgc aggtaagcgg taagtccgac 3780

.......... f1_origin .......... .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3781 cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca gctggcgaaa 3840

3781 gcgttgacaa cccttcccgc tagccacgcc cggagaagcg ataatgcggt cgaccgcttt 3840

.......... .......... lacZ_a .......... .......... ..........

.......... .......... .......... .......... .......... ..........

3841 gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca gtcacgacgt 3900

3841 ccccctacac gacgttccgc taattcaacc cattgcggtc ccaaaagggt cagtgctgca 3900

.......... .......... lacZ_a .......... ...... M13_pUC_fwd_primer

.......... .......... .......... .......... .......... ..........

3901 tgtaaaacga cggccagtga attgtaatac gactcactat a......... .......... 3941

3901 acattttgct gccggtcact taacattatg ctgagtgata t......... .......... 3941

M13_pUC_fwd_primer M13_forward20_primer lacZ_a ..........