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pGEM-Sox2
Gene/insert name: Homo sapiens SRY (sex determining region Y)-box 2 (SOX2)
Alternative names: Sox2
Insert size (bp): 1000
GenBank/Entrez ID of insert: NM_003106
Gene/insert aliases: SOX2, ANOP3, MCOPS3, MGC2413
Species of gene(s): H. sapiens (human)
Vector backbone: pGEM-T Easy (Search Vector Database)
Backbone manufacturer: Promega
Type of vector: Bacterial expression
Backbone size (bp): 3000
5' Sequencing primer: T7 (List of Sequencing Primers)
3' Sequencing primer: M13 Reverse
Bacteria resistance: Ampicillin
High or low copy: High Copy
Grow in standard E. coli @ 37C: Yes
Plasmid Provided In: DH5a
Principal Investigator: James A Thomson
Comments: This plasmid contains the Sox2 sequence that was cloned into the lentivirus plasmid and used in cell reprogramming.
Article: Induced Pluripotent Stem Cell Lines Derived from Human Somatic Cells. Yu J et al. (Science. 2007 Nov 20. ():. Pubmed)