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Gene/insert name: Homo sapiens POU domain, class 5, transcription factor 1 (POU5F1)
Alternative names: POU5F1
Insert size (bp): 1100
GenBank/Entrez ID of insert: NM_002701
Gene/insert aliases: POU5F1, OCT3, OCT4, OTF3, OTF4, MGC22487
Species of gene(s): H. sapiens (human)
Vector backbone: pGEM-T Easy (Search Vector Database)
Backbone manufacturer: Promega
Type of vector: Bacterial expression
Backbone size (bp): 3000
5' Sequencing primer: M13 Reverse (List of Sequencing Primers)
3' Sequencing primer: T7
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: James A Thomson
Comments: This plasmid contains the Oct4 sequence that was cloned into the lentivirus plasmid and used in cell reprogramming.
Article: Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu J et al. (Science. 2007 Nov 20. ():. Pubmed)
Please acknowledge the principal investigator and cite this article if you use this plasmid in a publication.